Gria2 (NM_001083811) Rat Tagged ORF Clone

CAT#: RR217297

Gria2 (myc-DDK-tagged) - Rat glutamate receptor, ionotropic, AMPA 2 (Gria2), transcript variant 2

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gria2
• Synonyms: GluA2; gluR-B; GluR-K2; GluR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217297 representing NM_001083811
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAAAGATTATGCATATTTCTGTCCTCCTTTCTCCTGTTTTATGGGGACTGATTTTTGGTGTCTCTT
  CTAACAGCATACAGATAGGGGGGCTATTTCCAAGGGGCGCTGATCAAGAATACAGTGCATTTCGGGTAGG
  GATGGTTCAGTTTTCCACTTCGGAGTTCAGACTGACACCCCATATCGACAATTTGGAGGTAGCCAACAGT
  TTCGCAGTCACCAATGCTTTCTGCTCCCAGTTTTCAAGAGGAGTCTACGCAATTTTTGGATTTTATGACA
  AGAAGTCTGTAAATACCATCACATCATTCTGTGGGACACTCCATGTGTCCTTCATCACACCTAGCTTCCC
  AACAGATGGCACACATCCATTTGTCATCCAGATGCGACCTGACCTCAAAGGAGCACTCCTTAGCTTGATT
  GAGTACTACCAATGGGACAAGTTCGCATACCTCTATGACAGTGACAGAGGCTTATCAACACTGCAAGCTG
  TTCTGGATTCTGCTGCAGAGAAGAAGTGGCAGGTGACTGCTATCAATGTGGGGAACATCAACAATGACAA
  GAAAGATGAGACCTACAGATCGCTCTTTCAAGATCTGGAGTTAAAAAAAGAACGGCGTGTAATCCTGGAC
  TGTGAAAGGGATAAAGTAAATGACATTGTGGACCAGGTTATTACCATTGGAAAACATGTTAAAGGGTACC
  ATTATATCATTGCAAATCTGGGATTCACTGATGGGGACCTGCTGAAAATTCAGTTTGGAGGAGCAAATGT
  CTCTGGATTTCAGATTGTAGACTACGACGATTCCCTGGTGTCTAAATTTATAGAAAGATGGTCAACACTG
  GAAGAGAAAGAATACCCTGGAGCACACACAGCGACAATTAAGTATACTTCGGCCCTGACGTATGATGCTG
  TCCAAGTGATGACTGAAGCATTCCGTAACCTTCGGAAGCAGAGGATTGAAATATCCCGGAGAGGAAATGC
  AGGAGATTGTTTGGCCAACCCAGCTGTGCCCTGGGGACAAGGGGTCGAAATAGAAAGGGCCCTCAAGCAG
  GTTCAAGTTGAAGGCCTCTCTGGAAATATAAAGTTTGACCAGAATGGAAAACGAATAAACTACACAATTA
  ACATCATGGAGCTCAAAACAAATGGACCCCGGAAGATTGGGTACTGGAGTGAAGTGGATAAAATGGTTGT
  CACCCTAACTGAGCTCCCATCAGGAAATGACACGTCTGGGCTTGAAAACAAGACTGTGGTGGTCACCACA
  ATATTGGAATCTCCATATGTTATGATGAAGAAAAATCATGAAATGCTTGAAGGGAATGAGCGTTACGAGG
  GCTACTGTGTTGACTTAGCTGCAGAAATTGCTAAACACTGTGGGTTCAAGTACAAGCTGACTATTGTTGG
  GGATGGCAAGTATGGGGCCAGGGATGCCGACACCAAAATTTGGAATGGTATGGTTGGAGAGCTTGTCTAC
  GGGAAAGCTGACATTGCAATTGCTCCATTAACTATCACTCTCGTGAGAGAAGAGGTGATTGACTTCTCCA
  AGCCCTTCATGAGTCTTGGAATCTCTATCATGATCAAGAAGCCTCAGAAGTCCAAACCAGGAGTGTTTTC
  CTTTCTTGATCCTTTAGCCTATGAGATCTGGATGTGCATTGTGTTTGCCTACATTGGGGTCAGTGTAGTT
  TTATTCCTGGTCAGCAGATTTAGCCCCTACGAGTGGCACACTGAGGAATTTGAAGATGGAAGAGAAACAC
  AAAGTAGTGAATCAACTAATGAATTTGGGATTTTTAATAGTCTCTGGTTTTCCTTGGGTGCCTTTATGCG
  GCAAGGATGCGATATTTCGCCAAGATCCCTCTCTGGGCGCATTGTTGGAGGTGTGTGGTGGTTCTTTACC
  CTGATCATAATCTCCTCCTACACGGCTAACTTAGCTGCCTTCCTGACTGTAGAGAGGATGGTGTCTCCCA
  TCGAAAGTGCTGAGGATCTGTCTAAGCAAACAGAAATTGCTTATGGAACATTAGACTCTGGCTCCACTAA
  AGAGTTTTTCAGGAGATCTAAAATCGCAGTGTTTGATAAAATGTGGACTTATATGAGGAGTGCAGAGCCC
  TCTGTGTTTGTGAGGACTACCGCAGAAGGAGTAGCCAGAGTCCGGAAATCCAAAGGAAAGTATGCCTACT
  TGCTGGAGTCCACAATGAACGAGTACATCGAGCAGAGGAAGCCTTGTGACACCATGAAAGTGGGAGGAAA
  CTTGGATTCCAAAGGCTACGGCATCGCCACACCTAAAGGATCCTCATTAGGAAATGCGGTTAACCTCGCA
  GTACTAAAACTGAATGAACAAGGCCTGTTGGACAAATTGAAAAACAAATGGTGGTACGACAAAGGAGAGT
  GCGGCAGCGGGGGAGGTGATTCCAAGGAAAAGACCAGTGCCCTCAGTCTGAGCAACGTTGCTGGAGTATT
  CTACATCCTTGTCGGGGGCCTTGGTTTGGCAATGCTGGTGGCTTTGATTGAGTTCTGTTACAAGTCAAGG
  GCCGAGGCGAAACGAATGAAGGTGGCAAAGAATCCACAGAATATTAACCCATCTTCCTCGCAGAATTCCC
  AGAATTTTGCAACTTATAAGGAAGGTTACAACGTATATGGCATCGAGAGTGTTAAAATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217297 representing NM_001083811
  Red=Cloning site Green=Tags(s)
  
  MQKIMHISVLLSPVLWGLIFGVSSNSIQIGGLFPRGADQEYSAFRVGMVQFSTSEFRLTPHIDNLEVANS
  FAVTNAFCSQFSRGVYAIFGFYDKKSVNTITSFCGTLHVSFITPSFPTDGTHPFVIQMRPDLKGALLSLI
  EYYQWDKFAYLYDSDRGLSTLQAVLDSAAEKKWQVTAINVGNINNDKKDETYRSLFQDLELKKERRVILD
  CERDKVNDIVDQVITIGKHVKGYHYIIANLGFTDGDLLKIQFGGANVSGFQIVDYDDSLVSKFIERWSTL
  EEKEYPGAHTATIKYTSALTYDAVQVMTEAFRNLRKQRIEISRRGNAGDCLANPAVPWGQGVEIERALKQ
  VQVEGLSGNIKFDQNGKRINYTINIMELKTNGPRKIGYWSEVDKMVVTLTELPSGNDTSGLENKTVVVTT
  ILESPYVMMKKNHEMLEGNERYEGYCVDLAAEIAKHCGFKYKLTIVGDGKYGARDADTKIWNGMVGELVY
  GKADIAIAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVV
  LFLVSRFSPYEWHTEEFEDGRETQSSESTNEFGIFNSLWFSLGAFMRQGCDISPRSLSGRIVGGVWWFFT
  LIIISSYTANLAAFLTVERMVSPIESAEDLSKQTEIAYGTLDSGSTKEFFRRSKIAVFDKMWTYMRSAEP
  SVFVRTTAEGVARVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSSLGNAVNLA
  VLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKEKTSALSLSNVAGVFYILVGGLGLAMLVALIEFCYKSR
  AEAKRMKVAKNPQNINPSSSQNSQNFATYKEGYNVYGIESVKI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001083811
• ORF Size: 2649 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001083811.1, NP_001077280.1
• RefSeq Size: 6745
• RefSeq ORF: 2652
• Locus ID: 29627
• MW: 98.7 kDa
• Gene Summary: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene. [provided by RefSeq, Jul 2008]