CCK4 (PTK7) (NM_152880) Human Untagged Clone

CAT#: SC101238

PTK7 (untagged)-Human PTK7 protein tyrosine kinase 7 (PTK7), transcript variant PTK7-2


  "NM_152880" in other vectors (5)

Reconstitution Protocol

USD 2,100.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PTK7
Synonyms CCK-4; CCK4
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC101238 sequence for NM_152880 edited (data generated by NextGen Sequencing)
ATGGGAGCTGCGCGGGGATCCCCGGCCAGACCCCGCCGGTTGCCTCTGCTCAGCGTCCTG
CTGCTGCCGCTGCTGGGCGGTACCCAGACAGCCATTGTCTTCATCAAGCAGCCGTCCTCC
CAGGATGCACTGCAGGGGCGCCGGGCGCTGCTTCGCTGTGAGGTTGAGGCTCCGGGCCCG
GTACATGTGTACTGGCTGCTCGATGGGGCCCCTGTCCAGGACACGGAGCGGCGTTTCGCC
CAGGGCAGCAGCCTGAGCTTTGCAGCTGTGGACCGGCTGCAGGACTCTGGCACCTTCCAG
TGTGTGGCTCGGGATGATGTCACTGGAGAAGAAGCCCGCAGTGCCAACGCCTCCTTCAAC
ATCAAATGGATTGAGGCAGGTCCTGTGGTCCTGAAGCATCCAGCCTCGGAAGCTGAGATC
CAGCCACAGACCCAGGTCACACTTCGTTGCCACATTGATGGGCACCCTCGGCCCACCTAC
CAATGGTTCCGAGATGGGACCCCCCTTTCTGATGGTCAGAGCAACCACACAGTCAGCAGC
AAGGAGCGGAACCTGACGCTCCGGCCAGCTGGTCCTGAGCATAGTGGGCTGTATTCCTGC
TGCGCCCACAGTGCTTTTGGCCAGGCTTGCAGCAGCCAGAACTTCACCTTGAGCATTGCT
GATGAAAGCTTTGCCAGGGTGGTGCTGGCACCCCAGGACGTGGTAGTAGCGAGGTATGAG
GAGGCCATGTTCCATTGCCAGTTCTCAGCCCAGCCACCCCCGAGCCTGCAGTGGCTCTTT
GAGGATGAGACTCCCATCACTAACCGCAGTCGCCCCCCACACCTCCGCAGAGCCACAGTG
TTTGCCAACGGGTCTCTGCTGCTGACCCAGGTCCGGCCACGCAATGCAGGGATCTACCGC
TGCATTGGCCAGGGGCAGAGGGGCCCACCCATCATCCTGGAAGCCACACTTCACCTAGCA
GAGATTGAAGACATGCCGCTATTTGAGCCACGGGTGTTTACAGCTGGCAGCGAGGAGCGT
GTGACCTGCCTTCCCCCCAAGGGTCTGCCAGAGCCCAGCGTGTGGTGGGAGCACGCGGGA
GTCCGGCTGCCCACCCATGGCAGGGTCTACCAGAAGGGCCACGAGCTGGTGTTGGCCAAT
ATTGCTGAAAGTGATGCTGGTGTCTACACCTGCCACGCGGCCAACCTGGCTGGTCAGCGG
AGACAGGATGTCAACATCACTGTGGCCACTGTGCCCTCCTGGCTGAAGAAGCCCCAAGAC
AGCCAGCTGGAGGAGGGCAAACCCGGCTACTTGGATTGCCTGACCCAGGCCACACCAAAA
CCTACAGTTGTCTGGTACAGAAACCAGATGCTCATCTCAGAGGACTCACGGTTCGAGGTC
TTCAAGAATGGGACCTTGCGCATCAACAGCGTGGAGGTGTATGATGGGACATGGTACCGT
TGTATGAGCAGCACCCCAGCCGGCAGCATCGAGGCGCAAGCCCGTGTCCAAGTGCTGGAT
GGGAGCAGCCTCCCAGAGTGGGTGACAGACAACGCTGGGACCCTGCATTTTGCCCGGGTG
ACTCGAGATGACGCTGGCAACTACACTTGCATTGCCTCCAACGGGCCGCAGGGCCAGATT
CGTGCCCATGTCCAGCTCACTGTGGCAGTTTTTATCACCTTCAAAGTGGAACCAGAGCGT
ACGACTGTGTACCAGGGCCACACAGCCCTACTGCAGTGCGAGGCCCAGGGAGACCCCAAG
CCGCTGATTCAGTGGAAAGGCAAGGACCGCATCCTGGACCCCACCAAGCTGGGACCCAGG
ATGCACATCTTCCAGAATGGCTCCCTGGTGATCCATGACGTGGCCCCTGAGGACTCAGGC
CGCTACACCTGCATTGCAGGCAACAGCTGCAACATCAAGCACACGGAGGCCCCCCTCTAT
GTCGTGGACAAGCCTGTGCCGGAGGAGTCGGAGGGCCCTGGCAGCCCTCCCCCCTACAAG
ATGATCCAGACCATTGGGTTGTCGGTGGGTGCCGCTGTGGCCTACATCATTGCCGTGCTG
GGCCTCATGTTCTACTGCAAGAAGCGCTGCAAAGCCAAGCGGCTGCAGAAGCAGCCCGAG
GGCGAGGAGCCAGAGATGGAATGCCTCAACGGTGGGCCTTTGCAGAACGGGCAGCCCTCA
GCAGAGATCCAAGAAGAAGTGGCCTTGACCAGCTTGGGCTCCGGCCCCGCGGCCACCAAC
AAACGCCACAGCACAAGTGATAAGATGCACTTCCCACGGTCTAGCCTGCAGCCCATCACC
ACGCTGGGGAAGAGTGAGTTTGGGGAGGTGTTCCTGGCAAAGGCTCAGGGCTTGGAGGAG
GGAGTGGCAGAGACCCTGGTACTTGTGAAGAGCCTGCAGAGCAAGGATGAGCAGCAGCAG
CTGGACTTCCGGAGGGAGTTGGAGATGTTTGGGAAGCTGAACCACGCCAACGTGGTGCGG
CTCCTGGGGCTGTGCCGGGAGGCTGAGCCCCACTACATGGTGCTGGAATATGTGGATCTG
GGAGACCTCAAGCAGTTCCTGAGGATTTCCAAGAGCAAGGATGAAAAATTGAAGTCACAG
CCCCTCAGCACCAAGCAGAAGGTGGCCCTATGCACCCAGGTAGCCCTGGGCATGGAGCAC
CTGTCCAACAACCGCTTTGTGCATAAGGACTTGGCTGCGCGTAACTGCCTGGTCAGTGCC
CAGAGACAAGTGAAGGTGTCTGCCCTGGGCCTCAGCAAGGATGTGTACAACAGTGAGTAC
TACCACTTCCGCCAGGCCTGGGTGCCGCTGCGCTGGATGTCCCCCGAGGCCATCCTGGAG
GGTGACTTCTCTACCAAGTCTGATGTCTGGGCCTTCGGTGTGCTGATGTGGGAAGTGTTT
ACACATGGAGAGATGCCCCATGGTGGGCAGGCAGATGATGAAGTACTGGCAGATTTGCAG
GCTGGGAAGGCTAGACTTCCTCAGCCCGAGGGCTGCCCTTCCAAACTCTATCGGCTGATG
CAGCGCTGCTGGGCCCTCAGCCCCAAGGACCGGCCCTCCTTCAGTGAGATTGCCAGCGCC
CTGGGAGACAGCACCGTGGACAGCAAGCCGTGA

Clone variation with respect to NM_152880.2
1731 g=>a
>OriGene 5' read for NM_152880 unedited
TTATTACACCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA
AGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC
GCGAATTCGGCACGAGGCCTCAGCTCCTTTTCCTGAGCCCGCCGCGATGGGAGCTGCGCG
GGGATCCCCGGCCAGACCCCGCCGGTTGCCTCTGCTCAGCGTCCTGCTGCTGCCGCTGCT
GGGCGGTACCCAGACAGCCATTGTCTTCATCAAGCAGCCGTCCTCCCAGGATGCACTGCA
GGGGCGCCGGGCGCTGCTTCGCTGTGAGGTTGAGGCTCCGGGCCCGGTACATGTGTACTG
GCTGCTCGATGGGGCCCCTGTCCAGGACACGGAGCGGCGTTTCGCCCAGGGCAGCAGCCT
GAGCTTTGCAGCTGTGGACCGGCTGCAGGACTCTGGCACCTTCCAGTGTGTGGCTCGGGA
TGATGTCACTGGAGAAGAAGCCCGCAGTGCCAACGCCTCCTTCAACATCAAATGGATTGA
GGCAGGTCCTGTGGTCCTGAAGCATCCAGCCTCGGAAGCTGAGATCCAGCCACAGACCCA
GGTCACACTTCGTTGCCACATTGATGGGCACCCTCGGCCCACCTACCAATGGTTCCGAGA
TGGGACCCCCCTTTCTGATGGTCAGAGCAACCACACAGTCAGCAGCAAGGAGCGGGACCT
GACGCTCCGGCCAGCTGGTCCTGAGCATAGTGGGCTGTATTCCTGCTGCGCCCACAGTGC
TTTTGGCCCAGCTTGCAGCAGCCAGAACTTCAACTTGAGCATTGCTGATGAAAGCCTTGC
CCAGGTGGTGCCTGGCCCCCCACGACGGGGTATTATCGAGGTATGAGGAGGCCTGTTCCT
TTGACAGTTTCAGCCAGCCACCCCGGAC
>OriGene 3' read for NM_152880 unedited
NGGGAAATCATGNACCGCGCCGCATTCTANGATCGATTTTTTTTTTTTTTTTTTGTAAAA
AAGCTTATTTATTGAGNACAGCGAGTGTAAAAACAAAAACAAAAACAAAAAAACAAAACA
AACAAAAAACGACAACAATAAAAGTGTGGGGTAAGGATGGCCCACCCTCCTCATCTCCAG
GGCCCACCCCTACCTCCCATGCCCACCCACCCCACGCTGCAATTACATATAAAAGCCGCC
CGTGGTGCATACAAAAAGGGCGGTTTATATAGTGTCAAAAGCTCCTGAAAACTCCTTCAT
CTGCCAGGCACTTAGGATGAGGAAAGGAGAGAAGGGTGGGGAAGACGTGGGTCTGGGTCA
GTGTGCACAAGCCCAGAGTGGGGAGGACTCACTTGCTGTGTCCTCTATAATCCTGGTCTA
GACCCCCAAGTGGGACCAGTGGGCCAAGAGACTCATTAACCCTGTGTGCCCAGAACTTGA
GAATATTAAGGAACTCCCCACAAACCCAGCTCATCCCAAGCCTAGCCCTCCCCAGGCAAA
GTTGGCAGATGAGTGGCAGAGTTGGACCCGGTCAAGGGGAGAAGTTGAAGGCCAGAAATT
GGGGTTACCTGTGGCACCCACACTGTCCCTGATAGAAGAAGAGGTGGCAGGGAAAACTGC
CCAGCTCAAAGCCCTAGTCGCCCAGTTTGGGTCCAAGTCAGCCTTCCAAAAGATGAGGGG
TGAGAAAAGGAGAAAAGCCACGCCCTGCTCAGACCTCAGCAATGCCTGTTGCACTAGGGC
AGGGCCTCAGGCCCCACGAGGACCAGGATCCTGCCCATAAGCTGGAAGCTTTCTCTAGAA
AGTCCCCCCTGGCCAGGCCATCCGAGCGGCTCCCTCCTAAAGGTTTGGGTCCCAGGGGCT
GTTTCC
Restriction Sites NotI-NotI     
ACCN NM_152880
Insert Size 4700 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_152880.2, NP_690619.1
RefSeq Size 4153 bp
RefSeq ORF 3093 bp
Locus ID 5754
Cytogenetics 6p21.1
Domains pkinase, TyrKc, S_TKc, ig, IGc2, IG
Protein Families Druggable Genome, Protein Kinase, Transmembrane
Gene Summary 'This gene encodes a member of the receptor protein tyrosine kinase family of proteins that transduce extracellular signals across the cell membrane. The encoded protein lacks detectable catalytic tyrosine kinase activity, is involved in the Wnt signaling pathway and plays a role in multiple cellular processes including polarity and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]'
Transcript Variant: This variant (PTK7-2) lacks an exon in the coding region, but maintains the reading frame, compared to variant PTK7-1. The encoded isoform (b) is shorter than isoform a.

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