Bdnf (NM_001270635) Rat Tagged ORF Clone

CAT#: RR216149

Bdnf (myc-DDK-tagged) - Rat brain-derived neurotrophic factor (Bdnf), transcript variant 7

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bdnf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216149 representing NM_001270635
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCATCCTTTTCCTTACTATGGTTATTTCATACTTCGGTTGCATGAAGGCTGCGCCCATGAAAGAAG
  CAAACGTCCACGGACAAGGCAACTTGGCCTACCCAGCTGTGCGGACCCATGGGACTCTGGAGAGCGTGAA
  TGGGCCCAGGGCAGGTTCGAGAGGTCTGACGACGACGTCCCTGGCTGACACTTTTGAGCACGTGATCGAA
  GAGCTGCTGGATGAGGACCAGAAGGTTCGGCCCAACGAAGAAAACCATAAGGACGCGGACTTGTACACTT
  CCCGGGTGATGCTCAGCAGTCAAGTGCCTTTGGAGCCTCCTCTGCTCTTTCTGCTGGAGGAATACAAAAA
  TTACCTGGATGCCGCAAACATGTCTATGAGGGTTCGGCGCCACTCCGACCCCGCCCGCCGTGGGGAGCTG
  AGCGTGTGTGACAGTATTAGCGAGTGGGTCACAGCGGCAGATAAAAAGACTGCAGTGGACATGTCCGGTG
  GGACGGTCACAGTCCTGGAGAAAGTCCCGGTATCAAAAGGCCAACTGAAGCAATATTTCTACGAGACCAA
  GTGTAATCCCATGGGTTACACGAAGGAAGGCTGCAGGGGCATAGACAAAAGGCACTGGAACTCGCAATGC
  CGAACTACCCAATCGTATGTTCGGGCCCTTACTATGGATAGCAAAAAGAGAATTGGCTGGCGGTTCATAA
  GGATAGACACTTCCTGTGTATGTACACTGACCATTAAAAGGGGAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216149 representing NM_001270635
  Red=Cloning site Green=Tags(s)
  
  MTILFLTMVISYFGCMKAAPMKEANVHGQGNLAYPAVRTHGTLESVNGPRAGSRGLTTTSLADTFEHVIE
  ELLDEDQKVRPNEENHKDADLYTSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGEL
  SVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQC
  RTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270635
• ORF Size: 747 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001270635.1, NP_001257564.1
• RefSeq Size: 3896
• RefSeq ORF: 750
• Locus ID: 24225
• MW: 28.1 kDa
• Gene Summary: plays a role in the development of hippocampal long term potentiation; involved in regulation of synaptic plasticity [RGD, Feb 2006]