Pfkfb1 (NM_001271064) Rat Tagged ORF Clone

CAT#: RR216645

Pfkfb1 (myc-DDK-tagged) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant 3

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pfkfb1
• Synonyms: Pfkfb01; PFRX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216645 representing NM_001271064
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACCCACCGGACCTGCTTTGGGGGTCTGTAAATGCAAGAGAGCCATTGGAAAATTAGCGATGGAAG
  AAAAAGCCTCTAAGAGAACAGTGTTTAATTTAGGTCAGTATCGACGAGAGGCAGTGAGTTACAGGAACTA
  TGAATTCTTTCGCCCAGACAACACAGAGGCCCAGCTTATCAGGAAGCAGTGTGCTCTAGCAGCCCTAAAG
  GATGTCCATAAGTATCTCAGCCGCGAGGAAGGTCATGTTGCGGTTTTTGATGCCACCAACACTACCAGAG
  AACGACGATCGTTGATTCTACAGTTTGCTAAGGAACATGGTTATAAGGTCTTCTTTATTGAGTCTATTTG
  TAATGACCCCGAAATCATTGCAGAAAACATCAAGCAAGTGAAACTTGGTAGTCCTGATTACATAGACTGT
  GACCAAGAAAAGGTTTTGGAAGACTTTCTAAAGAGAATAGAGTGCTATGAGATCAACTACCAACCTTTGG
  ATGAGGAATTGGACAGCCACCTGTCCTACATCAAGATCTTCGACGTGGGCACACGCTACATGGTAAATCG
  AGTGCAGGACCACGTTCAGAGCCGTACAGCCTACTACCTCATGAACATCCATGTCACACCTCGATCTATC
  TACCTATGCCGCCATGGTGAGAGTGAACTCAACCTTAGAGGCCGCATTGGAGGTGACTCTGGCCTCTCAG
  CTCGGGGCAAGCAGTATGCCTATGCACTAGCCAACTTCATCCGGTCTCAAGGCATCAGCTCCCTGAAAGT
  ATGGACTAGCCACATGAAGAGGACCATTCAGACCGCTGAAGCCCTAGGTGTCCCCTATGAACAGTGGAAG
  GCCCTGAATGAGATTGATGCGGGTGTCTGTGAAGAGATGACCTATGAAGAAATTCAGGAACACTACCCTG
  AGGAATTTGCACTACGGGACCAGGATAAATATCGTTACCGCTATCCCAAGGGAGAGTCCTATGAGGATCT
  GGTTCAGCGTCTTGAACCAGTTATAATGGAGCTAGAACGGCAAGAAAATGTACTGGTGATCTGTCACCAG
  GCTGTCATGCGGTGCCTCCTGGCATACTTCCTGGATAAAAGTTCAGATGAGCTGCCCTATCTCAAGTGTC
  CTCTGCATACTGTGCTCAAACTCACACCTGTGGCTTATGGCTGCAGAGTGGAGTCCATCTACCTGAATGT
  GGAGGCTGTGAACACACACCGGGACAAGCCTGAGAATGTGGACATCACCCGTGAAGCTGAGGAAGCCTTG
  GACACTGTACCTGCCCATTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216645 representing NM_001271064
  Red=Cloning site Green=Tags(s)
  
  MPPTGPALGVCKCKRAIGKLAMEEKASKRTVFNLGQYRREAVSYRNYEFFRPDNTEAQLIRKQCALAALK
  DVHKYLSREEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPEIIAENIKQVKLGSPDYIDC
  DQEKVLEDFLKRIECYEINYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHVQSRTAYYLMNIHVTPRSI
  YLCRHGESELNLRGRIGGDSGLSARGKQYAYALANFIRSQGISSLKVWTSHMKRTIQTAEALGVPYEQWK
  ALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQ
  AVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCRVESIYLNVEAVNTHRDKPENVDITREAEEAL
  DTVPAHY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271064
• ORF Size: 1281 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001271064.1, NP_001257993.1
• RefSeq Size: 1581
• RefSeq ORF: 1284
• Locus ID: 24638
• MW: 49.5 kDa
• Gene Summary: bifunctional enzyme; catalyzes synthesis and degradation of fructose 2,6-bisphosphate, a ubiquitous stimulator of glycolysis [RGD, Feb 2006]