SEPSECS (NM_153825) Human Untagged Clone

CAT#: SC100656

SEPSECS (untagged)-Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SEPSECS
• Synonyms: LP; MGC161491; Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase; SLA; SLA/LP; soluble liver antigen/liver pancreas antigen
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100656 sequence for NM_153825 edited (data generated by NextGen Sequencing)
  ATGAACCGCGAGAGCTTCGCGGCGGGAGAGCGGCTGGNNTCGCCGGCTTACGTGCGGCAG
  GGCTGTGAGGCCCGCCGCTCGCATGAGCACCTCATACGGCTGCTTCTGGAGAAGGGCAAG
  TGTCCAGAGAATGGCTGGGATGAAAGTACACTTGAACTCTTTTTACATGAACTTGCAATC
  ATGGACAGCAACAATTTCTTAGGCAATTGTGGTGTGGGAGAAAGGGAAGGGAGAGTGGCA
  TCCGCACTGGTTGCTCGTCGTCATTACAGGTTCATTCATGGCATTGGACGATCCGGTGAT
  ATTTCTGCTGTGCAACCAAAAGCTGCAGGCTCTAGCCTTTTGAACAAAATTACCAATTCT
  TTGGTCCTGGACATTATAAAGCTGGCTGGTGTCCATACAGTAGCCAACTGCTTTGTAGTT
  CCTATGGCAACTGGTATGAGTCTAACTCTGTGTTTCTTAACATTACGACACAAAAGACCA
  AAGGCAAAGTATATTATATGGCCACGAATAGACCAGAAGTCCTGCTTTAAATCCATGATC
  ACTGCAGGTTTTGAGCCTGTGGTGATAGAAAATGTTTTGGAAGGTGACGAGCTGCGTACA
  GACCTGAAAGCAGTGGAGGCTAAAGTCCAGGAACTTGGGCCTGATTGCATTCTGTGTATT
  CATTCTACTACATCCTGTTTTGCTCCAAGGGTGCCTGATAGATTAGAAGAACTGGCTGTG
  ATTTGTGCTAATTATGACATTCCACATATAGTTAATAATGCTTATGGAGTGCAGTCTTCA
  AAGTGTATGCATCTCATTCAGCAGGGGGCTCGAGTTGGTAGAATAGATGCTTTTGTTCAG
  AGCTTGGACAAAAATTTTATGGTTCCAGTAGGTGGTGCTATAATTGCTGGCTTTAATGAT
  TCATTCATTCAGGAAATCAGCAAGATGTATCCAGGAAGAGCTTCAGCTTCACCTTCTTTA
  GATGTCCTTATTACTTTATTGTCACTTGGATCAAATGGCTATAAGAAGCTACTAAAAGAA
  AGAAAGGAAATGTTTTCATATTTGTCCAACCAAATAAAGAAGTTGTCAGAAGCCTACAAT
  GAAAGACTGTTGCATACACCTCACAATCCCATATCTTTAGCTATGACACTTAAAACACTA
  GATGAACACCGTGACAAAGCTGTCACTCAGCTTGGCTCGATGCTTTTTACCAGACAGGTT
  TCTGGAGCCAGGGTTGTGCCTCTTGGGTCCATGCAAACTGTGAGTGGCTATACTTTCAGA
  GGCTTTATGTCACATACAAATAATTACCCTTGTGCTTACCTCAATGCTGCATCAGCCATC
  GGAATGAAGATGCAGGATGTGGACCTGTTCATAAAGAGACTTGACAGGTGTTTAAAGGCA
  GTAAGAAAAGAACGAAGTAAAGAGAGTGATGACAATTATGACAAAACTGAAGATGTGGAT
  ATTGAAGAAATGGCTTTAAAACTAGATAATGTACTTCTTGACACATACCAGGATGCTTCT
  TCATGA
  
  Clone variation with respect to NM_153825.2
  38 t=>n;39 g=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_153825 unedited
  GCAAATTTTGTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGGTCCAGGCGG
  CGAGTTGCATTCTGGTTCCGTCGTGTGTCTGGCGTGTGGAGCTAGTGCTCCCGCTTATTC
  GCCACTGTCGCCACCGCTGTCATGAACCGCGAGAGCTTCGCGGCGGGAGAGCGGCTGGTG
  TCGCCGGCTTACGTGCGGCAGGGCTGTGAGGCCCGCCGCTCGCATGAGCACCTCATACGG
  CTGCTTCTGGAGAAGGGCAAGTGTCCAGAGAATGGCTGGGATGAAAGTACACTTGAACTC
  TTTTTACATGAACTTGCAATCATGGACAGCAACAATTTCTTAGGCAATTGTGGTGTGGGA
  GAAAGGGAAGGGAGAGTGGCATCCGCACTGGTTGCTCGTCGTCATTACAGGTTCATTCAT
  GGCATTGGACGATCCGGTGATATTTCTGCTGTGCAACCAAAAGCTGCAGGCTCTAGCCTT
  TTGAACAAAATTACCAATTCTTTGGTCCTGGACATTATAAAGCTGGCTGGTGTCCATACA
  GTAGCCAACTGCTTTGTAGTTCCTATGGCAACTGGTATGAGTCTAACTCTGTGTTTCTTA
  ACATTACGACACAAAAGACCAAAGGCAAAGTATATTATATGGCCACGAATAGACCAGAAG
  TCCTGCTTTANATCCATGATCACTGCAGGTTTTGAGCCTGTGGTGATAGAAAATGTTNTG
  GGAGGTGACGAGCTGCGTACAGACCTGAAAGCAGTGGAGGCTTAAAGTCAGGAACTTGGG
  CCTGNATGCATTCTGTGTATTCATTCTACTACATNCTGGTTTGCTCCAGGGGTGCTGATA
  GATANAAGAACTGGCTGGGATTGTGCTAATATGACTTCCCATATAGTATATGCTTAGGGA
  GGCAGCTTCAAGGATGCTCTCATCANCGGGGCCTCAGTGTAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_153825 unedited
  CTTGGACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGACTCTAGAGTTG
  CAACTCTTCAGCTTTATTGGTTCAGCAATTTAGTCCCAAATTCTCATGAAAAATTTACAC
  TTTTTAATCCAGCATTTATTCCCTTGAAATGAGTTACTACACCTATGTAAATTTTTTTAA
  TGTTTGAACCCCAAAGATAAAATAAATACATTTTTAAATAACCTTCATGATATTCATAGT
  TAAATACTTCATCTCAAAATAGTCATTGACTTCCAAGGTGGTCAAGCCAGTGGCCATAGG
  CGTCTACCTTACACATGTCTGACCTCATGTTTACAAACAGTCTGGGACAATGTACAACGT
  CACCAAAATACCAGAAAATCCCTTTCTCATACATAGGTTACAATTCCAAATCAATAAGAA
  AAATGCAGCCCAGAAGGCAACACAGCATTCTCTACCATAGTGTTTTTAAAAGTATGTTCA
  ATGGGCTGTTAACAAATATTTCACTTTAAAAAAATGAGTTTCTCTTTGGATAAGGATATA
  TTAAAACTGGGTTATTTAGTGAAGGAATTCTTAGAGCCTTCAATATGCCAACAGGCAGGT
  TTGCAAAGGCAGCTCCCAAAATTATTTGTCTGTAAACATTTTCTCCCCATGAGCATCTTT
  TAGGAACATGATCCACAGAGCTGCGTTTGAGAAACATAGCTTTGGCATGGTGGCATGTTC
  AGAAGCTTCAGTAAATCTTCNAGCTATGACATCTCTATTTCTTAAATAGTCTTAAGTAAG
  TCTATTTCTTAATTAACAACAAAAATTATTGTAAGCCAGTAAGTCTGGAATTTAGACTCA
  GAAAATGAGCACTGGGCAAGTAGAGGGGATTACACCTGTTATATACTTGTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_153825
• Insert Size: 3840 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153825.1, NP_722547.1
• RefSeq Size: 5648 bp
• RefSeq ORF: 1326 bp
• Locus ID: 51091
• Cytogenetics: 4p15.2
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Gene Summary: The amino acid selenocysteine is the only amino acid that does not have its own tRNA synthetase. Instead, this amino acid is synthesized on its cognate tRNA in a three step process. The protein encoded by this gene catalyzes the third step in the process, the conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec). [provided by RefSeq, Mar 2011]
  Transcript Variant: This variant (1) represents the most predominant transcript, and encodes the longest isoform (1).