RASGRP 4 (RASGRP4) (NM_170603) Human Untagged Clone

CAT#: SC100713

RASGRP4 (untagged)-Human RAS guanyl releasing protein 4 (RASGRP4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RASGRP4
• Synonyms: guanyl nucleotide releasing protein 4; RAS guanyl releasing protein 4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100713 sequence for NM_170603 edited (data generated by NextGen Sequencing)
  ATGAACAGAAAAGACAGTAAGAGGAAGTCCCACCAGGAATGCACCGGAAAAACAGGAGGG
  CGAGGCCGGCCCCGCCAAGTGCGCCGCCACAAGACATGCCCCAGCCCTCGGGAAATCAGC
  AAGGTCATGGCTTCCATGAACCTGGGCCTGCTGAGTGAGGGCGGCTGCAGCGAAGATGAG
  CTGCTGGAGAAATGCATCCAGTCCTTCGATTCAGCTGGCAGCCTGTGCCACGAGGACCAC
  ATGCTCAACATGGTGCTGGCCATGCACAGCTGGGTGCTGCCGTCCGCCGACCTGGCTGCC
  CGCCTGCTGACCTCATACCAGAAGGCCACAGGGGACACCCAGGAGCTGAGACGGCTGCAG
  ATCTGTCACCTGGTCAGGTACTGGCTGATGCGACACCCTGAGGTGATGCACCAGGATCCC
  CAGCTAGAAGAAGTCATAGGTCGTTTCTGGGCCACCGTGGCCCGGGAGGGCAACTCAGCC
  CAGAGAAGACTGGGAGACTCTTCTGACCTCCTGAGCCCTGGTGGCCCTGGCCCCCCACTC
  CCAATGAGCAGCCCAGGCCTGGGCAAAAAGCGCAAAGTGTCCTTGCTTTTCGACCACTTG
  GAGACGGGGGAGCTGGCTCAGCACCTCACCTACCTGGAGTTCCGGTCCTTCCAGGCTATC
  ACGCCCCAGGACCTGCGGAGCTACGTTTTGCAGGGCTCAGTACGAGGCTGCCCGGCCCTG
  GAGGGCTCCGTAGGTCTCAGCAACAGCGTGTCCCGCTGGGTGCAGGTGATGGTGCTGAGC
  CGTCCCGGGCCCCTACAGCGTGCACAGGTGCTGGACAAGTTCATTCACGTGGCACAGAGG
  CTCCACCAGCTGCAGAATTTCAACACGCTGATGGCAGTCACAGGGGGCCTGTGTCACAGT
  GCCATCTCCAGACTCAAGGACTCCCATGCCCACCTGAGCCCTGACAGCACCAAGGCCCTC
  CTGGAGCTCACTGAGCTCCTTGCCTCCCACAACAACTACGCCCGCTACCGCCGCACCTGG
  GCTGGCTGCGCGGGTTTCCGGCTGCCTGTACTGGGCGTGCACCTCAAGGACCTGGTGTCC
  CTGCATGAGGCACAGCCCGACAGGTTGCCTGACGGCCGCCTGCACCTACCCAAGCTGAAC
  AACCTCTACCTGCGGCTGCAGGAGCTGGTGGCCCTCCAAGGGCAGCATCCACCCTGCAGC
  GCCAATGAGGATCTGCTGCACCTGCTCACGCTCTCCCTGGACCTCTTCTACACGGAAGAC
  GAGATCTATGAGCTTTCTTATGCCCGGGAGCCGCGTTGTCCCAAGAGCCTGCCACCCTCC
  CCCTTCAATGCACCTCTGGTGGTGGAGTGGGCCCCTGGTGTGACACCCAAGCCGGACAGG
  GTCACACTGGGTCGGCATGTGGAGCAGCTGGTGGAGTCTGTGTTCAAGAATTATGACCCT
  GAAGGCCGAGGAACAATCTCTCAGGAGGACTTTGAGCGACTCTCGGGCAATTTTCCCTTC
  GCCTGCCATGGGCTTCACCCACCCCCACGCCAGGGGAGAGGATCCTTCAGCAGAGAGGAG
  CTGACAGGGTACCTGCTCCGGGCCAGCGCCATCTGCTCCAAGTTGGGCCTGGCCTTCCTG
  CACACCTTCCATGAGGTCACCTTCCGAAAGCCTACCTTCTGCGACAGCTGCAGTGGCTTC
  CTCTGGGGTGTCACCAAGCAAGGCTACCGCTGTCGGGAGTGCGGGCTGTGTTGCCACAAA
  CACTGCAGAGACCAGGTGAAGGTAGAATGTAAGAAGAGGCCAGGGGCCAAGGGCGATGCA
  GGACCCCCCGGAGCTCCTGTCCCATCCACACCAGCTCCCCATGCCAGCTGTGGCTCCGAG
  GAAAATCACTCCTACACGCTATCCCTGGAGCCTGAGACTGGGTGCCAGCTTCGCCATGCC
  TGGACCCAGACTGAATCCCCACACCCTTCCTGGGAAACAGATACGGTCCCCTGCCCGGTG
  ATGGACCCACCATCAACTGCATCCTCCAAGCTGGATTCCTAG
  
  Clone variation with respect to NM_170603.1
  53 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_170603 unedited
  CTCTGGAATTTGTAATACGACTTACTATAGNNGGCGGCCGCGCAATTCGGCACCAGGCGG
  GAAGCATGAACAGAAAAGACAGTAAGAGGAAGTCCCACCAGGAATGCACCGGAAAAACAG
  GAGGGCGAGGCCGGCCCCGCCAAGTGCGCCGCCACAAGACATGCCCCAGCCCTCGGGAAA
  TCAGCAAGGTCATGGCTTCCATGAACCTGGGCCTGCTGAGTGAGGGCGGCTGCAGCGAAG
  ATGAGCTGCTGGAGAAATGCATCCAGTCCTTCGATTCAGCTGGCAGCCTGTGCCACGAGG
  ACCACATGCTCAACATGGTGCTGGCCATGCACAGCTGGGTGCTGCCGTCCGCCGACCTGG
  CTGCCCGCCTGCTGACCTCATACCAGAAGGCCACAGGGGACACCCAGGAGCTGAGACGGC
  TGCAGATCTGTCACCTGGTCAGGTACTGGCTGATGCGACACCCTGAGGTGATGCACCAGG
  ATCCCCAGCTAGAAGAAGTCATAGGTCGTTTCTGGGCCACCGTGGCCCGGGAGGGCAACT
  CAGCCCAGAGAAGACTGGGAGACTCTTCTGACCTCCTGAGCCCTGGTGGCCCTGGCCCCC
  CACTCCCAATGAGCAGCCCAGGCCTGGGCAAAAGCGCANAGTGTCCTTGCTTTTCGACCC
  TTGGAGACGGGGGAGCTGGCTCAGCACCTCACCTACCTGGAGTTCCGGTCCTTNNCAGCT
  ATCACGCCCCAGGACCTGCGAGCTACGTTTTGCAGGGCTCAGTACGAGGCTGCCCCGCCC
  TGGAGGGCTCCGTAAGTCTCAGCACAGCGTGTCCCNGCTGGTGCAGTGATGGTGCTGANC
  CCNTCCGGGCCCNTACACGTGCACAGTGCTGGACAGTTCATTCCTTGCACAGAGGCTCCA
  CAGCTGCCGATTCCACCCCTGATGGCATAAAGGGGGCCTGGTCCAATGGCATTTCAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_170603 unedited
  GAAAATAGTGAATTTNAATTTCGNACATCTGTGGGTACCTCTCTCTGTGGGGGCCAATCG
  GGGTGTTGGGGTTTGTGAAGGGGTTGGGGGGGTCTCTGCTCCCTGGGACTGAATGGGCCC
  CTGCTGCTCGACCCTCAAAGTTCTTTGCCCTATAGCACATCTATCCTGTGCCGTGGGCAT
  GGGTAAGGAGACATTCAGGCATCAGACAAGCATCTGTCTTGTTCCTACCACCTGCCAGCC
  CTGTTCTAGGCACTGGGGATGCAGCAGTGAATGACATTGATAGAAATCCCTGTTCTCCTG
  GAGTTGATGCTCCTGTTGAGGAAAGACAGACGTTCAACGAGATAAAGAGGGAAAAAGTAT
  AGGCTGTCAGAGGGAGCACATTCTATGCAGAAAAACAGGAGCTGGGCGTGGTGGCTCACG
  CCTGTATTCCCAGCACTTTGGGAGGCTGAGGCTGGTGGATCATGAGGTCAGGAGTTTGAG
  ACCATCTTGGCCAACATGGGGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGC
  ATGGTGGCGCGTGCCTGTAATCCCAGCTACTTGCGAGGCTGAGGCAGAAGAATCACTTTG
  ACCCAGNGAGCGGAAGTTGCAGTGAGCTGAGATTGCATCATTGCACTCCAGCCTGGGTGA
  CAGAGCAAGACTCTGTCTAAAAAAAAAAAGAAATGGAATGTAGGGAGTTGGTAAGAATAA
  GCCCTGAGTCTTAATGTTTTCAACACACAGGCCGACAGAATCCTAAATGCAGGGGAAAGG
  GAGCACGGGATTACCATCCCCCAGGATGCCAAATAAGAAAGTCACTTCCCGTGGAAAAAG
  GAGACCGGCCTCCCACCTTAAAGCCACTTTGGATTGGGAAAGCCGGTTGCCTTTGGGGGC
  CCTCCTTTTGGGAACCCTGCCAAAATTTACCGCAGGACCTAGACCTGACGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_170603
• ORF Size: 2634 bp
• Insert Size: 3000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_170603.1, NP_733748.1
• RefSeq Size: 2634
• RefSeq ORF: 2634
• Locus ID: 115727
• Domains: RasGEF, DAG_PE-bind
• Protein Families: Druggable Genome
• Protein Pathways: MAPK signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]
  Transcript Variant: This variant (1) encodes the longest isoform (1).