RALGDS (NM_006266) Human Untagged Clone

CAT#: SC100409

RALGDS (untagged)-Human ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RALGDS
• Synonyms: RalGEF; RGDS; RGF
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006266, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTGCAGCGCATGTGGGCCGAGGCGGCCGGGCCTGCTGGCGGCGCCGAGCCGCTGTTTCCGGGCTCCC
  GGCGGAGCCGCAGCGTGTGGGACGCCGTGCGCCTGGAGGTGGGCGTCCCCGACAGCTGCCCGGTGGTGCT
  GCACAGCTTCACGCAGCTAGACCCCGACCTGCCGCGCCCGGAGAGCTCCACGCAGGAGATCGGTGAGGAG
  CTGATCAACGGAGTCATCTACTCCATCTCCCTGCGCAAGGTGCAGCTGCACCACGGAGGCAACAAGGGGC
  AGCGCTGGCTCGGGTATGAGAATGAGTCGGCCCTGAACCTTTATGAGACTTGCAAGGTGCGGACCGTGAA
  GGCTGGCACGCTGGAGAAGCTGGTGGAGCACCTGGTGCCAGCCTTCCAGGGCAGCGACCTCTCCTACGTC
  ACCATCTTCCTGTGTACCTATAGAGCCTTCACCACCACCCAACAGGTCCTGGACCTGCTGTTCAAAAGGT
  ACGGTAGATGTGACGCCCTCACGGCCTCCTCTAGATACGGCTGCATCCTCCCCTATTCCGACGAGGATGG
  TGGACCCCAGGACCAACTTAAAAATGCCATCTCCTCCATCCTGGGCACCTGGCTGGACCAGTACTCGGAG
  GATTTCTGTCAACCTCCGGACTTTCCCTGCCTCAAGCAGCTGGTGGCCTACGTGCAGCTCAACATGCCAG
  GCTCAGACCTGGAGCGCCGTGCCCACCTTCTCCTGGCCCAGCTGGAGCACTCGGAACCCATTGAGGCAGA
  GCCTGAGGCTCTGTCACCAGTGCCAGCTCTAAAACCAACTCCAGAGCTCGAGCTAGCTCTAACACCAGCT
  CGAGCACCCAGCCCAGTGCCGGCTCCAGCCCCGGAGCCAGAGCCAGCTCCAACACCAGCTCCAGGTTCAG
  AGCTAGAAGTAGCTCCAGCACCAGCTCCGGAGCTCCAGCAGGCTCCAGAGCCAGCTGTGGGACTAGAATC
  GGCTCCAGCGCCAGCTCTGGAACTAGAGCCAGCTCCAGAACAGGATCCAGCTCCCTCACAAACTCTAGAG
  CTGGAGCCAGCTCCAGCACCAGTTCCATCATTACAGCCTTCCTGGCCTTCACCTGTGGTTGCAGAGAACG
  GGCTGAGTGAGGAGAAGCCTCACCTCTTGGTGTTCCCTCCAGATCTGGTGGCAGAGCAGTTTACACTGAT
  GGATGCGGAACTGTTCAAGAAGGTGGTGCCCTACCACTGCCTGGGCTCCATCTGGTCCCAGCGGGACAAG
  AAGGGCAAGGAGCACCTGGCGCCCACCATCCGCGCCACTGTCACCCAGTTCAACAGTGTGGCCAACTGTG
  TCATCACCACCTGCCTCGGGAACCGAAGCACGAAAGCCCCAGACAGGGCCAGGGTGGTGGAGCACTGGAT
  CGAGGTGGCCAGGGAGTGCCGGATCCTCAAGAACTTCTCGTCACTGTATGCCATCCTCTCTGCCCTGCAG
  AGCAACTCCATCCACCGTCTGAAGAAGACGTGGGAAGACGTTTCCAGGGACAGTTTCCGGATCTTTCAGA
  AGCTGTCAGAGATCTTCTCAGATGAGAACAACTACTCATTGAGCCGGGAGCTGCTCATCAAGGAGGGCAC
  CTCCAAGTTTGCCACCCTGGAGATGAACCCCAAGAGAGCCCAGAAACGGCCGAAGGAGACGGGCATCATC
  CAGGGCACCGTTCCCTACCTGGGCACGTTCCTCACCGACCTGGTGATGCTGGACACTGCCATGAAGGACT
  ATCTGTATGGCAGACTCATCAACTTTGAGAAGAGGAGGAAGGAGTTCGAGGTGATCGCCCAGATCAAGCT
  GCTGCAGTCGGCCTGCAACAACTACAGCATCGCGCCAGATGAGCAATTTGGGGCCTGGTTCCGGGCCGTG
  GAGCGGCTCAGCGAGACTGAGAGCTACAACCTGTCGTGCGAGCTGGAGCCCCCATCCGAGTCAGCCAGCA
  ACACCCTCAGGACCAAGAAGAACACAGCCATTGTCAAGCGCTGGAGCGACCGCCAGGCCCCCAGCACTGA
  GCTCAGTACCAGTGGCAGCTCCCACTCCAAGTCCTGTGACCAGCTCAGGTGTGGCCCCTACCTCAGCAGC
  GGGGACATCGCTGACGCGCTCAGCGTGCACTCGGCCGGCTCCTCTAGCTCCGACGTGGAGGAGATCAACA
  TCAGCTTCGTCCCGGAGTCTCCTGATGGCCAGGAAAAGAAGTTCTGGGAATCAGCCTCACAGTCATCCCC
  GGAGACCTCCGGCATCAGCTCAGCCTCCAGCAGCACCTCGTCCTCCTCAGCCTCCACCACGCCCGTGGCT
  GCCACACGCACCCACAAGCGCTCTGTCTCAGGGCTCTGCAACTCCAGCTCCGCGCTGCCGCTCTACAACC
  AGCAGGTGGGCGACTGCTGTATCATCCGCGTCAGCCTGGACGTGGACAATGGCAACATGTACAAGAGCAT
  CCTGGTGACCAGCCAAGATAAGGCTCCGGCTGTAATCCGCAAGGCCATGGACAAACACAACCTGGAGGAG
  GAGGAGCCGGAGGACTATGAGCTGCTGCAGATTCTCTCAGATGACCGGAAGCTGAAGATCCCTGAAAACG
  CCAACGTCTTCTATGCCATGAACTCTACCGCCAACTATGACTTTGTCCTCAAGAAGCGGACCTTCACCAA
  GGGAGTGAAGGTCAAGCACGGAGCCAGCTCCACCCTCCCTCGCATGAAGCAGAAAGGACTCAAGATTGCC
  AAGGGCATCTTCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006266 unedited
  AGGCGGCCGCGCAATTCGCACGAGGGCCGCGCGGGCCGCGCCGCCGCCCCCGCCGCGCGC
  CGGGCCGGCCCCGCGCCCGCGCTGCGAGCCTTCCATGGTGCAGCGCATGTGGGCCGAGGC
  GGCCGGGCCTGCTGGCGGCGCCGAGCCGCTGTTTCCGGGCTCCCGGCGGAGCCGCAGCGT
  GTGGGACGCCGTGCGCCTGGAGGTGGGCGTCCCCGACAGCTGCCCGGTGGTGCTGCACAG
  CTTCACGCAGCTAGACCCCGACCTGCCGCGCCCGGAGAGCTCCACGCAGGAGATCGGTGA
  GGAGCTGATCAACGGAGTCATCTACTCCATCTCCCTGCGCAAGGTGCAGCTGCACCACGG
  AGGCAACAAGGGGCAGCGCTGGCTCGGGTATGAGAATGAGTCGGCCCTGAACCTTTATGA
  GACTTGCAAGGTGCGGACCGTGAAGGCTGGCACGCTGGAGAAGCTGGTGGAGCACCTGGT
  GCCAGCCTTCCAGGGCAGCGACCTCTCCTACGTCACCATCTTCCTGTGTACCTATAGAGC
  CTTCACCACCACCCAACAGGTCCTGGACCTGCTGTTCAAAAGATACGGCTGCATCCTCCC
  CTATTCCGACGAGGATGGTGGACCCCAGGACCAACTTAANAATGCCATCTCCTNCATCCT
  GGGCACCTGGCTGGACAGTACTCGGAGGATTTCTGTCACCCTCCGACTTTCCCTGCCTTA
  AGCAGCTGGTGGCCTACGTGCAGCTTAACATGCCAGGCTTAGACCTGGAGACGCGGTGCC
  ACCTTTCTCCTTGGCCAGCTGGAGCACTTGGAACCCATTGGGGGAGAGCCTGAGCCTTTG
  TTACACTGGCCGGTTTAAAACAACTCCGAGAGTGGGCTAGTTTTAAACCACTTGGAGACC
  CACCAAGGCCGGTCCACCCCGGACCGAACANTCCAACACANTCCGGTAAAGATTAAATTC
  TCACACCCTCGGAGTCCAGGCTCAACCACTTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006266 unedited
  TTGTTTTCTTNNTTTTTTTTTAGTAGGAAACAGGGATTTTAATATAATATTCAAGTCTAG
  CATTTGCTATTTACAACAAATAAATATTGGCCCCTTCCCCAATCAGTAAACAAACATTTT
  TTTTTTCTTTTTGCTTTTTATACAAATATTCAATCACCCCACCCCCACCCCAAATCCTCC
  TTCCTCACTAACCCCCGTCTTGCATGGTCTCGTAAAGCCCAGGACGCAGTGGTGAATGGC
  ACTTGCAGTGGCATGAGATTCAACATCGATGGGACTCAGCTGGGACTGTCCTCACTCACC
  GGGTGCAGAGTCTGGTCCATGAAGAGGGTTTCTCTCTCTGCTCCCAGGGGAGGGCTGGGG
  TAAGCGGTGGGTGAGACTCCCTCACCTCAGTTGGCCCTGATGATGGAATCTTTGGTGCAT
  CCTGAGAAAGGCTAGAGTGGTGGGAGGGGCCGGGACCCCTGCTAAGGTCACATGGCCACG
  CCTGTGGGCATGAGAGAACTGCAATGGTTACAGTGGGTGCTCTGGGGGGCCCCTGGGCAC
  AGGTGGCGTGGACCCACCCCTGGAAGGTGTCAGGGAAGTGTCAACCGCTGCGCCCGGGGC
  CCGCTGGGCACCCTTCCCTCCCTCTTGGGTCTGCCCCTTGGCACAACATCCCCTCCTCCT
  CCTGGTTCCCCGGCACTAATAAGCACCTAAAGCGCGCGTTGTGTTCCCCCGGAGGCCCGG
  AACACCCTCGCCGTTTTGCCAGCCTTAATCCCCAACGCGGGCGGGGGCTCTAGGATAAAA
  TTAGGTGTTTCGGTCCCCCGGGCCCGGGCGCCCGGAGCTGGGCGCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006266
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006266.2, NP_006257.1
• RefSeq Size: 3656 bp
• RefSeq ORF: 2745 bp
• Locus ID: 5900
• Cytogenetics: 9q34.13-q34.2
• Domains: RA, RasGEFN, RasGEF
• Protein Pathways: Colorectal cancer, Pancreatic cancer, Pathways in cancer
• Gene Summary: 'Guanine nucleotide dissociation stimulators (GDSs, or exchange factors), such as RALGDS, are effectors of Ras-related GTPases (see MIM 190020) that participate in signaling for a variety of cellular processes.[supplied by OMIM, Nov 2010]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).