Atp12a (NM_001301664) Rat Untagged Clone

CAT#: RN217338

Atp12a (untagged) - Rat ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a), transcript variant 2

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Atp12a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217338 representing NM_001301664
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGGGGGCTTTTCCATCCTCCTGTGGATAGGTGCTGCCTTGTGCTGGATCGCATTTGTGATCCAGT
  ACGTCAACAATTCCGCATCCCTAGACAACGTCTACTTGGGCGCCATACTTGTCCTGGTTGTTATTTTAAC
  GGGGATTTTTGCTTATTACCAAGAAGCCAAAAGCACCAACATCATGGCCAGCTTTAGTAAGATGATCCCC
  CAGCAAGCTCTTGTCATCCGAGATGCCGAAAAGAAGGTCATTTCAGCAGAGCAACTGGTGGTGGGGGACG
  TTGTGGAGATTAAAGGAGGGGACCAGATCCCTGCAGACATCAGACTGGTATTTTCCCAAGGGTGCAAAGT
  AGATAACTCCTCTCTCACTGGGGAATCTGAGCCCCAGGCTCGTTCAACTGAGTTTACCCATGAAAACCCC
  TTGGAAACCAAGAACATAGGCTTCTATTCCACAACCTGCCTGGAAGGTACAGCAACTGGCATTGTCATCA
  ACACGGGTGACCGTACCATCATCGGGCGCATAGCCTCCTTGGCTTCAGGCGTTGGGAGTGAGAAAACACC
  CATTGCCATTGAGATCGAGCATTTTGTCCATATTGTGGCAGGAGTGGCTGTGTCCATTGGCATCATTTTC
  TTCATCACCGCCGTGTGCATGAAGTACTATGTCCTCGACGCCATCATCTTCCTCATTAGCATCATTGTGG
  CTAACGTGCCAGAGGGCCTCCTGGCTACTGTCACTGTTACCCTGTCACTGACGGCAAAACGGATGGCCAA
  GAAGAACTGCCTGGTAAAGAATCTGGAGGCAGTGGAGACTCTTGGCTCCACCTCCATCATCTGCTCAGAC
  AAGACGGGGACCCTGACCCAGAACAGGATGACTGTGGCTCACTTGTGGTTTGACAATCAGATCTTTGTGG
  CTGATACTAGTGAAAACCAAACAAAACAAGCCTTTGACCAAAGCTCTGGAACCTGGGCTTCCTTGTCCAA
  GATAATAACACTGTGTAACCGAGCAGAGTTCAGGCCAGGCCAGGAGAGTGTCCCCATCATGAAGAGAACT
  GTGGTTGGAGACGCCTCAGAAACTGCTCTGCTAAAGTTCTCAGAGGTCATTTTGGGTGACGTGATGGGAA
  TTAGGAAAAGAAACCACAAAGTGGCTGAAATTCCTTTTAATTCTACCAACAAATTTCAGCTCTCCATACA
  TGAGACAGAGGACCCTAATGACAAACGCTTCCTCGTGGTGATGAAAGGAGCCCCTGAGAGGATCTTAGAG
  AAGTGCAGCACCATCATGATCAATGGCCAGGAGCAGCCACTGGACAAGAGCTCTGCTGATAGCTTCCACA
  CAGCCTACATGGAGCTGGGCGGTCTGGGCGAGCGTGTGTTGGGTTTCTGTCACCTCTACTTGCCAGCAGA
  GCAGTTCCCCCAAAGTTACATCTTTGATGTCGACTCCGTAAACTTTCCCACTTCCAACTTCTGCTTTGTG
  GGGCTTCTGTCCATGATCGACCCTCCTCGGTCTACTGTGCCAGACGCAGTCTCCAAATGTCGGAGTGCCG
  GGATCAAGGTTATCATGGTCACAGGTGATCACCCTATCACCGCCAAAGCTATTGCCAAGAGTGTAGGAAT
  CATATCGGCCAACAACGAGACAGTGGAAGACATCGCGAAACGCCGCAATATCGCTGTGGAGCAGGTCAAC
  AAAAGAGAAGCTAAAGCCGCTGTGGTGACTGGCATGGAGCTGAAGGACATGACCCCAGAACAACTGGATG
  AGCTTTTGACCAACTACCAAGAAATCGTATTTGCCCGGACATCACCCCAGCAGAAGTTGATCATTGTGGA
  GGGCTGTCAGAGGCAGGACGCCATCGTTGCCGTGACGGGTGATGGAGTGAACGACTCTCCAGCACTGAAG
  AAGGCAGACATCGGGATTGCTATGGGGATAGCAGGTTCTGACGCTGCTAAGAACGCAGCTGACATGGTCT
  TACTAGACGACAACTTCGCGTCTATAGTCACAGGGGTGGAGGAAGGTCGCCTGATCTTCGACAACCTGAA
  GAAAACCATTGCGTACACCCTGACAAAGAACATCGCCGAGCTCTGCCCTTTTTTGATCTACATCGTTGCT
  GGGCTCCCCCTGCCTATTGGAACCATCACTATCCTATTCATCGACCTGGGCACAGACATTATCCCCTCCA
  TTGCCTTGGCCTATGAGAAAGCAGAAAGTGACATTATGAATAGGAAACCTCGCCACAAGAAGAAAGACAG
  ACTGGTGAACACGCAGCTGGCCATCTACTCTTACCTTCACATTGGTCTCATGCAAGCTCTGGGAGGTTTC
  CTCGTCTATTTCACTGTCTATGCACAGCAGGGCTTTTGGCCCACTTCTCTCATCAACCTGAGGGTAGCAT
  GGGAAACAGATGACATAAATGACTTGGAAGACAGCTACGGGCAGGAATGGACAAGGTATCAGAGAAAATA
  CCTGGAATGGACAGGCTCAACGGCTTTCTTTGTTGCCATCATGATCCAACAAATTGCGGATCTGATCATC
  AGGAAAACCCGGAGGAATTCCATCTTTCAGCAGGGGCTCTTCAGAAATAAAGTCATCTGGGTGGGGATCG
  CCTCACAGGTCATCGTTGCACTGATCCTCTCCTATGGTCTCGGGAGTGTCCCAGCTCTGAGCTTTACCAT
  GCTCAGGGTTCAGTACTGGTTTGTGGCTGTGCCACATGCCATTTTGATTTGGGTGTATGACGAGATGCGA
  AAGTTGTTCATCAGGCTCTACCCTGGAAGCTGGTGGGATAAGAACATGTATTATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301664
• ORF Size: 2787 bp
• Insert Size: 2787
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001301664.1, NP_001288593.1
• RefSeq Size: 4160
• RefSeq ORF: 2787
• Locus ID: 171028
• Gene Summary: may act as a H+,K(+)-ATPase in distal colon; may also play a role in K+ absorption and H+ secretion in the distal nephron [RGD, Feb 2006]
  Transcript Variant: This variant (2) has an alternate 5' sequence, which results in translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (b, also known as HKalpha2b) has a shorter N-terminus, compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.