Slc1a3 (NM_001289943) Rat Tagged ORF Clone

CAT#: RR216654

Slc1a3 (myc-DDK-tagged) - Rat solute carrier family 1 (glial high affinity glutamate transporter), member 3 (Slc1a3), transcript variant Glast1c

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc1a3
• Synonyms: EAAT1; GLAST; GLAST-1; GluT-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216654 representing NM_001289943
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAAAAGCAACGGAGAAGAGCCCAGGATGGGAAGCAGGATGGAAAGATTCCAGCAAGGGGTGCGCA
  AGCGGACGCTCCTGGCCAAGAAGAAAGTTCAGAACATCACCAAGGAGGATGTGAAGAGCTACCTGTTTCG
  GAATGCCTTTGTGCTACTCACCGTCAGCGCTGTCATTGTGGGTACAATCCTTGGATTTGCCCTCCGACCG
  TATAAAATGAGCTACCGGGAGGTCAAGTACTTCTCCTTTCCTGGGGAGCTTCTGATGCGGATGCTGCAGA
  TGTTGGTCTTACCCCTGATCATCTCCAGTCTTGTCACAGGAATGGCGGCCCTAGATAGTAAGGCATCTGG
  GAAGATGGGGATGCGAGCTGTGGTCTATTACATGACTACCACCATCATTGCTGTGGTGATCGGCATAATC
  ATTGTCATCATCATCCACCCCGGAAAGGGCACGAAAGAAAACATGTACAGAGAAGGTAAAATCGTGCAGG
  TGACTGCCGCAGATGCCTTCCTGGATTTAATCAGGTATGCACCTCTGGGCATCCTCTTCTTGATCGCAGG
  GAAGATTGTTGAGATGGAAGATATGGGTGTGATTGGGGGGCAGCTTGCCATGTATACAGTGACAGTCATC
  GTCGGCCTCCTCATTCATGCCGTCATCGTCCTGCCTCTCCTCTACTTCCTGGTAACCCGGAAGAACCCCT
  GGGTTTTCATTGGAGGGTTGCTGCAAGCACTCATCACAGCCCTGGGGACCTCCTCAAGTTCTGCCACCCT
  GCCCATCACTTTCAAGTGCCTGGAAGAAAACAATGGTGTGGACAAACGCATCACCAGATTTGTGCTTCCC
  GTGGGGGCCACCATTAACATGGATGGGACCGCCCTCTACGAGGCTTTGGCCGCCATTTTCATCGCTCAAG
  TTAACAACTTTGACCTGAATTTTGGACAGATTATAACAATAAGTATCACAGCCACAGCCGCAAGCATTGG
  GGCAGCTGGCATTCCTCAGGCCGGTCTAGTCACCATGGTCATCGTGCTGACATCTGTGGGCCTGCCCACG
  GATGACATCACACTCATCATTGCAGTGGACTGGTTTCTGGACCGCCTCCGAACCACCACCAACGTGCTGG
  GTGACTCCCTCGGGGCCGGGATTGTTGAACACTTGTCCCGACATGAACTGAAGAACCGAGATGTTGAAAT
  GGGGAACTCCGTGATTGAGGAGAATGAAATGAAAAAGCCGTATCAGCTGATTGCCCAGGACAATGAACCA
  GAGAAACCCGTGGCAGACAGCGAAACCAAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216654 representing NM_001289943
  Red=Cloning site Green=Tags(s)
  
  MTKSNGEEPRMGSRMERFQQGVRKRTLLAKKKVQNITKEDVKSYLFRNAFVLLTVSAVIVGTILGFALRP
  YKMSYREVKYFSFPGELLMRMLQMLVLPLIISSLVTGMAALDSKASGKMGMRAVVYYMTTTIIAVVIGII
  IVIIIHPGKGTKENMYREGKIVQVTAADAFLDLIRYAPLGILFLIAGKIVEMEDMGVIGGQLAMYTVTVI
  VGLLIHAVIVLPLLYFLVTRKNPWVFIGGLLQALITALGTSSSSATLPITFKCLEENNGVDKRITRFVLP
  VGATINMDGTALYEALAAIFIAQVNNFDLNFGQIITISITATAASIGAAGIPQAGLVTMVIVLTSVGLPT
  DDITLIIAVDWFLDRLRTTTNVLGDSLGAGIVEHLSRHELKNRDVEMGNSVIEENEMKKPYQLIAQDNEP
  EKPVADSETKM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289943
• ORF Size: 1293 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001289943.1, NP_001276872.1
• RefSeq Size: 3596
• RefSeq ORF: 1296
• Locus ID: 29483
• MW: 47.2 kDa
• Gene Summary: carries out Na+ dependent transport of glutamate and aspartate; may regulate neurotransmitter concentration in the central nervous system [RGD, Feb 2006]