Espl1 (NM_001170602) Rat Tagged ORF Clone

CAT#: RR217737

Espl1 (myc-DDK-tagged) - Rat extra spindle pole bodies homolog 1 (S. cerevisiae) (Espl1)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Espl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217737 representing NM_001170602
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAACTTCAAAGGAGTCAAGTTCGCCACTCTACTTTGCTGCAAGAGAGAGACCCAGGAGTTGCTGC
  CTGACTTGAAGGAGTTCCTGTCTAGATCTCGAACTGATTCAGTCAGCAGCAGAACCGATGCTGAGAGAAG
  ACAGGTCTGTGACACCATCCTGAGGGCTTGCACCCAGCAGCTGACTGCCAAGCTTGACGGCCCTGGTCAC
  CTGAGAAGTATCCTGGACCTGGCAGAGCTGGCCTGTGACGGCTACTTGTTGTCTACCCCACAGCGCCCTC
  CCCTCTATCTGGAACGCATTCTTTTTATCTTACTTCGGAATGGTTTGACTCAGGGAAACCCAGACGCTGT
  GCTCCGCCTTGCCCAGCCTCTCCATGCCTGCTTGGTACAGAACTCTCGAGAGGCTGCCCCTCAGGACTAT
  GAGGCTGTGACCCGGGGTACTTTTTCTCTGCTTTGGAAGGGAGCAGAAGCCCTGTCAGAGCGGCGAGCTG
  CATTCTCAGCTAGGCTGAAGGCCTTGAGTTTCTTAGTGCTTTTGGAGGATGGCAGCCTCCCTTGTGAGGT
  CCCTCACTTTGCTTCTCCAACAGCCTGTCGGGTAGTTGCCGCCTATCAGCTGTTTGATGCTACTGGACAA
  GGTCTAGATGCAGCTGACGCTGACTTCGTCTATGACTTGCTTTCTAGGCATGTGATCAGAGTCTTAGTAG
  GCGAGGGTGGTAGGGCTTCTGGTCCGCTCTCTCCTCAGAGGGCCCTCTGCCTCTTGGAACTCACCTTGGA
  ACATTGTCGTCGACTCTGCTGGAACCAGCACCACCACAAGGCTACCATAGCAGTGGAGAAGACTCGAGAT
  CACCTAGGAAATAGCAATGTAGCTCCCAGCCTCCAACTGTGCCAGATGGGGGTTGAGCTGCTGGAAGCTG
  TGGAAGAAAGACCAGGGGCAGTGGCCCAGCTCCTGATGAAGGCAGCTGCTATTCTGAACAGCAGTATGGA
  TGGGCCATCACCCCCGCTCCGGGCATTGTATGACAGCTGCCAGTTCTTCCTTTCAGGCCTGGAGAGAACC
  ATCAAGAGGCACTATGGAGTTGATGCCATATTGAATCTCTTTGCTTTTCTTGGGGGATACCACTCCCTTG
  TCCGGCACCTGCGAGAAGCCTCCGAAGCGTCCTCTAAGCAGCAGCAGAGCCTGCTCCAGATGCACTTCCA
  TACCCTCCACCTCTTCACTGGGATTGTGTATGACTTTGCCCAAGGCTGTCAGGTAGCTGAGCTTGCTCAG
  CTAGTGGACAGTTGCAGATCTGCTGTTGTCTGGATGTTGGAGGCCTTAGAGGGCCTGTCTGGTGGAGAGC
  TGGCTGACTACCTGGGCGTGACTGCCTCTTACACCAGCAACTTGGCCTACAGCTTCTTTAGTCAGAAGCT
  CTATGAGGAGGCTTGTGCCATCTCAGAGCCAGTCTGTCAGCACTTGGGCTTGGCTAAGTCAGGCGCTTGT
  CCCGAAGTGCCTCCTGAGAAGCTACATAGGTGCTTCCGGCTGCATGTGGAGAGTTTGAAGAAATGGGGTA
  AACAGGCCCAGGGCTGCAAGATGGTGACCTTATGGCTGGCAGCTCTGAAACCCTACGGTCTCCAACACAT
  GGTTGAGCCAGTCACTTTCTGGGTTCGGGTCAAAATGGATGCAGCCAAGGCAGGAGACAAGGAGCTACAA
  CTGCAGACTTTGCGAGACAGCCTAAGTTGCTGGGACCCAGAGATGCAGTCTCTGCTACTGAGGGAGGAGC
  TGCAGGCCTATAAGGCAGTACGGGCTGACACTGGGCAGGAACGCTTCAACGTCATCTGCGATCTGCTTGA
  GCTCAGCCCTGAGGACACAGCAGCTGGAGCCTGGGCACGAGCTACCCACCTGGTGGAATTGGCCCAAGTG
  CTGTGCTACCATAGCTTTGCCCAGCAGACAAACTGCTCTGCCTTGGATGCTATCCAGGAGGCCCTGCAGC
  TTCTGGAGTCCGTGAGTCCCGAGGCACAGGAGCAGGACCGCCTTCTAGATGACAAAGCACAGGCCCTGCT
  ATGGCTCTACATCTGCACCTTGGAGGCCAAAATGCAGGAAGGTATCGAGCGGGATCGGAGGGCCCAGGCC
  CCTAGTAACTTGGAGGAATTTGAAGTCAATGACCTGAACTATGAAGATAAACTGCAGGAAGATCGCTTTC
  TATACAGTAACATTGCCTTCAATCTGGCTGCTGATGCAGCGCAGTCCAGATGCCTGGACCAGGCCCTGAC
  TCTGTGGAAGGAAGTGCTCACCAAGGAGCCAGCCCCAGCTGTGCGGTGTCTCCAGCAGACGGCAGCCTCC
  CTGCAAATCCTGGTGGCCATCTATCAGTTGGTGGCAAAGCCCTTGCAGGCTCTGGAGACCCTCCTGCTCC
  TTCAGATTGTATCTAAGAGACTACAGGATCATGCAAAGGCAGTCAGCTCTTCCTGCCAGCTTACCCAGCT
  ACTCTTGAACCTTGGCTGTCCCAGCTATGCCCAGTTGTATCTGGAAGAGGCAGAGTCAAGCCTGAAGAAT
  CTGGACCAAGCGACTGACACGTGCCAGCTGCTTTCCCTGACCTGTGCTCTGCTTCGAAGCCAGCTCTGCT
  GGGCTTGCCAGAAGGTGACTGAGGGCGTTTCTCTGTTGCTTTCTGTCCTTCGAGATCCTGCCCTCCAGAA
  GTCATCCAAGGCTTGGTACTTGCTACGTGTCCAGGCCCTGCAAATTCTGGCTTTCTACCTCAGCCTCTCG
  TCCAACCTCCTCTCAAGTGCCCTACGAGAGGAGCTCTGGGATCAAGGCTGGCAGACCCCAGAGACAGCAC
  TGATAGATGCCCACAAGCTTCTGAGAAGCATCATCATCTTGCTGATGGGTGGCGATGTGCTCTCCGTTCA
  GAAAGCAGCTGTGGAGTCGCCCTTTCTGGATTACGGTGAGAATCTGGTGCAGAAATGGCAAGTTCTCACA
  GAGGTGCTGACCTGCTCGGAGAGGCTGGTCAGCCGCCTGGGCCGCCTGGGGAACGTGAGTGAAGCCAAGG
  CCTTTTGCTTGGAGGCCCTAAAACTTACCACCAAGCTGCAGATACCTCGCCAGTGTGCCCTGTTCCTTGT
  ACTGAAGGGTGAGCTGGAGCTGGCCCGGGGAGACACTGACCTCTGTCAGTCAGACTTGCAGCAGGTTCTG
  TTCTTGCTCGAGTCTTCCACAGAGTTTGGTGTGGTGACACAGCACCCAGACTCAGTGAAGAGGGTGCACA
  CGCAGAAAGGGACGCATAAGGCTCAAGTCCCCTGTTCCCCACCTCTACCAGAAGAGGAACCCTTCCTGAG
  AGGCCCTGCCCTTGAGCTGGTGGCCACTGTGCTCAAGGAGCCCGGTCCCATCCAACCCTCTGGAAACTCC
  TCCCCGGTCCTCAAGACCAAGGCATCACCCAAGCCTGGCTTCCTGTCTCACTTGCCCAGCTGTGATTGCT
  TACTGTGTGCCAGTCCTGCCCTCTCCACCGTCTGTCTGCGCTGGGTGTTGGTCACTGCAGGAGTGAGGCT
  CACCACAGGTCATAAAGCTCAGGGTCTGGATCTGCTGCAAGCTGTGCTGAAGGGTTGCCCTGCAGCCACC
  AAGCGCTTCACACAGAATCTTCAAACTTCTCTGAATCACAAAGCACCCCCCTCTTGTGTTCCAAGCCTCT
  TTGATGAGATCATGGCTCAAGTGTACACACACTTGGCATTAGAGTGCCTGAATCAGACATCTGAAAAGAG
  CCTGGGAAAGGTCCTGGCGTCAGGACTGAAGTTTGTGGCAACACGGATACAATACTTAGAGTTCTGGCAA
  GCCAGCCTGCTCTTGGTTCAGGCCCTTGCAAAGCTGGCCCGCTTCAGCTGCTGTACTAGCGAACTCTTTG
  CAAGCGCCTGGGGCTGGCAGCCACCATTAGTAAAAATCCCGCCAGTCCTTGAGCCTTCTAAGATTCGGAG
  ACAAAAATGCTCTGGACAGGGGCGCCAGCGGGTAGCCTCTGCCCCCTCACCCCTCCATAGCAGCTCTCAG
  GAAGGTCTGGAAGAAGGGCCACCTTGTACACCTAAGCCCCCAGGCCGTGCTAGGCAAGCCGGCCCTCGTG
  TCCCTTTCACCATATTTGAAGACATCCACCCCACAAAGAGCAAGCTTAAGGTTCCCTTGGCTCCCAGGGT
  CCACAGGCGGGCCCAGACTCGACTCAAGGTGAACTTCAGCGATGACAGCGACCTGGAAGACCTTGTCTCA
  GCTGAAACACAACTTGTGGAGGAACCTAAGAGGCGAGGGACTGCTTCCCGCACCCGGGGTCAGAGTAGGA
  AGGGCCCTAGCTTAAAGACAGATGCAGCGGTTGCCGTAGGTAGTACCCCTGGACATCCCAGTCTCAGTGG
  GAGGACTCGGAGGGCCAGGAAGGTGGCTTCAAGACATTGTGAAGGTGTCGGGGCCAGCCTGGGACCCGAA
  ATCATGAGAAGCATCCCTGAAGAGGAGCTGATGGACAGCCAGCTAGAAAAGAGCTTTGAGATTCTCAGGG
  CTTCTGATGGGGAAGACTCAGCCTCAGGCGAGAAGGCAGCAGCTGCAGAGCCTGGTCTACCCATAGGAGA
  GCGTGAGGTGTTGAGACGGGACTCCAGCAAGGCAGAGCGACCTGTCTTGTACCCTGATACAGAGGCCGAT
  AGTGACCTTAGTCCTTGGCTCCCACCCTTCTCAATTCCTGCACCCATTGATCTTTCTACCCTGGATTCCA
  TCGCTGACTCATTGAGCATTGCTTTTCGTGGTATTAGTCACTGCCCTCCAAGTGGGCTCTACGCTCACCT
  CTGCCGCTTTTTGGCCTTGTGTCTGGGCCACCGGGATCCCTATGCCACTGCGTTCCTTGTAGCAGAGTCT
  GTCTCCATCACCTGCCGTCACCAGCTACTCACCCATTTGCACCGACAGCTCAGCAAGGCCCAGAAGCAGC
  AAGGCTCACCTGAACTAGCAGAGCATCTACAGAGGCTGGACTTGAAGGAGAGGCCCGGAGGTGTCCCACT
  GGCCCGCGTGCAGCACCTCTTTTCCTTCAGGGCTTTGGGATCTGGCTGCTTCCCCCAGGCAGAGAAGGAC
  AGTTTCCAGAAGCGCCTGGCTCTCATCCCCAGTGGGGTGACTGTCTGCGTGTTGGCCCTGGCCACCCTCC
  AGCCTGGAACCTTGAGCAACACTCTCTTACTGACCCGCCTGGAAAAGGACAACCCCCCAATTACTGTGAA
  GATCCCCACTGCCCAAAGTAAGTTCCCACTAAGTGCGGTGCTGAAAGAGTTCGATGCCATCCAGAAGGAC
  CAGAAAGAGAACAGCGGCTGTACTGAGAAACGAGCGTGGTGGACGGGCCGGCTAGCACTAGACCAAAGGA
  TGGAGGAACTCATTACCTCCCTAGAGGAGCATGTGCTGGGCTGCTGGAGGGGGCTGCTGCTGCCGTGTAG
  CACCGACCCTGGCCTTTCCCAGGAGGCCTCCCAACTACAAGAGTTGCTGCACGAATGTGGCTGGGAATAT
  CCTGATTACACTCTCCTAAAAGTCATTCTTAGTGGGGCCAGAATCCTCTCCAGCCAGGATGTTCAGGCCT
  TGGCATGTGGGCTGTGCCCAGCCCAACCTGAGCGAGCCCAAGTGCTCCTGAGCGAGGCAGTAGGACGGGT
  ACAGAGCCAGGCAGCCCCTCCGAGTCAACATCTTGTACTGGTGTTAGACAAGGACCTGCAGAAGCTGCCG
  TGGGAAAGCATGCCATGCCTCCGAGCACTGCCGGTCACCCGGCTGCCCTCCCTCCGCTTCCTGCTCAGCT
  ACTCAATCGCTAAGGAGGCTGGGGCCTCATCAGTGCTGAGCCAAGGTGTTGATCCACGCAATACCTTCTA
  TGTGCTAAACCCTCACAGTAACCTGTCGAGCACGGAGGAGAGATTTCGAGCCAGTTTTAGCAGTGAAGCC
  GGATGGAAAGGCGTGATTGGGGAAGTGCCCAGCCTTGAGCAGGTGCAGGCTGCCCTGACAGAGCATGATT
  TATATATCTATGCAGGACATGGAGCTGGTGCCCGCTTCCTTGATGGGCAGGCTGTCCTGCGGCTGAACTG
  CCGGGCAGTGGCCCTACTGTTCGGCTGCAGCAGTGCAGCCCTCGCAGTGCATGGAAACCTGGAGGGAGCT
  GGTATTGTGCTCAAGTACATCATGGCTGGCTGCCCCTTGTTCCTAGGTAATCTCTGGGATGTGACTGACC
  GTGATATTGACCGTTACACAGAGGCTTTGCTGCAGGGCTGGCTGGGAGCAGGCCCAGGGGCTCCCCTTCT
  CCACTATGTCAGCCAAGCCCGCCAGGCCCCACGACTCAAGTATCTTATTGGTGCTGCACCTGTAGCCTAC
  GGCCTACCTGTCTCTCTACAAACATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217737 representing NM_001170602
  Red=Cloning site Green=Tags(s)
  
  MRNFKGVKFATLLCCKRETQELLPDLKEFLSRSRTDSVSSRTDAERRQVCDTILRACTQQLTAKLDGPGH
  LRSILDLAELACDGYLLSTPQRPPLYLERILFILLRNGLTQGNPDAVLRLAQPLHACLVQNSREAAPQDY
  EAVTRGTFSLLWKGAEALSERRAAFSARLKALSFLVLLEDGSLPCEVPHFASPTACRVVAAYQLFDATGQ
  GLDAADADFVYDLLSRHVIRVLVGEGGRASGPLSPQRALCLLELTLEHCRRLCWNQHHHKATIAVEKTRD
  HLGNSNVAPSLQLCQMGVELLEAVEERPGAVAQLLMKAAAILNSSMDGPSPPLRALYDSCQFFLSGLERT
  IKRHYGVDAILNLFAFLGGYHSLVRHLREASEASSKQQQSLLQMHFHTLHLFTGIVYDFAQGCQVAELAQ
  LVDSCRSAVVWMLEALEGLSGGELADYLGVTASYTSNLAYSFFSQKLYEEACAISEPVCQHLGLAKSGAC
  PEVPPEKLHRCFRLHVESLKKWGKQAQGCKMVTLWLAALKPYGLQHMVEPVTFWVRVKMDAAKAGDKELQ
  LQTLRDSLSCWDPEMQSLLLREELQAYKAVRADTGQERFNVICDLLELSPEDTAAGAWARATHLVELAQV
  LCYHSFAQQTNCSALDAIQEALQLLESVSPEAQEQDRLLDDKAQALLWLYICTLEAKMQEGIERDRRAQA
  PSNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSRCLDQALTLWKEVLTKEPAPAVRCLQQTAAS
  LQILVAIYQLVAKPLQALETLLLLQIVSKRLQDHAKAVSSSCQLTQLLLNLGCPSYAQLYLEEAESSLKN
  LDQATDTCQLLSLTCALLRSQLCWACQKVTEGVSLLLSVLRDPALQKSSKAWYLLRVQALQILAFYLSLS
  SNLLSSALREELWDQGWQTPETALIDAHKLLRSIIILLMGGDVLSVQKAAVESPFLDYGENLVQKWQVLT
  EVLTCSERLVSRLGRLGNVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARGDTDLCQSDLQQVL
  FLLESSTEFGVVTQHPDSVKRVHTQKGTHKAQVPCSPPLPEEEPFLRGPALELVATVLKEPGPIQPSGNS
  SPVLKTKASPKPGFLSHLPSCDCLLCASPALSTVCLRWVLVTAGVRLTTGHKAQGLDLLQAVLKGCPAAT
  KRFTQNLQTSLNHKAPPSCVPSLFDEIMAQVYTHLALECLNQTSEKSLGKVLASGLKFVATRIQYLEFWQ
  ASLLLVQALAKLARFSCCTSELFASAWGWQPPLVKIPPVLEPSKIRRQKCSGQGRQRVASAPSPLHSSSQ
  EGLEEGPPCTPKPPGRARQAGPRVPFTIFEDIHPTKSKLKVPLAPRVHRRAQTRLKVNFSDDSDLEDLVS
  AETQLVEEPKRRGTASRTRGQSRKGPSLKTDAAVAVGSTPGHPSLSGRTRRARKVASRHCEGVGASLGPE
  IMRSIPEEELMDSQLEKSFEILRASDGEDSASGEKAAAAEPGLPIGEREVLRRDSSKAERPVLYPDTEAD
  SDLSPWLPPFSIPAPIDLSTLDSIADSLSIAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVAES
  VSITCRHQLLTHLHRQLSKAQKQQGSPELAEHLQRLDLKERPGGVPLARVQHLFSFRALGSGCFPQAEKD
  SFQKRLALIPSGVTVCVLALATLQPGTLSNTLLLTRLEKDNPPITVKIPTAQSKFPLSAVLKEFDAIQKD
  QKENSGCTEKRAWWTGRLALDQRMEELITSLEEHVLGCWRGLLLPCSTDPGLSQEASQLQELLHECGWEY
  PDYTLLKVILSGARILSSQDVQALACGLCPAQPERAQVLLSEAVGRVQSQAAPPSQHLVLVLDKDLQKLP
  WESMPCLRALPVTRLPSLRFLLSYSIAKEAGASSVLSQGVDPRNTFYVLNPHSNLSSTEERFRASFSSEA
  GWKGVIGEVPSLEQVQAALTEHDLYIYAGHGAGARFLDGQAVLRLNCRAVALLFGCSSAALAVHGNLEGA
  GIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLHYVSQARQAPRLKYLIGAAPVAY
  GLPVSLQTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170602
• ORF Size: 6327 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001170602.1, NP_001164073.1
• RefSeq Size: 6835
• RefSeq ORF: 6330
• Locus ID: 315330
• MW: 231.6 kDa