Ndc1 (NM_001025023) Rat Tagged ORF Clone

CAT#: RR215289

Tmem48 (Myc-DDK-tagged ORF) - Rat transmembrane protein 48 (Tmem48), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ndc1
• Synonyms: Tmem48
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215289 representing NM_001025023
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACGGCTGCGAGCGGGCCCTGCGCCGGCGGGTCACCGCGGGACATCCTTTGGCGCGTTTTGGGCT
  GGAGGATAGTTACTAGTGTCGTTTGGTCAGTGCTGCTTCTCCCTGTCTGCATCACAGCATTTATAGTCTT
  CAGCAGCATCGATTTATTCCACCCTATACAGTGGCTGTCAGATTCTCTCAATGAGTTCTATAGTTCCCAA
  GTAATCTTTTACCTCCTGCTGCTGGCCGTGGTCATTATAATAATAAGTATTTTCAATGTGGAGTTCTATA
  CAGTTGTGCCCTCCATACCCGGCTCCCGACTGGCGCTGATAGCACGGATCCTTCACCCTCAGCAGCTCAC
  GCACTCCTTCATACACGCTGTGATGGGAATGGCAGTGGCTTGGTGTGCGGCCATCATGACCAAGGGCCAG
  TCCAGGTCTCTGGTAGTTCCCTGCACTGGAACGGAGAGCTTAGATAGCCCTGCTGCTCAAACCTGCATGA
  ATGAATATCATCTGTTTTTCCTACTGTCTGGAGCATTCATGGGCTATAGCTATAGTCTTCTGTATTTCAT
  TAACAACATGAATTATCTTCCGTTTCCTATTATACAGCAGTACAAATTTTTGCGTTTCCGGAGATCTCTC
  CTCTTACTAGTTAAACATAGCTGTGTGGAATCCCTGTTCATGGTCAGAAATTTCTGCATTGTATATTATT
  TTTTTGGTCATATTCCCAAAGCATGGATTAGCACTGCTCTGGACCTTCGCACAGACGAGCAGGCCCACAG
  GCCTCTTGACACGATAGGCGGTCTCCTGAACGTCTCATTGCTCTACCATGTCTGGCTGTGTGGTGTCTTT
  CTCTTGATGACTTGGTATATCTCATGGATACTCTTCAAAATCTATGCCACAGAGGCTTATATGTTCCCTG
  TTCAGCCACCATTTGCGGAAGAATCAGATGAATGCCTTCCTAAAGTCTTAAATAGCAATCCCCCACTCAT
  CATAAAGTATTTGGCCTTACAGGACCTGATGTTGCTTTCTCAGTATTCTCCTTCTCGAAGGCAAGAAGTT
  TTCAGCCTCAGCCAACCAGGTGGACATCCCCACAATTGGACTGCTATTTCGAGGGAGTGTTTGAGTCTTT
  TAAATGATATGACTCAGAAGCTGGTTCTCTACCAAGAAGCTGCTGCTACGAATGGGCGGATGTATTCCTC
  ATACTCAGTGGAACCCAAGAGACTGAGTTCTCCAGAAGAAACTGCTTTTCAGACTCCAAAACCTAGCCAG
  ATGCCTTCCGTGCCGCCATTAGTTAAAACCTCATTGTTTTCTCCAAAATTAAGTACACCTGACGGTTCAA
  GTCCTTTTGGGACTCCGTTTGGCTCCAGTGTAGTGAACCGAATGGCTGGAATTTTTGATGTAAACACCTG
  CTGTGGATCTCCCCAGAGTCCTCAGCTTGTCAGAAGAGGGCCGAGACTGTGGACTCACACTTCTGATCAG
  CAAGTGTCTGCAGTCTCTAATCCCTCTCCTTGTGCCTCTGTCACTGCTGAGGGCAAGACAGTGAGGCAGC
  CCAGTGTGCTCTACTCGTGGATTCAGAATAAACGGGAGCAGATTAAGAATTTCTTGTCGAAACGGGTGCT
  GATAATGTACTTTTTCAGTAAGCATCCAGAGGCCTCCATTCAGGCTGTTTTTTCAGATGCCCAAATGCAT
  ATTTGGGCTTTAGAAGGTCTGTCTCACTTAGTAGCAGCATCATTTACAGAAGATAGATTTGGAGTTGTCC
  AGACCACGCTCCCAGCTATCCTTAATACTTTGCTGACTCTGCAGGAGGCTGTTGACAAATACTTCAAGCT
  GCCCCATGCTTCTAGTAAACCACCCCGAACTTCAGGAAGCCTCGTGGACACATCCTACAAAACATTAAGA
  TTTGCTTTCAGGGCCTCACTGAAAACTGCTATCTACCGTATAACCACCACGTTTGGTGAACATCTGAATG
  CTGTGCAAGCATCTGCAGAACATCAGAAAAGACTTCAACAGTTCTTGGAGTTCAAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215289 representing NM_001025023
  Red=Cloning site Green=Tags(s)
  
  MATAASGPCAGGSPRDILWRVLGWRIVTSVVWSVLLLPVCITAFIVFSSIDLFHPIQWLSDSLNEFYSSQ
  VIFYLLLLAVVIIIISIFNVEFYTVVPSIPGSRLALIARILHPQQLTHSFIHAVMGMAVAWCAAIMTKGQ
  SRSLVVPCTGTESLDSPAAQTCMNEYHLFFLLSGAFMGYSYSLLYFINNMNYLPFPIIQQYKFLRFRRSL
  LLLVKHSCVESLFMVRNFCIVYYFFGHIPKAWISTALDLRTDEQAHRPLDTIGGLLNVSLLYHVWLCGVF
  LLMTWYISWILFKIYATEAYMFPVQPPFAEESDECLPKVLNSNPPLIIKYLALQDLMLLSQYSPSRRQEV
  FSLSQPGGHPHNWTAISRECLSLLNDMTQKLVLYQEAAATNGRMYSSYSVEPKRLSSPEETAFQTPKPSQ
  MPSVPPLVKTSLFSPKLSTPDGSSPFGTPFGSSVVNRMAGIFDVNTCCGSPQSPQLVRRGPRLWTHTSDQ
  QVSAVSNPSPCASVTAEGKTVRQPSVLYSWIQNKREQIKNFLSKRVLIMYFFSKHPEASIQAVFSDAQMH
  IWALEGLSHLVAASFTEDRFGVVQTTLPAILNTLLTLQEAVDKYFKLPHASSKPPRTSGSLVDTSYKTLR
  FAFRASLKTAIYRITTTFGEHLNAVQASAEHQKRLQQFLEFKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025023
• ORF Size: 2019 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025023.1, NP_001020194.1
• RefSeq Size: 2636 bp
• RefSeq ORF: 2022 bp
• Locus ID: 362557
• Cytogenetics: 5q34
• MW: 75.7 kDa