RALGDS (NM_006266) Human Untagged Clone

CAT#: SC100409

RALGDS (untagged)-Human ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 1


  "NM_006266" in other vectors (4)

Reconstitution Protocol

USD 1,850.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol RALGDS
Synonyms RalGEF; RGDS; RGF
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_006266, the custom clone sequence may differ by one or more nucleotides


ATGGTGCAGCGCATGTGGGCCGAGGCGGCCGGGCCTGCTGGCGGCGCCGAGCCGCTGTTTCCGGGCTCCC
GGCGGAGCCGCAGCGTGTGGGACGCCGTGCGCCTGGAGGTGGGCGTCCCCGACAGCTGCCCGGTGGTGCT
GCACAGCTTCACGCAGCTAGACCCCGACCTGCCGCGCCCGGAGAGCTCCACGCAGGAGATCGGTGAGGAG
CTGATCAACGGAGTCATCTACTCCATCTCCCTGCGCAAGGTGCAGCTGCACCACGGAGGCAACAAGGGGC
AGCGCTGGCTCGGGTATGAGAATGAGTCGGCCCTGAACCTTTATGAGACTTGCAAGGTGCGGACCGTGAA
GGCTGGCACGCTGGAGAAGCTGGTGGAGCACCTGGTGCCAGCCTTCCAGGGCAGCGACCTCTCCTACGTC
ACCATCTTCCTGTGTACCTATAGAGCCTTCACCACCACCCAACAGGTCCTGGACCTGCTGTTCAAAAGGT
ACGGTAGATGTGACGCCCTCACGGCCTCCTCTAGATACGGCTGCATCCTCCCCTATTCCGACGAGGATGG
TGGACCCCAGGACCAACTTAAAAATGCCATCTCCTCCATCCTGGGCACCTGGCTGGACCAGTACTCGGAG
GATTTCTGTCAACCTCCGGACTTTCCCTGCCTCAAGCAGCTGGTGGCCTACGTGCAGCTCAACATGCCAG
GCTCAGACCTGGAGCGCCGTGCCCACCTTCTCCTGGCCCAGCTGGAGCACTCGGAACCCATTGAGGCAGA
GCCTGAGGCTCTGTCACCAGTGCCAGCTCTAAAACCAACTCCAGAGCTCGAGCTAGCTCTAACACCAGCT
CGAGCACCCAGCCCAGTGCCGGCTCCAGCCCCGGAGCCAGAGCCAGCTCCAACACCAGCTCCAGGTTCAG
AGCTAGAAGTAGCTCCAGCACCAGCTCCGGAGCTCCAGCAGGCTCCAGAGCCAGCTGTGGGACTAGAATC
GGCTCCAGCGCCAGCTCTGGAACTAGAGCCAGCTCCAGAACAGGATCCAGCTCCCTCACAAACTCTAGAG
CTGGAGCCAGCTCCAGCACCAGTTCCATCATTACAGCCTTCCTGGCCTTCACCTGTGGTTGCAGAGAACG
GGCTGAGTGAGGAGAAGCCTCACCTCTTGGTGTTCCCTCCAGATCTGGTGGCAGAGCAGTTTACACTGAT
GGATGCGGAACTGTTCAAGAAGGTGGTGCCCTACCACTGCCTGGGCTCCATCTGGTCCCAGCGGGACAAG
AAGGGCAAGGAGCACCTGGCGCCCACCATCCGCGCCACTGTCACCCAGTTCAACAGTGTGGCCAACTGTG
TCATCACCACCTGCCTCGGGAACCGAAGCACGAAAGCCCCAGACAGGGCCAGGGTGGTGGAGCACTGGAT
CGAGGTGGCCAGGGAGTGCCGGATCCTCAAGAACTTCTCGTCACTGTATGCCATCCTCTCTGCCCTGCAG
AGCAACTCCATCCACCGTCTGAAGAAGACGTGGGAAGACGTTTCCAGGGACAGTTTCCGGATCTTTCAGA
AGCTGTCAGAGATCTTCTCAGATGAGAACAACTACTCATTGAGCCGGGAGCTGCTCATCAAGGAGGGCAC
CTCCAAGTTTGCCACCCTGGAGATGAACCCCAAGAGAGCCCAGAAACGGCCGAAGGAGACGGGCATCATC
CAGGGCACCGTTCCCTACCTGGGCACGTTCCTCACCGACCTGGTGATGCTGGACACTGCCATGAAGGACT
ATCTGTATGGCAGACTCATCAACTTTGAGAAGAGGAGGAAGGAGTTCGAGGTGATCGCCCAGATCAAGCT
GCTGCAGTCGGCCTGCAACAACTACAGCATCGCGCCAGATGAGCAATTTGGGGCCTGGTTCCGGGCCGTG
GAGCGGCTCAGCGAGACTGAGAGCTACAACCTGTCGTGCGAGCTGGAGCCCCCATCCGAGTCAGCCAGCA
ACACCCTCAGGACCAAGAAGAACACAGCCATTGTCAAGCGCTGGAGCGACCGCCAGGCCCCCAGCACTGA
GCTCAGTACCAGTGGCAGCTCCCACTCCAAGTCCTGTGACCAGCTCAGGTGTGGCCCCTACCTCAGCAGC
GGGGACATCGCTGACGCGCTCAGCGTGCACTCGGCCGGCTCCTCTAGCTCCGACGTGGAGGAGATCAACA
TCAGCTTCGTCCCGGAGTCTCCTGATGGCCAGGAAAAGAAGTTCTGGGAATCAGCCTCACAGTCATCCCC
GGAGACCTCCGGCATCAGCTCAGCCTCCAGCAGCACCTCGTCCTCCTCAGCCTCCACCACGCCCGTGGCT
GCCACACGCACCCACAAGCGCTCTGTCTCAGGGCTCTGCAACTCCAGCTCCGCGCTGCCGCTCTACAACC
AGCAGGTGGGCGACTGCTGTATCATCCGCGTCAGCCTGGACGTGGACAATGGCAACATGTACAAGAGCAT
CCTGGTGACCAGCCAAGATAAGGCTCCGGCTGTAATCCGCAAGGCCATGGACAAACACAACCTGGAGGAG
GAGGAGCCGGAGGACTATGAGCTGCTGCAGATTCTCTCAGATGACCGGAAGCTGAAGATCCCTGAAAACG
CCAACGTCTTCTATGCCATGAACTCTACCGCCAACTATGACTTTGTCCTCAAGAAGCGGACCTTCACCAA
GGGAGTGAAGGTCAAGCACGGAGCCAGCTCCACCCTCCCTCGCATGAAGCAGAAAGGACTCAAGATTGCC
AAGGGCATCTTCTGA


>OriGene 5' read for NM_006266 unedited
AGGCGGCCGCGCAATTCGCACGAGGGCCGCGCGGGCCGCGCCGCCGCCCCCGCCGCGCGC
CGGGCCGGCCCCGCGCCCGCGCTGCGAGCCTTCCATGGTGCAGCGCATGTGGGCCGAGGC
GGCCGGGCCTGCTGGCGGCGCCGAGCCGCTGTTTCCGGGCTCCCGGCGGAGCCGCAGCGT
GTGGGACGCCGTGCGCCTGGAGGTGGGCGTCCCCGACAGCTGCCCGGTGGTGCTGCACAG
CTTCACGCAGCTAGACCCCGACCTGCCGCGCCCGGAGAGCTCCACGCAGGAGATCGGTGA
GGAGCTGATCAACGGAGTCATCTACTCCATCTCCCTGCGCAAGGTGCAGCTGCACCACGG
AGGCAACAAGGGGCAGCGCTGGCTCGGGTATGAGAATGAGTCGGCCCTGAACCTTTATGA
GACTTGCAAGGTGCGGACCGTGAAGGCTGGCACGCTGGAGAAGCTGGTGGAGCACCTGGT
GCCAGCCTTCCAGGGCAGCGACCTCTCCTACGTCACCATCTTCCTGTGTACCTATAGAGC
CTTCACCACCACCCAACAGGTCCTGGACCTGCTGTTCAAAAGATACGGCTGCATCCTCCC
CTATTCCGACGAGGATGGTGGACCCCAGGACCAACTTAANAATGCCATCTCCTNCATCCT
GGGCACCTGGCTGGACAGTACTCGGAGGATTTCTGTCACCCTCCGACTTTCCCTGCCTTA
AGCAGCTGGTGGCCTACGTGCAGCTTAACATGCCAGGCTTAGACCTGGAGACGCGGTGCC
ACCTTTCTCCTTGGCCAGCTGGAGCACTTGGAACCCATTGGGGGAGAGCCTGAGCCTTTG
TTACACTGGCCGGTTTAAAACAACTCCGAGAGTGGGCTAGTTTTAAACCACTTGGAGACC
CACCAAGGCCGGTCCACCCCGGACCGAACANTCCAACACANTCCGGTAAAGATTAAATTC
TCACACCCTCGGAGTCCAGGCTCAACCACTTGA
>OriGene 3' read for NM_006266 unedited
TTGTTTTCTTNNTTTTTTTTTAGTAGGAAACAGGGATTTTAATATAATATTCAAGTCTAG
CATTTGCTATTTACAACAAATAAATATTGGCCCCTTCCCCAATCAGTAAACAAACATTTT
TTTTTTCTTTTTGCTTTTTATACAAATATTCAATCACCCCACCCCCACCCCAAATCCTCC
TTCCTCACTAACCCCCGTCTTGCATGGTCTCGTAAAGCCCAGGACGCAGTGGTGAATGGC
ACTTGCAGTGGCATGAGATTCAACATCGATGGGACTCAGCTGGGACTGTCCTCACTCACC
GGGTGCAGAGTCTGGTCCATGAAGAGGGTTTCTCTCTCTGCTCCCAGGGGAGGGCTGGGG
TAAGCGGTGGGTGAGACTCCCTCACCTCAGTTGGCCCTGATGATGGAATCTTTGGTGCAT
CCTGAGAAAGGCTAGAGTGGTGGGAGGGGCCGGGACCCCTGCTAAGGTCACATGGCCACG
CCTGTGGGCATGAGAGAACTGCAATGGTTACAGTGGGTGCTCTGGGGGGCCCCTGGGCAC
AGGTGGCGTGGACCCACCCCTGGAAGGTGTCAGGGAAGTGTCAACCGCTGCGCCCGGGGC
CCGCTGGGCACCCTTCCCTCCCTCTTGGGTCTGCCCCTTGGCACAACATCCCCTCCTCCT
CCTGGTTCCCCGGCACTAATAAGCACCTAAAGCGCGCGTTGTGTTCCCCCGGAGGCCCGG
AACACCCTCGCCGTTTTGCCAGCCTTAATCCCCAACGCGGGCGGGGGCTCTAGGATAAAA
TTAGGTGTTTCGGTCCCCCGGGCCCGGGCGCCCGGAGCTGGGCGCCCC
Restriction Sites NotI-NotI     
ACCN NM_006266
Insert Size 4000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006266.2, NP_006257.1
RefSeq Size 3656 bp
RefSeq ORF 2745 bp
Locus ID 5900
Cytogenetics 9q34.13-q34.2
Domains RA, RasGEFN, RasGEF
Protein Pathways Colorectal cancer, Pancreatic cancer, Pathways in cancer
Gene Summary 'Guanine nucleotide dissociation stimulators (GDSs, or exchange factors), such as RALGDS, are effectors of Ras-related GTPases (see MIM 190020) that participate in signaling for a variety of cellular processes.[supplied by OMIM, Nov 2010]'
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

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