RBJ (DNAJC27) (NM_016544) Human Tagged ORF Clone

CAT#: RC205500

DNAJC27 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 27 (DNAJC27), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY413917) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205500 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DNAJC27 protein (Cat# TP305500). The protein was produced from HEK293T cells transfected with DNAJC27 cDNA clone (Cat# RC205500) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DNAJC27
• Synonyms: RabJS; RBJ
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205500 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCAACATGCCGAAGCGGAAGGAGCCTGGCAGGTCTCTCCGCATCAAAGTCATCTCCATGGGCA
  ACGCCGAAGTGGGGAAAAGCTGTATTATAAAGCGATACTGTGAGAAAAGATTCGTGTCTAAATACCTGGC
  AACAATTGGAATTGACTATGGAGTCACAAAGGTACACGTCAGAGACAGAGAAATCAAAGTTAACATCTTT
  GATATGGCTGGACATCCCTTCTTCTATGAGGTTCGAAATGAGTTTTACAAGGACACACAGGGTGTGATAC
  TGGTCTATGATGTTGGGCAGAAAGACTCCTTTGACGCCCTTGATGCGTGGCTGGCAGAAATGAAGCAAGA
  GCTTGGACCTCATGGAAACATGGAAAATATTATATTTGTAGTTTGTGCCAACAAGATTGATTGTACCAAA
  CATCGCTGTGTAGATGAAAGTGAAGGACGTCTTTGGGCTGAAAGCAAAGGGTTCCTGTACTTTGAAACTT
  CAGCACAAACTGGAGAAGGCATTAATGAGATGTTCCAGACCTTTTATATATCCATAGTTGATTTATGTGA
  AAATGGCGGGAAACGCCCTACCACCAATAGCAGTGCTAGTTTCACCAAAGAACAAGCAGATGCCATTCGC
  AGAATTCGAAATAGTAAAGACAGTTGGGACATGCTGGGAGTCAAACCTGGGGCCTCAAGGGATGAAGTCA
  ATAAAGCGTATCGGAAACTTGCTGTGCTTCTTCACCCTGACAAATGTGTAGCACCTGGCAGTGAAGATGC
  CTTCAAAGCAGTTGTGAATGCTCGGACAGCCCTCCTGAAAAACATCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205500 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEANMPKRKEPGRSLRIKVISMGNAEVGKSCIIKRYCEKRFVSKYLATIGIDYGVTKVHVRDREIKVNIF
  DMAGHPFFYEVRNEFYKDTQGVILVYDVGQKDSFDALDAWLAEMKQELGPHGNMENIIFVVCANKIDCTK
  HRCVDESEGRLWAESKGFLYFETSAQTGEGINEMFQTFYISIVDLCENGGKRPTTNSSASFTKEQADAIR
  RIRNSKDSWDMLGVKPGASRDEVNKAYRKLAVLLHPDKCVAPGSEDAFKAVVNARTALLKNIK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016544
• ORF Size: 819 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016544.1, NM_016544.2, NP_057628.1
• RefSeq Size: 5008 bp
• RefSeq ORF: 822 bp
• Locus ID: 51277
• Domains: DnaJ, ras, RAN, RAS, RHO, RAB
• MW: 30.9 kDa
• Gene Summary: GTPase which can activate the MEK/ERK pathway and induce cell transformation when overexpressed. May act as a nuclear scaffold for MAPK1, probably by association with MAPK1 nuclear export signal leading to enhanced ERK1/ERK2 signaling. [UniProtKB/Swiss-Prot Function]