PIGC (NM_153747) Human Untagged Clone

CAT#: SC100523

PIGC (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class C (PIGC), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIGC
• Synonyms: GPI2; GPIBD16; MRT62
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100523 sequence for NM_153747 edited (data generated by NextGen Sequencing)
  ATGTATGCTCAACCTGTGACTAACACCAAGGAGGTCAAGTGGCAGAAGGTCTTGTATGAG
  CGACAGCCCTTTCCTGATAACTATGTGGACCGGCGATTCCTGGAAGAGCTCCGGAAAAAC
  ATCCATGCTCGGAAATACCAATATTGGGCTGTGGTATTTGAGTCCAGTGTGGTGATCCAG
  CAGCTGTGCAGTGTTTGTGTTTTTGTGGTTATCTGGTGGTATATGGATGAGGGTCTTCTG
  GCCCCCCATTGGCTTTTAGGGACTGGCCTGGCTTCTTCACTGATTGGGTATGTTTTGTTT
  GATCTCATTGATGGAGGTGAAGGGCGGAAGAAGAGTGGGCAGACCCGGTGGGCTGACCTG
  AAGAGTGCCCTAGTCTTCATTACTTTCACTTATGGGTTTTCACCAGTGCTGAAGACCCTT
  ACAGAGTCTGTCAGCACTGACACCATCTATGCCATGTCAGTCTTCATGCTGTTAGGCCAT
  CTCATCTTTTTTGACTATGGTGCCAATGCTGCCATTGTATCCAGCACACTATCCTTGAAC
  ATGGCCATCTTTGCTTCTGTATGCTTGGCATCACGTCTTCCCCGGTCCCTGCATGCCTTC
  ATCATGGTGACATTTGCCATTCAGATTTTTGCCCTGTGGCCCATGTTGCAGAAGAAACTA
  AAGGCATGTACTCCCCGGAGCTATGTGGGGGTCACACTGCTTTTTGCATTTTCAGCCGTG
  GGAGGCCTACTGTCCATTAGTGCTGTGGGAGCCGTACTCTTTGCCCTTCTGCTGATGTCT
  ATCTCATGTCTGTGTTCATTCTACCTCATTCGCTTGCAGCTTTTTAAAGAAAACATTCAT
  GGGCCTTGGGATGAAGCTGAAATCAAGGAAGACTTGTCCAGGTTCCTCAGTTAA
  
  Clone variation with respect to NM_153747.1
  267 t=>c;796 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_153747 unedited
  TAAATTTTGTATACGACTCACTATAGGCGGCCGCGAAATTCGCACGAGGCTGGGTGCCGC
  CGTCCCCCAGCTGCGCAACCCTAGGAACTCTCGGGAACTTGGCTGTGTTGTCCTGCCTCA
  GAGACAAATTCATCTATTGTAGGCCTAGCCCCTGCCTTTGAAAACAAGGAAAGGTTGGTA
  GAACATCAACACAGCATGGAATTTCCAGGGAGGTCTCATTTCAAAACTTCATAAAGAACA
  AGAACCACCTGGACTTCTGTGAGGGCGATGATTAAACTGGCCTGAGTTTGAATGAAAGGA
  TAATGTATGCTCAACCTGTGACTAACACCAAGGAGGTCAAGTGGCAGAAGGTCTTGTATG
  AGCGACAGCCCTTTCCTGATAACTATGTGGACCGGCGATTCCTGGAAGAGCTCCGGAAAA
  ACATCCATGCTCGGAAATACCAATATTGGGCTGTGGTATTTGAGTCCAGTGTGGTGATCC
  AGCAGCTGTGCAGTGTTTGTGTTTTTGTGGTTATCTGGTGGTTATGGATGAGGGTCTTCT
  GGCCCCCCATTGGCTTTTAGGGACTGGCCTGGCTTCTTCACTGATTGGGTATGTTTTGTT
  TGATCTCATTGATGGAGGTGAAGGGCGGAAGAAGAGTGGGCAGACCCGGTGGGCTGACCT
  GAAGAGTGCCCTAGTCTTCATTACTTTCACTTATGGGTTTTCACCAGTGCTGAAGACCCT
  TACAGAGTCTGTCAGCACTGACACCATCTATGCCATGTCAGTCTTCATGCTGTTAGGCCA
  TCTCATCTTTTTTGACTATGGTGCCAATGCTGCCATTGTATCCAGCACACTATCCTTTGA
  CATGGCNCATCTTGCTTCTGTATGCTTGGCATCACGTCTTCCCCGGTCCTGCATGCTTCA
  TCATGGTGACATTTGCATTCANATTTTTGCCTGTGGNNCCATGTGCAAAGAACTAAAGGC
  TGTACTCCCGNAGCTAGTTGGGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_153747 unedited
  NGGGCTACATTATGTACCGCGGCCGCGTTCTGANGATCGGTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGTTTTAAGGGGGGGTCAATTTTTCTT
  AAAAGAAAACCCCCTCACTTTCTATGTTACAGCATGGAATTCAAAAGGCCCCAAAAATTT
  TTTCCTCTCCCAAATAACCGAAAGGGTAAGCCCCCTCACCACCCAATCCTTTGGGTCATT
  TTTTTTGGGTTGGGGGCCTATTCTCTTACCACAATGACATGCTGGTTCCAAAAAGGAACC
  CTGGCTTTAAATTCTATACCAGTTAGGAGGCTAATCTATCAGCTTGGTTTAAAAATGGAA
  GGGATGGCCCAATTTAACTGAGGAACCCGGACAAGTCTTCCTTGATTTCAACTTCATCCC
  AAGGCCCATGAATGTTTTCTTTAAAAAACTGCCAGCCAATGAGGGAAAATGAACCCCGAC
  CTGAAATAAACCTCACCCAAAGGGCAAAAAGTACGGGTCCCCCAGCCCTTATGGACAGTA
  GGCCCCCCACGGGTGAAAATGCCAAAAGCAGGGGGACCCCCACATAACTCCGGGGAGTAC
  ATGCCCTTAATTTCTTCTGCAACATGGGCCCCAGGGCAAAAATCTGAATGGCAAATGTCC
  CCCTGATGAAAGCCTGCCGGGACCCGGGGAATACGTGATGCCCAGCCTACAGAACCCAAA
  ATGGCCCTGTTCAAAGATAAGGTGCCGGGATACAAGGGCCGGCTTGGCCCCCTAGTCCAA
  AAAAATGAAAAGGCCCTAACCCCCTGGAAAACCGGCCTGGGCTAAAATGGGGGACAAGGC
  TGAAAAAACTCTGTAAAGGTCCTTCACCCTCGGGGAAAACCCCTAGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_153747
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153747.1, NP_714969.1
• RefSeq Size: 1514 bp
• RefSeq ORF: 894 bp
• Locus ID: 5279
• Cytogenetics: 1q24.3
• Protein Families: Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
• Gene Summary: 'This gene encodes an endoplasmic reticulum associated protein that is involved in glycosylphosphatidylinositol (GPI) lipid anchor biosynthesis. The GPI lipid anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. The encoded protein is one subunit of the GPI N-acetylglucosaminyl (GlcNAc) transferase that transfers GlcNAc to phosphatidylinositol (PI) on the cytoplasmic side of the endoplasmic reticulum. Two alternatively spliced transcripts that encode the same protein have been found for this gene. A pseudogene on chromosome 11 has also been characterized. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) is the predominant transcript. Variants 1 and 2 encode the same protein.