Nrg1 (NM_001271129) Rat Tagged ORF Clone

CAT#: RR217029

Nrg1 (myc-DDK-tagged) - Rat neuregulin 1 (Nrg1), transcript variant 12

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nrg1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217029 representing NM_001271129
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAGCGCAAAGAAGGCAGAGGCAAGGGGAAGGGCAAGAAGAAGGACCGGGGATCCCGCGGGAAGC
  CCGGGCCCGCCGAGGGCGACCCGAGCCCAGCACTGCCTCCCAGATTGAAAGAAATGAAGAGCCAGGAGTC
  AGCTGCAGGCTCCAAGCTAGTGCTCCGGTGCGAAACCAGCTCCGAGTACTCCTCACTCAGATTCAAATGG
  TTCAAGAATGGGAACGAGCTGAACCGCAAAAATAAACCAGAAAACATCAAGATACAGAAGAAGCCAGGGA
  AGTCAGAGCTTCGAATTAACAAAGCATCCCTGGCTGACTCTGGAGAGTATATGTGCAAAGTGATCAGCAA
  GTTAGGAAATGACAGTGCCTCTGCCAACATCACCATTGTTGAGTCAAACGAGTTCATCACTGGCATGCCA
  GCCTCGACTGAGACAGCCTATGTGTCCTCAGAGTCTCCCATTAGAATCTCAGTTTCAACAGAAGGCGCAA
  ACACTTCTTCATCCACATCGACATCCACGACTGGGACCAGCCATCTCATAAAGTGCGCGGAGAAGGAGAA
  AACTTTCTGTGTGAATGGGGGCGAGTGCTTCACGGTGAAGGACCTGTCAAACCCGTCAAGATACTTGTGC
  AAGTGCCAACCTGGATTCACTGGAGCAAGATGTACTGAGAATGTACCCATGAAAGTCCAAACCCAAGAAA
  AAGCGGAGGAACTCTACCAGAAGAGGGTGCTGACAATTACTGGCATCTGTATCGCCCTGCTGGTGGTCGG
  CATCATGTGTGTGGTGGCCTACTGCAAAACCAAGAAGCAGCGGCAGAAGCTTCATGATCGGCTTCGGCAG
  AGTCTTCGGTCAGAACGGAGCAACCTGGTGAACATAGCGAATGGGCCTCACCACCCAAACCCACCGCCAG
  AGAACGTGCAGCTGGTGAATCAATACGTATCTAAAAACGTCATCTCCAGTGAGCATATTGTTGAGAGAGA
  AGTGGAGACTTCCTTTTCCACCAGTCATTACACTTCCACAGCCCATCACTCCACGACTGTCACCCAGACT
  CCTAGTCACAGCTGGAGTAATGGGCACACGGAGAGCATCATTTCAGAAAGCAACTCCGTAATCATGATGT
  CTTCGGTAGAGAACAGCAGGCACAGCAGTCCCGCCGGGGGCCCACGAGGACGTCTTCATGGCCTGGGAGG
  CCCTCGTGATAACAGCTTCCTCAGGCATGCCAGAGAAACCCCTGACTCCTACAGAGACTCTCCTCATAGC
  GAAAGGTATGTATCAGCCATGACCACCCCGGCTCGTATGTCACCTGTAGATTTCCACACGCCAAGCTCCC
  CTAAATCGCCCCCTTCGGAAATGTCTCCACCCGTGTCCAGCATGACGGTGTCAATGCCCTCTGTGGCAGT
  CAGCCCCTTTGTGGAAGAAGAGAGGCCTCTGCTGCTTGTGACGCCACCAAGGCTACGGGAGAAGAAATAT
  GATCATCACCCCCAGCAACTCAACTCCTTTCATCACAACCCTGCACATCAGAGTACCAGCCTCCCCCCTA
  GCCCACTGAGGATAGTGGAGGATGAGGAGTACGAGACGACCCAGGAGTATGAGTCAGTTCAAGAGCCCGT
  TAAGAAAGTCACCAATAGCCGGCGGGCCAAAAGAACCAAGCCCAATGGCCACATTGCCAATAGGTTGGAA
  ATGGACAGCAACACAAGTTCTGTGAGCAGTAACTCAGAAAGTGAGACAGAAGACGAAAGAGTAGGTGAAG
  ACACACCATTCCTGGGCATACAGAACCCCCTGGCAGCCAGCCTTGAGGTGGCCCCTGCCTTCCGTCTGGC
  TGAGAGCAGGACTAACCCAGCAGGCCGCTTCTCCACACAGGAGGAATTACAGGCCAGGCTGTCTAGTGTA
  ATCGCTAACCAAGACCCTATTGCTGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217029 representing NM_001271129
  Red=Cloning site Green=Tags(s)
  
  MSERKEGRGKGKGKKKDRGSRGKPGPAEGDPSPALPPRLKEMKSQESAAGSKLVLRCETSSEYSSLRFKW
  FKNGNELNRKNKPENIKIQKKPGKSELRINKASLADSGEYMCKVISKLGNDSASANITIVESNEFITGMP
  ASTETAYVSSESPIRISVSTEGANTSSSTSTSTTGTSHLIKCAEKEKTFCVNGGECFTVKDLSNPSRYLC
  KCQPGFTGARCTENVPMKVQTQEKAEELYQKRVLTITGICIALLVVGIMCVVAYCKTKKQRQKLHDRLRQ
  SLRSERSNLVNIANGPHHPNPPPENVQLVNQYVSKNVISSEHIVEREVETSFSTSHYTSTAHHSTTVTQT
  PSHSWSNGHTESIISESNSVIMMSSVENSRHSSPAGGPRGRLHGLGGPRDNSFLRHARETPDSYRDSPHS
  ERYVSAMTTPARMSPVDFHTPSSPKSPPSEMSPPVSSMTVSMPSVAVSPFVEEERPLLLVTPPRLREKKY
  DHHPQQLNSFHHNPAHQSTSLPPSPLRIVEDEEYETTQEYESVQEPVKKVTNSRRAKRTKPNGHIANRLE
  MDSNTSSVSSNSESETEDERVGEDTPFLGIQNPLAASLEVAPAFRLAESRTNPAGRFSTQEELQARLSSV
  IANQDPIAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271129
• ORF Size: 1917 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001271129.1, NP_001258058.1
• RefSeq Size: 3255
• RefSeq ORF: 1920
• Locus ID: 112400
• MW: 70.3 kDa
• Gene Summary: ligand for Erbb3 and Erbb4 receptors; gene produces many different alternative splicing isoforms; involved in neural and organ development [RGD, Feb 2006]