PSMD5 (AK001065) Human Untagged Clone

CAT#: SC101418

(untagged)-Human cDNA FLJ10203 fis, clone HEMBA1004930, moderately similar to 26S PROTEASOME SUBUNIT S5B

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMD5
• Synonyms: S5B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK001065, the custom clone sequence may differ by one or more nucleotides
  
  
  AAAAACTGATGAAGGAAATTGAAGAAGACACAAATAAATGGAAAGGTATCCTATGTTCATGGATTGGAAG
  GATTAATATTATTAAAATGTTCATGCTGCCCAAAGCACTATATAGATTCAGTGCATTCCCTCCAAAATTT
  TTAAATTTAATGACATTTTTCACGAAAGCAGAAAAAATAATTTTTAAATATACATATTTCAGAATACATT
  GTTCACAATAAAGATATATGATTTTTTGTCAGTTAAAAGTTAATTTTTTTAAAAAGATACCTATAGCCTC
  TTATTGAGGTGGAATCTGTTGCTCTTGGAGCTACGTACTGTTGACTTTGGTTTTCTGGAAACATCAGTGC
  TATTTAGAACATTAGGATATTTTTCTTTTTGAATATCAACAAAAACATAAATAAAAATACTAGTAATGAC
  ATTTACAGTATGTTTCACAGATTAATCACATCTCTGAGTGGGAGTCTTTATCTGTTGTAGGATCTACTCT
  TGCCATTCTATTCTGGGTTTTGGTTGATTAGTCTTTCTTGAACACTTCCAGAAATTGGAGATGTATGCCT
  GCCTTAGGTAGCCTAGCTTATTTTATTAATGAACATATCCATTTAAAAATTTCTTCCTGGTAATGAGTCT
  AAACTTGCCTTCCTGTAATTTGTATCCACTGGTCCTTTTTCTTTGTTCTCCCTCTTGCATATGCCAGTAC
  TTCAATAGCTGGTCCCAGCTCCATTGACGCCCTCTCTCAGGATCACACATCACAGGTCCTTCAGAATCTC
  CTAATGGTCTCTCTGATCTCCAGGCAACACACTACTCTAACCTTTCTATGCTTTTCTGGCCTGCAGTTCA
  TTTCTGTTCCTGTGAAGTCATAGAGCCCGCAATTCATGCTGTCTTGTTCCCCTATTAAAATATATTTTTG
  TAAATCTCTTTAGGATTCGTGAAGTTTTTTGGAAACCTGGCTGTCATGGATAGTCCTCAACAGATCTGTG
  AGCGTTATCCTATCTTTGTGGAAAAAGTCTTTGAAATGATAGAAAGTCAGGACCCCACTATGATTGGTGT
  AGCTGTAGACACAGTTGGAATCTTGGGATCCAATGTTGAAGGAAAACAGGTTTTACAGAAAACAGGTTGG
  TATGACTTGGCCCTGTTCAGGGATATCTTAATCTTTGACAGGGGTTCAATATGTAAGTTCATTTGCTGGT
  GGGTTAACTACATTGTAGAAACTATTTTATGGGTGGCAGTCTTGTTAACCATGACCTTGTACTCATGTCT
  GAAAATCTAATAATTATTACGGAAAACCTTTTTTTTTTTTTTTTGACTTTTCACTGGTTTAGGTTTTCAT
  TGGTTTAGGTTTCTACCAATCGATTTCAACCAAACATTGTTTCCTGTTTCCCCTCTATGATGTCTTCTTT
  GACTAGATTTCATGGTACAAAGACCACGGGCTAGAGTCAGAAAGGTCTGGATTCATGTCCTTGGGTTTTC
  TATTCTTTTTTTTTTTTTTTTTTTAAGAGATTGAGAGTGTCTCTCTGTTACCCAGGCTGGCCCCGAACTC
  CCGGGCTCAAGCAATCCTCCTGCCTCATCCTCTTGATTGGCTGGGTCTACAGGTCCACACCATTGTATCC
  AGCCCTGACTTTGCTTTTTGAACTTCATTTACTCACTTCACTTTTCTAGGCCTCATTTTCTTCATTTATA
  AAAATGGCATAACAACTGCTTCTCAGGGTTGTTGTGAATATTTATGAAATTTTGAGAAAGGAAAATAAAA
  GTTTGTTATAAGATTTCATGTTAAGAGATACTTTTGGCATGTTAAGAGATACAATGGTTCATGCCTGTAA
  TCCAGCACTTTGGGAGGCTAAGGTGGGTGGGTTGCCTGAGCTCAGGAGTTCAAGACCAGGCTGGGCAACA
  TGGCAAAATCCTATCTCTACTCAAGAATACAAAAATTAACCAGGCATGGTACTGCACAACTGTAGTCCCA
  GCTACTTGAGAAGCTGAGGAAGGAGAATCTCTTGAACCTGGCAGGTGGAGGTTGCAGTGAGCTGAGATCA
  TGCCACTACACTCCAGCCTGGGTGACAGAGTGAAATTCTATCTCAAAAAAAAAAAAAAAAAAGAGGCCAG
  GTATGGTGGCTCATGCCTGTAATCCCAGCATTTTGGGAGGCCAAAGCAGGTGGATCACTTGAGGTCAGGA
  GCTCGAGACCAGCCTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAGTACAAAAATCAGCCAAGCGTG
  GTGGTGCATGCCTGCAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGCATGAACACAGGAGGTAA
  AGGTTGCAGTGAGCCGAGATCACACCACTGCACTCCAGCCTGGGCGACAGTGCGAGACTCTGT
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK001065 unedited
  GTCAGAAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTAGCCTAGC
  TTATTTTATTAATGAACATATCCATTTAAAAATTTCTTCCTGGTAATGAGTCTAAACTTG
  CCTTCCTGTAATTTGTATCCACTGGTCCTTTTTCTTTGTTCTCCCTCTTGCATATGCCAG
  TACTTCAATAGCTGGTCCCAGCTCCATTGACGCCCTCTCTCAGGATCACACATCACAGGT
  CCTTCAGAATCTCCTAATGGTCTCTCTGATCTCCAGGCAACACACTACTCTAACCTTTCT
  ATGCTTTTCTGGCCTGCAGTTCATTTCTGTTCCTGTGAAGTCATAGAGCCCGCAATTCAT
  GCTGTCTTGTTCCCCTATTAAAATATATTTTTGTAAATCTCTTTAGGATTCGTGAAGTTT
  TTTGGAAACCTGGCTGTCATGGATAGTCCTCAACAGATCTGTGAGCGTTATCCTATCTTT
  GTGGAAAAAGTCTTTGAAATGATAGAAAGTCAGGACCCCACTATGATTGGTGTAGCTGTA
  GACACAGTTGGAATCTTGGGATCCAATGTTGAAGGAAAACAGGTTTTACAGAAAACAGGT
  TGGTATGACTTGGCCCTGTTCAGGGATATCTTCATCTTTGACAGGGGTTCAATATGTAAG
  TTCATTTGCTGGTGGGTTAACTACATTGTAGAAACTATTTTATGGGTGGCAGTCTTGTTA
  ACCATGACCTTGTACTCATGTCTGAAAATCTAATAATTATTACGGAAAAACCTTTTTTTT
  TTTTTTTTGACTTTTCACTGGTTTAGGTTNTCATTGGTTTANGTTTCTACCAATCGATTT
  CAACCAAACATTGTTTCCTGTTTCCCCTCTATGATGTCTTCTTTNGACTAGATTCATGGN
  ACAAAGACACNGNCTANAGTCAGAAAAGTCTGGATTCATGTCCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK001065 unedited
  NNAAAATACTGTGNACCGCGGCCCGCTATCTANATCGAGTTTTTTTTTTTTTTTTTTACA
  GAGTCTCGCACTGTCGCCCAGGCTGAAGTGCAGTGGTGTGATCTCGGCTCACTGCAACCT
  TTACCTCCTGTGTTCATGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTGCAGG
  CATGCACCACCACGCTTGGCTGATTTTTGTACTTTTTTTTTTTACAGGGTTTCACCATGT
  TGGCCAGGCTGGTCTCGAGCTCCTGACCTCAAGTGATCCACCTGCTTTGGCCTCCCAAAA
  TGCTGGGATTACAGGCATGAGCCACCATACCTGGCCTCTTTTTTTTTTTTTTTTTTTTGA
  GAAAAAATTTCACTCTGTCACCCAAGCTGGAGTGTAATGGCATGATCTCAGCTCACTGCA
  ACCTCCACCTGCCAGGTTCAAGAAATTCTCCCTCCTCAGCCTCTCAAGTAGCTGGGACTA
  CAGTTGTGCAATACCATGCCTGGTTAATTTTTGTATTCTTGAGTAGAGATAGGATTTTGC
  CATGTTGCCCAGCCTGGTCTTGAACTCCTGAGCTCAGGCAACCCACCCACCTTAGCCTCC
  CAAAATGCTGGATTACAGGCATGAACCATTGTATCTCTTAAAAGCCCAAAGTATCTCTTA
  AACATGAAAACTTAAAAAAAATTTTTTTTTTCTTTTCCAAATTTTATAATATTTAACAAA
  AACCCTGAAAAACCTTTGTATGCCATTTTATAAATTAACAAAAGTAGGCCTCAAAAATTA
  ATTGGGTTAATGAATTTCAAAACCAAATTCCGGCTGGAATCATGGGGGGGACCCTTTAAC
  CCCCCCCCCTAACAAGTTAAGCCCGGAGGATTTGTTTAACCCGGGAATTA
  
• Restriction Sites: NotI-NotI
• ACCN: AK001065
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK001065.1
• RefSeq Size: 2373 bp
• RefSeq ORF: 2373 bp
• Locus ID: 5711
• Cytogenetics: 9q33.2
• Gene Summary: 'The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a non-ATPase subunit of the 19S regulator base that functions as a chaperone protein during 26S proteasome assembly. [provided by RefSeq, Jul 2012]'