PIGK (NM_005482) Human Untagged Clone

CAT#: SC101219

PIGK (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class K (PIGK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIGK
• Synonyms: GPI8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC101219 sequence for NM_005482 edited (data generated by NextGen Sequencing)
  ATGGCCGTCACCGACAGCCTCAGCCGGGCTGCGACTGTCTTGGCAACTGTGTTGCTCTTG
  TCCTTCGGCAGCGTGGCCGCTAGTCATATCGAGGATCAAGCAGAACAATTCTTTAGAAGT
  GGCCATACAAACAACTGGGCTGTTCTGGTGTGTACATCCCGATTCTGGTTTAATTATCGA
  CATGTTGCAAATACCCTTTCTGTTTATAGAAGTGTCAAGAGGCTAGGTATTCCTGACAGT
  CACATTGTCCTAATGCTTGCAGATGATATGGCCTGTAATCCTAGAAATCCCAAACCAGCT
  ACAGTGTTTAGTCACAAGAATATGGAACTAAATGTGTATGGAGATGATGTGGAAGTGGAT
  TATAGAAGTTATGAGGTAACTGTGGAGAATTTTTTACGGGTATTAACTGGGAGGATCCCA
  CCTAGTACTCCTCGGTCAAAACGTCTTCTTTCTGATGACAGAAGCAATATTCTAATTTAT
  ATGACAGGGCATGGTGGAAATGGTTTCTTAAAATTTCAAGATTCTGAAGAAATTACCAAC
  ATAGAACTCGCGGATGCTTTTGAACAAATGTGGCAGAAAAGACGCTACAATGAGCTACTG
  TTTATTATTGATACTTGCCAAGGAGCATCCATGTATGAACGATTTTATTCTCCTAACATA
  ATGGCTCTAGCTAGTAGTCAAGTGGGAGAAGATTCACTCTCGCATCAACCTGATCCTGCA
  ATTGGAGTCCATCTTATGGATAGATACACATTTTATGTCTTGGAATTTTTGGAAGAAATT
  AACCCAGCTAGCCAAACTAATATGAATGACCTTTTTCAGGTATGTCCCAAAAGTCTGTGT
  GTGTCTACTCCTGGACATCGCACTGATCTTTTTCAGAGGGATCCTAAAAATGTACTGATA
  ACTGATTTCTTTGGAAGTGTACGGAAAGTGGAAATTACAACAGAGACTATTAAATTGCAA
  CAGGATTCAGAAATCATGGAAAGCAGCTATAAGGAAGACCAGATGGATGAGAAACTAATG
  GAACCTCTGAAATATGCTGAACAACTTCCTGTAGCTCAGATAATACACCAGAAACCGAAG
  CTGAAAGACTGGCATCCTCCTGGGGGCTTTATTCTGGGATTATGGGCACTTATTATCATG
  GTTTTCTTCAAAACTTATGGAATTAAGCATATGAAGTTCATTTTTTAG
  
  Clone variation with respect to NM_005482.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005482 unedited
  GTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGTGAAGCCGGTA
  AACATGGCCGTCACCGACAGCCTCAGCCGGGCTGCGACTGTCTTGGCAACTGTGTTGCTC
  TTGTCCTTCGGCAGCGTGGCCGCTAGTCATATCGAGGATCAAGCAGAACAATTCTTTAGA
  AGTGGCCATACAAACAACTGGGCTGTTCTGGTGTGTACATCCCGATTCTGGTTTAATTAT
  CGACATGTTGCAAATACCCTTTCTGTTTATAGAAGTGTCAAGAGGCTAGGTATTCCTGAC
  AGTCACATTGTCCTAATGCTTGCAGATGATATGGCCTGTAATCCTAGAAATCCCAAACCA
  GCTACAGTGTTTAGTCACAAGAATATGGAACTAAATGTGTATGGAGATGATGTGGAAGTG
  GATTATAGAAGTTATGAGGTAACTGTGGAGAATTTTTTACGGGTATTAACTGGGAGGATC
  CCACCTAGTACTCCTCGGTCAAAACGTCTTCTTTCTGATGACAGAAGCAATATTCTAATT
  TATATGACAGGGCATGGTGGAAATGGTTTCTTAAAATTTCAAGATTCTGAAGAAATTACC
  AACATAGAACTCGCGGATGCTTTTGAACAAATGTGGCAGAAAAGACGCTACAATGAGCTA
  CTGTTTATTATTGATACTTGCCAAGGAGCATCCATGTATGAACGATTTTATTCTCCTAAC
  ATAATGGCTCTAGCTAGTAGTCAAGTGGGAGAAGATTCACTCTCGCATCAACCTGATCCT
  GNCATTGGAGTCCATCTTATGGATAGATACACATTNTATGTCTTGGAATTTTGGNAAGAA
  ATAACCCAGCTAGCCAAACTAATATGAATGACCTTNNTCAGGTATGTCCCANAAGTCTTG
  TGTGTGTCTACTCCTGGCATCGCACTGATCTTTTNCANAGGGATCCTAAAATGTACTGGA
  TACTGATTCTTTTGGAAGGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005482 unedited
  AATTTACTTGNACCGCGGCCCGCTTNCTAGNGATCGATTTTTTTTTTTTTTTTTTCATCT
  TTGCTTTTAATTAAAGCTGCAGGATTCCATGAGTGACCAGCAGTAAAATATTAATACATT
  AAAGAATAAACCAAAGGAAAACATTTGGTACAATGTAAGTGGTCCAACAATAGAAGACTG
  TGAAATGAATTAAATATTCATAAAGTGAATACTATGCAGTCATTCACAATGTATTTTTGA
  GAGGGAAAAGGTTGATAATACATTGTAAAATGAAAAAAGACATTACAAAATAGTACATTC
  CATATGATCTCCATATTGGGAAAAAATATGTGCATAAGTCAAAAGATTGGGAGGATATAC
  AAAAAATAATTAACCAAAGTTACTTCTGTTAGTGAGAATTTAATGTCTTCACAATTGTTT
  CTTTTCCAAATGAAAAGGGGATAAGGGGAAATTACTATTTTCAAAAACAGTCAATGTATT
  CTGATCTCTGTCTCATTCGCTTTTATAATACACAAAATACAAAACTGGAATATATTTAGT
  GCCATTAAGCAGTTGCATTAACAATTATTTTTCTTTAAGTTATAAAATTAATAGTTGATT
  CAAATTTAGTTTCCTTATACTTATTTCCAATTCATACAAGAGAAACACATTTTTAAAAAT
  ATATAATGACATAAATTATTATCCAAGTTTGCAGTCCCTCATGCATTCTTCATTCATCAT
  CAACTCTAAAAAATGAACTTCATATGCTTAATTCCATAAGTTTTGAAGAAAACCATGATA
  ATAAGTGCCCATAATCCCAGAATAAAGCCCCCAGGAGGATGCCAGTCTTTCAGCTTCGGT
  TTCTGGTGTATTATCTGAGCTACAGGAAGTTTGTCCACCATATTTCAGAGTTTCCCATAG
  TTTCCTCACCCATCTGGGTCTTCCTTATAGCTGCTTTTCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005482
• ORF Size: 1188 bp
• Insert Size: 2000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: A TrueClone.

Reference Data

• RefSeq: NM_005482.1, NP_005473.1
• RefSeq Size: 1897
• RefSeq ORF: 1188
• Locus ID: 10026
• Domains: Peptidase_C13
• Protein Families: Druggable Genome, Protease, Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
• Gene Summary: This gene encodes a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. [provided by RefSeq, Jul 2008]