Mapk8 (NM_053829) Rat Tagged ORF Clone

CAT#: RR216642

Mapk8 (myc-DDK-tagged) - Rat mitogen-activated protein kinase 8 (Mapk8)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mapk8
• Synonyms: JNK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216642 representing NM_053829
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGAAGTAAACGTGACAACAATTTTTATAGTGTAGAGATCGGAGATTCTACATTCACAGTCCTAA
  AACGATACCAGAACTTAAAGCCTATAGGCTCAGGAGCTCAAGGAATAGTGTGTGCAGCTTATGATGCTAT
  TCTTGAAAGAAATGTTGCAATCAAGAAGCTCAGCCGGCCATTTCAGAATCAGACCCATGCTAAGCGAGCC
  TACCGAGAACTAGTTCTTATGAAGTGTGTTAATCACAAAAATATAATTGGCCTTTTGAATGTTTTCACAC
  CACAGAAATCCCTAGAAGAATTTCAAGATGTTTACATAGTCATGGAGCTCATGGATGCAAATCTTTGCCA
  AGTGATTCAGATGGAGTTAGATCATGAAAGAATGTCCTACCTTCTCTATCAAATGCTGTGTGGAATCAAG
  CACCTTCACTCTGCTGGAATTATTCATCGGGACTTAAAGCCTAGTAATATAGTAGTCAAATCAGACTGCA
  CTTTGAAGATTCTTGATTTTGGACTGGCAAGGACTGCAGGAACGAGTTTTATGATGACGCCTTACGTGGT
  AACTCGTTACTACAGAGCACCAGAGGTCATTCTCGGCATGGGCTACAAGGAGAACGTGGATTTATGGTCT
  GTGGGGTGCATTATGGGAGAAATGGTTTGCCTCAAAATCCTCTTTCCAGGAAGGGACTATATTGATCAGT
  GGAATAAAGTTATTGAACAGCTCGGAACACCTTGTCCTGAATTCATGAAGAAACTACAACCAACAGTAAG
  GACTTACGTTGAAAACAGACCTAAGTACGCTGGCTATAGCTTTGAGAAACTGTTTCCTGATGTGCTTTTC
  CCAGCTGACTCAGAACATAACAAACTTAAAGCCAGTCAGGCGAGAGATTTGTTATCTAAAATGCTGGTGA
  TAGATGCGTCCAAAAGGATCTCCGTAGACGAAGCTCTCCAGCACCCGTACATCAACGTCTGGTATGATCC
  TTCAGAAGCAGAGGCCCCACCACCAAAGATCCCTGACAAGCAGTTAGATGAAAGGGAGCACACAATAGAG
  GAGTGGAAAGAACTGATATACAAGGAGGTCATGGATTTGGAGGAGCGAACTAAGAATGGCGTCATAAGAG
  GGCAGCCGTCTCCTTTAGGTGCAGCAGTGATCAATGGCTCTCAGCATCCGGTCTCTTCGCCGTCTGTCAA
  TGACATGTCTTCAATGTCCACAGATCCGACTCTGGCCTCGGATACAGACAGCAGTCTAGAAGCATCAGCT
  GGACCTCTGGGCTGCTGTAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216642 representing NM_053829
  Red=Cloning site Green=Tags(s)
  
  MSRSKRDNNFYSVEIGDSTFTVLKRYQNLKPIGSGAQGIVCAAYDAILERNVAIKKLSRPFQNQTHAKRA
  YRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYIVMELMDANLCQVIQMELDHERMSYLLYQMLCGIK
  HLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDLWS
  VGCIMGEMVCLKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLF
  PADSEHNKLKASQARDLLSKMLVIDASKRISVDEALQHPYINVWYDPSEAEAPPPKIPDKQLDEREHTIE
  EWKELIYKEVMDLEERTKNGVIRGQPSPLGAAVINGSQHPVSSPSVNDMSSMSTDPTLASDTDSSLEASA
  GPLGCCR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053829
• ORF Size: 1281 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053829.1, NM_053829.2, NP_446281.2
• RefSeq Size: 5796
• RefSeq ORF: 1284
• Locus ID: 116554
• MW: 48.3 kDa
• Gene Summary: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. [provided by RefSeq, Jul 2012]