Hfe (NM_001173435) Rat Tagged ORF Clone

CAT#: RR215952

Hfe (myc-DDK-tagged) - Rat hemochromatosis (Hfe), transcript variant 3

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hfe
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215952 representing NM_001173435
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGATCAGCTGGGCTCCCTGTGCGGCTGCTATTGCTGCTGCTGTTGTTGCTGCTGTGGTCCGTGG
  CCCCGCAGGCGCTGCGGCCCGTGCCTACTTTGGTGAAAGTGACTCGCCACTGGGCCTCTACAGGGACCTC
  TCTAAGGTGTCAGGCTCTGAATTTCTTCCCCCAGAACATCACTATGAGGTGGTTGAAGGACAGCCAGCCC
  CTAGATGCCAAGGATGTCAACCCTGAGAACGTGCTGCCAAATGGGGATGGGACCTATCAGGGCTGGCTGA
  CCTTGGCTGTGGCCCCTGGAGAAGAGACAAGGTTCAGCTGTCAAGTGGAGCACCCAGGCCTGGATCAGCC
  TCTCACTGCCACTTGGGAGCCCTCACGGTCTCAGGACATGATTATTGGAATCATAAGTGGGATCACCATT
  TGTGCCATCTTCTTTGTTGGAATTCTGATCCTAGTCTTAAGGAAAAGGAAGGTTTCAGGAGGAACCATGG
  GTGACTATGTCTTAACAGAGTGTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215952 representing NM_001173435
  Red=Cloning site Green=Tags(s)
  
  MDRSAGLPVRLLLLLLLLLLWSVAPQALRPVPTLVKVTRHWASTGTSLRCQALNFFPQNITMRWLKDSQP
  LDAKDVNPENVLPNGDGTYQGWLTLAVAPGEETRFSCQVEHPGLDQPLTATWEPSRSQDMIIGIISGITI
  CAIFFVGILILVLRKRKVSGGTMGDYVLTECE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001173435
• ORF Size: 516 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001173435.1, NP_001166906.1
• RefSeq Size: 1106
• RefSeq ORF: 519
• Locus ID: 29199
• MW: 19 kDa
• Gene Summary: membrane protein; may be involved with iron metabolism; defects in human homolog lead to hereditary hemochromotosis, an iron storage disorder [RGD, Feb 2006]