Ralgapa1 (NM_020083) Rat Tagged ORF Clone

CAT#: RR217729

Ralgapa1 (myc-DDK-tagged) - Rat Ral GTPase activating protein, alpha subunit 1 (catalytic) (Ralgapa1)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ralgapa1
• Synonyms: Garnl1; GRIPE; p240; Tulip1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217729 representing NM_020083
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTCTAAGAAGCCGCACGGGGACGTGAAGAAGTCCACCCAGAAGGTGCTGGACACCAAGAAGGACG
  CGCTGACCCGCCTCAAGCACCTGCGCATCGTCATCGAGAATGCAGACTCTATTGACCTTAAACAGTTCTT
  TGACCAGCATTTTTCACATATATACTATGTGTTTTTTGAAAATTTTGTGACTATTGAAGCCAGTCTTAAA
  CAGAAAGGTCACAAGTCTCAAAGAGAGGAATTAGATGCTATACTTTTTATTTTTGAGAAAATTTTACAAC
  TTCTTCCAGAAAGAATTCATCAGCGATGGCAATTCCATAGTATTGGGTTGATTTTAAAGAAACTACTGCA
  CACAGGAAACTCCTTAAAGATCAGACGGGAAGGTGTTCGTCTCTTCTTGTTATGGTTGCAAGCTCTTCAG
  GATAACTGTAGCAAAGAGCAGCTCTGGATGTTTTCATGCTTGATCCCTGGATTTTCAGCACCACAATCTG
  AATATGGACCTCGAACTTTAGATAATCTTATTAATCCTCCACTCAACCTTCAAGAAACTCAAGTAACTAT
  AGAGGAAGTCACCCCTCTTGTTCCCCCGCAGTCAGGAGACAAAGGACAAGAAGACCTCACAAGCTATTTT
  CTTGAAGCACTTTTAAAATACATAGTAATTCAGGTAAAAAGTTTAGAATGGAAGAACAAAGAAAACCAAG
  AAAGGGGATTTTCATTTTTGTTTTCACATTTTAAGAAGTTTTACTTGCCTTATATTTTCCCAAACCTCTG
  CAAGGAGAATAGTTTGTATCATCCTGTACTTGATATCCCACAGGTCAGACCAAAGCCTCATTATGTGATG
  GTAAAGAAGGATGCTGAAACCAATGAAACGATATACTGTACAAAAGAGCCTTTCATCCAGGCTCGGGTCA
  TTGTCATTCGTTGGCTGGTTTCTTTCTGGCTGGAGCCAAAACCGCATTCAGGACCTAATATTCCTGGGAT
  GGAGGGTGAAGTCTTGCCAAAGAATATTCAGAGAGCAGCTGCTAGTTTGGTCTCCAGAGAAGAAAGCAAA
  AATGATACTGTTGATAAAGCAGACAAAACCGCAGAGCCCGAGCAGTCGCATTCCAATACAAGCACTCTCA
  CAGAGCGAGAGCCCAGCTCTTCCAGTCTCTGTAGCATCGATGAAGAACACCTCACGGACATTGAAATCGT
  TCGCAGGGTATTTTCATCTAAGAGAAGCAATGTAAACTTTGTCACAGAGATATTTCGTCAGGCATTTTTA
  TTACCAATTTGTGAAGCAGCAGCTATGAGAAAAGTGGTAAAAGTGTATCAAGAGTGGATTCAACAGGAGG
  GAAAACCTTTGTTTATGCAAGAGCCTGAAGAAACTGTGATCACTTCTTCAGATATACCTTGCAGTGAAAA
  TGTCACAGACCATGACATTTCAATAGAAGACGGAGAGAAGAGAGAAGAGGAAAATGGGACCAATATTTCT
  GAACATGTTCGAAATTCTACCTGGACAAAGAATGGTTCCTACCAGGAAGCTTTTCATGTCTCTGAAGAAG
  CCACAGAACAAAATATTCAAGCTGGTACTCAGGCTGTTTTACAGGTGTTCATTATAAACTCATCAAACAT
  ATTTCTTCTTGAACCTGCAAATGAAATAAAAAATCTTCTGGATGAGCACACAGATATGTGTAAACGTATT
  CTTAACATATATCGATACATGGTTGTGCAAGTATCAATGGACAAAAAGACCTGGGAACAGATGCTACTCG
  TGTTGCTCAGAGTCACGGAGTCTGTACTGAAGATGTCGTCACAAGCTTTTCTGCAGTTCCAGGGGAAAAA
  AAACATGACCCTGGCAGGTCGACTTGCAGGACCACTTTTCCAGACTCTCATTGTTGCCTGGATCAAAGCA
  AATCTAAATGTATACATTTCTCGAGAACTTTGGGATGACTTACTTTCAGTATTGTCATCGTTGACCTATT
  GGGAAGAATTGGCCACTGAGTGGTCGCTGACCATGGAGACACTAACTAAAGTTTTAGCTAGGAATTTATA
  TAGTTTGGATCTCAGTGATTTGCCATTGGATAAGCTCAGTGAACAGAAACAAAAAAAGCACAAAGGGAAA
  GGAGTTGGACATGAATTTCAGAAAGTTTCAGTTGACAAGTCATTTTCTAGAGGATGGAGTCGCGATCAGC
  CTGGCCAGGCACCCATGAGACAGAGAAGTGCAACGACTACTGGCTCCCCGGGAACTGAGAAGGCCAGGAG
  TATAGTACGGCAAAAAACTGTTGATATTGATGATTCTCAAATCCTTCCCCGCTCAACCAGAGTCAGACAT
  TTTTCACAGAGTGAAGACACTGGTAATGAAGTTTTTGGTGCTTTGCATGAGGAACAGCCACTGCCTCGAA
  GTAGCAGCACTTCTGACATCTTGGAACCATTCACTGTTGAGCGAGCCAAAGTAAATAAAGAGGACACGAG
  CCCCAAACTGCCTCCTCTTAATAGTGAGACTGGCGGCAGCAGCGCTAATGTTCCTGACCTGATGGATGAG
  TTTATCGCAGAGCGACTTCGAAGTGGCAATGCCTCGACTATGACAAGAAGGGGAAGTAGTCCAGGCAGCC
  TTGAAATTCCGAAAGACCTTCCTGATATTCTAAACAAGCAGAATCAGATGCGCCCTGTTGATGACCCTGG
  TGTGCCCTCAGAATGGACGTCTCCTGCCAGTGCAGGGAGCAGTGATCTCATGAGCTCAGATAGTCACTCG
  GATTCCTTCAGTGCCTTCCAGTGTGAGGGCCGAAAATTTGACAACTTTGGCTTCGGGACTGACATTGGGA
  TCCCATCTTCTGCTGATGTGGATTCAGGCTCTGGCCATCATCAAAGCACTGAGGAGCAGGAAGTGGCTAG
  CCTAACGACGCTTCATTTAGACTCAGAGACAAGCAGCCTTAATCAACAAGCATTCTCTGCTGAAGTTGCA
  ACTGTCACTGGTTCAGAAAGTGCTTCCCCTGTTCATTCAGCTCTGGGGTCCAGGTCACAGACACCTTCCC
  CTTCTACGCTGAATATAGATCACATGGAACAGAAGGATCTACAGCTTGATGAGAAGCTCCACCACTCTGT
  TCTTCAGACGCCAGATGACCTAGAAATCAGTGAATTTCCTTCTGAATGTTGTAGTGTGATGGCAGGAGGT
  ACGCTCACTGGGTGGCATGCTGATGTTGCTACTGTTATGTGGCGAAGAATGCTTGGCATTTTGGGAGATG
  TCAATGCTATTATGGATCCTGAAATACATGCTCAAGTTTTTGATTACCTCTGTGAACTTTGGCAGAATCT
  AGCCAAGATCAGAGATAATCTTGGCATTTCAGCCGATAACCTGACTTCACCTTCTCCACCTGTTTTGATT
  CCTCCACTAAGAATTCTTACACCTTGGCTTTTTAAGGCAACCATGTTGACTGATAAGTATAAACAAGGTA
  AATTACATGCATATAAACTTATTTGTAATACAATGAAGAGAAGACAAGATGTATCCCCAAATAGAGATTT
  TTTAACGCATTTCTACAATATAATGCATTGTGGATTGCTTCATATTGACCAGGACATCGTCAATACGATC
  ATCAAGCACTGCTCACCTCAGTTTTTTTCACTTGGTTTGCCTGGGGCCACAATGCTTATTATGGATTTTA
  TTATAGCGGCTGGGAGAGTGGCTTCTTCAGCTTTTCTTAATGCACCAAGAGTGGAAGCACAGGTTCTTCT
  GGGATCTTTGGTTTGCTTCCCCAACTTATACTGTGAGCTGCCTGCTCTGCATCCAAGCACGCCTGACATC
  GCTGTGTCTCAGTTCACAGACGTCAAGGAACTTATAATTAAAACTGTCTTAAGCTCGGCAAGAGACGAAC
  CCTCTGGCCCTGCACGATGTGTAGCACTTTGCAGTTTAGGTATATGGATTTGTGAAGAATTAGTCCACGA
  ATCTCATCATCCTCAAATTAAAGAAGCACTGAATGTAATTTGTGTTTCATTAAAGTTTACTAATAAAACA
  GTTGCCCATGTAGCTTGTAACATGCTTCACATGCTGGTTCATTATGCACCTAGACTTCAGACTTACCAGC
  CTGACTCTCCCTTGAAAATTATTCAGATCCTAATAGCAACTATCACACACCTTTTGCCAAGTACAGAAGC
  TTCATCATATGAAATGGACAAGAGGTTGGTTGTGTCATTACTTCTTTGCCTTCTGGACTGGATCATGGCC
  TTACCTTTAAAGACAATACTCCAATCAGTCCATTCTACAGGAGCAGAAAATGAGAAGACAGAAAAATCTG
  TCCTCAACTGTATTTATAAGGTTTTACATGGGTGTGTATATGGAGCACAAAGTTTTAGCCATCCAAAGTA
  CTTTCCAATAAGCCTTTCTGATTTGGCATCTGTAGATTATGACCCTTTTATGCATTTGGAAAGTCTGAGA
  GAGCCTGAACCTCTACACTCTCCTGATTCAGAACGGTCTTCTAAACTGCAGCCGGTGACAGAAGTGAAAA
  CTCAGATGCAACAAGGATTAATTTCTATAGCAGCCCGTACTGTTATTACCCATCTGGTGAATCACCTGGG
  CCATTATCCAATGAGTGGTGGTCCTGCTATGCTAACGAGTCAGGTGTGTGAGAATCATGACAATCATTAT
  AGCGAAAGTACTGAACTTTCTCCTGAGCTCTTTGACAGTCCAAATATTCAGTTCTTTGTGCTGAATAATA
  CAACCTTAGTGTCTTGTATCCAGATAAGATCAGAAGAGAGTGTGCCTGGAGGAGGTTTAGCTGCTGGCCT
  TGTGTCAGCCAACTCAAATGTTAGAATCATAGTACGTGATCTCTCTGGAAAGTACTCATGGGATTCTGCC
  ATACTGTATGGGCCACCCATTGTAAGTGGTTTGCCTGAACCTACATCTTTCATACTTTCAATGTCTTACC
  AAGAGAAACCAGAAGAGCCTCCTACATCTAATGAATGCTTAGAAGACATAACAGTAAAAGATGGACTTTC
  TCTCCAGCTTAGAAGATTTAGAGAAACTGTTCCAACTTGGTCTACAATAAGAGAGGAGGAAGATGTTCTT
  GATGAGCTCCTGCAGTATTTAGGCACTACAAGTCCTGAATGTTTACAGAGAACTGGAATCTCCCTCAATG
  TTCCTGCTCCACAACCTGTGTGCATTTCTGAAAAACAGGAGAATGATGTCATTAATGCTATCCTTAAGCA
  ATACACAGAAGAAAAAGAGTTTGTAGAAAAGCACTTTAATGACTTAAACATGAAAGCCTCAGAACAAGAT
  GAACCAACACCTCAGAAACCTCAGTCAGCATTTTATTACTGCAGATTGCTTCTTAGTATATTGGGAATGA
  ATTCCTGGGACAAACGGAGGAGCTTTCATCTCCTGAAGAAAAATGAAAAGCTGCTCAGAGAACTGAGGAA
  CTTGGATTCAAGGCAGTGCCGAGAAACACACAAGATTGCAGTATTTTACGTTGCTGAAGGACAAGAAGAT
  AAATACTCCATTCTCACCAATATAGGAGGAAGCCAAGCATATGAAGATTTTGTTGCTGGCCTTGGTTGGG
  AGGTAAACCTTACAAATCACTGTGGCTTCATGGGAGGACTGCAGAAGAACAAAAGCACTGGACTGACCAC
  TCCATATTTTGCTACCTCTACAGTAGAAGTAATATTTCATGTATCTACAAGAATGCCTTCTGAATCTGAT
  GACTCTCTGACCAAAAAATTGAGACATCTAGGAAATGATGAAGTACACATTGTTTGGTCTGAACATACTA
  GAGACTACAGGAGAGGAATTATTCCTACAGAGTTTGGTGATGTCCTCATTGTAATATATCCAATGAAAAA
  CCACATGTTCAGTATCCAGATCATGAAAAAGCCAGAGGTTCCCTTCTTTGGTCCCCTTTTTGATGGTGCT
  ATTGTGAATGGAAAAGTTCTGCCCATTATGGTTCGATCCACAGCAATAAATGCTAGCCGTGCTCTGAAAT
  CACTGATTCCATTGTATCAAAACTTCTACGAGGAGAGAGCACGGTACCTGCAGACAATTGTCCAGCACCA
  CTTAGAGCCAACGACATTTGAAGACTTTGCAGCCCAGGTTTTCTCTCCAGCTCCCTACCATCATTTCCCT
  GCTGATGCAGATCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217729 representing NM_020083
  Red=Cloning site Green=Tags(s)
  
  MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENADSIDLKQFFDQHFSHIYYVFFENFVTIEASLK
  QKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQ
  DNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEVTPLVPPQSGDKGQEDLTSYF
  LEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKFYLPYIFPNLCKENSLYHPVLDIPQVRPKPHYVM
  VKKDAETNETIYCTKEPFIQARVIVIRWLVSFWLEPKPHSGPNIPGMEGEVLPKNIQRAAASLVSREESK
  NDTVDKADKTAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFL
  LPICEAAAMRKVVKVYQEWIQQEGKPLFMQEPEETVITSSDIPCSENVTDHDISIEDGEKREEENGTNIS
  EHVRNSTWTKNGSYQEAFHVSEEATEQNIQAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKRI
  LNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMSSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKA
  NLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGK
  GVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDSQILPRSTRVRH
  FSQSEDTGNEVFGALHEEQPLPRSSSTSDILEPFTVERAKVNKEDTSPKLPPLNSETGGSSANVPDLMDE
  FIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPVDDPGVPSEWTSPASAGSSDLMSSDSHS
  DSFSAFQCEGRKFDNFGFGTDIGIPSSADVDSGSGHHQSTEEQEVASLTTLHLDSETSSLNQQAFSAEVA
  TVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGG
  TLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISADNLTSPSPPVLI
  PPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTI
  IKHCSPQFFSLGLPGATMLIMDFIIAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPSTPDI
  AVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKT
  VAHVACNMLHMLVHYAPRLQTYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMA
  LPLKTILQSVHSTGAENEKTEKSVLNCIYKVLHGCVYGAQSFSHPKYFPISLSDLASVDYDPFMHLESLR
  EPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHY
  SESTELSPELFDSPNIQFFVLNNTTLVSCIQIRSEESVPGGGLAAGLVSANSNVRIIVRDLSGKYSWDSA
  ILYGPPIVSGLPEPTSFILSMSYQEKPEEPPTSNECLEDITVKDGLSLQLRRFRETVPTWSTIREEEDVL
  DELLQYLGTTSPECLQRTGISLNVPAPQPVCISEKQENDVINAILKQYTEEKEFVEKHFNDLNMKASEQD
  EPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQED
  KYSILTNIGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSESD
  DSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGA
  IVNGKVLPIMVRSTAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHFP
  ADADH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020083
• ORF Size: 6105 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020083.3, NM_020083.2, NP_064468.3
• RefSeq Size: 7476
• RefSeq ORF: 6108
• Locus ID: 56785
• MW: 229.5 kDa
• Gene Summary: mouse homolog interacts with the HLH region of E12 and may be involved in neuronal differentiation [RGD, Feb 2006]