Plekha5 (NM_139340) Rat Tagged ORF Clone

CAT#: RR217575

Plekha5 (myc-DDK-tagged) - Rat pleckstrin homology domain containing, family A member 5 (Plekha5)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plekha5
• Synonyms: Pepp2; TRS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217575 representing NM_139340
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGACCTGAACCTGGAGTGGATTTGTTCGCTGCCGCGCTCCTGGACGTACGGGATCACTAGGG
  GCGGCCGCGTCTTCTTCATCAACGAGGAGGCCAAGAGCACCACCTGGCTGCACCCGGTCACCGGCGAGGC
  CGTGGTCACCGGGCACCGGCGGCAGAGCACAGATTTGCCCACTGGCTGGGAAGAAGCATATACATTTGAA
  GGCGCAAGATACTATATAAACCACAATGAAAGGAAAGTAACCTGCAAGCATCCAGTCACAGGACAGCCAT
  CCCAGGACAACTGTATCTTTGTCGTGAATGACCAGACTGTTGCAACCATGACATCTGAAGAAAAGAAGGA
  GCGACCCATAAGTATGATAAATGAAGCTTCCAATTACAACATGGCTTCAGACTATACTGTGCATCCTATG
  AGCCCCGTGGGCAGGACATCACGGGCCTCCAAAAAGGTTCATAATTTTGGGAAAAGGTCCAATTCAATTA
  AAAGGAACCCTAATGCCCCTGTGGTCAGGCGCGGCTGGCTGTATAAGCAGGACAGCACCGGCATGAAGCT
  GTGGAAGAAGCGCTGGTTTGTGCTTTCTGACCTTTGCCTTTTTTACTACAGAGATGAGAAGGAGGAGGGT
  ATTTTGGGAAGCATCCTGCTGCCCAGTTTTCAGATAGCCATGCTTACTGCCGAGGACCACATCAATCGCA
  AATATGCTTTTAAGGCAGCCCACCCAAACATGCGGACCTATTATTTCTGCACTGATACAGGCAAGGAAAT
  GGAGCTATGGATGAAGGCCATGTTAGATGCTGCTCTGGTGCAGACAGAACCTGTGAAAAGAATTACCTTT
  AATTTCCGAGTGGACAAGATTACAACAGAAAGTGCATCAACTAAAGAAACCAATAACATTCCCAACCACA
  GAGTCCTCATTAGACCTGAGGTCCAAAACAATCAGAAAAATAAGGAAATAAGCAAAATTGAAGAAAAAAG
  GGCCTTAGAAGCTGAAAGATACGGGTTTCAGAAGGATGGGCAAGATAGACCATTAACAAAAATTAACAGT
  GTAAAGTTGAACTCGCTGCCATCAGAATATGAAAGCGGGCCAGCCTGCCCACCTCAGAATATACACTACA
  GACCCATCAATGTGAATAATTCTGACAGCAAAGCAGTGAATGTCAGCTTGGCAGATGTCCGGGGTGGGAG
  TCACCCAAATACAGGGCCCCTAGCCACAGAAGCTGATCGAGTCATTCAGAGAACGAATTCAATGCAGCAG
  CTGGAGCAGTGGATTAAAGTTCAGAAAGGACGGGGTCTTGAAGAGGAGCCCAGAGGAGTAATTTCTTATC
  AAACATTGCCAAGAAATATGCCAAGCCACCGAGCCCAGATTCTGGCCCGCTACCCTGAAGGTTACCGAAC
  ACTCCCAAGAAACAGCAAGACCAGACCAGAGAGTATCTGCAGTGTAACGCCTTCTGGTCATGAGAAGACA
  GGGCCTGGGGCAGAGGAGAAGCGGCGGTCCATGAGGGATGACACCATGTGGCAGCTGTATGAGTGGCAGC
  AGCGCCAGTTTTACCACAAGCAGAGCACCCTCCCTCGGCACAGCTGCCTGAGCAGCCCTAAAGCCATGGG
  GAAGGTTTCTGACCAGACAATGCACTCCATCCCCACGTCACCGTCCCATGTGCCAGCTGCTGCTTACCAG
  GGCTTCTCCCCTCAGCGGACATACAGGTCAGAAGTGTCCTCGCCAATCCAGAGAGGAGATGTGACAATAG
  ACCGCAGGCACAGGCCTCATCACCCTAAGCATGTCTATGTACCTGACAGAAGGTCAATGCCGGCTGGCTT
  AGCCTTACAGGCTGTTAGTCCTCAGAGCCTCCACGGCAGGACGCCAGAGGAGCTTACGCTGCTGCTGATA
  AAGCTGAGACGGCAGCAAGCAGAGCTGAGTAGTATCCGGGAGCACACCTTAGCACAGCTCATGCAGCTGA
  AACTCGAGGCCCACAGCCCGAAGAATGAAATTCTTTCACATCATCTTCAAAGGAACACCATATATTTGGA
  TCATCAGATGAAAGAAAATGAACCTATTATCACCATGGTTCACACCATGATTGAGAACTCGGCGCTAAGA
  CCACAACTGTACCAACAATTCTTAAGACAGAAGAACAAGATAAGTCTCTATTGTCTCTCACAAGATGAGT
  GTAGAGGCACATTATACAAATACAGACCTGAGGAAGTAGATATCGATGCCAAATTAAGCCGATTGTGTGA
  ACAGGATAAGGTGGTCCGTGCACTGGAGGAGAAGCTGCAGCAGCTGCACAAGGAGAAATACACGCTTGAG
  CAAGCCTTGCTGTCAGCCAGCCAAGAGATAGAGATGAATGCAGATAACCCAGCCGCCATTCAGACGGTGG
  TGCTGCAGAGGGACGACCTGCAGAACGGGCTGCTGAGCACGTGTCGGGAACTCTCCCGAGCCACCGCTGA
  GCTGGAACGAGCCTGGAGAGAATATGACAAGTTAGAATATGATGTGACTGTTACCAGGAACCAGATGCAA
  GATCAGCTTGATCGCCTTGGAGAAGTGCAGAGTGAGTCGGCAGGAATTCAACGGGCACAGATTCAAAAAG
  AACTTTGGAGAATTCAAGATGTCATGGAGGGGTTGAGCAAACACAAACAGCAAAGAGGCTCTTCAGAAAC
  AGGTCTGGCAGGACCAAAGCCTTTCCCAGCAGTTAAGTACAAAAGCGAGGAAGAGGAAGTAGTCCCACCT
  CGGCCTCCACTTCCTCGGTCTTATGACTTTACAGAGCAGCCTCCCATAATCCCCCCTCTGCCCAGTGATA
  GCAGCTCCTTGCTCTGTTATAGCAGGGGCCCCGTGCATCTGCCTGAAGAAAAGAAGAGTCACCAAGTTCA
  AGGATATCCAAGAAATGGATCTCACTGTGGTCCAGATTATAGACTCTACAAGAGCGAGCCAGAGTTGACC
  ACAGTGGCAGAAGTTGATGAATCTAATGGAGAAGAAAAATCAGAGCCAGCTTCTGAGGCAGAAGCTCCGG
  TTGTTAGAGGTTCCCACTTTCCAGTTGGAGTTCCTCTGAGAACGAAGTCACCTACACCTGAGTCCTCAAC
  GATAGCTTCCTATGTGACCTTGAGGAAGACGAAGAAGATGGTGGAGCTAAGAACGGAAAGACCGAGAAGT
  GCCGTGGAACAGCTCTGTCTGGCGGAAAGTACTCGGCCTCGGATGACTGTGGAAGAGCAGCTGGAGAGAA
  TCAGGAGGCACCAGCAGGCCTGCCTGAGGGAGAAGAAGAAGGGGCTGAACGTCCTCAGTGCATCCGACCC
  ATCTCCCTCACAAAGTCCTTCAAGCGTGAGGGACAGTCCTTCCAGAGTTCCTCAGACTGTAAGGAGGGAT
  GATAACACTAAGGAGCTAGACGCCGTCCACAGAGAAAATGATGGAAAGCCAGACTATGACACTTCAGCAG
  CAGAAACTGCTCACCCAGAAGAAGCTGAACCCCAAAGTGCAGATGTCGGCCGAAAGCTAAAGAGAACTGA
  AAGTATTTTTTATGAAATGCTTTACACGCCTGAACCTTTAAATGGACTGACTTCTGAGGAAGTGATGGCT
  AAGGAGAGGCAGGAAGAGCAGGTGCCTGAAGGTGCCGTGTGCAGCCTTCAGGAAGAGGCTGCCATGGCAA
  ACCATCAGGCAGAAGGCCCCCCTGAGGAAGCAGAGAGTCTTCATGAAGAGGAGGAAGCTCTGGCTTCCTG
  TGAGCCAGCTCCTGAGACTCCTAGGGAGAATCAAACCACAGTGAAAAGTCTGTCTCCGTCTCCTGACTCC
  TCCACGGCAGCAGACCCGCCCACTCCGCCCCAGCTCACAGAAGGATCACATTTCATGTGTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217575 representing NM_139340
  Red=Cloning site Green=Tags(s)
  
  MAADLNLEWICSLPRSWTYGITRGGRVFFINEEAKSTTWLHPVTGEAVVTGHRRQSTDLPTGWEEAYTFE
  GARYYINHNERKVTCKHPVTGQPSQDNCIFVVNDQTVATMTSEEKKERPISMINEASNYNMASDYTVHPM
  SPVGRTSRASKKVHNFGKRSNSIKRNPNAPVVRRGWLYKQDSTGMKLWKKRWFVLSDLCLFYYRDEKEEG
  ILGSILLPSFQIAMLTAEDHINRKYAFKAAHPNMRTYYFCTDTGKEMELWMKAMLDAALVQTEPVKRITF
  NFRVDKITTESASTKETNNIPNHRVLIRPEVQNNQKNKEISKIEEKRALEAERYGFQKDGQDRPLTKINS
  VKLNSLPSEYESGPACPPQNIHYRPINVNNSDSKAVNVSLADVRGGSHPNTGPLATEADRVIQRTNSMQQ
  LEQWIKVQKGRGLEEEPRGVISYQTLPRNMPSHRAQILARYPEGYRTLPRNSKTRPESICSVTPSGHEKT
  GPGAEEKRRSMRDDTMWQLYEWQQRQFYHKQSTLPRHSCLSSPKAMGKVSDQTMHSIPTSPSHVPAAAYQ
  GFSPQRTYRSEVSSPIQRGDVTIDRRHRPHHPKHVYVPDRRSMPAGLALQAVSPQSLHGRTPEELTLLLI
  KLRRQQAELSSIREHTLAQLMQLKLEAHSPKNEILSHHLQRNTIYLDHQMKENEPIITMVHTMIENSALR
  PQLYQQFLRQKNKISLYCLSQDECRGTLYKYRPEEVDIDAKLSRLCEQDKVVRALEEKLQQLHKEKYTLE
  QALLSASQEIEMNADNPAAIQTVVLQRDDLQNGLLSTCRELSRATAELERAWREYDKLEYDVTVTRNQMQ
  DQLDRLGEVQSESAGIQRAQIQKELWRIQDVMEGLSKHKQQRGSSETGLAGPKPFPAVKYKSEEEEVVPP
  RPPLPRSYDFTEQPPIIPPLPSDSSSLLCYSRGPVHLPEEKKSHQVQGYPRNGSHCGPDYRLYKSEPELT
  TVAEVDESNGEEKSEPASEAEAPVVRGSHFPVGVPLRTKSPTPESSTIASYVTLRKTKKMVELRTERPRS
  AVEQLCLAESTRPRMTVEEQLERIRRHQQACLREKKKGLNVLSASDPSPSQSPSSVRDSPSRVPQTVRRD
  DNTKELDAVHRENDGKPDYDTSAAETAHPEEAEPQSADVGRKLKRTESIFYEMLYTPEPLNGLTSEEVMA
  KERQEEQVPEGAVCSLQEEAAMANHQAEGPPEEAESLHEEEEALASCEPAPETPRENQTTVKSLSPSPDS
  STAADPPTPPQLTEGSHFMCV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139340
• ORF Size: 3843 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_139340.2, NM_139340.1, NP_647556.1
• RefSeq Size: 4790
• RefSeq ORF: 3846
• Locus ID: 246237
• MW: 145.6 kDa
• Gene Summary: member of homeobox PEPP subfamily, preferentially expressed in reproductive tissues; human homolog specifically expressed in testis [RGD, Feb 2006]