MYO1F (NM_012335) Human Untagged Clone

CAT#: SC101088

MYO1F (untagged)-Human myosin IF (MYO1F)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYO1F
• Synonyms: MYO1F variant protein; myosin; myosin-ID; myosin IF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012335 edited
  GTGAACGGGCAGAAGCAGGGCCATGCCCAAGCCACCCCCAAGATCCCCCTGAACCTGCAC
  CTCCATCACGACCCATTCAGGAGCCTCCAGGAGCCCAGACACCAGCCCCCCACCATGGGC
  AGCAAGGAGCGCTTCCACTGGCAGAGCCACAACGTGAAGCAGAGCGGCGTGGATGACATG
  GTGCTTCTTCCCCAGATCACCGAAGACGCCATTGCCGCCAACCTCCGGAAGCGCTTCATG
  GACGACTACATCTTCACCTACATCGGCTCTGTGCTCATCTCTGTAAACCCCTTCAAGCAG
  ATGCCCTACTTCACCGACCGTGAGATCGACCTCTATCAGGGCGCGGCCCAGTATGAGAAT
  CCCCCGCACATCTACGCCCTCACGGACAACATGTACCGGAACATGCTTATCGACTGTGAG
  AACCAGTGTGTCATCATTAGTGGAGAGAGTGGAGCTGGGAAGACAGTGGCAGCCAAATAT
  ATCATGGGCTACATCTCCAAGGTGTCTGGCGGAGGCGAGAAGGTCCAGCACGTCAAAGAT
  ATCATCCTGCAGTCCAACCCGCTGCTCGAGGCCTTCGGCAACGCCAAGACTGTGCGCAAC
  AACAATTCCAGCCGCTTTGGCAAGTACTTTGAGATCCAGTTCAGCCGAGGTGGGGAGCCA
  GATGGGGGCAAGATCTCCAACTTCTTGCTGGAGAAGTCCCGCGTGGTCATGCAAAATGAA
  AATGAGAGGAACTTCCACATCTACTACCAGCTGCTGGAAGGGGCCTCCCAGGAGCAAAGG
  CAGAACCTGGGCCTCATGACACCGGACTACTATTACTACCTCAACCAATCGGACACCTAC
  CAGGTGGACGGCACGGACGACAGAAGCGACTTTGGTGAGACTCTGAGTGCTATGCAGGTT
  ATTGGGATCCCGCCCAGCATCCAGCAGCTGGTCCTGCAGCTCGTGGCGGGGATCTTGCAC
  CTGGGGAACATCAGTTTCTGTGAAGACGGGAATTACGCCCGAGTGGAGAGTGTGGACCTC
  CTGGCCTTTCCCGCCTACCTGCTGGGCATTGACAGCGGGCGACTGCAGGAGAAGCTGACC
  AGCCGCAAGATGGACAGCCGCTGGGGCGGGCGCAGCGAGTCCATCAATGTGACCCTCAAC
  GTGGAGCAGGCAGCCTACACCCGTGATGCCCTGGCCAAGGGGCTCTATGCCCGCCTCTTC
  GACTTCCTCGTGGAGGCCATCAACCGTGCTATGCAGAAACCCCAGGAAGAGTACAGCATC
  GGTGTGCTGGACATTTACGGCTTCGAGATCTTCCAGAAAAATGGCTTCGAGCAGTTTTGC
  ATCAACTTCGTCAATGAGAAGCTGCAGCAAATCTTTATCGAACTTACCCTGAAGGCCGAG
  CAGGAGGAGTATGTGCAGGAAGGCATCCGCTGGACTCCAATCCAGTACTTCAACAACAAG
  GTCGTCTGTGACCTCATCGAAAACAAGCTGAGCCCCCCAGGCATCATGAGCGTCTTGGAC
  GACGTGTGCGCCACCATGCACGCCACGGGCGGGGGAGCAGACCAGACACTGCTGCAGAAG
  CTGCAGGCGGCTGTGGGGACCCACGAGCATTTCAACAGCTGGAGCGCCGGCTTCGTCATC
  CACCACTACGCTGGCAAGGTCTCCTACGACGTCAGCGGCTTCTGCGAGAGGAACCGAGAC
  GTTCTCTTCTCCGACCTCATAGAGCTGATGCAGACCAGTGAGCAGGCCTTCCTCCGGATG
  CTCTTCCCCGAGAAGCTGGATGGAGACAAGAAGGGGCGCCCCAGCACCGCCGGCTCCAAG
  ATCAAGAAACAAGCCAACGACCTGGTGGCCACACTGATGAGGTGCACACCCCACTACATC
  CGCTGCATCAAACCCAACGAGACCAAGAGGCCCCGAGACTGGGAGGAGAACAGAGTCAAG
  CACCAGGTGGAATACCTGGGCCTGAAGGAGAACATCAGGGTGCGCAGAGCCGGCTTCGCC
  TACCGCCGCCAGTTCGCCAAATTCCTGCAGAGGTATGCCATTCTGACCCCCGAGACGTGG
  CCGCGGTGGCGTGGGGACGAACGCCAGGGCGTCCAGCACCTGCTTCGGGCGGTCAACATG
  GAGCCCGACCAGTACCAGATGGGGAGCACCAAGGTCTTTGTCAAGAACCCAGAGTCGCTT
  TTCCTCCTGGAGGAGGTGCGAGAGCGAAAGTTCGATGGCTTTGCCCGAACCATCCAGAAG
  GCCTGGCGGCGCCACGTGGCTGTCCGGAAGTACGAGGAGATGCGGGAGGAAGCTTCCAAC
  ATCCTGCTGAACAAGAAGGAGCGGAGGCGCAACAGCATCAATCGGAACTTCGTCGGGGAC
  TACCTGGGGCTGGAGGAGCGGCCCGAGCTGCGTCAGTTCCTGGGCAAGAGGGAGCGGGTG
  GACTTCGCCGATTCGGTCACCAAGTACGACCGCCGCTTCAAGCCCATCAAGCGGGACTTG
  ATCCTGACGCCCAAGTGTGTGTATGTGATTGGGCGAGAGAAAGTGAAGAAGGGACCTGAG
  AAGGGCCAGGTGTGTGAAGTCTTGAAGAAGAAAGTGGACATCCAGGCTCTGCGGGGAGTC
  TCCCTCAGCACGCGACAGGACGACTTCTTCATCCTCCAAGAGGATGCCGCCGACAGCTTC
  CTGGAGAGCGTCTTCAAGACCGAGTTTGTCAGCCTTCTGTGCAAGCGCTTCGAGGAGGCG
  ACGCGGAGGCCCCTGCCCCTCACCTTCAGCGACACACTACAGTTTCGGGTGAAGAAGGAG
  GGCTGGGGCGGTGGCGGCACCCGCAGCGTCACCTTCTCCCGCGGCTTCGGCGACTTGGCA
  GTGCTCAAGGTTGGCGGTCGGACCCTCACGGTCAGCGTGGGCGATGGGCTGCCCAAGAGC
  TCCAAGCCTACGCGGAAGGGAATGGCCAAGGGAAAACCTCGGAGGTCGTCCCAAGCCCCT
  ACCCGGGCGGCCCCTGCGCCCCCCAGAGGCATGGATCGCAATGGGGTGCCCCCCTCTGCC
  AGAGGGGGCCCCCTGCCCCTGGAGATCATGTCTGGAGGGGGCACCCACAGGCCTCCCCGG
  GGCCCTCCGTCCACATCCCTGGGAGCCAGCAGACGACCCCGGGCACGTCCGCCCTCAGAG
  CACAACACAGAATTCCTCAACGTGCCTGACCAGGGCATGGCCGGCATGCAGAGGAAGCGC
  AGCGTGGGGCAACGGCCAGTGCCTGGTGTGGGCCGACCCAAGCCCCAGCCTCGGACACAT
  GGTCCCAGGTGCCGGGCCCTATACCAGTACGTGGGCCAAGATGTGGACGAGCTGAGCTTC
  AACGTGAACGAGGTCATTGAGATCCTCATGGAAGATCCCTCGGGCTGGTGGAAGGGCCGG
  CTTCACGGCCAGGAGGGCCTTTTCCCAGGAAACTACGTGGAGAAGATCTGAGCTGGGCCC
  TGGGATACTGCCTTCTCTTTCGCCCGCCTATCTGCCTGCCGGCCTGGTGGGGAGCCAGGC
  CCTGCCAATGAGAGCCTCGTTTACCTGGGCTGCAATAGCCTAAAAGTCCAGTCCTTTGGC
  CTCCAGTCCTGCCCAGGCCCTGGGTCACCAGGTCACTGCTGCAGCCCCCGCCCCTGGGCC
  CTGGTCTTCCTCCAACATCACACCTGCTGCCCATTCTCCATTTCTGTGTGTGTCAAAGGG
  GACTAACAGCAGAATCTACCTCCCAACTGCCATGTGATTAAGAAATGGGTCTTGAGTCCT
  GTGCTGTTGGCAAAGTGCCAGGCACAGTTGGGGAGGGGGGGGTCCTTAACAAGCGTGACT
  TTGCTCATTCTGTCATCACTAAGGCAATAAACCTTTGCCAGGTGAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012335
• Insert Size: 3800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfectmatch to NM_012335.2.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012335.2, NP_036467.2
• RefSeq Size: 3860 bp
• RefSeq ORF: 3297 bp
• Locus ID: 4542
• Cytogenetics: 19p13.2
• Domains: IQ, SH3, myosin_head
• Gene Summary: 'Myosins are molecular motors that use the energy from ATP hydrolysis to generate force on actin filaments. The protein encoded by this gene is an unconventional myosin that may be involved in the intracellular movement of membrane-enclosed compartments. There is evidence to suggest that mutations in this gene can result in hearing loss. [provided by RefSeq, Jan 2017]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).