CCK4 (PTK7) (NM_152880) Human Untagged Clone

CAT#: SC101238

PTK7 (untagged)-Human PTK7 protein tyrosine kinase 7 (PTK7), transcript variant PTK7-2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTK7
• Synonyms: CCK-4; CCK4
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC101238 sequence for NM_152880 edited (data generated by NextGen Sequencing)
  ATGGGAGCTGCGCGGGGATCCCCGGCCAGACCCCGCCGGTTGCCTCTGCTCAGCGTCCTG
  CTGCTGCCGCTGCTGGGCGGTACCCAGACAGCCATTGTCTTCATCAAGCAGCCGTCCTCC
  CAGGATGCACTGCAGGGGCGCCGGGCGCTGCTTCGCTGTGAGGTTGAGGCTCCGGGCCCG
  GTACATGTGTACTGGCTGCTCGATGGGGCCCCTGTCCAGGACACGGAGCGGCGTTTCGCC
  CAGGGCAGCAGCCTGAGCTTTGCAGCTGTGGACCGGCTGCAGGACTCTGGCACCTTCCAG
  TGTGTGGCTCGGGATGATGTCACTGGAGAAGAAGCCCGCAGTGCCAACGCCTCCTTCAAC
  ATCAAATGGATTGAGGCAGGTCCTGTGGTCCTGAAGCATCCAGCCTCGGAAGCTGAGATC
  CAGCCACAGACCCAGGTCACACTTCGTTGCCACATTGATGGGCACCCTCGGCCCACCTAC
  CAATGGTTCCGAGATGGGACCCCCCTTTCTGATGGTCAGAGCAACCACACAGTCAGCAGC
  AAGGAGCGGAACCTGACGCTCCGGCCAGCTGGTCCTGAGCATAGTGGGCTGTATTCCTGC
  TGCGCCCACAGTGCTTTTGGCCAGGCTTGCAGCAGCCAGAACTTCACCTTGAGCATTGCT
  GATGAAAGCTTTGCCAGGGTGGTGCTGGCACCCCAGGACGTGGTAGTAGCGAGGTATGAG
  GAGGCCATGTTCCATTGCCAGTTCTCAGCCCAGCCACCCCCGAGCCTGCAGTGGCTCTTT
  GAGGATGAGACTCCCATCACTAACCGCAGTCGCCCCCCACACCTCCGCAGAGCCACAGTG
  TTTGCCAACGGGTCTCTGCTGCTGACCCAGGTCCGGCCACGCAATGCAGGGATCTACCGC
  TGCATTGGCCAGGGGCAGAGGGGCCCACCCATCATCCTGGAAGCCACACTTCACCTAGCA
  GAGATTGAAGACATGCCGCTATTTGAGCCACGGGTGTTTACAGCTGGCAGCGAGGAGCGT
  GTGACCTGCCTTCCCCCCAAGGGTCTGCCAGAGCCCAGCGTGTGGTGGGAGCACGCGGGA
  GTCCGGCTGCCCACCCATGGCAGGGTCTACCAGAAGGGCCACGAGCTGGTGTTGGCCAAT
  ATTGCTGAAAGTGATGCTGGTGTCTACACCTGCCACGCGGCCAACCTGGCTGGTCAGCGG
  AGACAGGATGTCAACATCACTGTGGCCACTGTGCCCTCCTGGCTGAAGAAGCCCCAAGAC
  AGCCAGCTGGAGGAGGGCAAACCCGGCTACTTGGATTGCCTGACCCAGGCCACACCAAAA
  CCTACAGTTGTCTGGTACAGAAACCAGATGCTCATCTCAGAGGACTCACGGTTCGAGGTC
  TTCAAGAATGGGACCTTGCGCATCAACAGCGTGGAGGTGTATGATGGGACATGGTACCGT
  TGTATGAGCAGCACCCCAGCCGGCAGCATCGAGGCGCAAGCCCGTGTCCAAGTGCTGGAT
  GGGAGCAGCCTCCCAGAGTGGGTGACAGACAACGCTGGGACCCTGCATTTTGCCCGGGTG
  ACTCGAGATGACGCTGGCAACTACACTTGCATTGCCTCCAACGGGCCGCAGGGCCAGATT
  CGTGCCCATGTCCAGCTCACTGTGGCAGTTTTTATCACCTTCAAAGTGGAACCAGAGCGT
  ACGACTGTGTACCAGGGCCACACAGCCCTACTGCAGTGCGAGGCCCAGGGAGACCCCAAG
  CCGCTGATTCAGTGGAAAGGCAAGGACCGCATCCTGGACCCCACCAAGCTGGGACCCAGG
  ATGCACATCTTCCAGAATGGCTCCCTGGTGATCCATGACGTGGCCCCTGAGGACTCAGGC
  CGCTACACCTGCATTGCAGGCAACAGCTGCAACATCAAGCACACGGAGGCCCCCCTCTAT
  GTCGTGGACAAGCCTGTGCCGGAGGAGTCGGAGGGCCCTGGCAGCCCTCCCCCCTACAAG
  ATGATCCAGACCATTGGGTTGTCGGTGGGTGCCGCTGTGGCCTACATCATTGCCGTGCTG
  GGCCTCATGTTCTACTGCAAGAAGCGCTGCAAAGCCAAGCGGCTGCAGAAGCAGCCCGAG
  GGCGAGGAGCCAGAGATGGAATGCCTCAACGGTGGGCCTTTGCAGAACGGGCAGCCCTCA
  GCAGAGATCCAAGAAGAAGTGGCCTTGACCAGCTTGGGCTCCGGCCCCGCGGCCACCAAC
  AAACGCCACAGCACAAGTGATAAGATGCACTTCCCACGGTCTAGCCTGCAGCCCATCACC
  ACGCTGGGGAAGAGTGAGTTTGGGGAGGTGTTCCTGGCAAAGGCTCAGGGCTTGGAGGAG
  GGAGTGGCAGAGACCCTGGTACTTGTGAAGAGCCTGCAGAGCAAGGATGAGCAGCAGCAG
  CTGGACTTCCGGAGGGAGTTGGAGATGTTTGGGAAGCTGAACCACGCCAACGTGGTGCGG
  CTCCTGGGGCTGTGCCGGGAGGCTGAGCCCCACTACATGGTGCTGGAATATGTGGATCTG
  GGAGACCTCAAGCAGTTCCTGAGGATTTCCAAGAGCAAGGATGAAAAATTGAAGTCACAG
  CCCCTCAGCACCAAGCAGAAGGTGGCCCTATGCACCCAGGTAGCCCTGGGCATGGAGCAC
  CTGTCCAACAACCGCTTTGTGCATAAGGACTTGGCTGCGCGTAACTGCCTGGTCAGTGCC
  CAGAGACAAGTGAAGGTGTCTGCCCTGGGCCTCAGCAAGGATGTGTACAACAGTGAGTAC
  TACCACTTCCGCCAGGCCTGGGTGCCGCTGCGCTGGATGTCCCCCGAGGCCATCCTGGAG
  GGTGACTTCTCTACCAAGTCTGATGTCTGGGCCTTCGGTGTGCTGATGTGGGAAGTGTTT
  ACACATGGAGAGATGCCCCATGGTGGGCAGGCAGATGATGAAGTACTGGCAGATTTGCAG
  GCTGGGAAGGCTAGACTTCCTCAGCCCGAGGGCTGCCCTTCCAAACTCTATCGGCTGATG
  CAGCGCTGCTGGGCCCTCAGCCCCAAGGACCGGCCCTCCTTCAGTGAGATTGCCAGCGCC
  CTGGGAGACAGCACCGTGGACAGCAAGCCGTGA
  
  Clone variation with respect to NM_152880.2
  1731 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152880 unedited
  TTATTACACCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA
  AGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC
  GCGAATTCGGCACGAGGCCTCAGCTCCTTTTCCTGAGCCCGCCGCGATGGGAGCTGCGCG
  GGGATCCCCGGCCAGACCCCGCCGGTTGCCTCTGCTCAGCGTCCTGCTGCTGCCGCTGCT
  GGGCGGTACCCAGACAGCCATTGTCTTCATCAAGCAGCCGTCCTCCCAGGATGCACTGCA
  GGGGCGCCGGGCGCTGCTTCGCTGTGAGGTTGAGGCTCCGGGCCCGGTACATGTGTACTG
  GCTGCTCGATGGGGCCCCTGTCCAGGACACGGAGCGGCGTTTCGCCCAGGGCAGCAGCCT
  GAGCTTTGCAGCTGTGGACCGGCTGCAGGACTCTGGCACCTTCCAGTGTGTGGCTCGGGA
  TGATGTCACTGGAGAAGAAGCCCGCAGTGCCAACGCCTCCTTCAACATCAAATGGATTGA
  GGCAGGTCCTGTGGTCCTGAAGCATCCAGCCTCGGAAGCTGAGATCCAGCCACAGACCCA
  GGTCACACTTCGTTGCCACATTGATGGGCACCCTCGGCCCACCTACCAATGGTTCCGAGA
  TGGGACCCCCCTTTCTGATGGTCAGAGCAACCACACAGTCAGCAGCAAGGAGCGGGACCT
  GACGCTCCGGCCAGCTGGTCCTGAGCATAGTGGGCTGTATTCCTGCTGCGCCCACAGTGC
  TTTTGGCCCAGCTTGCAGCAGCCAGAACTTCAACTTGAGCATTGCTGATGAAAGCCTTGC
  CCAGGTGGTGCCTGGCCCCCCACGACGGGGTATTATCGAGGTATGAGGAGGCCTGTTCCT
  TTGACAGTTTCAGCCAGCCACCCCGGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_152880 unedited
  NGGGAAATCATGNACCGCGCCGCATTCTANGATCGATTTTTTTTTTTTTTTTTTGTAAAA
  AAGCTTATTTATTGAGNACAGCGAGTGTAAAAACAAAAACAAAAACAAAAAAACAAAACA
  AACAAAAAACGACAACAATAAAAGTGTGGGGTAAGGATGGCCCACCCTCCTCATCTCCAG
  GGCCCACCCCTACCTCCCATGCCCACCCACCCCACGCTGCAATTACATATAAAAGCCGCC
  CGTGGTGCATACAAAAAGGGCGGTTTATATAGTGTCAAAAGCTCCTGAAAACTCCTTCAT
  CTGCCAGGCACTTAGGATGAGGAAAGGAGAGAAGGGTGGGGAAGACGTGGGTCTGGGTCA
  GTGTGCACAAGCCCAGAGTGGGGAGGACTCACTTGCTGTGTCCTCTATAATCCTGGTCTA
  GACCCCCAAGTGGGACCAGTGGGCCAAGAGACTCATTAACCCTGTGTGCCCAGAACTTGA
  GAATATTAAGGAACTCCCCACAAACCCAGCTCATCCCAAGCCTAGCCCTCCCCAGGCAAA
  GTTGGCAGATGAGTGGCAGAGTTGGACCCGGTCAAGGGGAGAAGTTGAAGGCCAGAAATT
  GGGGTTACCTGTGGCACCCACACTGTCCCTGATAGAAGAAGAGGTGGCAGGGAAAACTGC
  CCAGCTCAAAGCCCTAGTCGCCCAGTTTGGGTCCAAGTCAGCCTTCCAAAAGATGAGGGG
  TGAGAAAAGGAGAAAAGCCACGCCCTGCTCAGACCTCAGCAATGCCTGTTGCACTAGGGC
  AGGGCCTCAGGCCCCACGAGGACCAGGATCCTGCCCATAAGCTGGAAGCTTTCTCTAGAA
  AGTCCCCCCTGGCCAGGCCATCCGAGCGGCTCCCTCCTAAAGGTTTGGGTCCCAGGGGCT
  GTTTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_152880
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152880.2, NP_690619.1
• RefSeq Size: 4153 bp
• RefSeq ORF: 3093 bp
• Locus ID: 5754
• Cytogenetics: 6p21.1
• Domains: pkinase, TyrKc, S_TKc, ig, IGc2, IG
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Gene Summary: 'This gene encodes a member of the receptor protein tyrosine kinase family of proteins that transduce extracellular signals across the cell membrane. The encoded protein lacks detectable catalytic tyrosine kinase activity, is involved in the Wnt signaling pathway and plays a role in multiple cellular processes including polarity and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]'
  Transcript Variant: This variant (PTK7-2) lacks an exon in the coding region, but maintains the reading frame, compared to variant PTK7-1. The encoded isoform (b) is shorter than isoform a.