Chit1 (NM_001270846) Rat Tagged ORF Clone

CAT#: RR216729

Chit1 (myc-DDK-tagged) - Rat chitinase 1 (chitotriosidase) (Chit1), transcript variant bChiV_4

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chit1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216729 representing NM_001270846
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGTCCCTGGCCTTGGCAGGTGTGACGACTCTGCTGATGATCCAGTGGGGCTCTGCTGCAAAAC
  TGTTCTGCTACTTCACCAACTGGGCCCAGTACCGGTCCGGGGCAGCTCGATTCCTACCTAGGGATGTGGA
  TCCCAACCTGTGTACCCATGTCATCTATGCCTTTGCTGGACTGAACAACCACCAGGTCAGCACTGTAGAG
  CCCAATGACGAGCTTTTCTACCAAGAGCTGAACAGCCTAAAGAAGAGGAACCCCAAGCTCAAGACCCTGT
  TAGCCGTCGGGGGCTGGAGCTTTGGTACCCAGAAGTTCACAGACATGGTGGCCACAGCCAGCACCCGGCA
  GACCTTTGTCAACTCAGCTCTCTCGTTCCTGCGCACTCATGGTTTTGACGGCCTTGACCTTGACTGGGAA
  TACCCAGGAAGCCGAGGGAGCCCAGCAGTAGACAAAGAGAGATTCACAGCGCTGATACAGGATTTGGCCA
  AAGCCTTCCAGGAGGAAGCCCGGGCCTCAGGGAAAAGTCGCCTCCTTCTGACTGCAGCTGTACCAACTGG
  TCGAGGCCATGTGGATGCTGGTTATGAGGTGGACAAGATTGTTCAGAGTTTGGATTTCATCAACCTTATG
  GCCTACGACTTCCACAGCTCCTGGGACAAGACCACAGGGCACAACAGCCCCCTCTACAAAAGGCAAGGAG
  AGACTGGGAAAGATGCTGAAAAAAATGTGGATGCTGCTGTGACGCTCTGGCTGCAGAAGGGGACCCCTGC
  CAGCAAACTGATGCTTGGCATGCCCGCCTACGGACGCTCCTTCACCTTGGCCTCCTCATCAGACAGTGGA
  GTTGGGGCTCCAGCCACAGGACCTGGGGCCCCAGGCCCCTATACTAAGGAAAAGGGGATACTGGTTTACT
  TTGAGGTCTGCTCCTGGAAGGGAAAACAGAGAATCGAGGACCAGAAGGTGCCCTATGTCTCCCAGGGCAA
  CCAATGGGTGGGATTTGATGACAGGGAAAGCTTCAGAGCCAAGGCTGCCTATGTGAAACAGAAGGGACTG
  GGAGGGGCCATGGTCTGGATTCTGGATGGGGATGACTTCAAAGGTTCCTTCTGCAACGAGGGCCAGTACC
  CTCTCATCCGGACACTACACCAGGAGCTGAGCCTTTCATCCGGGCCTCCAAGAAGCCCAGAACAGGAAGT
  ACCTGGGCCACACCAGCCTTCTGAGCCCGAGCAGGGCCCCAGCCCAGGGCTAGATGACTTCTGCCAAGGC
  AAAGCTGATGGGCTCTACCCCAACCCTACAGAAAAGTCCAGTTTCTACAGCTGTGGAGGGGGGCGACTGT
  TCCAGCACAGCTGCCCCCCAGGCCTGGTGTTTATCGACTCTTGCAAATGCTGTACCTGGGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216729 representing NM_001270846
  Red=Cloning site Green=Tags(s)
  
  MVQSLALAGVTTLLMIQWGSAAKLFCYFTNWAQYRSGAARFLPRDVDPNLCTHVIYAFAGLNNHQVSTVE
  PNDELFYQELNSLKKRNPKLKTLLAVGGWSFGTQKFTDMVATASTRQTFVNSALSFLRTHGFDGLDLDWE
  YPGSRGSPAVDKERFTALIQDLAKAFQEEARASGKSRLLLTAAVPTGRGHVDAGYEVDKIVQSLDFINLM
  AYDFHSSWDKTTGHNSPLYKRQGETGKDAEKNVDAAVTLWLQKGTPASKLMLGMPAYGRSFTLASSSDSG
  VGAPATGPGAPGPYTKEKGILVYFEVCSWKGKQRIEDQKVPYVSQGNQWVGFDDRESFRAKAAYVKQKGL
  GGAMVWILDGDDFKGSFCNEGQYPLIRTLHQELSLSSGPPRSPEQEVPGPHQPSEPEQGPSPGLDDFCQG
  KADGLYPNPTEKSSFYSCGGGRLFQHSCPPGLVFIDSCKCCTWG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270846
• ORF Size: 1392 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001270846.1, NP_001257775.1
• RefSeq Size: 1835
• RefSeq ORF: 1395
• Locus ID: 289032
• MW: 50.8 kDa