Polb (NM_017141) Rat Tagged ORF Clone

CAT#: RR215530

Polb (Myc-DDK-tagged ORF) - Rat polymerase (DNA directed), beta (Polb), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Polb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215530 representing NM_017141
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAAACGCAAGGCCCCGCAGGAGACCCTCAACGGCGGCATCACGGACATGCTCGTGGAACTCGCAA
  ACTTTGAGAAGAACGTGAGCCAGGCGATCCACAAGTACAATGCATACAGAAAAGCAGCATCTGTGATAGC
  AAAGTACCCACACAAAATCAAGAGTGGAGCAGAAGCTAAGAAATTGCCAGGAGTAGGAACAAAAATTGCT
  GAAAAGATTGATGAATTTTTAGCAACTGGAAAATTGCGTAAACTGGAAAAGATTCGTCAGGATGATACAA
  GTTCATCCATCAACTTCCTGACTCGAGTTACTGGCATCGGGCCATCTGCTGCAAGGAAGCTTGTAGATGA
  AGGAATTAAAACATTAGAAGATCTCAGGAAAAATGAAGATAAATTGAACCACCATCAGCGAATTGGGCTG
  AAATATTTTGAGGACTTTGAAAAGAGAATTCCTCGTGAGGAGATGCTGCAAATGCAGGACATTGTTCTTA
  ATGAAGTTAAAAAGCTGGATCCCGAGTACATCGCTACAGTCTGCGGCAGTTTCCGAAGAGGCGCAGAGTC
  CAGTGGAGATATGGACGTTCTGCTGACCCACCCAAACTTCACGTCAGAATCAAGCAAACAGCCAAAGTTG
  TTACATCGTGTTGTGGAACAGTTACAAAAAGTCCGTTTCATTACAGATACTCTGTCAAAGGGTGAGACAA
  AGTTCATGGGTGTTTGCCAGCTTCCCAGCGAGAATGATGAAAACGAATATCCACACAGGAGAATCGATAT
  CAGGTTGATCCCCAAAGATCAGTACTACTGTGGTGTTCTCTACTTCACTGGAAGTGACATCTTTAATAAG
  AATATGAGAGCGCATGCCCTGGAAAAGGGCTTCACAATCAATGAGTACACCATCCGCCCCCTGGGGGTCA
  CTGGGGTTGCTGGGGAGCCCCTTCCGGTGGACAGCGAGCAGGACATTTTCGATTACATCCAGTGGCGCTA
  CCGGGAGCCCAAGGACAGGAGTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215530 representing NM_017141
  Red=Cloning site Green=Tags(s)
  
  MSKRKAPQETLNGGITDMLVELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIA
  EKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVTGIGPSAARKLVDEGIKTLEDLRKNEDKLNHHQRIGL
  KYFEDFEKRIPREEMLQMQDIVLNEVKKLDPEYIATVCGSFRRGAESSGDMDVLLTHPNFTSESSKQPKL
  LHRVVEQLQKVRFITDTLSKGETKFMGVCQLPSENDENEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNK
  NMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEQDIFDYIQWRYREPKDRSE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017141
• ORF Size: 1005 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017141.1, NM_017141.2, NP_058837.2
• RefSeq Size: 1196
• RefSeq ORF: 1008
• Locus ID: 29240
• MW: 38.3 kDa
• Gene Summary: plays a role in DNA repair and bypass of pyrimidine dimers produced by UV-irradiation; also involved in base-excision repair [RGD, Feb 2006]