Acot7 (NM_001146061) Rat Tagged ORF Clone

CAT#: RR216528

Acot7 (myc-DDK-tagged) - Rat acyl-CoA thioesterase 7 (Acot7), transcript variant 1

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acot7
• Synonyms: Bach
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216528 representing NM_001146061
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTGCTTGCTAGGACTCTGTACCTCTGGGAGGTGGGGAGACAGGTAGCCTCTTGGAGCCTGACGT
  CTGGCCAGGAGTGTTTGGTGCTCAGGGAAACATGGTGGGCTTCGATGAGGGCTGTCAGAACCAGAGCAGT
  TCATCACAAGCCTGGACATTGCATCGCCATGGGAAGGATCATGCGTCCGGATGATGCCAACGTGGCCGGC
  AATGTTCACGGAGGGACCATTCTAAAGATGATCGAGGAGGCTGGGGTCATCATCAGCACCCGGCACTGTA
  ACAGCCAGAATGGGGAGCGCTGTGTGGCTGCCCTGGCCCGGGTGGAGCGCACTGACTTCCTGTCGCCCAT
  GTGCATCGGTGAGGTGGCTCACGTCAGCGCAGAGATCACCTATACTTCCAAGCACTCTGTGGAGGTCCAG
  GTCCACGTGTTGTCGGAGAACATCCTCACAGGTACCAAAAAGCTGACCAATAAGGCCACCTTGTGGTATG
  TGCCCCTGTCATTGAAGAATGTGGACAAGGTCCTTGAGGTGCCTCCTATTGTGTATTTACGGCAGGAACA
  GGAGGAGGAGGGTCGGAAACGCTATGAAGCCCAGAAGCTAGAACGCATGGAGACCAAGTGGAGGAACGGA
  GACATTGTCCAGCCCATCCTGAACCCAGAGCCGAACACAGTGAGCTACAGCCAGTCCAGCCTGATCCACC
  TGGTGGGGCCCTCAGACTGCACTCTTCATGGCTTCGTGCACGGAGGTGTCACCATGAAGCTCATGGATGA
  GGTGGCCGGGATTGTGGCTGCGCGCCACTGCAAGACCAATATAGTGACTGCCTCTGTGGATGCTATTAAT
  TTCCATGACAAGATCCGGAAAGGCTGTGTCATCACCATCTCTGGACGCATGACCTTCACAAGCAATAAGT
  CTATGGAAATTGAGGTCCTGGTGGACGCTGACCCTGTGGTGGACAACTCACAGAAGCGCTACCGGGCTGC
  CAGTGCCTTCTTCACCTACGTGTCCCTGAATCAGGAGGGCAAGCCGCTGCCTGTGCCTCAGCTTGTGCCG
  GAGACGGAGGACGAGAAGAAGCGTTTTGAAGAAGGCAAAGGCCGCTATCTGCAGATGAAGGCGAAGCGAC
  AGGGCCATACAGAGCCTCAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216528 representing NM_001146061
  Red=Cloning site Green=Tags(s)
  
  MKLLARTLYLWEVGRQVASWSLTSGQECLVLRETWWASMRAVRTRAVHHKPGHCIAMGRIMRPDDANVAG
  NVHGGTILKMIEEAGVIISTRHCNSQNGERCVAALARVERTDFLSPMCIGEVAHVSAEITYTSKHSVEVQ
  VHVLSENILTGTKKLTNKATLWYVPLSLKNVDKVLEVPPIVYLRQEQEEEGRKRYEAQKLERMETKWRNG
  DIVQPILNPEPNTVSYSQSSLIHLVGPSDCTLHGFVHGGVTMKLMDEVAGIVAARHCKTNIVTASVDAIN
  FHDKIRKGCVITISGRMTFTSNKSMEIEVLVDADPVVDNSQKRYRAASAFFTYVSLNQEGKPLPVPQLVP
  ETEDEKKRFEEGKGRYLQMKAKRQGHTEPQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146061
• ORF Size: 1143 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001146061.1, NP_001139533.1
• RefSeq Size: 1525
• RefSeq ORF: 1146
• Locus ID: 26759
• MW: 42.7 kDa
• Gene Summary: cleaves acyl-CoA thioesters to free fatty acids and coenzyme A [RGD, Feb 2006]