PPP2R5C (NM_178586) Human Untagged Clone

CAT#: SC101725

PPP2R5C (untagged)-Human protein phosphatase 2, regulatory subunit B', gamma (PPP2R5C), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP2R5C
• Synonyms: B56G; B56gamma; PR61G
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_178586 edited
  TTTTTTTTTTTTTTTAAACTAAAATGGAGGCTGGTTTCTTGCCTTAAGGAGCCCATTGCC
  TTTCCCGCTGAAGTCTAGATGTTGACATGTAATAAAGCGGGCAGCAGGATGGTGGTGGAT
  GCGGCCAACTCCAATGGGCCTTTCCAGCCCGTGGTCCTTCTCCATATTCGAGATGTTCCT
  CCTGCTGATCAAGAGAAGCTTTTTATCCAGAAGTTACGTCAGTGTTGCGTCCTCTTTGAC
  TTTGTTTCTGATCCACTAAGTGACCTAAAGTGGAAGGAAGTAAAACGAGCTGCTTTAAGT
  GAAATGGTAGAATATATCACCCATAATCGGAATGTGATCACAGAGCCTATTTACCCAGAA
  GTAGTCCATATGTTTGCAGTTAACATGTTTCGAACATTACCACCTTCCTCCAATCCTACG
  GGAGCGGAATTTGACCCGGAGGAAGATGAACCAACGTTAGAAGCAGCCTGGCCTCATCTA
  CAGCTTGTTTATGAATTTTTCTTAAGATTTTTAGAGTCTCCAGATTTCCAACCTAATATA
  GCGAAGAAATATATTGATCAGAAGTTTGTATTGCAGCTTTTAGAGCTCTTTGACAGTGAA
  GATCCTCGGGAGAGAGATTTTCTTAAAACCACCCTTCACAGAATCTATGGGAAATTCCTA
  GGCTTGAGAGCTTACATCAGAAAACAGATAAATAATATATTTTATAGGTTTATTTATGAA
  ACAGAGCATCATAATGGCATAGCAGAGTTACTGGAAATATTGGGAAGTATAATTAATGGA
  TTTGCCTTACCACTAAAAGAAGAGCACAAGATTTTCTTATTGAAGGTGTTACTACCTTTG
  CACAAAGTGAAATCTCTGAGTGTCTACCATCCCCAGCTGGCATACTGTGTAGTGCAGTTT
  TTAGAAAAGGACAGCACCCTCACGGAACCAGTGGTGATGGCACTTCTCAAATACTGGCCA
  AAGACTCACAGTCCAAAAGAAGTAATGTTCTTAAACGAATTAGAAGAGATTTTAGATGTC
  ATTGAACCATCAGAATTTGTGAAGATCATGGAACCCCTCTTCCGGCAGTTGGCCAAATGT
  GTCTCCAGCCCACACTTCCAGGTGGCAGAGCGAGCTCTCTATTACTGGAATAATGAATAC
  ATCATGAGTTTAATCAGTGACAACGCAGCGAAGATTCTGCCCATCATGTTTCCTTCCTTG
  TACCGCAACTCAAAGACCCATTGGAACAAGACAATACATGGCTTGATATACAACGCCCTG
  AAGCTCTTCATGGAGATGAACCAAAAGCTATTTGATGACTGTACACAACAGTTCAAAGCA
  GAGAAACTAAAAGAGAAGCTAAAAATGAAAGAACGGGAAGAAGCATGGGTTAAAATAGAA
  AATCTAGCCAAAGCCAATCCCCAGGCACAGAAAGATCCGAAGAAGGACCGTCCTCTTGCA
  CGCCGCAAGTCCGAGCTGCCTCAGGACCCCCACACCAAGAAAGCCTTGGAAGCTCACTGC
  AGGGCCGATGAGCTGGCCTCCCAGGACGGCCGCTAGCCTCCGGGGCGCCGCGTCGGGGCC
  GGGCCCGCCAGTTCTTTTCCGGATTCTGTAGAAAATACATACTTCCTGTGCCATACCAAT
  CAGTTACACTCAAAGCTTTCTTGGACCCCGTTCCGTAGGCAATAACGTGCGTCCGCCTCA
  GCGCGAGATTAGGAGTTCAAACAATGGTGACTTCCCAGAGCCCGCTGGCAGAGCCGCGGG
  TTGACGACGGTGTCCTCGCAGTGTCGCCGCCACCCCAGCGTAGTCCAAGTCAGACTATTT
  CACAAGTCAGAGCGATAGGAAAGCACCCTGCCCTTCATCTTCATGTTCTCCCAAAT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_178586 unedited
  TTTGCCNAGTCAGATTTGTAAACTATCATATAGGCGGCGCGCGAATTCGCACCAGGAGTA
  ACTAGTCTCGAGTTTTTTTTTTTTTTTTTAAACTAAAATGGAGGCTGGTTTCTTGCCTTA
  AGGAGCCCATTGCCTTTCCCGCTGAAGTCTAGATGTTGACATGTAATAAAGCGGGCAGCA
  GGATGGTGGTGGATGCGGCCAACTCCAATGGGCCTTTCCAGCCCGTGGTCCTTCTCCATA
  TTCGAGATGTTCCTCCTGCTGATCAAGAGAAGCTTTTTATCCAGAAGTTACGTCAGTGTT
  GCGTCCTCTTTGACTTTGTTTCTGATCCACTAAGTGACCTAAAGTGGAAGGAAGTAAAAC
  GAGCTGCTTTAAGTGAAATGGTAGAATATATCACCCATAATCGGAATGTGATCACAGAGC
  CTATTTACCCAGAAGTAGTCCATATGTTTGCAGTTAACATGTTTCGAACATTACCACCTT
  CCTCCAATCCTACGGGAGCGGAATTTGACCCGGAGGAAGATGAACCAACGTTAGAAGCAG
  CCTGGCCTCATCTACAGCTTGTTTATGAATTTTTCTTAAGATTTTTAGAGTCTCCAGATT
  TCCAACCTAATATAGCGAAGAAATATATTGATCAGAAGTTTGTATTGCAGCTTTTAGAGC
  TCTTTGACAGTGAAGATCCTCGGGAGAGAGATTTTCTTAAAACCACCCTTCACAGAATCT
  ATGGGAAATTCCTAGGCTTGAGAGCTTACATCTGAAAACAGATCAATAATATATTTTATA
  GGTTTATTTATGAAACAGAGCATCATAATGGCATAGCAGAGTTACTGGGAATATTGGGAA
  GTATAATT
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_178586 unedited
  NGGTTTATGGCTTCTGTGTAGTGCAGTTTTTANAAAAGGCANCACCCTCACGAACCAGTG
  GTGATGGCACTTCTCAATACTGGCCAAAGACTCACAGTCCAAAAGAAGTAATGTTCTTAA
  ACGAATTAGAAGAGATTTTAGATGTCATTGAACCATCAGAATTTGTGAAGATCATGGAAC
  CCCTCTTCCGGCAGTTGGCCAAATGTGTCTCCAGCCCACACTTCCAGGTGGCAGAGCGAG
  CTCTCTATTACTGGAATAATGAATACATCATGAGTTTAATCAGTGACAACGCAGCGAAGA
  TTCTGCCCATCATGTTTCCTTCCTTGTACCGCAACTCAAAGACCCATTGGAACAAGACAA
  TACATGGCTTGATATACAACGCCCTGAAGCTCTTCATGGAGATGAACCAAAAGCTATTTG
  ATGACTGTACACAACAGTTCAAAGCAGAGAAACTAAAAGAGAAGCTAAAAATGAAAGAAC
  GGGAAGAAGCATGGGTTAAAATAGAAAATCTAGCCAAAGCCAATCCCCAGGCACAGAAAG
  ATCCGAAGAAGGACCGTCCTCTTGCACGCCGCAAGTCCGAGCTGCCTCAGGACCCCCACA
  CCAAGAAAGCCTTGGAAGCTCACTGCAGGGCCGATGAGCTGGCCTCCCAGGACGGCCGCT
  AGCCTCCGGGGCGCCGCGTCGGGGCCGGGCCCGCCAGTTCTTTTCCGGATTCTGTAGAAA
  ATACATACTTCCTGTGCCATACCAATCAGTTACACTCAAAGCTTTCTTGGACCCCGTTCC
  GTANGCAATAACGTGCGTCCGCCTCAGCGCGAGATTAGGAGTTCAAANCATGGTGACTTC
  CCAGAGCCCGCTGGCAGAGCCGCGGGTTGACGACNGTGTCCTCGCAGTGTCGCCGCACCC
  CAGCGTAGTCCAGTCAGACTATTTC
  
• Restriction Sites: Please inquire
• ACCN: NM_178586
• Insert Size: 1550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178586.1, NP_848701.1
• RefSeq Size: 4050 bp
• RefSeq ORF: 1458 bp
• Locus ID: 5527
• Cytogenetics: 14q32.31
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Oocyte meiosis, Wnt signaling pathway
• Gene Summary: 'The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a gamma isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (b), also known as gamma 2.