Top2b (NM_001100858) Rat Tagged ORF Clone

CAT#: RR217667

Top2b (myc-DDK-tagged) - Rat topoisomerase (DNA) II beta (Top2b)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Top2b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217667 representing NM_001100858
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGTCCAGCCTCGCGGGCGTTGACGGGGCGCTGACCTGGGTGAACAATGCTGCAAAAAAGGAAG
  AGTTGGAGACAGCGAACAAAAATGATTCTTCCAAGAAGTTGTCTGTTGAGAGAGTGTACCAGAAGAAGAC
  CCAACTGGAGCATATTCTCCTTCGTCCTGATACATACATTGGATCAGTGGAACCACTGACACAGTTAATG
  TGGGTGTATGACGAAGATGTAGGCATGAACTGCAGAGAGGTTACCTTTGTTCCGGGCTTATACAAGATCT
  TTGATGAAATTTTGGTCAATGCTGCTGACAACAAGCAGAGGGATAAGAACATGACCTGTATTAAGGTTTC
  TATTGATCCTGAATCTAATATTATAAGCATTTGGAATAATGGAAAAGGCATTCCAGTAGTAGAACACAAA
  GTAGAGAAAGTTTATGTTCCTGCTTTAATTTTTGGACAGCTTTTAACATCGAGTAATTACGATGATGATG
  AGAAAAAAGTTACAGGAGGCCGTAATGGTTATGGTGCAAAACTTTGTAATATTTTTAGTACAAAGTTTAC
  AGTAGAAACGGCTTGCAAAGAATACAAACATAGTTTTAAGCAGACATGGATGAATAATATGATGAAGACT
  TCTGAAGCCAAAATCAAACATTTTGATGGGGAAGATTATACATGCATAACATTCCAGCCAGATCTAGCCA
  AGTTTAAGATGGAAAAACTTGACAAGGACATTGTGGCCCTCATGACCAGAAGGGCATACGATTTAGCTGG
  TTCCTGCAAAGGCGTCAAGGTCATGTTCAATGGCAAGAAGTTGCCTGTAAATGGATTTCGCAGCTATGTA
  GACCTTTATGTGAAAGACAAATTGGATGAAACTGGGGTCGCTCTCAAAGTTATTCATGAACTTGCAAATG
  AAAGATGGGATGTTTGTCTAACCTTGAGTGAAAAAGGTTTCCAGCAGATCAGCTTTGTGAATAGTATTGC
  AACTACAAAAGGTGGACGGCACGTGGATTACGTAGTAGATCAAGTTGTTGGTAAACTGATTGAAGTAGTT
  AAGAAAAAGAACAAAGCTGGTGTATCAGTGAAGCCATTTCAAGTTAAAAACCACATATGGGTTTTTATTA
  ATTGTCTTATTGAAAATCCAACCTTTGATTCTCAAACTAAGGAAAACATGACTCTGCAGCCCAAGAGCTT
  TGGGTCTAAATGCCAGCTGTCAGAAAAGTTCTTTAAAGCGGCCTCTAATTGTGGCATTGTAGAAAGCATA
  TTGAACTGGGTGAAATTTAAGGCTCAGACTCAGCTGAATAAGAAGTGCTCCTCAGTAAAGTACAGCAAAA
  TCAAAGGCATTCCCAAACTGGATGATGCCAATGATGCTGGTGGCAAACATTCCCTGGAGTGTACATTAAT
  ATTAACAGAGGGGGACTCTGCCAAATCTCTAGCTGTCTCTGGATTAGGTGTGATTGGGAGAGACAGATAT
  GGAGTCTTCCCTCTCAGGGGTAAAATTCTCAATGCACGAGAAGCGTCTCATAAACAGATTATGGAAAATG
  CAGAAATAAACAATATTATTAAAATAGTTGGTTTGCAATACAAGAAAAGTTATGATGATGCAGAATCTCT
  GAAAACTTTACGTTATGGGAAGATTATGATTATGACTGATCAGGATCAAGATGGTTCTCACATTAAAGGC
  CTGCTAATCAATTTTATTCATCACAATTGGCCATCACTTTTGAAGCATGGCTTTCTTGAAGAATTCATTA
  CTCCCATTGTAAAGGCGAGCAAAAATAAGCAGGAACTTTCCTTTTATAGTATTCCTGAATTTGATGAGTG
  GGAAAAACATATAGAAAACCAGAAAGCCTGGAAAATAAAGTACTACAAAGGATTGGGTACCAGTACAGCT
  AAGGAAGCCAAGGAATATTTTGCTGATATGGAAAGGCATCGCATTTTGTTTAGATATGCTGGCCCTGAGG
  ATGATGCTGCCATTACCTTGGCATTTAGTAAGAAGAAGATTGATGACAGAAAAGAATGGTTAACCAACTT
  TATGGAGGATCGGAGACAGCGGAGGTTACATGGCCTACCAGAGCAATTTTTATATGGTACAGCAACTAAG
  CATTTGACATACAATGATTTCATCAACAAGGAATTGATTCTCTTCTCAAACTCGGACAATGAGAGATCTA
  TACCCTCTCTTGTGGATGGCTTCAAGCCAGGTCAACGAAAAGTTTTGTTTACCTGTTTCAAGAGGAATGA
  TAAACGTGAAGTTAAAGTCGCACAGCTGGCTGGATCTGTTGCTGAAATGTCTGCTTATCATCATGGAGAA
  CAAGCGTTGATGATGACTATTGTGAATTTGGCTCAGAATTTTGTGGGCAGCAACAACATTAACTTGCTTC
  AGCCCATTGGTCAGTTTGGAACTCGGCTTCACGGTGGCAAAGATGCTGCAAGCCCCCGTTATATCTTCAC
  AATGTTAAGCTCTCTGGCAAGACTACTTTTTCCAGCTGTGGATGATAACTTGCTTAAGTTCCTTTATGAT
  GATAATCAGCGTGTAGAGCCGGAGTGGTATATCCCCATAATCCCCATGGTTTTGATTAATGGTGCTGAGG
  GTATTGGGACTGGATGGGCTTGTAAATTGCCCAACTATGATGCCAGAGAAATTGTGAACAATGTCAGGCG
  GATGCTAGAAGGTTTGGATCCTCACCCCATGCTTCCAAACTATAAGAACTTCAAAGGCACCATCCAGGAG
  CTTGGTCAGAACCAGTATGCTGTTAGTGGTGAGATATTTGTGGTGGACAGAAACACAGTAGAGATTACAG
  AACTTCCAGTTAGAACCTGGACACAGGTATACAAGGAGCAGGTCCTAGAACCTATGCTTAATGGAACAGA
  TAAAACACCAGCACTGATTTCTGATTACAAAGAATATCACACTGATACAACTGTGAAATTTGTGGTGAAG
  ATGACAGAAGAGAAGCTCGCCCAAGCGGAAGCTGCCGGCCTGCATAAAGTTTTTAAACTCCAAACCACTC
  TTACTTGCAATTCCATGGTACTCTTTGATCATATGGGATGTCTGAAGAAGTATGAAACTGTGCAAGACAT
  TTTGAAAGAATTCTTTGATTTACGGTTAAGTTATTATGGTTTACGGAAGGAGTGGCTTGTAGGAATGTTG
  GGAGCAGAATCCACAAAGCTTAACAATCAAGCCCGTTTCATTTTAGAGAAGATACAAGGGAAAATCACGA
  TAGAGAATAGGTCAAAGAAAGATTTGATTCAAATGTTAGTCCAGAGAGGTTATGAATCTGACCCAGTGAA
  AGCCTGGAAAGAAGCACAAGAAAAGGCTGCGGAAGAGGAAGATGCACAAAACCCACATGATGACAGTTCC
  TCTGACTCAGGAACTCCTTCAGGCCCTGATTTTAATTATATTTTAAATATGTCTCTATGGTCTCTTACTA
  AAGAAAAAGTTGAAGAACTCATTAAACAGAGAGATACAAAAGGACGAGAGGTTAATGATCTCAAAAGGAA
  ATCTCCCTCAGATCTTTGGAAAGAAGATTTAGCAGCATTTGTTGAAGAATTGGATAAAGTGGAAGCACAA
  GAACGAGAAGACATTCTGGCAGGGATGTCTGGGAAAGCAGTGAAAGGCAAAGTCGGCAAAGCTAAAGTAA
  AGAAGCTCCAGCTGGAGGAGACGATGCCCTCGCCTTACGGGAGAAGAATCGTTCCTGAGATTACTGCCAT
  GAAGGCAGACGCCAGCAGAAAACTGCTGAAGAAGAAGAAGGGTGATCCTGATACTACAGTTGTGAAAGTA
  GAATTTGATGAAGAATTTAGTGGAACACCAGCAGAAGGTACAGGAGAAGAGACATTGACACCATCTGCTC
  CTGTAAATAAAGGCCCCAAACCCAAAAGGGAGAAAAAGGAGCCTGGTACCAGGGTTAGAAAAACACCTGC
  ATCAACTGGCAAACCTAATACAAAGAAAGTGAAGAAGCGGAATCCTTGGTCAGATGATGAGTCCAAATCA
  GAGAGTGACTTGGAAGAAGCAGAGCCTGTGGTTATCCCAAGAGATTCTTTGCTTAGGAGAGCAGCTGCTG
  AAAGACCTAAGTACACTTTTGATTTCTCAGAAGAGGAGGAGGATGATGCTGATGATGATGACGACAATAA
  TGATTTAGAAGAATTGAAAGTTAAAGCATCTCCCATAACAAATGATGGGGAGGATGAATTTGTTCCTTCA
  GATGGCATAGATAAAGATGAGTATGCCTTTTCTCCAGGCAAATCCAAAGCTACACCAGAAAAATCTTCAC
  ATGACAAAAAAAGTCAGGATTTTGGAAATCTTTTTTCATTTCCTTCATATTCTCAGAAGTCAGAAGATGA
  TTCAGCTAAATTTGACAGTAATGAAGAAGACACCACATCTGTTTTTGCACCATCATTTGGTCTGAAGCAA
  ACAGATAAAGTTCCAAGTCAAACAGTCGCTGCTAAAAAGGGTAAAGCACCTTCAGATGCAGCAGCCCCTA
  AGGCCAAGAGAGCCCCTAGACAGAGGAAGGTAGTAGAGCCTGCAAACTCCGACTCGGATTCTGAACTCGG
  CAACATTCCAAAGAAGACGGCCGCACCGAAAGGTAAAGGCCGAGGGGCAAAGAAAAGGAAAGCATCAGGC
  TCAGAAAATGAAGGTGATTATAACCCTGGCAGGAAACCATCTAAAACAGCCAGCAAGAAACCGAAGAAAA
  CGTCTTTTGATCAGGATTCAGATGTAGACATCTTCCCTTCAGACTTCACTTCTGAACCACCTGCTCTCCC
  ACGGACTGGTCGGGCTAGGAAAGAAGTAAAATATTTTGCAGAGTCTGATGAAGAAGAAGACGTTGATTTT
  GCAATGTTTAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217667 representing NM_001100858
  Red=Cloning site Green=Tags(s)
  
  MAKSSLAGVDGALTWVNNAAKKEELETANKNDSSKKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLM
  WVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHK
  VEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKT
  SEAKIKHFDGEDYTCITFQPDLAKFKMEKLDKDIVALMTRRAYDLAGSCKGVKVMFNGKKLPVNGFRSYV
  DLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVV
  KKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESI
  LNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRY
  GVFPLRGKILNAREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKG
  LLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWEKHIENQKAWKIKYYKGLGTSTA
  KEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATK
  HLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGE
  QALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSSLARLLFPAVDDNLLKFLYD
  DNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQE
  LGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVK
  MTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGML
  GAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDAQNPHDDSS
  SDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELDKVEAQ
  EREDILAGMSGKAVKGKVGKAKVKKLQLEETMPSPYGRRIVPEITAMKADASRKLLKKKKGDPDTTVVKV
  EFDEEFSGTPAEGTGEETLTPSAPVNKGPKPKREKKEPGTRVRKTPASTGKPNTKKVKKRNPWSDDESKS
  ESDLEEAEPVVIPRDSLLRRAAAERPKYTFDFSEEEEDDADDDDDNNDLEELKVKASPITNDGEDEFVPS
  DGIDKDEYAFSPGKSKATPEKSSHDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDTTSVFAPSFGLKQ
  TDKVPSQTVAAKKGKAPSDAAAPKAKRAPRQRKVVEPANSDSDSELGNIPKKTAAPKGKGRGAKKRKASG
  SENEGDYNPGRKPSKTASKKPKKTSFDQDSDVDIFPSDFTSEPPALPRTGRARKEVKYFAESDEEEDVDF
  AMFN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100858
• ORF Size: 4842 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001100858.1, NP_001094328.1
• RefSeq Size: 4845
• RefSeq ORF: 4845
• Locus ID: 361100
• MW: 182 kDa
• Gene Summary: a nuclear enzyme that catalyzes the breaking and rejoining of two strands of duplex DNA [RGD, Feb 2006]