RBJ (DNAJC27) (NM_016544) Human Untagged Clone

CAT#: SC100369

DNAJC27 (untagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 27 (DNAJC27), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNAJC27
• Synonyms: RabJS; RBJ
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100369 sequence for NM_016544 edited (data generated by NextGen Sequencing)
  ATGGAGGCCAACATGCCGAAGCGGAAGGAGCCTGGCAGGTCTCTCCGCATCAAAGTCATC
  TCCATGGGCAACGCCGAAGTGGGGAAAAGCTGTATTATAAAGCGATACTGTGAGAAAAGA
  TTCGTGTCTAAATACCTGGCAACAATTGGAATTGACTATGGAGTCACAAAGGTACACGTC
  AGAGACAGAGAAATCAAAGTTAACATCTTTGATATGGCTGGACATCCCTTCTTCTATGAG
  GTTCGAAATGAGTTTTACAAGGACACACAGGGTGTGATACTGGTCTATGATGTTGGGCAG
  AAAGACTCCTTTGACGCCCTTGATGCGTGGCTGGCAGAAATGAAGCAAGAGCTTGGACCT
  CATGGAAACATGGAAAATATTATATTTGTAGTTTGTGCCAACAAGATTGATTGTACCAAA
  CATCGCTGTGTAGATGAAAGTGAAGGACGTCTTTGGGCTGAAAGCAAAGGGTTCCTGTAC
  TTTGAAACTTCAGCACAAACTGGAGAAGGCATTAATGAGATGTTCCAGACCTTTTATATA
  TCCATAGTTGATTTATGTGAAAATGGCGGGAAACGCCCTACCACCAATAGCAGTGCTAGT
  TTCACCAAAGAACAAGCAGATGCCATTCGCAGAATTCGAAATAGTAAAGACAGTTGGGAC
  ATGCTGGGAGTCAAACCTGGGGCCTCAAGGGATGAAGTCAATAAAGCGTATCGGAAACTT
  GCTGTGCTTCTTCACCCTGACAAATGTGTAGCACCTGGCAGTGAAGATGCCTTCAAAGCA
  GTTGTGAATGCTCGGACAGCCCTCCTGAAAAACATCAAGTAG
  
  Clone variation with respect to NM_016544.2
  33 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016544 unedited
  CTCTTGTATACGACTCCTATAGGCGGCCGCGAAATTCGCACGAGGGAAAAGACCGAGGCG
  GGTGGNCCCCGNATAGAGCCAGGGCCATGGAGGCCAACATGCCGAAGCGGAAGGAGCCTG
  GCAGGTCTCTCCGCATCAAAGTCATCTCCATGGGCAACGCCNAAGTGGGGAAAAGCTGTA
  TTATAAAGCGATACTGTGAGAAAAGATTCGTGTCTAAATACCTGGCAACAATTGGAATTG
  ACTATGGAGTCACAAAGGTACACGTCAGAAACAGAGAAATCAAAGTTAACATCTTTGATA
  TGGCTGGACATCCCTTCTTCTATGAGGTTCGAAATGAGTTTTACAAGGACACACAGGGTG
  TGATACTGGTCTATGATGTTGGGCAGAAAGACTCCTTTGACGCCCTTGATGCGTGGCTGG
  CAGAAATGAAGCAAGAGCTTGGACCTCATGGAAACATGGAAAATATTATATTTGTAGTTT
  GTGCCAACAAGATTGATTGTACCAAACATCGCTGTGTAGATGAAAGTGAAGGACGTCTTT
  GGGCTGAAGCAAAGGGTTCCTGTACTTTGAAACTTCAGCACAAACTGGAGAAGGCATTAA
  TGAGATGTTCCAGAACTTTTATATATCCATAGTTGATTTATGTGAAAATGGCGGGAAACG
  CCCTACCCCAATAGCAGTGCTAGTTTCACCAAAAACAAGCAGATGCCATTCGCAGAATTC
  GAAATAGTANAGACAGTTGGGACATGCCTTGGAAGTCAAACCTGGGCCTCAAGGAATATG
  AGTCATTNAAGCGTATCGAAACTTTTGCTGTGCTTCTTCACCTGACAAATGTGTAGACCT
  TTTGCAANGGAAAATGCTTCAAAAAAAAATTTGGGATTTGCTCGAGACAGNCCTTCTTGA
  AACATAGGTTGANAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016544 unedited
  AGCTTGTACGCGGCCGCTTCTANGATCGAGTTTTTTTTTTTTTTTTTTAAAAAACTGATT
  CAGAGCTTGCAGTGGCTGCTTACTTACTCTGGCTATCTTTCAGAAATTTAGCTGCCTTAT
  CTATAGCTCATTTAACTAACAGTGAATTAGGTACTAGTTTATAGGTATAAAGAAATAGTT
  ACTTAGTTGAGAATTCAATATATGAGAACATACTAGAGTAAGTTTCCTGAGGTAAAAGTA
  TGCCTTAAAGAATGATGTGCAATCTATAAAACTGTAGCATTTTCTCTTGGGTAGCAACAC
  AATGACCCCCAAGTCTGGATTTTTAAATTAACAGGCTAAGAAAACTAAGGACACATTGAT
  ATAGAGAAATAAAATAACAATGCTGAAGTCAAAGAAATATTGACAGCTGAGATATCAATT
  CAAAAGGAGGCATGAAGGGGAACAGAGGAACAGAAGGAGGCTGTATTTTAAAGTGCTTAG
  GTAGGTTAGAAGATGAATTTGAAATGCCTCCAGGGATTTCTAACAAGTTTAAAAAGTGTT
  ACTTCTTGGTGACTCAGATGTTGAAGACATGGCTTTTCTTTATAATAGCTATTCAAACTT
  TTTATTCTTTTTTGAAAAACAAGATTCTCTATAAAAACGTTTTAGCCATTGGTATTATTC
  TGCTAGCTGTTACTCTCACTAATGTATTTTGAAATCCCTACTGGCTGAAGAATTTTTAAT
  AAGGTTGAACTACAAAGCACACATTTGCACCAAGAATTTTAATTAATGCATAGATGTCTG
  TGAAAATCTGCATGAAGTTATGGCAAAAAAACCTTTTCCATGGAGATCATGCTTTAATTC
  CTAATATTTCTGCTGGAGGGATATTTGAAATCCGAAAAATTCTTGGGACCCTGGAGCTTA
  ACTTGGCTAATTTTATTCCCTTTTACGTAAATGAAGTGGTATATGGCGCATTCCTTACAT
  TAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016544
• ORF Size: 822 bp
• Insert Size: 4700
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_016544.1, NP_057628.1
• RefSeq Size: 4869
• RefSeq ORF: 822
• Locus ID: 51277
• Domains: DnaJ, ras, RAN, RAS, RHO, RAB
• Gene Summary: GTPase which can activate the MEK/ERK pathway and induce cell transformation when overexpressed. May act as a nuclear scaffold for MAPK1, probably by association with MAPK1 nuclear export signal leading to enhanced ERK1/ERK2 signaling. [UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.