STAT3 (NM_213662) Human Untagged Clone

CAT#: SC100105

STAT3 (untagged)-Human signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STAT3
• Synonyms: ADMIO; ADMIO1; APRF; HIES
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100105 sequence for NM_213662 edited (data generated by NextGen Sequencing)
  ATGGCCCAATGGAATCAGCTACAGCAGCTTGACACACGGTACCTGGAGCAGCTCCATCAG
  CTCTACAGTGACAGCTTCCCAATGGAGCTGCGGCAGTTTCTGGCCCCTTGGATTGAGAGT
  CAAGATTGGGCATATGCGGCCAGCAAAGAATCACATGCCACTTTGGTGTTTCATAATCTC
  CTGGGAGAGATTGACCAGCAGTATAGCCGCTTCCTGCAAGAGTCGAATGTTCTCTATCAG
  CACAATCTACGAAGAATCAAGCAGTTTCTTCAGAGCAGGTATCTTGAGAAGCCAATGGAG
  ATTGCCCGGATTGTGGCCCGGTGCCTGTGGGAAGAATCACGCCTTCTACAGACTGCAGCC
  ACTGCGGCCCAGCAAGGGGGCCAGGCCAACCACCCCACAGCAGCCGTGGTGACGGAGAAG
  CAGCAGATGCTGGAGCAGCACCTTCAGGATGTCCGGAAGAGAGTGCAGGATCTAGAACAG
  AAAATGAAAGTGGTAGAGAATCTCCAGGATGACTTTGATTTCAACTATAAAACCCTCAAG
  AGTCAAGGAGACATGCAAGATCTGAATGGAAACAACCAGTCAGTGACCAGGCAGAAGATG
  CAGCAGCTGGAACAGATGCTCACTGCGCTGGACCAGATGCGGAGAAGCATCGTGAGTGAG
  CTGGCGGGGCTTTTGTCAGCGATGGAGTACGTGCAGAAAACTCTCACGGACGAGGAGCTG
  GCTGACTGGAAGAGGCGGCAACAGATTGCCTGCATTGGAGGCCCGCCCAACATCTGCCTA
  GATCGGCTAGAAAACTGGATAACGTCATTAGCAGAATCTCAACTTCAGACCCGTCAACAA
  ATTAAGAAACTGGAGGAGTTGCAGCAAAAAGTTTCCTACAAAGGGGACCCCATTGTACAG
  CACCGGCCGATGCTGGAGGAGAGAATCGTGGAGCTGTTTAGAAACTTAATGAAAAGTGCC
  TTTGTGGTGGAGCGGCAGCCCTGCATGCCCATGCATCCTGACCGGCCCCTCGTCATCAAG
  ACCGGCGTCCAGTTCACTACTAAAGTCAGGTTGCTGGTCAAATTCCCTGAGTTGAATTAT
  CAGCTTAAAATTAAAGTGTGCATTGACAAAGACTCTGGGGACGTTGCAGCTCTCAGAGGA
  TCCCGGAAATTTAACATTCTGGGCACAAACACAAAAGTGATGAACATGGAAGAATCCAAC
  AACGGCAGCCTCTCTGCAGAATTCAAACACTTGACCCTGAGGGAGCAGAGATGTGGGAAT
  GGGGGCCGAGCCAATTGTGATGCTTCCCTGATTGTGACTGAGGAGCTGCACCTGATCACC
  TTTGAGACCGAGGTGTATCACCAAGGCCTCAAGATTGACCTAGAGACCCACTCCTTGCCA
  GTTGTGGTGATCTCCAACATCTGTCAGATGCCAAATGCCTGGGCGTCCATCCTGTGGTAC
  AACATGCTGACCAACAATCCCAAGAATGTAAACTTTTTTACCAAGCCCCCAATTGGAACC
  TGGGATCAAGTGGCCGAGGTCCTGAGCTGGCAGTTCTCCTCCACCACCAAGCGAGGACTG
  AGCATCGAGCAGCTGACTACACTGGCAGAGAAACTCTTGGGACCTGGTGTGAATTATTCA
  GGGTGTCAGATCACATGGGCTAAATTTTGCAAAGAAAACATGGCTGGCAAGGGCTTCTCC
  TTCTGGGTCTGGCTGGACAATATCATTGACCTTGTGAAAAAGTACATCCTGGCCCTTTGG
  AACGAAGGGTACATCATGGGCTTTATCAGTAAGGAGCGGGAGCGGGCCATCTTGAGCACT
  AAGCCTCCAGGCACCTTCCTGCTAAGATTCAGTGAAAGCAGCAAAGAAGGAGGCGTCACT
  TTCACTTGGGTGGAGAAGGACATCAGCGGTAAGACCCAGATCCAGTCCGTGGAACCATAC
  ACAAAGCAGCAGCTGAACAACATGTCATTTGCTGAAATCATCATGGGCTATAAGATCATG
  GATGCTACCAATATCCTGGTGTCTCCACTGGTCTATCTCTATCCTGACATTCCCAAGGAG
  GAGGCATTCGGAAAGTATTGTCGGCCAGAGAGCCAGGAGCATCCTGAAGCTGACCCAGGT
  AGCGCTGCCCCATACCTGAAGACCAAGTTTATCTGTGTGACACCATTCATTGATGCAGTT
  TGGAAATAA
  
  Clone variation with respect to NM_213662.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_213662 unedited
  GTCTTGGATTTGTATCCGACTTCTATAGGCGGCCGCGCAATTCGCACGAGGTGGACAGGC
  ACCCCGGCTTGGCGCTGTCTCTCCCCCTCGGCTCGGAGAGGCCCTTCGGCCTGAGGGAGC
  CTCGCCGCCCGTCCCCGGCACACGCGCAGCCCCGGCCTCTCGGCCTCTGCCGGAGAAACA
  GTTGGGACCCCTGATTTTAGCAGGATGGCCCATTGGAATCAGCTACAGCAGCTTGACACA
  CGGTACCTGGAGCAGCTCCATCAGCTCTACAGTGACAGCTTCCCAATGGAGCTGCGGCAG
  TTTCTGGCCCCTTGGATTGAGAGTCAAGATTGGGCATATGCGGCCAGCAAAGAATCACAT
  GCCACTTTGGTGTTTCATAATCTCCTGGGAGAGATTGACCAGCAGTATAGCCGCTTCCTG
  CAAGAGTCGAATGTTCTCTATCAGCACAATCTACGAAGAATCAAGCAGTTTCTTCAGAGC
  AGGTATCTTGAGAAGCCAATGGAGATTGCCCGGATTGTGGCCCGGTGCCTGTGGGAAGAA
  TCACGCCTTCTACAGACTGCAGCCACTGCGGCCCAGCAAGGGGGCCAGGCCAACCACCCC
  ACAGCAGCCGTGGTGACGGAGAAGCAGCAGATGCTGGAGCAGCACCTTCAGGATGTCCGG
  AAGAGAGTGCAGGATCTAGAACAGANAATGAAAGTGGTAGAGAATCTCCAGGATGACTTT
  GATTTCAACTATAANACCCTCAAGAGTCAAGGAGACATGCAAGATCTGAATGGGANACAC
  CAGTCAGTGACCAGGCAGAAGATGCAGCAGCTGGAACAGATGCTCACTGCGCTGGACCAG
  ATGCGGAGAAGCATCGTGAGTGAGCTGGCGGNGCTTTGTCAGCGATGGAGTACGTGCAGA
  AAACTCTCACGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_213662 unedited
  GCTATGAACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAAACAGAG
  TCTCAGACTGTCGCCCAGGATGGAGTGCAGTGTGCAGTGGTGCAATTTTGGCTCACTGCA
  ACCTCCGCCTCTCAGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTA
  CAGGCGCCCACCACCACACCCAGCTAATTTTTTGTACTTTTAGTAGAGACGGGGTTTCAC
  CGTGTTAGCCAGGATGGTCTTGATCTCCTGACCTTATGATCCGCCTCGGCCTCCCAAAGT
  GCTGGGATTACAGGCGTGAGCCACCATGCCCGGCTGCTTAAGTTTCTTAAATACAGAAAG
  GCTATGCTGATACAGTGTTTTTTGCCCTTTAATTGGTATTATTTCTTAATTTAAAAAGAA
  ACCTAGGGCTTAGATAGTCCTATCTTCTATTTGGATGTCAGCAAGGTTAAAAAGTGCAAT
  GCCAGGAGTATGTACCTATAGGTGGCCTGTGGCATTTGCTTACAGAAACAGGCAGAAGGA
  TGCCGCAGGCACCAGGAGGCACTTGTCTAAGAACACAACCACAATAACAAAAAGCTGCTG
  ACAAAGGAGGGCAGGGGAACAAAACAACACAAGACATTTCCTTTTTCTCCCTCTAGCCAC
  CCCCCCGCCACATCCCCTGATCATGGGTCTCAGAGAACACATCCTTATTTGCATTTAGAT
  ANAAGCAGATCACCCACATTCACTCATTTCTCTATTTTTAAAGTGCCCACATTGCTCAAG
  ATAGCACAAGTAGGAGATTAAAAAATCTGGACCCCAAGTTAGTATTTCAAAGATCTGGGT
  TTGCTGGGGAGGGGCGCAAATGCAGTAGGCCCTCACCGATCTTTTGAACTCCGTCTCACT
  CTTCAGGGGAGTACGCCCCCAGGCATCCTGCAAGTGGGACTAACGCCTCTGTAGGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_213662
• Insert Size: 3570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_213662.1, NP_998827.1
• RefSeq Size: 4819 bp
• RefSeq ORF: 2169 bp
• Locus ID: 6774
• Cytogenetics: 17q21.2
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Chemokine signaling pathway, Jak-STAT signaling pathway, Pancreatic cancer, Pathways in cancer
• Gene Summary: 'The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. [provided by RefSeq, Aug 2020]'
  Transcript Variant: This variant (3), also called STAT3B or STAT3beta, uses an alternate splice site in the 3' coding region, resulting in an early stop codon, compared to variant 1. The encoded isoform (3) has a shorter and distinct C-terminus, compared to isoform 1.