Srebf1 (NM_001276707) Rat Tagged ORF Clone

CAT#: RR217493

Srebf1 (myc-DDK-tagged) - Rat sterol regulatory element binding transcription factor 1 (Srebf1), transcript variant 1

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Srebf1
• Synonyms: ADD-1; ADD1; SREBP-1; SREBP-1c; Srebp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217493 representing NM_001276707
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGAGCTACCCTTCGGTGAGGCGGCTCTGGAACAGGCACTGGCCGAGGTGTGCGAAATGGACGCGG
  CGCTCTTGACCGACATCGAAGACATGCTTCAGCTCATCAACAACCAAGACAGTGACTTCCCTGGCCTATT
  TGATGCCCCCTATGCTGGGGGTGAGACAGGAGACACAGGCCCCAGCAGCCCTGGTGCCAGCTCTCCTGAG
  AGCTTCTCTTCTCCTGCTTCTCTGGGCTCCTCTCTGGAAGCCTTCCTGGGAGGACCCAAGGTGACACCTG
  CACCCTTGTCCCCTCCACCATCGGCACCCACTGCTGTAAAGATGTACCCGTCCGTGCCCCCCTTCTCCCC
  TGGGCCTGGAATCAAAGAGGAGCCAGTGCCACTCACCATCCTGCAGCCCCCAGCACCACAGCCATCGCCA
  GGGACCCTGTTGCCTCCGAGCTTCCCTCCTCCACCTGTGCAGCTCAGCCCTGCTCCTGTGCTGGGGTACT
  CAAGCCTGCCTTCCGGCTTCTCAGGAACCCTTCCTGGGAACACCCAGCAGACGCCATCTAGCCTGCCACT
  GGGCTCCACGCCAGGAATCTCGCCCACCCCCTTACACACCCAGGTCCAGAGCTCGGCCGCCCAGCAGCCG
  CCGCCAGCCTCAGCAGCCCCTAGAATGAGCACTGTGGCCTCACAGATCCAGCAGGTCCCCGTTGTACTGC
  AGCCACACTTCATCAAGGCAGACTCGCTGCTGCTGACAGCTGTAAAGACAGACACAGGAGCCACAATGAA
  GACCGCAGGCATCAACACCCTGGCTCCTGGCACAGCCGTGCAGGCAGGCCCCTTGCAGACCCTGGTGAGT
  GGAGGGACCATCCTGGCCACAGTACCACTGGTTGTGGACACAGACAAACTGCCCATCCACCGACTAGCAG
  CTGGTGGCAAGGCCCTGGGCTCAGCTCAGAGCCGTGGTGAGAAGCGCACAGCCCACAATGCCATTGAGAA
  GCGCTACCGTTCCTCTATCAATGACAAGATTGTGGAGCTCAAGGACCTGGTGGTGGGCACTGAGGCAAAG
  CTGAATAAATCTGCTGTCTTGCGCAAGGCCATCGACTACATCCGCTTCTTACAGCACAGCAACCAGAAAC
  TCAAGCAGGAGAACCTGACCCTGCGAAGTGCTCACAAAAGCAAATCACTGAAAGACCTGGTGTCAGCTTG
  TGGCAGTGGAGGAGGCACAGATGTGTCTATGGAGGGCATGAAACCTGAAGTGGTAGAAACGCTGACCCCT
  CCACCCTCAGACGCCGGCTCACCCTCCCAGAGTAGCCCCTTGTCCTTGGGCAGCAGAGGCAGCAGCAGTG
  GTGGCAGTGACTCTGAGCCCGACAGCCCAGCCTTTGAGGATAACCAGGTGAAAGCCCAGCGGCTGCCTTC
  ACATAGCCGAGGCATGCTGGACCGCTCCCGCCTGGCCCTGTGTGTACTGGTCTTCCTGTGTCTGACCTGC
  AACCCATTGGCCTCACTGTTTGGCTGGGGCATCCTCACTCCCTCTGATGCTTCGGGTGTGCACCGTAGTT
  CTGGGCGCAGCATGCTGGAGGCCGAGAGCAGAGATGGCTCTAATTGGACCCAGTGGTTGCTGCCACCCCT
  AGTCTGGCTGGCCAATGGACTACTAGTGTTGGCCTGCTTGGCTCTTCTCTTTGTCTACGGGGAACCTGTG
  ACCAGGCCACACTCCGGCCCGGCTGTACACTTCTGGAGACATCGCAAACAAGCTGACCTGGATTTGGCCC
  GGGGAGATTTTGCCCAGGCCGCTCAACAGCTGTGGCTGGCCTTGCAAGCCCTGGGCCGGCCCCTGCCCAC
  CTCAAACCTGGATCTGGCCTGCAGCCTGCTTTGGAACCTCGTCCGCCACCTGCTGCAGCGTCTTTGGGTG
  GGCCGCTGGCTGGCAGGCCAGGCTGGGGGCCTGCAGAGGGACTACAGGCTGAGAAAGGATGCTCGTGCCA
  GTGCCCGAGATGCGGCTGTCGTCTACCATAAGCTGCACCAGCTGCATGCCATGGGCAAGTACACAGGAGG
  CCATCTTGTTGCTTCTAACCTGGCACTGAGTGCCCTTAACCTGGCTGAGTGTGCAGGAGATGCTATATCC
  ATGGCAACACTGGCAGAGATCTACGTGGCAGCTGCCCTAAGGGTCAAAACCAGCCTCCCCAGAGCCTTGC
  ACTTCTTGACACGTTTCTTCCTGAGTAGTGCCCGCCAGGCCTGCCTGGCACAGAGTGGTGCAGTGCCTCT
  TGCCATGCAGTGGCTCTGCCACCCTGTAGGTCACCGTTTCTTCGTGGATGGGGACTGGGCTGTACACGGT
  GCCCCCCAGGAGAGTCTGTACAGCGTGGCTGGGAACCCAGTGGATCCACTGGCCCAGGTGACCCGACTAT
  TCTGTGAACATCTCCTGGAGCGAGCATTGAACTGTATCGCTCAGCCCAGCCCAGGGGCAGCTGATGGAGA
  CAGGGAGTTCTCAGATGCTCTTGGATATCTACAGTTGCTAAATAGCTGTTCTGACGCTGTCGGAGCTCCT
  GCGTGCAGCTTCTCTGTCAGTTCCAGCATGGCTACCACCACTGGCACAGACCCAGTGGCCAAGTGGTGGG
  CCTCACTGACAGCCGTGGTGATCCACTGGCTGAGGCGGGATGAGGAGGCAGCTGAACGCTTATACCCACT
  GGTAGAGCACATTCCCCAAGTGCTGCAGGAAACTGAGAGACCCCTTCCCAGGGCAGCTCTGTACTCCTTC
  AAGGCTGCCCGGGCTCTGCTGGACCACAGAAAGGTGGAATCCAGCCCAGCCAGCCTGGCCATCTGTGAGA
  AGGCCAGTGGGTACCTGCGGGACAGCTTAGCCTCTACATCAACTGCCAGTTCCATTGACAAGGCCATGCA
  GCTGCTCCTGTGTGATCTACTTCTTGTGGCCCGCACCAGCCTATGGCGGCGCCAACAGTCAGCAGCTTCA
  GCCCAGGGAGCTCACGGTACCAGCAATGGACCCCAGGCCTCTGCTCTGGAGCTGCGTGGTTTCCAACATG
  ACCTGAGCAGCCTGAGGCGCTTGGCACAGAGCTTCCGGCCTGCTATGAGGAGGGTCTTCCTACATGAGGC
  CACAGCTCGGCTGATGGCAGGAGCAAGTCCTGCCCGGACACACCAGCTCCTGGACCGCAGTCTGCGGAGG
  CGGGCAGGTTCCAGTGGCAAAGGAGGCGCTGCAGCTGAGCTGGAGCCTCGACCCACATGGCGGGAGCACA
  CAGAGGCCTTGCTGTTGGCCTCCTGCTATCTGCCCCCTGCCTTCCTGTCGGCCCCCGGGCAGCGAGTGAG
  CATGCTGGCTGAGGCAGCGCGCACCGTGGAGAAGCTTGGCGATCACCGGCTCCTGCTTGACTGCCAGCAG
  ATGCTCCTGCGCCTGGGTGGCGGGACCACTGTCACTTCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217493 representing NM_001276707
  Red=Cloning site Green=Tags(s)
  
  MDELPFGEAALEQALAEVCEMDAALLTDIEDMLQLINNQDSDFPGLFDAPYAGGETGDTGPSSPGASSPE
  SFSSPASLGSSLEAFLGGPKVTPAPLSPPPSAPTAVKMYPSVPPFSPGPGIKEEPVPLTILQPPAPQPSP
  GTLLPPSFPPPPVQLSPAPVLGYSSLPSGFSGTLPGNTQQTPSSLPLGSTPGISPTPLHTQVQSSAAQQP
  PPASAAPRMSTVASQIQQVPVVLQPHFIKADSLLLTAVKTDTGATMKTAGINTLAPGTAVQAGPLQTLVS
  GGTILATVPLVVDTDKLPIHRLAAGGKALGSAQSRGEKRTAHNAIEKRYRSSINDKIVELKDLVVGTEAK
  LNKSAVLRKAIDYIRFLQHSNQKLKQENLTLRSAHKSKSLKDLVSACGSGGGTDVSMEGMKPEVVETLTP
  PPSDAGSPSQSSPLSLGSRGSSSGGSDSEPDSPAFEDNQVKAQRLPSHSRGMLDRSRLALCVLVFLCLTC
  NPLASLFGWGILTPSDASGVHRSSGRSMLEAESRDGSNWTQWLLPPLVWLANGLLVLACLALLFVYGEPV
  TRPHSGPAVHFWRHRKQADLDLARGDFAQAAQQLWLALQALGRPLPTSNLDLACSLLWNLVRHLLQRLWV
  GRWLAGQAGGLQRDYRLRKDARASARDAAVVYHKLHQLHAMGKYTGGHLVASNLALSALNLAECAGDAIS
  MATLAEIYVAAALRVKTSLPRALHFLTRFFLSSARQACLAQSGAVPLAMQWLCHPVGHRFFVDGDWAVHG
  APQESLYSVAGNPVDPLAQVTRLFCEHLLERALNCIAQPSPGAADGDREFSDALGYLQLLNSCSDAVGAP
  ACSFSVSSSMATTTGTDPVAKWWASLTAVVIHWLRRDEEAAERLYPLVEHIPQVLQETERPLPRAALYSF
  KAARALLDHRKVESSPASLAICEKASGYLRDSLASTSTASSIDKAMQLLLCDLLLVARTSLWRRQQSAAS
  AQGAHGTSNGPQASALELRGFQHDLSSLRRLAQSFRPAMRRVFLHEATARLMAGASPARTHQLLDRSLRR
  RAGSSGKGGAAAELEPRPTWREHTEALLLASCYLPPAFLSAPGQRVSMLAEAARTVEKLGDHRLLLDCQQ
  MLLRLGGGTTVTSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276707
• ORF Size: 3402 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001276707.1, NP_001263636.1
• RefSeq Size: 4377
• RefSeq ORF: 3405
• Locus ID: 78968
• MW: 120.5 kDa
• Gene Summary: transcription factor; binds to the sterol regulatory element 1; regulates the transcription of genes important for sterol biosynthesis [RGD, Feb 2006]