NFAT2 (NFATC1) (NM_172390) Human Untagged Clone

CAT#: SC101076

NFATC1 (untagged)-Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (NFATC1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NFATC1
• Synonyms: NF-ATC; NF-ATc1.2; NFAT2; NFATc
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC101076 sequence for NM_172390 edited (data generated by NextGen Sequencing)
  ATGCCAAGCACCAGCTTTCCAGTCCCTTCCAAGTTTCCACTTGGCCCTGCGGCTGCGGTC
  TTCGGGAGAGGAGAAACTTTGGGGCCCGCGCCGCGCGCCGGCGGCACCATGAAGTCAGCG
  GAGGAAGAACACTATGGCTATGCATCCTCCAACGTCAGCCCCGCCCTGCCGCTCCCCACG
  GCGCACTCCACCCTGCCGGCCCCGTGCCACAACCTTCAGACCTCCACACCGGGCATCATC
  CCGCCGGCGGATCACCCCTCGGGGTACGGAGCAGCTTTGGACGGTGGGCCCGCGGGCTAC
  TTCCTCTCCTCCGGCCACACCAGGCCTGATGGGGCCCCTGCCCTGGAGAGTCCTCGCATC
  GAGATAACCTCGTGCTTGGGCCTGTACCACAACAATAACCAGTTTTTCCACGATGTGGAG
  GTGGAAGACGTCCTCCCTAGCTCCAAACGGTCCCCCTCCACGGCCACGCTGAGTCTGCCC
  AGCCTGGAGGCCTACAGAGACCCCTCGTGCCTGAGCCCGGCCAGCAGCCTGTCCTCCCGG
  AGCTGCAACTCAGAGGCCTCCTCCTACGAGTCCAACTACTCGTACCCGTACGCGTCCCCC
  CAGACGTCGCCATGGCAGTCTCCCTGCGTGTCTCCCAAGACCACGGACCCCGAGGAGGGC
  TTTCCCCGCGGGCTGGGGGCCTGCACACTGCTGGGTTCCCCGCGGCACTCCCCCTCCACC
  TCGCCCCGCGCCAGCGTCACTGAGGAGAGCTGGCTGGGTGCCCGCTCCTCCAGACCCGCG
  TCCCCGTGCAACAAGAGGAAGTACAGCCTCAACGGCCGGCAGCCGCCCTACTCACCCCAC
  CACTCGCCCACGCCGTCCCCGCACGGCTCCCCGCGGGTCAGCGTGACCGACGACTCGTGG
  TTGGGCAACACCACCCAGTACACCAGCTCGGCCATCGTGGCCGCCATCAACGCGCTGACC
  ACCGACAGCAGCCTGGACCTGGGAGATGGCGTCCCTGTCAAGTCCCGCAAGACCACCCTG
  GAGCAGCCGCCCTCAGTGGCGCTCAAGGTGGAGCCCGTCGGGGAGGACCTGGGCAGCCCC
  CCGCCCCCGGCCGACTTCGCGCCCGAAGACTACTCCTCTTTCCAGCACATCAGGAAGGGC
  GGCTTCTGCGACCAGTACCTGGCGGTGCCGCAGCACCCCTACCAGTGGGCGAAGCCCAAG
  CCCCTGTCCCCTACGTCCTACATGAGCCCGACCCTGCCCGCCCTGGACTGGCAGCTGCCG
  TCCCACTCAGGCCCGTATGAGCTTCGGATTGAGGTGCAGCCCAAGTCCCACCACCGAGCC
  CACTACGAGACGGAGGGCAGCCGGGGGGCCGTGAAGGCGTCGGCCGGAGGACACCCCATC
  GTGCAGCTGCATGGCTACTTGGAGAATGAGCCGCTGATGCTGCAGCTTTTCATTGGGACG
  GCGGACGACCGCCTGCTGCGCCCGCACGCCTTCTACCAGGTGCACCGCATCACAGGGAAG
  ACCGTGTCCACCACCAGCCACGAGGCCATCCTCTCCAACACCAAAGTCCTGGAGATCCCA
  CTCCTGCCGGAGAACAGCATGCGAGCCGTCATTGACTGTGCCGGAATCCTGAAACTCAGA
  AACTCCGACATTGAACTTCGGAAAGGAGAGACGGACATCGGGAGGAAGAACACACGGGTA
  CGGCTGGTGTTCCGCGTTCACGTCCCGCAACCCAGCGGCCGCACGCTGTCCCTGCAGGTG
  GCCTCCAACCCCATCGAATGCTCCCAGCGCTCAGCTCAGGAGCTGCCTCTGGTGGAGAAG
  CAGAGCACGGACAGCTATCCGGTCGTGGGCGGGAAGAAGATGGTCCTGTCTGGCCACAAC
  TTCCTGCAGGACTCCAAGGTCATTTTCGTGGAGAAAGCCCCAGATGGCCACCATGTCTGG
  GAGATGGAAGCGAAAACTGACCGGGACCTGTGCAAGCCGAATTCTCTGGTGGTTGAGATC
  CCGCCATTTCGGAATCAGAGGATAACCAGCCCCGTTCACGTCAGTTTCTACGTCTGCAAC
  GGGAAGAGAAAGCGAAGCCAGTACCAGCGTTTCACCTACCTTCCCGCCAACGGTAACGCC
  ATCTTTCTAACCGTAAGCCGTGAACATGAGCGCGTGGGGTGCTTTTTCTAA
  
  Clone variation with respect to NM_172390.1
  786 t=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_172390 unedited
  NGAAGGAAAAAGGCCCAACACCACCCCTTATACANNNNGTTTTTACACCCGCCCGTACCC
  GCATAGGGCGGTAGGCGTGTCCGGTGGNGAGTCTATATAAGCAGAGCTCATTTAGGTGAC
  ACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGAACT
  CGCCGGCGGAGTCGCCGCGCCAGATCCCAGCAGCAGGGCGCGGGCACCGGGGCGCGGGCA
  GGGCTCGGAGCCACCGCGCAGGTCCTAGGGCCGCGGCCGGGCCCCGCCACGCGCGCACAC
  GCCCCTCGATGACTTTCCTCCGGGGCGCGCGGCGCTGAGCCCGGGGCGAGGGCTGTCTTC
  CCGGAGACCCGACCCCGGCAGCGCGGGGCGCCCGCTTCTCCTGTGCCTCCGCCCGCCGCT
  CCACTCCCCGCCGCCGCCGCGCGGATGCCAAGCACCAGCTTTCCAGTCCCTTCCAAGTTT
  CCACTTGGCCCTGCGGCTGCGGTCTTCGGGAGAGGAGAAACTTTGGGGCCCGCGCCGCGC
  GCCGGCGGCACCATGAAGTCAGCGGAGGAAGAACACTATGGCTATGCATCCTCCAACGTC
  AGCCCCGCCCTGCCGCTCCCCACGGCGCACTCCACCCTGCCGGCCCCGTGCCACAACCTT
  CAGACCTCCACACCGGGCATCATCCCGCCGGCGGATCACCCCTCGGNGTACGGAGCAGCT
  NTGGACGGTGGGCCCGCGGGCTACTTCCTCTCCTCCGGCCACACCAGGCCTGATGGNGCC
  CCTGCCCTGGAGAGTCCTCGCATCGAGATAACCTCGTGCTTGGGCCTGTACCACAACAAT
  AACCAGTTNTTCCACGATGTGGNAGGTGGGAGACGTCCTCCCTAGCTCCAAACGGTCCCC
  CTCCACGGCCACGCTGAATCTGCCCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_172390 unedited
  GAAAATCAGGTACCGTGCCGCAATCGAGGATTNGTTTTTTTTCTTTTTTTTTAAAAGCAA
  AGTTTATTCCTAAAGTGCCTGCAAAATAGCTATACCTATTTGCCTTTTCACCGTAAGCAG
  CTTTAGGGTGCAAATGCACACCAGGACCCCCTCAGTTTTTTGGAGCAGAAGAGCCATGTT
  TTCCCCTTGGCTGTTATACATTCGCAAGAAGCAAAATATCCGTGACTATCTGAGTATGCA
  ACCCCCGCCCCCCACCCCCAACAGGGATCAAGGAAAATGCATCCGAAGCTTCCCTTTTCC
  TTTCCTTTTCAGGGCATCGATGGCTCGGTCTCACTAACGGGACATCACACAGTTAAATGT
  GCAACACGCCACGCTGCTTTACGGCGACGTCGTTTCTGCGTCTTTAGAAAAAGCACCCCA
  CGCGCTCATGTTCACGGCTTACGGTTAGAAAGATGGCGTTACCGTTGGCGGGAAGGTAGG
  TGAAACGCTGGTACTGGCTTCGCTTTTTCTTCCCGTTGCAGACGTAGAAACTGACGTGAA
  CGGGGCTGGGTATCCTCTGATTCCGAAATGGCGGGATCTCAACCACCAGAGAATTCCGCT
  TGCACAAGGCCCGGGAAGTTTTCGCTTTCATCTCCCAGACATGGTGGCCATCTGGGGGCT
  TTCTCACGAAAAATACCCTTGGAGTCCTCGCAGAAAGTTTTGGGCAGAAAAGACCATTTT
  CTTTCCGCCCACGACCGGATAGCTGGCCGTGCTTTTGTTTTTCCACCAAAGCAGCTTCCT
  GGACTTGAGCCTGGGAGCATTCGTTGGGGGTTGGAGGCCCCTCTGTGGGACAACTTTCCG
  GCCCTTGTGTTGCGGGACCTTTACACCGGAAAACCAGCCGTCCCCGGGGGTTTTTTTTTC
  CGATTGGGGTTTTTCTTTTCCAAGATAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_172390
• Insert Size: 2300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172390.1, NP_765978.1
• RefSeq Size: 3140 bp
• RefSeq ORF: 2151 bp
• Locus ID: 4772
• Cytogenetics: 18q23
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Axon guidance, B cell receptor signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• Gene Summary: 'The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013]'
  Transcript Variant: This variant (1) lacks two exons and its transcription extends past a splice site that is used in variant 6, which results in a novel 3' coding region and 3' UTR, compared to variant 6. The encoded isoform (A) is shorter and has a distinct C-terminus, compared to isoform F.