Hcrt (NM_013179) Rat Tagged ORF Clone

CAT#: RR215222

Hcrt (Myc-DDK-tagged ORF) - Rat hypocretin (Hcrt), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hcrt
• Synonyms: orexin-A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215222 representing NM_013179
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTTCCTTCTACAAAGGTTCCCTGGGCCGCCGTGACGCTGCTGCTGCTGCTACTGCTGCCGCCGG
  CGCTGCTGTCGCTTGGGGTGGACGCGCAGCCTCTGCCCGACTGCTGTCGCCAGAAGACGTGTTCCTGCCG
  TCTCTACGAACTGTTGCACGGAGCTGGCAACCACGCCGCGGGCATCCTCACTCTGGGAAAGCGGCGACCT
  GGACCCCCAGGCCTCCAAGGACGGCTGCAGCGCCTCCTTCAGGCCAACGGTAACCACGCAGCTGGCATCC
  TGACCATGGGCCGCCGCGCAGGCGCAGAGCTAGAGCCATATCCCTGCCCTGGTCGCCGCTGTCCGACTGC
  AACCGCCACCGCTTTAGCGCCCCGGGGCGGATCCAGAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215222 representing NM_013179
  Red=Cloning site Green=Tags(s)
  
  MNLPSTKVPWAAVTLLLLLLLPPALLSLGVDAQPLPDCCRQKTCSCRLYELLHGAGNHAAGILTLGKRRP
  GPPGLQGRLQRLLQANGNHAAGILTMGRRAGAELEPYPCPGRRCPTATATALAPRGGSRV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013179
• ORF Size: 390 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013179.1, NM_013179.2, NP_037311.1
• RefSeq Size: 581
• RefSeq ORF: 393
• Locus ID: 25723
• MW: 13.6 kDa
• Gene Summary: neuropeptide; involved in feeding behavior and hormone secretion regulation [RGD, Feb 2006]