PIGK (NM_005482) Human Tagged ORF Clone

CAT#: RG204728

PIGK (GFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class K (PIGK)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PIGK
• Synonyms: GPI8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204728 representing NM_005482
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTCACCGACAGCCTCAGCCGGGCTGCGACTGTCTTGGCAACTGTGTTGCTCTTGTCCTTCGGCA
  GCGTGGCCGCTAGTCATATCGAGGATCAAGCAGAACAATTCTTTAGAAGTGGCCATACAAACAACTGGGC
  TGTTCTGGTGTGTACATCCCGATTCTGGTTTAATTATCGACATGTTGCAAATACCCTTTCTGTTTATAGA
  AGTGTCAAGAGGCTAGGTATTCCTGACAGTCACATTGTCCTAATGCTTGCAGATGATATGGCCTGTAATC
  CTAGAAATCCCAAACCAGCTACAGTGTTTAGTCACAAGAATATGGAACTAAATGTGTATGGAGATGATGT
  GGAAGTGGATTATAGAAGTTATGAGGTAACTGTGGAGAATTTTTTACGGGTATTAACTGGGAGGATCCCA
  CCTAGTACTCCTCGGTCAAAACGTCTTCTTTCTGATGACAGAAGCAATATTCTAATTTATATGACAGGGC
  ATGGTGGAAATGGTTTCTTAAAATTTCAAGATTCTGAAGAAATTACCAACATAGAACTCGCGGATGCTTT
  TGAACAAATGTGGCAGAAAAGACGCTACAATGAGCTACTGTTTATTATTGATACTTGCCAAGGAGCATCC
  ATGTATGAACGATTTTATTCTCCTAACATAATGGCTCTAGCTAGTAGTCAAGTGGGAGAAGATTCACTCT
  CGCATCAACCTGATCCTGCAATTGGAGTCCATCTTATGGATAGATACACATTTTATGTCTTGGAATTTTT
  GGAAGAAATTAACCCAGCTAGCCAAACTAATATGAATGACCTTTTTCAGGTATGTCCCAAAAGTCTGTGT
  GTGTCTACTCCTGGACATCGCACTGATCTTTTTCAGAGGGATCCTAAAAATGTACTGATAACTGATTTCT
  TTGGAAGTGTACGGAAAGTGGAAATTACAACAGAGACTATTAAATTGCAACAGGATTCAGAAATCATGGA
  AAGCAGCTATAAGGAAGACCAGATGGATGAGAAACTAATGGAACCTCTGAAATATGCTGAACAACTTCCT
  GTAGCTCAGATAATACACCAGAAACCGAAGCTGAAAGACTGGCATCCTCCTGGGGGCTTTATTCTGGGAT
  TATGGGCACTTATTATCATGGTTTTCTTCAAAACTTATGGAATTAAGCATATGAAGTTCATTTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204728 representing NM_005482
  Red=Cloning site Green=Tags(s)
  
  MAVTDSLSRAATVLATVLLLSFGSVAASHIEDQAEQFFRSGHTNNWAVLVCTSRFWFNYRHVANTLSVYR
  SVKRLGIPDSHIVLMLADDMACNPRNPKPATVFSHKNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRIP
  PSTPRSKRLLSDDRSNILIYMTGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFIIDTCQGAS
  MYERFYSPNIMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFLEEINPASQTNMNDLFQVCPKSLC
  VSTPGHRTDLFQRDPKNVLITDFFGSVRKVEITTETIKLQQDSEIMESSYKEDQMDEKLMEPLKYAEQLP
  VAQIIHQKPKLKDWHPPGGFILGLWALIIMVFFKTYGIKHMKFIF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005482
• ORF Size: 1185 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_005482.2, NP_005473.1
• RefSeq Size: 4626
• RefSeq ORF: 1188
• Locus ID: 10026
• Domains: Peptidase_C13
• Protein Families: Druggable Genome, Protease, Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
• Gene Summary: This gene encodes a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. [provided by RefSeq, Jul 2008]