Atp12a (NM_001301664) Rat Tagged ORF Clone

CAT#: RR217338

Atp12a (myc-DDK-tagged) - Rat ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a), transcript variant 2

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp12a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217338 representing NM_001301664
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGGGGGCTTTTCCATCCTCCTGTGGATAGGTGCTGCCTTGTGCTGGATCGCATTTGTGATCCAGT
  ACGTCAACAATTCCGCATCCCTAGACAACGTCTACTTGGGCGCCATACTTGTCCTGGTTGTTATTTTAAC
  GGGGATTTTTGCTTATTACCAAGAAGCCAAAAGCACCAACATCATGGCCAGCTTTAGTAAGATGATCCCC
  CAGCAAGCTCTTGTCATCCGAGATGCCGAAAAGAAGGTCATTTCAGCAGAGCAACTGGTGGTGGGGGACG
  TTGTGGAGATTAAAGGAGGGGACCAGATCCCTGCAGACATCAGACTGGTATTTTCCCAAGGGTGCAAAGT
  AGATAACTCCTCTCTCACTGGGGAATCTGAGCCCCAGGCTCGTTCAACTGAGTTTACCCATGAAAACCCC
  TTGGAAACCAAGAACATAGGCTTCTATTCCACAACCTGCCTGGAAGGTACAGCAACTGGCATTGTCATCA
  ACACGGGTGACCGTACCATCATCGGGCGCATAGCCTCCTTGGCTTCAGGCGTTGGGAGTGAGAAAACACC
  CATTGCCATTGAGATCGAGCATTTTGTCCATATTGTGGCAGGAGTGGCTGTGTCCATTGGCATCATTTTC
  TTCATCACCGCCGTGTGCATGAAGTACTATGTCCTCGACGCCATCATCTTCCTCATTAGCATCATTGTGG
  CTAACGTGCCAGAGGGCCTCCTGGCTACTGTCACTGTTACCCTGTCACTGACGGCAAAACGGATGGCCAA
  GAAGAACTGCCTGGTAAAGAATCTGGAGGCAGTGGAGACTCTTGGCTCCACCTCCATCATCTGCTCAGAC
  AAGACGGGGACCCTGACCCAGAACAGGATGACTGTGGCTCACTTGTGGTTTGACAATCAGATCTTTGTGG
  CTGATACTAGTGAAAACCAAACAAAACAAGCCTTTGACCAAAGCTCTGGAACCTGGGCTTCCTTGTCCAA
  GATAATAACACTGTGTAACCGAGCAGAGTTCAGGCCAGGCCAGGAGAGTGTCCCCATCATGAAGAGAACT
  GTGGTTGGAGACGCCTCAGAAACTGCTCTGCTAAAGTTCTCAGAGGTCATTTTGGGTGACGTGATGGGAA
  TTAGGAAAAGAAACCACAAAGTGGCTGAAATTCCTTTTAATTCTACCAACAAATTTCAGCTCTCCATACA
  TGAGACAGAGGACCCTAATGACAAACGCTTCCTCGTGGTGATGAAAGGAGCCCCTGAGAGGATCTTAGAG
  AAGTGCAGCACCATCATGATCAATGGCCAGGAGCAGCCACTGGACAAGAGCTCTGCTGATAGCTTCCACA
  CAGCCTACATGGAGCTGGGCGGTCTGGGCGAGCGTGTGTTGGGTTTCTGTCACCTCTACTTGCCAGCAGA
  GCAGTTCCCCCAAAGTTACATCTTTGATGTCGACTCCGTAAACTTTCCCACTTCCAACTTCTGCTTTGTG
  GGGCTTCTGTCCATGATCGACCCTCCTCGGTCTACTGTGCCAGACGCAGTCTCCAAATGTCGGAGTGCCG
  GGATCAAGGTTATCATGGTCACAGGTGATCACCCTATCACCGCCAAAGCTATTGCCAAGAGTGTAGGAAT
  CATATCGGCCAACAACGAGACAGTGGAAGACATCGCGAAACGCCGCAATATCGCTGTGGAGCAGGTCAAC
  AAAAGAGAAGCTAAAGCCGCTGTGGTGACTGGCATGGAGCTGAAGGACATGACCCCAGAACAACTGGATG
  AGCTTTTGACCAACTACCAAGAAATCGTATTTGCCCGGACATCACCCCAGCAGAAGTTGATCATTGTGGA
  GGGCTGTCAGAGGCAGGACGCCATCGTTGCCGTGACGGGTGATGGAGTGAACGACTCTCCAGCACTGAAG
  AAGGCAGACATCGGGATTGCTATGGGGATAGCAGGTTCTGACGCTGCTAAGAACGCAGCTGACATGGTCT
  TACTAGACGACAACTTCGCGTCTATAGTCACAGGGGTGGAGGAAGGTCGCCTGATCTTCGACAACCTGAA
  GAAAACCATTGCGTACACCCTGACAAAGAACATCGCCGAGCTCTGCCCTTTTTTGATCTACATCGTTGCT
  GGGCTCCCCCTGCCTATTGGAACCATCACTATCCTATTCATCGACCTGGGCACAGACATTATCCCCTCCA
  TTGCCTTGGCCTATGAGAAAGCAGAAAGTGACATTATGAATAGGAAACCTCGCCACAAGAAGAAAGACAG
  ACTGGTGAACACGCAGCTGGCCATCTACTCTTACCTTCACATTGGTCTCATGCAAGCTCTGGGAGGTTTC
  CTCGTCTATTTCACTGTCTATGCACAGCAGGGCTTTTGGCCCACTTCTCTCATCAACCTGAGGGTAGCAT
  GGGAAACAGATGACATAAATGACTTGGAAGACAGCTACGGGCAGGAATGGACAAGGTATCAGAGAAAATA
  CCTGGAATGGACAGGCTCAACGGCTTTCTTTGTTGCCATCATGATCCAACAAATTGCGGATCTGATCATC
  AGGAAAACCCGGAGGAATTCCATCTTTCAGCAGGGGCTCTTCAGAAATAAAGTCATCTGGGTGGGGATCG
  CCTCACAGGTCATCGTTGCACTGATCCTCTCCTATGGTCTCGGGAGTGTCCCAGCTCTGAGCTTTACCAT
  GCTCAGGGTTCAGTACTGGTTTGTGGCTGTGCCACATGCCATTTTGATTTGGGTGTATGACGAGATGCGA
  AAGTTGTTCATCAGGCTCTACCCTGGAAGCTGGTGGGATAAGAACATGTATTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217338 representing NM_001301664
  Red=Cloning site Green=Tags(s)
  
  MVGGFSILLWIGAALCWIAFVIQYVNNSASLDNVYLGAILVLVVILTGIFAYYQEAKSTNIMASFSKMIP
  QQALVIRDAEKKVISAEQLVVGDVVEIKGGDQIPADIRLVFSQGCKVDNSSLTGESEPQARSTEFTHENP
  LETKNIGFYSTTCLEGTATGIVINTGDRTIIGRIASLASGVGSEKTPIAIEIEHFVHIVAGVAVSIGIIF
  FITAVCMKYYVLDAIIFLISIIVANVPEGLLATVTVTLSLTAKRMAKKNCLVKNLEAVETLGSTSIICSD
  KTGTLTQNRMTVAHLWFDNQIFVADTSENQTKQAFDQSSGTWASLSKIITLCNRAEFRPGQESVPIMKRT
  VVGDASETALLKFSEVILGDVMGIRKRNHKVAEIPFNSTNKFQLSIHETEDPNDKRFLVVMKGAPERILE
  KCSTIMINGQEQPLDKSSADSFHTAYMELGGLGERVLGFCHLYLPAEQFPQSYIFDVDSVNFPTSNFCFV
  GLLSMIDPPRSTVPDAVSKCRSAGIKVIMVTGDHPITAKAIAKSVGIISANNETVEDIAKRRNIAVEQVN
  KREAKAAVVTGMELKDMTPEQLDELLTNYQEIVFARTSPQQKLIIVEGCQRQDAIVAVTGDGVNDSPALK
  KADIGIAMGIAGSDAAKNAADMVLLDDNFASIVTGVEEGRLIFDNLKKTIAYTLTKNIAELCPFLIYIVA
  GLPLPIGTITILFIDLGTDIIPSIALAYEKAESDIMNRKPRHKKKDRLVNTQLAIYSYLHIGLMQALGGF
  LVYFTVYAQQGFWPTSLINLRVAWETDDINDLEDSYGQEWTRYQRKYLEWTGSTAFFVAIMIQQIADLII
  RKTRRNSIFQQGLFRNKVIWVGIASQVIVALILSYGLGSVPALSFTMLRVQYWFVAVPHAILIWVYDEMR
  KLFIRLYPGSWWDKNMYY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301664
• ORF Size: 2784 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001301664.1, NP_001288593.1
• RefSeq Size: 4160
• RefSeq ORF: 2787
• Locus ID: 171028
• MW: 102.5 kDa
• Gene Summary: may act as a H+,K(+)-ATPase in distal colon; may also play a role in K+ absorption and H+ secretion in the distal nephron [RGD, Feb 2006]