PDE8A (NM_002605) Human Untagged Clone

CAT#: SC101098

PDE8A (untagged)-Human phosphodiesterase 8A (PDE8A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE8A
• Synonyms: HsT19550
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002605 edited
  ATGGGCTGTGCCCCGAGCATCCACATTTCCGAGCGCCTGGTGGCCGAGGACGCGCCTAGC
  CCCGCGGCACCGCCGCTGTCGTCCGGCGGGCCGCGCCTCCCGCAGGGCCAGAAGACGGCC
  GCCTTGCCCCGGACCCGCGGCGCCGGCCTCTTGGAGTCGGAGCTTCGCGACGGCAGCGGC
  AAGAAGGTAGCAGTAGCTGATGTGCAGTTTGGCCCCATGAGATTTCATCAAGATCAACTT
  CAGGTACTTTTAGTGTTTACCAAAGAAGATAACCAATGTAATGGATTCTGCAGGGCATGT
  GAAAAAGCAGGGTTTAAGTGTACAGTTACCAAGGAGGCTCAGGCTGTCCTTGCCTGTTTC
  CTGGACAAACATCATGACATTATCATCATAGACCACAGAAATCCTCGACAGCTGGATGCA
  GAGGCACTGTGCAGGTCTATCAGATCATCAAAACTCTCAGAAAACACAGTTATTGTTGGT
  GTAGTACGCAGGGTGGATAGAGAAGAGTTGTCCGTAATGCCTTTCATTTCTGCTGGATTT
  ACAAGGAGGTATGTAGAAAACCCCAACATCATGGCCTGCTACAATGAACTGCTCCAGCTG
  GAGTTTGGAGAGGTGCGATCACAACTGAAACTCAGGGCTTGTAACTCAGTATTCACTGCA
  TTAGAAAACAGTGAAGATGCAATTGAAATTACAAGCGAAGACCGTTTTATACAGTATGCA
  AATCCTGCATTTGAAACAACAATGGGCTATCAGTCAGGTGAATTAATAGGGAAGGAGTTA
  GGAGAAGTGCCTATAAATGAAAAAAAGGCTGACTTGCTCGATACTATAAATTCATGCATC
  AGGATAGGCAAGGAGTGGCAAGGAATTTACTATGCCAAAAAGAAAAACGGAGATAATATA
  CAACAAAATGTGAAGATAATACCTGTCATTGGACAGGGAGGAAAAATTAGACACTATGTG
  TCCATTATCAGAGTGTGCAATGGCAACAATAAGGCTGAGAAAATATCCGAATGTGTTCAG
  TCTGACACTCATACAGATAATCAGACAGGCAAACATAAAGACAGGAGAAAAGGCTCACTA
  GACGTCAAAGCTGTTGCCTCCCGTGCAACTGAAGTTTCCAGCCAGAGACGACACTCTTCC
  ATGGCCCGGATACATTCCATGACAATTGAGGCGCCCATCACCAAGGTAATCAATATTATC
  AATGCTGCCCAGGAAAGTAGTCCCATGCCTGTGACAGAAGCCCTAGACCGTGTGCTGGAA
  ATTCTAAGAACCACTGAGTTATATTCACCACAGTTTGGTGCTAAAGATGATGATCCCCAT
  GCCAATGACCTTGTTGGGGGCTTAATGTCTGATGGTTTGCGAAGACTATCAGGGAATGAA
  TATGTTCTTTCAACAAAAAACACTCAAATGGTTTCAAGCAATATAATCACTCCCATCTCC
  CTTGATGATGTCCCACCACGGATAGCTCGGGCCATGGAAAATGAGGAATACTGGGACTTT
  GATATTTTTGAACTGGAGGCTGCCACCCACAATAGGCCTTTGATTTATCTTGGTCTCAAA
  ATGTTTGCTCGCTTTGGAATCTGTGAATTCTTACACTGCTCCGAGTCAACGCTAAGATCA
  TGGTTACAAATTATCGAAGCCAATTATCATTCCTCCAATCCCTACCACAATTCTACACAT
  TCTGCTGATGTGCTTCATGCCACTGCCTATTTTCTCTCCAAGGAGAGGATAAAGGAAACT
  TTAGATCCAATTGATGAGGTCGCTGCACTCATCGCAGCCACCATTCATGATGTGGATCAC
  CCTGGGAGAACCAACTCCTTCCTGTGTAATGCTGGAAGTGAGCTGGCCATTTTGTACAAT
  GACACTGCTGTGCTGGAGAGCCACCATGCGGCCTTGGCCTTCCAGCTGACCACTGGAGAT
  GATAAATGCAATATATTTAAAAACATGGAGAGGAATGATTATCGGACACTGCGCCAGGGG
  ATTATCGACATGGTCTTAGCCACAGAAATGACAAAGCACTTTGAGCATGTCAACAAATTT
  GTCAACAGCATCAACAAACCCTTGGCAACACTAGAAGAAAATGGGGAAACTGATAAAAAC
  CAGGAAGTGATAAACACTATGCTTAGGACTCCAGAGAACCGGACCCTAATCAAACGAATG
  CTGATTAAATGTGCTGATGTGTCCAATCCCTGCCGACCCCTGCAGTACTGCATCGAGTGG
  GCTGCACGCATTTCGGAAGAATATTTTTCTCAGACTGATGAAGAGAAGCAGCAGGGCTTA
  CCTGTGGTGATGCCAGTGTTTGACAGAAATACCTGCAGCATCCCCAAATCCCAAATCTCT
  TTCATTGATTACTTCATCACAGACATGTTTGATGCTTGGGATGCCTTTGTAGACCTGCCT
  GATTTAATGCAGCATCTTGACAACAACTTTAAATACTGGAAAGGACTGGACGAAATGAAG
  CTGCGGAACCTCCGACCACCTCCTGAATAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002605 unedited
  ATACGACTCACTATAGGGCGGCCGCGATTTGGCACGAGGCATGTTCGGAGGGGCGGCCTC
  GGCACGCCACCCGCCTAAGCGCCCCCTTCCCACCGCAGCCGCCGCCGCCGCAGCGCCCGC
  ACCGCGATAAAAGGGGCGGCCGCGTTTCCTGACGCGAGATCCGCGCTCGCCGCCGCCCGC
  ACAGGCGGCGATGACACGGCGCCCGCGGCGGCCCGGAGGCGCCGGGTGGGCCGTTTGCTG
  ACCGGATCGCGGCTACCCGCCAGCGTGTCCGCGGCGCCGCCGCCAGCATGGGCTGTGCCC
  CGAGCATCCACATTTCCGAGCGCCTGGTGGCCGAGGACGCGCCTAGCCCCGCGGCACCGC
  CGCTGTCGTCCGGCGGGCCGCGCCTCCCGCAGGGCCAGAAGACGGCCGCCTTGCCCCGGA
  CCCGCGGCGCCGGCCTCTTGGAGTCGGAGCTTCGCGACGGCAGCGGCAAGAAGGTAGCAG
  TAGCTGATGTGCAGTTTGGCCCCATGAGATTTCATCAAGATCAACTTCAGGTACTTTTAG
  TGTTTACCAAAGAAGATAACCAATGTAATGGATTCTGCAGGGCATGTGAAAAAGCAGGGT
  TTAAGTGTACAGTTACCAAGGAGGCTCAGGCTGTCCTTGCCTGTTTCCTGGACANACATC
  ATGACATTATCATCATAGACCACAGAAATCCTCGACAGCTGGATGCAGAGGCACTGTGCA
  GGTCTATCAGATCATCAAAACTCTCAGAAACACAGTTATTGTTGGTGTACTACGCAGGGT
  GGATAGAAAGAAGTGTCCCGTATGCCTTTCATTCTGCTGGATTACAGGNAGGATGTAGAA
  AACCCCACATCATGGCCTGCTACACTGACTGCTCCACTTGAGTTTGGAGANTGCCATCAC
  ACTGAACTCAGGCTTGTACTCATATTCCTGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002605 unedited
  GGGGAGAGCAGGGGGNNNNCNGCNANCCTCCCATCCCTGTTTTTGACTCTGCACNCGCGG
  CCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTGATATTAATAATCAAGTT
  CAAATTTTAATTTTTCATCTGCTACTCAATTTTAAAAGAGAAGTCACTCTCAGTAGTGTT
  CCCAAGTGAGCGATTGCACCTTCTATGATGTGAAAAAGTTGCTAAAGATCCTATTGCCAT
  TAGCCGTTCTTAAGTGCTGAACGTAACTGAGAGAATTGTTTGCAATAGACATGCTCTGTC
  ACTGGCAGGAAGTCCATCAATACCCTTTGCAGGGGCACTCCAGACCTTGTGTTTTACCAG
  AAACTGAGGCAAACCGTGGTCCTCATGTGCCTTTCTGAAGAGCTCCTGCAGCAGGCCCAG
  GCTACAGTGGCCAAGTCTCACTTGACAGTAATGGCATTGGATGGTGGCTGCAGAAGGTTA
  CTGATGTCCCCGTGGGCTTTCACAGGCTATGCAGATCGGGGGCTGTTCTGCCTAGTACTG
  AAAGGACCAAGGAGCTCTGGCTGCCCTCAGGAATTCCAAATGACCGAAGGAACAAAGCTT
  CAGGGCTCTGGGTGGTGCCTCCCACTATTCAGCAGGTGGTCGGAGGTTCCGCAGCTTCAT
  TTCGTCCAGTCCTTTCCAGTATTTAAAGTTGTTGTCAAGATGCTGCATTACACCACGCAG
  GTCTACAAAGGCATTCCCAGCATCAAACATGTCTCGTGATGACGTAATCAATGAAAGAGA
  ATTGGGATTTGGGGATGCTGCAGGTATTTCTGTCAAACACTGCATCACCCCAGGAAAGCC
  CTGCTGCTTCTTTTCTCCACCTGAGAAAAAATTCTTCCGAAAGCGTGCACCCCACTCGTG
  CCGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002605
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002605.2, NP_002596.1
• RefSeq Size: 3931 bp
• RefSeq ORF: 2490 bp
• Locus ID: 5151
• Cytogenetics: 15q25.3
• Domains: PDEase
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: 'The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]'
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longest isoform (1).