IL1RA (IL1RN) (NM_173843) Human Untagged Clone

CAT#: SC101242

IL1RN (untagged)-Human interleukin 1 receptor antagonist (IL1RN), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IL1RN
• Synonyms: DIRA; ICIL-1RA; IL-1ra; IL-1ra3; IL-1RN; IL1F3; IL1RA; IRAP; MVCD4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC101242 sequence for NM_173843 edited (data generated by NextGen Sequencing)
  ATGCAAGCCTTCAGAATCTGGGATGTTAACCAGAAGACCTTCTATCTGAGGAACAACCAA
  CTAGTTGCCGGATACTTGCAAGGACCAAATGTCAATTTAGAAGAAAAGATAGATGTGGTA
  CCCATTGAGCCTCATGCTCTGTTCTTGGGAATCCATGGAGGGAAGATGTGCCTGTCCTGT
  GTCAAGTCTGGTGATGAGACCAGACTCCAGCTGGAGGCAGTTAACATCACTGACCTGAGC
  GAGAACAGAAAGCAGGACAAGCGCTTCGCCTTCATCCGCTCAGACAGTGGCCCCACCACC
  AGTTTTGAGTCTGCCGCCTGCCCCGGTTGGTTCCTCTGCACAGCGATGGAAGCTGACCAG
  CCCGTCAGCCTCACCAATATGCCTGACGAAGGCGTCATGGTCACCAAATTCTACTTCCAG
  GAGGACGAGTAG
  
  Clone variation with respect to NM_173843.2
  69 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_173843 unedited
  NGTTAGTTTTTGTNNTNNTTTTTTTTAGGGTGGNNGNGGNTTTGGTNGNGGGGGTGTTGT
  TTTGTGTTGTTTGGGGGTGGCGTGGGTTTAGAGACGATCTGCCGACCCTCTGGGGAGAAA
  ATCCAGCAAGATGCAAGCCTTCAGAATCTGGGATGTTAACCAGAAGACCTTCTATCTGAG
  GAACAACCAACTAGTTGCCGGATACTTGCAAGGACCAAATGTCAATTTAGAAGAAAAGAT
  AGATGTGGTACCCATTGAGCCTCATGCTCTGTTCTTGGGAATCCATGGAGGGAAGATGTG
  CCTGTCCTGTGTCAAGTCTGGTGATGAGACCAGACTCCAGCTGGAGGCAGTTAACATCAC
  TGACCTGAGCGAGAACAGAAAGCAGGACAAGCGCTTCGCCTTCATCCGCTCAGACAGTGG
  CCCCACCACCAGTTTTGAGTCTGCCGCCTGCCCCGGTTGGTTCCTCTGCACAGCGCTGGG
  AGCTGACCAGCCCGTCGGCCTCACCAATATGCCTGACGAAAGCGTCTGGGTCACCAAAAT
  CTAGCTCCAGTAGGACCAGGTGTGCTGCCCAGGCCCGGGCCGATCCCCTTCCTCGATGGG
  CAGGGACCGCAGGCACTGCACAGTCCCCCTGCCCCCGGGGCTCCCCGCCTTTGGCGCCCC
  CTGGCGCACACACCCTTGAGGCGCCGGACCCCCACAAAGCGTCCGCGGCACCGGGGAAGC
  GGGGACGTGCGCCTCCCCGGCGACGGACGAGGCCGCCCAGCTCGCCCCCCCACAGGGACG
  TTAAGAGGGGGCACACTCGGAGCAACGAGAGCCACGGGCACAAGGCCTTTCCCCGTCGCC
  TCGGATATGAGTAGAACACGACCCGAGACACCATGGAACAAACTGGTACGACTCTTCTGA
  AACAGGGTTCATCCTCACCGATATTACAACCAACACCGGCGCCAGCAAACACCCG
  
• Restriction Sites: Please inquire
• ACCN: NM_173843
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173843.1, NP_776215.1
• RefSeq Size: 1973 bp
• RefSeq ORF: 432 bp
• Locus ID: 3557
• Cytogenetics: 2q14.1
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses, particularly in the acute phase of infection and inflammation. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Aug 2020]'
  Transcript Variant: This variant (4) contains a distinct internal exon compared to variant 2. This results in translation from a downstream start codon, and an isoform (4) that has a shorter N-terminus, as compared to the longest isoform (2). Variants 4 and 5 both encode the same isoform (4).