PSMB8 (NM_148919) Human Untagged Clone

CAT#: SC101220

PSMB8 (untagged)-Human proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7) (PSMB8), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMB8
• Synonyms: ALDD; D6S216; D6S216E; JMP; LMP7; NKJO; PRAAS1; PSMB5i; RING10
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC101220 sequence for NM_148919 edited (data generated by NextGen Sequencing)
  ATGGCGCTACTAGATGTATGCGGAGCCCCCCGAGGGCAGCGGCCGGAATCGGCTCTCCCG
  GTTGCGGGAAGCGGGCGTCGCTCGGACCCAGGACACTACAGTTTCTCTATGCGATCTCCA
  GAGCTCGCTTTACCCCGGGGAATGCAGCCCACAGAATTCTTCCAGTCCCTGGGTGGGGAC
  GGAGAAAGGAACGTTCAGATTGAGATGGCCCATGGCACCACCACGCTCGCCTTCAAGTTC
  CAGCATGGAGTGATTGCAGCAGTGGATTCTCGGGCCTCAGCTGGGTCCTACATTAGTGCC
  TTACGGGTGAACAAGGTGATTGAGATTAACCCTTACCTGCTTGGCACCATGTCTGGCTGT
  GCAGCAGACTGTCAGTACTGGGAGCGCCTGCTGGCCAAGGAATGCAGGCTGTACTATCTG
  CGAAATGGAGAACGTATTTCAGTGTCGGCAGCCTCCAAGCTGCTGTCCAACATGATGTGC
  CAGTACCGGGGCATGGGCCTCTCTATGGGCAGTATGATCTGTGGCTGGGATAAGAAGGGT
  CCTGGACTCTACTACGTGGATGAACATGGGACTCGGCTCTCAGGAAATATGTTCTCCACG
  GGTAGTGGGAACACTTATGCCTACGGGGTCATGGACAGTGGCTATCGGCCTAATCTTAGC
  CCTGAAGAGGCCTATGACCTTGGCCGCAGGGCTATTGCTTATGCCACTCACAGAGACAGC
  TATTCTGGAGGCGTTGTCAATATGTACCACATGAAGGAAGATGGTTGGGTGAAAGTAGAA
  AGTACAGATGTCAGTGACCTGCTGCACCAGTACCGGGAAGCCAATCAATAA
  
  Clone variation with respect to NM_148919.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_148919 unedited
  GTCAGAAATTTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGCTGGGC
  GGTCATGGCGCTACTAGATGTATGCGGAGCCCCCCGAGGGCAGCGGCCGGAATCGGCTCT
  CCCGGTTGCGGGAAGCGGGCGTCGCTCGGACCCAGGACACTACAGTTTCTCTATGCGATC
  TCCAGAGCTCGCTTTACCCCGGGGAATGCAGCCCACAGAATTCTTCCAGTCCCTGGGTGG
  GGACGGAGAAAGGAACGTTCAGATTGAGATGGCCCATGGCACCACCACGCTCGCCTTCAA
  GTTCCAGCATGGAGTGATTGCAGCAGTGGATTCTCGGGCCTCAGCTGGGTCCTACATTAG
  TGCCTTACGGGTGAACAAGGTGATTGAGATTAACCCTTACCTGCTTGGCACCATGTCTGG
  CTGTGCAGCAGACTGTCAGTACTGGGAGCGCCTGCTGGCCAAGGAATGCAGGCTGTACTA
  TCTGCGAAATGGAGAACGTATTTCAGTGTCGGCAGCCTCCAAGCTGCTGTCCAACATGAT
  GTGCCAGTACCGGGGCATGGGCCTCTCTATGGGCAGTATGATCTGTGGCTGGGATAAGAA
  GGGTCCTGGACTCTACTACGTGGATGAACATGGGACTCGGCTCTCAGGAAATATGTTCTC
  CACGGGTAGTGGGAACACTTATGCCTACGGGGTCATGGACAGTGGCTATCGGCCTAATCT
  TAGCCCTGAAGAGGCCTATGACCTTGGCCGCAGGGCTATTGCTTATGCCACTCACAGAGA
  CAGCTATTCTGGGAGGCGTTGTCAATATGTACCACATGAAGGAAAGAATGGTTGGGTTGA
  AAAGTANAAAGTTACAGAATGTCAGTTGACCTGCTGCACCAGTACCGGGGAGCCATCATA
  TGGGTGGGTGGGTGCAGCTGGGCAGGTCTCTCTGGGGAGGTCTTGGCCGACTCAGGGACC
  TAAGCACGTTTAGTCCAAGGAAAAAAAAGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_148919 unedited
  AAGGCTCCTGGCTGTAACTTCAGGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGC
  CACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTT
  TTTTTTTTTTTTTTTTTTTATTTATAACCGTTTTTCTTTATTCTACTTAGTGGGGCACCC
  AGAAACTTCCCTGGGGGAAATGCTTGTTCAAATAGAGAACACGCAGAAGATGCACTTCAC
  CGGCCTCCTCTGGCTGCTGAGCCCGTACTCTCTCTTTGGCTCAGGCTAGGCCTCTTCTTC
  TCCTTGGACTTAACGTGGCTTAGGTCCCTGAGTCGGCCAAGACCTCCCAGAGGAGACCTG
  CCCAGCTGCCACCACCACCATTATTGATTGGCTTCCCGGTACTGGTGCAGCAGGTCACTG
  ACATCTGTACTTTCTACTTTCACCCAACCATCTTCCTTCATGTGGTACATATTGGACAAC
  GCCTCCAGAATAGCTGTCTCTGTGAGTGGCATAAGCAATAGCCCTGCGGCCAAGGTCATA
  GGCCTCTTCAGGGCTAAGATTAGGCCGATAGCCACTGTCCATGACCCCGTAGGCATAAGT
  GTTCCCACTACCCGTGGAGAACATATTTTCTGAGAGCCGAGTCCCATGTTCATCCACGTA
  GTAGAGTCCAAGACCCTTTCTTATCCNAGCCACAGATCATACTTGCCATAGAGAAGGCCA
  TGCCCCGTACTTGCACAATCATGTGGACAAGACTTGGGGCTTGCGACCTGAATACTTCTC
  ATTCGGAAATAGTCACTTGATTCCTGGCAAAGGGTTTCATACTTGAGTGTTGCAACCAAT
  GGCAACGGTAAGTATTCATACACTTGTCCGAGAGCTTGTGGCCCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_148919
• Insert Size: 1150 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_148919.3, NP_683720.2
• RefSeq Size: 1135 bp
• RefSeq ORF: 831 bp
• Locus ID: 5696
• Cytogenetics: 6p21.32
• Domains: proteasome
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome
• Gene Summary: 'The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR and contains an alternate in-frame exon in the 5' coding region, compared to variant 1. Isoform E2 has a distinct N-terminus, compared to isoform E1.