Pfkfb1 (NM_001271064) Rat Untagged Clone

CAT#: RN216645

Pfkfb1 (untagged) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant 3

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pfkfb1
• Synonyms: Pfkfb01; PFRX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216645 representing NM_001271064
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACCCACCGGACCTGCTTTGGGGGTCTGTAAATGCAAGAGAGCCATTGGAAAATTAGCGATGGAAG
  AAAAAGCCTCTAAGAGAACAGTGTTTAATTTAGGTCAGTATCGACGAGAGGCAGTGAGTTACAGGAACTA
  TGAATTCTTTCGCCCAGACAACACAGAGGCCCAGCTTATCAGGAAGCAGTGTGCTCTAGCAGCCCTAAAG
  GATGTCCATAAGTATCTCAGCCGCGAGGAAGGTCATGTTGCGGTTTTTGATGCCACCAACACTACCAGAG
  AACGACGATCGTTGATTCTACAGTTTGCTAAGGAACATGGTTATAAGGTCTTCTTTATTGAGTCTATTTG
  TAATGACCCCGAAATCATTGCAGAAAACATCAAGCAAGTGAAACTTGGTAGTCCTGATTACATAGACTGT
  GACCAAGAAAAGGTTTTGGAAGACTTTCTAAAGAGAATAGAGTGCTATGAGATCAACTACCAACCTTTGG
  ATGAGGAATTGGACAGCCACCTGTCCTACATCAAGATCTTCGACGTGGGCACACGCTACATGGTAAATCG
  AGTGCAGGACCACGTTCAGAGCCGTACAGCCTACTACCTCATGAACATCCATGTCACACCTCGATCTATC
  TACCTATGCCGCCATGGTGAGAGTGAACTCAACCTTAGAGGCCGCATTGGAGGTGACTCTGGCCTCTCAG
  CTCGGGGCAAGCAGTATGCCTATGCACTAGCCAACTTCATCCGGTCTCAAGGCATCAGCTCCCTGAAAGT
  ATGGACTAGCCACATGAAGAGGACCATTCAGACCGCTGAAGCCCTAGGTGTCCCCTATGAACAGTGGAAG
  GCCCTGAATGAGATTGATGCGGGTGTCTGTGAAGAGATGACCTATGAAGAAATTCAGGAACACTACCCTG
  AGGAATTTGCACTACGGGACCAGGATAAATATCGTTACCGCTATCCCAAGGGAGAGTCCTATGAGGATCT
  GGTTCAGCGTCTTGAACCAGTTATAATGGAGCTAGAACGGCAAGAAAATGTACTGGTGATCTGTCACCAG
  GCTGTCATGCGGTGCCTCCTGGCATACTTCCTGGATAAAAGTTCAGATGAGCTGCCCTATCTCAAGTGTC
  CTCTGCATACTGTGCTCAAACTCACACCTGTGGCTTATGGCTGCAGAGTGGAGTCCATCTACCTGAATGT
  GGAGGCTGTGAACACACACCGGGACAAGCCTGAGAATGTGGACATCACCCGTGAAGCTGAGGAAGCCTTG
  GACACTGTACCTGCCCATTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271064
• ORF Size: 1284 bp
• Insert Size: 1284
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001271064.1, NP_001257993.1
• RefSeq Size: 1581
• RefSeq ORF: 1284
• Locus ID: 24638
• Gene Summary: bifunctional enzyme; catalyzes synthesis and degradation of fructose 2,6-bisphosphate, a ubiquitous stimulator of glycolysis [RGD, Feb 2006]
  Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.