Ncoa4 (NM_001034008) Rat Tagged ORF Clone

CAT#: RR217015

Ncoa4 (myc-DDK-tagged) - Rat nuclear receptor coactivator 4 (Ncoa4), transcript variant 2

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ncoa4
• Synonyms: MGC72479
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217015 representing NM_001034008
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATACATCCCTGGAGCAGAGTGGCTGCTGCAGTAGTAGAGAGACACTTCTGAGGTGCAGTGATGCAA
  GGAGGGAGCTGGAGCTTGCGATTGGTGGAGTCCTCCGGGCCGAGCAGCAAATGAAAGATAATCTACGAGA
  GGTGAAAGCTCAGATTCACAGCTGCATTAGTCGCCACCTGGAATGCCTTCGAAGCCGAGAGGTGTGGCTC
  AACGAGCAGGTGGATCTCATCTATCAGCTGAAGGAGGAGACGCTGCAGCAGCAGGCTCAGCAGCTGTACT
  GGCTAATGGGCCAATTCAATTGTCTTATTCATCAACTGGAGATTACCCAGAACAAGGATCTTGCCAATCA
  AGTCGCGCTGTGCTTGGAGAGACTGGGCAGTTTGACCCTTAAGCCTGAGGATTCTACTGTCCTGCTCTTT
  GAAGCAGATGCATCTGCCCTGCGTCAGACCATCACCACGTTTGGGTCCCTCAAGACCATTCAAATTCCTG
  AGCACCTGATGGCTCATCCTAGTTCTTCAAGTGTCAGGCCTTTCCTGGAGAAAAGAGGTTATACCCAGGT
  GCCAGAGCAGAAGTCAGCATCCAGCGGCACAGCTGTTTCTCTCAGTGAATGGCTTCTTGTAAGCAAACCT
  GCCATTGGTCCTCAGGCTCCATACGTACCCAGTACCAACCCACAGGACTGGCTCACCCCAAAACAGACCT
  TGGAGAACAGTCAGACTTCTGCCAGAATCTGCAGCGATGCCTGGGGCAATCTGAAGGGCTTAGAAAACTG
  GCTCCTTAACAGTCATCAACAAGAAATTCCTGGAAAACCAAGTTCCTCAAAATGTAATAGCCATTGTAGC
  ACCAGTTCTTTCTCTACTGAAGATGAAAAGGTTGAAGATGTAGAGGTCCTCGATCAAGACGAGCTGGACC
  TGTCTGATTGGTTGGTGACTCCTCAGGAACCCCGTGATCTGGAGAAGCCTGTGAATGGCCGCTCTGACAC
  CAGTGACAAGTTTAAGCTCTTGTTCCAGGTGTTCAGAGAGCCCTACAATGTGAATGATTGGCTTGTCAAG
  CCTGACTCCTGTACCAACTGTCAGGGTAACCAGCCCAGAGGTGTGGAGATTGAGAACCTGGGCAATCTGA
  AATGCCTGAATGACCACTTGGAGGCTAAAAAGTCATTGCCAGTCCCCGGCGTAATCACCGAGGGCTGGCT
  TGTCCAGAACCATCAGGACACATGTAAAGTAGAGGAAGTCTGCAAAGCCAACGAGCCCTGCACCAGCTTT
  GCAGAGTGTGTGTGTGATGACAGCTGTGAGAAGGAAGCGATGCATAAATGGCTTCTGAAGAAAGAAGGGA
  AGGACAAGAATGGAATGCCTATGGGACCCAAATCTGAGTCTGAGAAACACAGAGAGTCCCTGTCTCTGTG
  GCTTTGCCCTTCTAGAAATGAGCTAACAGAACACGCTAAGACACCCAAGACTACGGCTCCTGCTAGAATT
  GCTGACTCCTTCCACATCATAAGGAATAGCTCCTTGTCAGAGTGGCTTATTGGGCCTACATGCAAAGGAG
  GAGGCCCCAAGGAAGTGCCCAATATGGAGGAGAGAGCTAGCAAAGAAATGCTTAAAAGCCCCATGGCCAC
  TTACTGGCGTCCTTTTAACACTGCTGACTGGGTCTTACCAGGAAAGAAAGTGGGAAACCTTAGTCAGTTC
  TCCTCGGGAGAAGATAAGTGGCTACTTCGAAAGAAGGCACAGGAAGTATTTCTTAATTCACCCCTACAAG
  AAGAACGTAACTTTCGCTCAGATTGTTACGGCCTCCCGGCAGTTTGTGATCTCTTTGCCTGTATGCAGCT
  TAAAGTTGATAAGGACAAATGGCTGTACAGAGCCCCTCTTCAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217015 representing NM_001034008
  Red=Cloning site Green=Tags(s)
  
  MNTSLEQSGCCSSRETLLRCSDARRELELAIGGVLRAEQQMKDNLREVKAQIHSCISRHLECLRSREVWL
  NEQVDLIYQLKEETLQQQAQQLYWLMGQFNCLIHQLEITQNKDLANQVALCLERLGSLTLKPEDSTVLLF
  EADASALRQTITTFGSLKTIQIPEHLMAHPSSSSVRPFLEKRGYTQVPEQKSASSGTAVSLSEWLLVSKP
  AIGPQAPYVPSTNPQDWLTPKQTLENSQTSARICSDAWGNLKGLENWLLNSHQQEIPGKPSSSKCNSHCS
  TSSFSTEDEKVEDVEVLDQDELDLSDWLVTPQEPRDLEKPVNGRSDTSDKFKLLFQVFREPYNVNDWLVK
  PDSCTNCQGNQPRGVEIENLGNLKCLNDHLEAKKSLPVPGVITEGWLVQNHQDTCKVEEVCKANEPCTSF
  AECVCDDSCEKEAMHKWLLKKEGKDKNGMPMGPKSESEKHRESLSLWLCPSRNELTEHAKTPKTTAPARI
  ADSFHIIRNSSLSEWLIGPTCKGGGPKEVPNMEERASKEMLKSPMATYWRPFNTADWVLPGKKVGNLSQF
  SSGEDKWLLRKKAQEVFLNSPLQEERNFRSDCYGLPAVCDLFACMQLKVDKDKWLYRAPLQM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034008
• ORF Size: 1866 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034008.1, NP_001029180.1
• RefSeq Size: 3430 bp
• RefSeq ORF: 1869 bp
• Locus ID: 619385
• Cytogenetics: 16p16
• MW: 70.3 kDa