Med25 (NM_001170426) Rat Tagged ORF Clone

CAT#: RR217160

Med25 (myc-DDK-tagged) - Rat mediator complex subunit 25 (Med25)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Med25
• Synonyms: RGD1305137
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217160 representing NM_001170426
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCCCGGATCCGAAGGCCCGGCCCGGGCCGGGGGCCTGGTCGCTGATGTGGTGTTTGTGATCGAGG
  GGACGGCAAACCTGGGGCCGTACTTCGAGGGACTCCGCAAGCACTACCTGCTGCCCGCCATAGAGTACTT
  CAACGGAGGACCACCCGCAGAGACGGACTTTGGGGGAGACTATGGCGGAACCCAGTACAGCCTTGTGGTG
  TTCAACACAGTAGACTGCGCTCCAGAGTCCTATGTACAATGCCATGCTCCTACCAGCAGTGCCTATGAGT
  TTGTCACCTGGCTCGATGGCATCAAGTTCATGGGTGGTGGTGGCGAGAGCTGCAGTCTCATTGCTGAAGG
  TCTGAGCACTGCTCTGCAGCTGTTTGATGACTTCAAGAAGATGCGTGAACAGATCGGCCAGACCCACCGA
  GTCTGTCTCCTCATCTGCAACTCCCCCCCATACCTACTGCCTGCCGTGGAGAGCACCACATACTCAGGCT
  GTACGACGGAGAGCCTGGTGCAGAAGATAGGAGAGCGTGGTATCCACTTCTCCATCGTTTCTCCAAGAAA
  GCTGCCAGCACTTAGGCTTCTGTTTGAGAAGGCGGCTCCTCCTGCCCTACTGGAGCCATTGCAGCCCCCA
  GCAGACGTCAGCCAGGACCCCAGGCACATGGTACTTGTGCGTGGGCTCGTGCTGCCTGTTGGCGGCAGCT
  CAGCCCCAGGCCCTCTGCAGCCCAAGCAAGCCGTTCCCCTGCCACCAGCTCCAACTTCAGCTGCCACGCT
  CTCAGCAGCCCCCCAGCAGACCCTGCCCCCGGTCCCACCACAGTACCAGGTCCCTGGGAACCTAAGTGCT
  GCTCAGGTGGCTGCGCAGAATGCTGTGGAGGCTGCCAAGAACCAGAAGGCCGGGCTGGGCCCACGCTTCT
  CACCCATCAACCCTCTCCAGCAAGCTGCTTCAGGAGTGGGGCCCCCCTTCAGCCAGGCCCCGGCACCCCC
  ATTAGCTCCTGGGCCCCCTGGAGCTCCCAAGCCACCTCCTGCCTCACAGCCTAGCCTAGTCTCCACTGTG
  GCCCCTGGACCAGGCCTGGCAGCCCCGGCTCAGCCTGGGGCTCCGTCTATGCAGGCAGGCACTGTGACCC
  CAGGTGGGGTCAGTGGTCCTTCACCAGCCCAGCTGGGGGGTCCTGCACTTGGTGGGCAACAGTCAGTATC
  CAACAAGCTCCTAGCCTGGAGTGGCGTCCTTGAGTGGCAGGAGAAACCCAAGCCTGCATCCGTGGATGCC
  AACACCAAGCTGACAAGGTCTCTGCCTTGTCAGGTCTATGTGAACCATGGAGAAAACCTGAAGACTGAGC
  AATGGCCCCAGAAACTGATCATGCAGCTCATCCCTCAGCAACTGCTGACCACCCTGGGCCCGCTGTTCCG
  GAACTCGAGAATGGTCCAGTTCCACTTCACCAACAAGGACCTGGAGTCCCTCAAAGGCCTCTACCGCATC
  ATGGGCAATGGCTTTGCCGGCTGCGTGCACTTCCCACATACAGCCCCGTGCGAGGTGCGCGTGCTCATGC
  TCCTGTACTCGTCTAAGAAGAAGATCTTCATGGGCCTCATCCCCTACGACCAGAGTGGCTTCGTCAATGG
  CATCCGCCAGGTCATCACCAACCATAAGCAGGTCCAGCAGCAGAAGCTAGAGCAGCAGCGTGGGATGGGG
  GCACAGCAGGCACCTCCAGGCCTGGGCCCCATTCTGGAGGACCAAGCGAGGCCCCCGCAGAATCTATTAC
  AGCTCCGTGCACCACAGCCTCAGCCTCAGGGTGCTGTGGGAGCTTCTGCGGCTACAGGGCAGCCCCAGCC
  CCAAGGTACTACCCAGGCCCCAACAGGGGCACCCCAAGGTCCTCCTGGAGCAGCCCCTGGCCCACCTCCT
  TCTGGACCCATCCTTCGACCCCAGAACCCTGGAGCCAACCCCCAGCTGCGGAGCCTTCTCCTTAATCCAG
  CACCGCCCCAGACTGGGGTACCCCCACCCCAGGCCTCCCTACACCACCTCCAGCCTCCAGGGGCCCCTGC
  ATTGCTGCCGCCACATCAGAGCCTGGGGCAGCCGCAGCTAGGGCCGCAACTCCTGCACCCTCCACCTACC
  CAGTCTTGGCCCACACAGCTACCCCAGAGGGCGCCACTGCCAGGTCAGATGCTGCTGAGCGGGGGTCCCC
  GGGGCCCGGTTCCCCAGCCGGGCCTGCAGCCCAGCGTCATGGAGGACGACATCCTCATGGACCTCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217160 representing NM_001170426
  Red=Cloning site Green=Tags(s)
  
  MVPGSEGPARAGGLVADVVFVIEGTANLGPYFEGLRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVV
  FNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHR
  VCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQPP
  ADVSQDPRHMVLVRGLVLPVGGSSAPGPLQPKQAVPLPPAPTSAATLSAAPQQTLPPVPPQYQVPGNLSA
  AQVAAQNAVEAAKNQKAGLGPRFSPINPLQQAASGVGPPFSQAPAPPLAPGPPGAPKPPPASQPSLVSTV
  APGPGLAAPAQPGAPSMQAGTVTPGGVSGPSPAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDA
  NTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRI
  MGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMG
  AQQAPPGLGPILEDQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGTTQAPTGAPQGPPGAAPGPPP
  SGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPALLPPHQSLGQPQLGPQLLHPPPT
  QSWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170426
• ORF Size: 2238 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001170426.1, NP_001163897.1
• RefSeq Size: 2327
• RefSeq ORF: 2241
• Locus ID: 292889
• MW: 78.1 kDa