MRPL27 (NM_148571) Human Untagged Clone

CAT#: SC101195

MRPL27 (untagged)-Human mitochondrial ribosomal protein L27 (MRPL27), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRPL27
• Synonyms: L27mt; L27mt, MGC23716; MGC23716; mitochondrial ribosomal protein L27
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_148571, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGTCGGTGGTGTTGGCGCTGAGGACCCGGACAGCCGTTACATCCTTGCTAAGCCCCACTCCGGCTA
  CAGCTCTTGCTGTCAGATACGCATCCAAGAAGTCGGGTGGTAGCTCCAAAAACCTCGGTGGAAAGTCATC
  AGGCAGACGCCAAGGCATTAAGAAAATGGAAGGTCACTATGTTCATGCTGGGAACATCATTGCAACACAG
  CGCCATTTCCGCTGGCACCCAGGTGCCCATGTGAGTTGCTCCGTTGCTGCCCCCCTTTTTCCTTTTCTAG
  GTTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_148571 unedited
  ACTGACTATGGAGTNNNNNNNNNNNNNNNAGAGGGTGATCGAAAGCTGGCGTCGGCGGTG
  TTGGCGCTGAGGACCCGGACAGCCGTTACATCCTTGCTAAGCCCCACTCCGGCTACAGCT
  CTTGCTGTCAGATACGCATCCAAGAAGTCGGGTGGTAGCTCCAAAAACCTCGGTGGAAAG
  TCATCAGGCAGACGCCAAGGCATTAAGAAAATGGAAGGTCACTATGTTCATGCTGGGAAC
  ATCATTGCAACACAGCGCCATTTCCGCTGGCACCCAGGTGCCCATGTGAGTTGCTCCGTT
  GCTGCCCCCCTTTTTCCTTTTCTAGGTTGACCTCTCCTTGCCCCTAAGCATGGTAATAAC
  AGTTGCATGTATTGAGTGCTTACCAAATGGCAAGCATTGTGCTGATTCCCATGCCTACAC
  GATCTCATTTCTTCCTTACCACATCCCTGNAAGTAAGGTGAAATGCCANAGACCTANACG
  GNGTGGAAGGAGCAAGTGACTGCTGGGATTTGCACCANGTCTGCCTAACTCCCAGATCAC
  TATGATTTGCCCTGGTGTTGCATTGGCCTGGTATTCTTGGGTCTCCTTTCTAACCCTCAG
  TTCTTGGAGGACAAGATACCTGGTGATTTAAAATATTATTTTAGTCTGGGAACTAAATTC
  ATTATTAGTNNTCATATTCTAAGACTCTTNCTTGTATAACTACTGCAGATGGTGAANGAT
  ACTTGATTAATGAATAGAATCAGTTGCCAGAGGTAGTTATACCAGTGGGATTCAGTTCAG
  CAGTTGCATATTTTATGGGANGACAGTTTTGTACAAACTACGAACTGACTCTCCATATTT
  AAATATAGTGCTTGNACAGTTTCCTCCCACCCTTTCATATTTATAGACAAAGGTGATAGA
  CTGTTTATCAGAGCTATNGATGGAATGTTGATGAGACTTTACTTAACTACATTAAGGGGC
  TTTACAATATCACTACATTGCTNAGTTTAGCCTTGATGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_148571
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_148571.1, NP_683412.1
• RefSeq Size: 2472 bp
• RefSeq ORF: 285 bp
• Locus ID: 51264
• Cytogenetics: 17q21.33
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1, that results in a frameshift. It encodes isoform b which has a shorter and distinct C-terminus compared to isoform a.