CYBA (NM_000101) Human Untagged Clone

CAT#: SC101113

CYBA (untagged)-Human cytochrome b-245, alpha polypeptide (CYBA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYBA
• Synonyms: CGD4; p22-PHOX
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC101113 sequence for NM_000101 edited (data generated by NextGen Sequencing)
  ATGGGGCAGATCGAGTGGGCCATGTGGGCCAACGAGCAGGCGCTGGCGTCCGGCCTGATC
  CTCATCACCGGGGGCATCGTGGCCACAGCTGGGCGCTTCACCCAGTGGTACTTTGGTGCC
  TACTCCATTGTGGCGGGCGTGTTTGTGTGCCTGCTGGAGTACCCCCGGGGGAAGAGGAAG
  AAGGGCTCCACCATGGAGCGCTGGGGATAGAAGCACATGACCGCCGTGGTGAAGCTGTTC
  GGGCCCTTTACCAGGAATTACTATGTTCGGGCCGTCCTGCATCTCCTGCTCTCGGTGCCC
  GCCGGCTTCCTGCTGGCCACCATCCTTGGGACCGCCTGCCTGGCCATTGCGAGCGGCATC
  TACCTACTGGCGGCTGTGCGTGGCGAGCAGTGGACGCCCATCGAGCCCAAGCCCCGGGAG
  CGGCCGCAGATCGGAGGCACCATCAAGCAGCCGCCCAGCAACCCCCCGCCGCGGCCCCCG
  GCCGAGGCCCGCAAGAAGCCCAGCGAGGAGGAGGCTGCGGTGGCGGCGGGGGGACCCCCG
  GGAGGTCCCCAGGTCAACCCCATCCCGGTGACCGACGAGGTCGTGTGA
  
  Clone variation with respect to NM_000101.2
  36 a=>g;208 c=>t;214 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000101 unedited
  GGTCAAAATTTGTAAACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCAGTGTCCC
  AGCCGGGTTCGTGTCGCCTGGGGCAGATCGAGTGGGCCATGTGGGCCAACGAGCAGGCGC
  TGGCGTCCGGCCTGATCCTCATCACCGGGGGCATCGTGGCCACAGCTGGGCGCTTCACCC
  AGTGGTACTTTGGTGCCTACTCCATTGTGGCGGGCGTGTTTGTGTGCCTGCTGGAGTACC
  CCCGGGGGAAGAGGAAGAAGGGCTCCACCATGGAGCGCTGGGGATAGAAGCACATGACCG
  CCGTGGTGAAGCTGTTCGGGCCCTTTACCAGGAATTACTATGTTCGGGCCGTCCTGCATC
  TCCTGCTCTCGGTGCCCGCCGGCTTCCTGCTGGCCACCATCCTTGGGACCGCCTGCCTGG
  CCATTGCGAGCGGCATCTACCTACTGGCGGCTGTGCGTGGCGAGCAGTGGACGCCCATCG
  AGCCCAAGCCCCGGGAGCGGCCGCAGATCGGAGGCACCATCAAGCAGCCGCCCAGCAACC
  CCCCGCCGCGGCCCCCGGCCGAGGCCCGCAAGAAGCCCAGCGAGGAGGAAGCTGCGGTGG
  CGGCNGGGGGGGACCCCCCGGAGGTCCCCAGGTCAACCCCATCCCGGTGACCGACGAGGT
  CGTGTGACCTCGNCCGGNACCTGCCCTCCGCCAGGGTGCACCACCTGCAATAATGCAGCG
  AAGCCGGNNAAAAAAANNNAAANAAAAAANANNNNAAAAAAAAAAAAAAAANCCTCGATT
  CTAGATTGCGGCCGGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATTCCTGTGAC
  CCCTCCCCATGGCTTCTCTGGCCCTGGAAGTTGCCACTCAGGTGCCACCACCCTTGCCCT
  AATAAATTTAGTGGCATCATTTTGTCTGACTAGGTGTCCTTCTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000101 unedited
  AGCCGCATTTGAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTCCGGCTTCGTTGCATTTATTGCAGGGGGGTGCACCTGGCGGGAGGGC
  AGGTCCGGGGCAAGGTCACACAACCTCGTCGGTCACCGGGATGGGGTTGACCTGGGGACC
  TCCCGGGGGTCCCCCCGCCGCCACCGAAGCCTCCTCCTCGTTGGGCTTTTTGGGGGCCTC
  GGCCGGGGGCCGGGGGGGGGGGTTGCTGGGCGGTTGTTTGATGGGGCCTCCAATCTGGGG
  CCGTTCCCGGGGTTTGGGCTCAATGGGCGTCCACTGTTCGCCACGCACAGCCGCCAGTAG
  GTAAATGCCGTTCGCAATGGCCAGCCAGGCGGTCCCAAGGATGGGGGCCAACAGGAACCC
  GGGGGCCACCGAAAGCAGGAAATGCAGGACGGCCCAAACATAGTAATTCCTGGTAAAGGG
  CCCGAACAGTTTCACCACGGGGGTCATGTGCTTCTATCCCCACCGCTCCATGGGGGAGCC
  CTTTTTTCTTTTCCCCCGGGGGTACTCCAGCAGGCACACAAACACGCCCGCCACAATGGA
  GTAGGCACCAAATTACCACTGGGTGAAGCGCCCAGCTGTGGCCACAATGCCCTCGGTGAT
  GAGGATAAAGCCGGACCCAACGCCTGTTTTTTGGCCCACATGGCCATTTATTTGCCCCAT
  GCGAAACCAACCGGGTGGAAACTGCCTGTGCCGAATTTGGGCCGCCCTAAGGGAGTCGAT
  TAAAAATTCTGCGGGTCACTAAAAAGCTTTGTTTATTATAACTNCCACGTAACCCCTTAC
  GCTATTGGGTAACGGGCCGGGGTTTACCAATTTTGAAAGCCCGTGATTTTGGGCCAAACA
  ACTCCATTGACGCAAGGGGGGAAACTTGAAATCCCCGTGGTCACCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000101
• Insert Size: 590 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000101.2, NP_000092.2
• RefSeq Size: 743 bp
• RefSeq ORF: 588 bp
• Locus ID: 1535
• Cytogenetics: 16q24.2
• Domains: cytochr_b558a
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Leukocyte transendothelial migration
• Gene Summary: 'Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light, alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD), that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells. [provided by RefSeq, Jul 2008]'