MYO1F (NM_012335) Human Untagged Clone

CAT#: SC101088

MYO1F (untagged)-Human myosin IF (MYO1F)


  "NM_012335" in other vectors (6)

Reconstitution Protocol

USD 1,100.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol MYO1F
Synonyms MYO1F variant protein; myosin; myosin-ID; myosin IF
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_012335 edited
GTGAACGGGCAGAAGCAGGGCCATGCCCAAGCCACCCCCAAGATCCCCCTGAACCTGCAC
CTCCATCACGACCCATTCAGGAGCCTCCAGGAGCCCAGACACCAGCCCCCCACCATGGGC
AGCAAGGAGCGCTTCCACTGGCAGAGCCACAACGTGAAGCAGAGCGGCGTGGATGACATG
GTGCTTCTTCCCCAGATCACCGAAGACGCCATTGCCGCCAACCTCCGGAAGCGCTTCATG
GACGACTACATCTTCACCTACATCGGCTCTGTGCTCATCTCTGTAAACCCCTTCAAGCAG
ATGCCCTACTTCACCGACCGTGAGATCGACCTCTATCAGGGCGCGGCCCAGTATGAGAAT
CCCCCGCACATCTACGCCCTCACGGACAACATGTACCGGAACATGCTTATCGACTGTGAG
AACCAGTGTGTCATCATTAGTGGAGAGAGTGGAGCTGGGAAGACAGTGGCAGCCAAATAT
ATCATGGGCTACATCTCCAAGGTGTCTGGCGGAGGCGAGAAGGTCCAGCACGTCAAAGAT
ATCATCCTGCAGTCCAACCCGCTGCTCGAGGCCTTCGGCAACGCCAAGACTGTGCGCAAC
AACAATTCCAGCCGCTTTGGCAAGTACTTTGAGATCCAGTTCAGCCGAGGTGGGGAGCCA
GATGGGGGCAAGATCTCCAACTTCTTGCTGGAGAAGTCCCGCGTGGTCATGCAAAATGAA
AATGAGAGGAACTTCCACATCTACTACCAGCTGCTGGAAGGGGCCTCCCAGGAGCAAAGG
CAGAACCTGGGCCTCATGACACCGGACTACTATTACTACCTCAACCAATCGGACACCTAC
CAGGTGGACGGCACGGACGACAGAAGCGACTTTGGTGAGACTCTGAGTGCTATGCAGGTT
ATTGGGATCCCGCCCAGCATCCAGCAGCTGGTCCTGCAGCTCGTGGCGGGGATCTTGCAC
CTGGGGAACATCAGTTTCTGTGAAGACGGGAATTACGCCCGAGTGGAGAGTGTGGACCTC
CTGGCCTTTCCCGCCTACCTGCTGGGCATTGACAGCGGGCGACTGCAGGAGAAGCTGACC
AGCCGCAAGATGGACAGCCGCTGGGGCGGGCGCAGCGAGTCCATCAATGTGACCCTCAAC
GTGGAGCAGGCAGCCTACACCCGTGATGCCCTGGCCAAGGGGCTCTATGCCCGCCTCTTC
GACTTCCTCGTGGAGGCCATCAACCGTGCTATGCAGAAACCCCAGGAAGAGTACAGCATC
GGTGTGCTGGACATTTACGGCTTCGAGATCTTCCAGAAAAATGGCTTCGAGCAGTTTTGC
ATCAACTTCGTCAATGAGAAGCTGCAGCAAATCTTTATCGAACTTACCCTGAAGGCCGAG
CAGGAGGAGTATGTGCAGGAAGGCATCCGCTGGACTCCAATCCAGTACTTCAACAACAAG
GTCGTCTGTGACCTCATCGAAAACAAGCTGAGCCCCCCAGGCATCATGAGCGTCTTGGAC
GACGTGTGCGCCACCATGCACGCCACGGGCGGGGGAGCAGACCAGACACTGCTGCAGAAG
CTGCAGGCGGCTGTGGGGACCCACGAGCATTTCAACAGCTGGAGCGCCGGCTTCGTCATC
CACCACTACGCTGGCAAGGTCTCCTACGACGTCAGCGGCTTCTGCGAGAGGAACCGAGAC
GTTCTCTTCTCCGACCTCATAGAGCTGATGCAGACCAGTGAGCAGGCCTTCCTCCGGATG
CTCTTCCCCGAGAAGCTGGATGGAGACAAGAAGGGGCGCCCCAGCACCGCCGGCTCCAAG
ATCAAGAAACAAGCCAACGACCTGGTGGCCACACTGATGAGGTGCACACCCCACTACATC
CGCTGCATCAAACCCAACGAGACCAAGAGGCCCCGAGACTGGGAGGAGAACAGAGTCAAG
CACCAGGTGGAATACCTGGGCCTGAAGGAGAACATCAGGGTGCGCAGAGCCGGCTTCGCC
TACCGCCGCCAGTTCGCCAAATTCCTGCAGAGGTATGCCATTCTGACCCCCGAGACGTGG
CCGCGGTGGCGTGGGGACGAACGCCAGGGCGTCCAGCACCTGCTTCGGGCGGTCAACATG
GAGCCCGACCAGTACCAGATGGGGAGCACCAAGGTCTTTGTCAAGAACCCAGAGTCGCTT
TTCCTCCTGGAGGAGGTGCGAGAGCGAAAGTTCGATGGCTTTGCCCGAACCATCCAGAAG
GCCTGGCGGCGCCACGTGGCTGTCCGGAAGTACGAGGAGATGCGGGAGGAAGCTTCCAAC
ATCCTGCTGAACAAGAAGGAGCGGAGGCGCAACAGCATCAATCGGAACTTCGTCGGGGAC
TACCTGGGGCTGGAGGAGCGGCCCGAGCTGCGTCAGTTCCTGGGCAAGAGGGAGCGGGTG
GACTTCGCCGATTCGGTCACCAAGTACGACCGCCGCTTCAAGCCCATCAAGCGGGACTTG
ATCCTGACGCCCAAGTGTGTGTATGTGATTGGGCGAGAGAAAGTGAAGAAGGGACCTGAG
AAGGGCCAGGTGTGTGAAGTCTTGAAGAAGAAAGTGGACATCCAGGCTCTGCGGGGAGTC
TCCCTCAGCACGCGACAGGACGACTTCTTCATCCTCCAAGAGGATGCCGCCGACAGCTTC
CTGGAGAGCGTCTTCAAGACCGAGTTTGTCAGCCTTCTGTGCAAGCGCTTCGAGGAGGCG
ACGCGGAGGCCCCTGCCCCTCACCTTCAGCGACACACTACAGTTTCGGGTGAAGAAGGAG
GGCTGGGGCGGTGGCGGCACCCGCAGCGTCACCTTCTCCCGCGGCTTCGGCGACTTGGCA
GTGCTCAAGGTTGGCGGTCGGACCCTCACGGTCAGCGTGGGCGATGGGCTGCCCAAGAGC
TCCAAGCCTACGCGGAAGGGAATGGCCAAGGGAAAACCTCGGAGGTCGTCCCAAGCCCCT
ACCCGGGCGGCCCCTGCGCCCCCCAGAGGCATGGATCGCAATGGGGTGCCCCCCTCTGCC
AGAGGGGGCCCCCTGCCCCTGGAGATCATGTCTGGAGGGGGCACCCACAGGCCTCCCCGG
GGCCCTCCGTCCACATCCCTGGGAGCCAGCAGACGACCCCGGGCACGTCCGCCCTCAGAG
CACAACACAGAATTCCTCAACGTGCCTGACCAGGGCATGGCCGGCATGCAGAGGAAGCGC
AGCGTGGGGCAACGGCCAGTGCCTGGTGTGGGCCGACCCAAGCCCCAGCCTCGGACACAT
GGTCCCAGGTGCCGGGCCCTATACCAGTACGTGGGCCAAGATGTGGACGAGCTGAGCTTC
AACGTGAACGAGGTCATTGAGATCCTCATGGAAGATCCCTCGGGCTGGTGGAAGGGCCGG
CTTCACGGCCAGGAGGGCCTTTTCCCAGGAAACTACGTGGAGAAGATCTGAGCTGGGCCC
TGGGATACTGCCTTCTCTTTCGCCCGCCTATCTGCCTGCCGGCCTGGTGGGGAGCCAGGC
CCTGCCAATGAGAGCCTCGTTTACCTGGGCTGCAATAGCCTAAAAGTCCAGTCCTTTGGC
CTCCAGTCCTGCCCAGGCCCTGGGTCACCAGGTCACTGCTGCAGCCCCCGCCCCTGGGCC
CTGGTCTTCCTCCAACATCACACCTGCTGCCCATTCTCCATTTCTGTGTGTGTCAAAGGG
GACTAACAGCAGAATCTACCTCCCAACTGCCATGTGATTAAGAAATGGGTCTTGAGTCCT
GTGCTGTTGGCAAAGTGCCAGGCACAGTTGGGGAGGGGGGGGTCCTTAACAAGCGTGACT
TTGCTCATTCTGTCATCACTAAGGCAATAAACCTTTGCCAGGTGAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAA
Restriction Sites NotI-NotI     
ACCN NM_012335
Insert Size 3800 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation The ORF of this clone has been fully sequenced and found to be a perfectmatch to NM_012335.2.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_012335.2, NP_036467.2
RefSeq Size 3860 bp
RefSeq ORF 3297 bp
Locus ID 4542
Cytogenetics 19p13.2
Domains IQ, SH3, myosin_head
Gene Summary 'Myosins are molecular motors that use the energy from ATP hydrolysis to generate force on actin filaments. The protein encoded by this gene is an unconventional myosin that may be involved in the intracellular movement of membrane-enclosed compartments. There is evidence to suggest that mutations in this gene can result in hearing loss. [provided by RefSeq, Jan 2017]'
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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