PSMC5 (AK098484) Human Untagged Clone

CAT#: SC101816

(untagged)-Human cDNA FLJ25618 fis, clone STM02139, highly similar to 26S PROTEASE REGULATORY SUBUNIT 8

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMC5
• Synonyms: S8|SUG-1|SUG1|TBP10|TRIP1|p45|p45; SUG
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK098484, the custom clone sequence may differ by one or more nucleotides
  
  
  GGATGCCGGCGGTCTCTGCTGAAGAGAGAAGATGGCGCTTGACGGACCAGAGCAGGTATGGCGGGTGCAG
  TGGCGGCCCGGCAGGTTACGGGGCTGGGTGCGGAGCGAGCGTGATCTGAGTGGAGAGCGGGCCGGGGCAG
  GAGCGTCGGGTGGGTCCATGGATTTTCAATTTCACCCTAACCCCCTGCGTTGTTCAAGTGTCAACTGTAA
  TATAAATTGGATGGGGGAAAGCCACAAAAGACAATTCTGAATTCCAAGTGCATTTGCAGGTATTATGTTT
  AGTTTACCATTTTTGAATGCTTTTTCATGTGTTTTTATTCGTTCACCTTAGTAGTCTTGAAGCAGGGTAG
  CTGATGCTATACCCATGTGCCGTTTGGTAACAGATCTAGATTTTTAAAAATAGATTGTCTTACCTTTACT
  TTGCTTTTGATTGACAGCGGCTGGGCTAGGACTCAGGTTTCTCACCTAACAATCTTTCCACCAGGTGTTG
  TTGCCTTCCTGCAAGAAATTTGGTGTTACATGAGCCAGCTGTAACTTTTCTCCATTAGATTCTGTTACTC
  CTGGACCAGTATTGGACAAGCAGCCTTCTTTGATGGCTGCTGTAAGGTCCAGAGGGACTCTTGAAGGACA
  GGGCAGGGTCATTAGGATCATACTGGAGAATCTGAAACAACATGCAGGTGCCCTGTGCCCTTCCCTCAGC
  TCATTCAGACCTGTGAGGTCTTGGCCAAGATCCTACCTCCTCCTGTCTCCTGCCCTTGAATGATGGAATT
  TGTCTCTTGTCTGCCACAGCTGATTGTGAATGATAAGAGCCAAAACCTCCGGAGGCTGCAGGCACAGAGG
  AACGAACTAAATGCTAAAGGTGAGTGGAGAAAGATGGGAAGCCGCATGTGGGCAGTTTGTCTGAGGTCTG
  TCCTTATCTCCCTGCACTGTGTCTTCACAGCAGTGCTTACTGTTTTCTCCCTGTCATCATCTAGTAGTTT
  CACATGCATCTCTTTTTCCTGAGCCCTATTGTAGCCTCCTTAAATACATGAATTTTGACCTTTGGCCTTC
  CTTTAATAAGAGTGATGCTTAGCTCATGCACGTAGAATTGGGTCCTGGAGACTCCAAAGGAGTAGCTAGG
  TGACCACTGTGTCTGTTTGCCATCTAGTTCGCCTATTGCGGGAGGAGCTACAGCTGCTGCAGGAGCAGGG
  CTCCTATGTGGGGGAAGTAGTCCGGGCCATGGATAAGAAGAAAGTGTTGGTCAAGGTAAAAGCAGCATGA
  CCCCAGGGACCAGCTCGGTCTCCACTGCATTCCCACCCCTTTGTGTGTAGCCTCGGGAGACAGGGTTCTG
  TGTTCTGTCAAGGTATTGTGGGATTCTCATAGGTCTTCCTGGGTAGGATTAGTGATTTGGTGGCTGTCAA
  GGAAGGTCATGAGCCCTTGTTTCTTTAGGTACATCCTGAAGGTAAATTTGTTGTAGACGTGGACAAAAAC
  ATTGACATCAATGATGTGAGTGTAGCAGGTGAGGTGGTGGTGGTGGTGGGGTCAGCTCTTACCGTACCAC
  TTCTGAAACTCGCCCCCTTCACCCAGGTGACACCCAATTGCCGGGTGGCTCTAAGGAATGACAGCTACAC
  TCTGCACAAGATCCTGCCCAACAAGGTAGACCCATTAGTGTCACTGATGATGGTGGAGAAAGTACCAGAT
  TCAACTTATGAGATGATTGGTGGACTGGACAAACAGATCAAGGAGATCAAAGAAGTGATCGAGCTGCCTG
  TTAAGCATCCTGAGCTCTTCGAAGCACTGGGCATTGCTCAGCCCAAGGGAGTGCTGCTGTATGGACCTCC
  AGGCACTGGGAAGACACTGTTGGCCCGGGCTGTGGCTCATCATACGGACTGTACCTTTATTCGTGTCTCT
  GGCTCTGAACTGGTACAGAAATTCATAGGGGAAGGTAACCATGGCTAGCAATGTGAGAAGCAAGAGGTAG
  GGGTAGGGGGTTAGAGAGCTAATAAGCTAATAAGCTCCCTAACACCAGCTCGGCCTCCACACAGGGGCAA
  GAATGGTGAGGGAGCTGTTTGTCATGGCACGGGAACATGCTCCATCTATCATCTTCATGGACGAAATCGA
  CTCCATCTCTTTGAGGGTCTTAGTGTTCACAGTTTGTTACACATCTTCGAGTCAAATCTTAATAGTAAAG
  GTACCTCTAGCCAAAGGTCAACCCTTTTGGAAGAAGCCCGAATGAAAAGGATTCTTATAAAAGGGAAACA
  TAAGCTCAGGAGTTGAAATTTATCACCAAGAGGTTGGGTGAGGGTGAAGTGAGAATGTGAGGAAGGGCAT
  CATTGAGGGATCTGGACAATGCTAATTCCATAGAGACTGAATGAAAGAAATTGGGCAACCCAGAGCCCTA
  GCCTAAAGCGCTAAACCATAGAAAAAGGACCGTTTATGTTGTTAATCAAATGTTTGTGTTACCAGTAAGG
  CTTCCAGCAGTAAGGTATGGCTTCCAGTAGTAAGCCTTACAGACAACATAAACAGTAGTCTGTTAGTGTT
  ACGTTATACTGTTACGGTAGGCTGTTAGTAGTTTAACTTTGGACAGGTCCATGGATTTTCAATTTCACCC
  TAACCCCCTGCGTTGTTCAAGTGTCAACTGTAATATAAATTGGATGGGGGAAAGCCACAAAAGACAATTC
  TGAATTCCAAGTGCAGCGCACGATGCTGGAGTTGCTCAACCAGCTCGACGGCTTTGAGGCCACCAAGAAC
  ATCAAGGTTATCATGGCTACTAATAGGATTGATATCCTGGACTCGGCACTGCTTCGCCCAGGGCGCATTG
  ACAGAAAAATTGAATTCCCACCCCCCAATGAGGAGGCCCGGCTGGACATTTTGAAGATTCATTCTCGGAA
  GATGAACCTGACCCGGGGGATCAACCTGAGAAAAATTGCTGAGCTCATGCCAGGAGCATCAGGGGCTGAA
  GTGAAGGGCGTGTGCACAGAAGCTGGCATGTATGCCCTGCGAGAACGGCGAGTCCATGTCACTCAGGAGG
  ACTTTGAGATGGCAGTAGCCAAGGTCATGCAGAAGGACAGTGAGAAAAACATGTCCATCAAGAAATTATG
  GAAGTGAGTGGACAGCCTTTGTGTGTATCTCTCCAATAAAGCTCTGTGGGCCAAGTCC
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK098484 unedited
  ACACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCTGAAGAGAAAAAGGCG
  CTTGACGGACCAGAGCAGATGGAGCTGGAGGAGGGGAAGGCAGGCAGCGGACTCCGCCAA
  TATTATCTGTCCAAGATTGAAGAACTCCAGCTGATTGTGAATGATAAGAGCCAAAACCTC
  CGGAGGCTGCAGGCACAGAGGAACGAACTAAATGCTAAAGTTCGCCTATTGCGGGAGGAG
  CTACAGCTGCTGCAGGAGCAGGGCTCCTATGTGGGGGAAGTAGTCCGGGCCATGGATAAG
  AAGAAAGTGTTGGTCAAGGTACATCCTGAAGGTAAATTTGTTGTAGACGTGGACAAAAAC
  ATTGACATCAATGATGTGACACCCAATTGCCGGGTGGCTCTAAGGAATGACAGCTACACT
  CTGCACAAGATCCTGCCCAACAAGGTAGACCCATTAGTGTCACTGATGATGGTGGAGAAA
  GTACCAGATTCAACTTATGAGATGATTGGTGGACTGGACAAACAGATCAAGGAGATCAAA
  GAAGTGATCGAGCTGCCTGTTAAGCATCCTGAGCTCTTCGAAGCACTGGGCATTGCTCAG
  CCCAAGGGAGTGCTGCTGTATGGACCTCCAGGCACTGGGAAGACACTGTTGGCCCGGGCT
  GTGGCTCATCATACGGACTGTACCTTTATTCGTGTCTCTGGCTCTGNATTGGTACAGAAA
  TTCATAGGGGAAGGGGGCAGAATGGTGANGGGAGCTNGTTGTCATGGCACGGGAACATGC
  TCCATCTATCATCTTCATGGACGAAATCGACTCCCATCGCTTCTCGCGCTGGAGAGGGGG
  GTTCTGGAGGNGACAANTGAANTGCACGCACGATGCCTGNAGTGCTCACCANCTCGACGC
  TTTTGAGGCACCAGACATCAGGNTATCATGGCTCTAATAGGATNGANTCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK098484 unedited
  NGGTACTACTATGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTAC
  TTGGCCCACANAGCTTTATTGGAGAGNAACACACAAAGGCTGTCCACTCACTTCCATAAT
  TTCTTGATGGACATGTTTTTCTCACTGTCCTTCTGCATGACCTTGGCTACTGCCATCTCA
  AAGTCCTCCTGAGTGACATGGACTCGCCGTTCTCGCAGGGCATACATGCCAGCTTCTGTG
  CACACGCCCTTCACTTCAGCCCCTGATGCTCCTGGCATGAGCTCAGCAATTTTTCTCAGG
  TTGATCCCCCGGGTCAGGTTCATCTTCCGAGAATGAATCTTCAAAATGTCCAGCCGGGCC
  TCCTCATTGGGGGGTGGGAATTCAATTTTTCTGTCAATGCGCCCTGGGCGAAGCAGTGCC
  GAGTCCAGGATATCAATCCTATTAGTAGCCATGATAACCTTGATGTTCTTGGTGGCCTCA
  AAGCCGTCGAGCTGGTTGAGCAACTCCAGCATCGTGCGCTGCACTTCACTGTCCCCTCCA
  GAACCCCCCTCCAGCCGCGAGGAGCCGATGGAGTCGATTTCGTCCATGAAGATGATAGAT
  GGAGCATGTTCCCGTGCCATGACAAACAGCTCCCTCACCATTCTTGCCCCTTCCCCTATG
  AATTTCTGTACCAATTCAGAGCCAGAGACACGAATAAAGGTACAGTCCGTATGATGAGCC
  ACAGCCCGGGCCAACAGTGTCTTCCCAGTGCCTGGAGGTCCATACAGCAGCACTCCCTTG
  GGCTGAGCATGCCCAGTGCTTCGAAGAGCTCAGGATGCTTNACAGGCAGCTCGATCACTT
  CTTGATCTCCTTGATCTGGTTGTCCAGTCCACCATCATCTCATAAGTTGGATCTGGGACT
  TTTCTCCCCN
  
• Restriction Sites: NotI-NotI
• ACCN: AK098484
• Insert Size: 1250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK098484.1
• RefSeq Size: 3138 bp
• RefSeq ORF: 3138 bp
• Locus ID: 5705
• Cytogenetics: 17q23.3
• Domains: AAA
• Protein Families: Druggable Genome
• Protein Pathways: Proteasome
• Gene Summary: 'The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown to interact with the thyroid hormone receptor and retinoid X receptor-alpha. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]'