RPS24 (NM_033022) Human Untagged Clone

CAT#: SC101869

RPS24 (untagged)-Human ribosomal protein S24 (RPS24), transcript variant a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPS24
• Synonyms: DBA3; eS24; S24
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC101869 sequence for NM_033022 edited (data generated by NextGen Sequencing)
  ATGAACGACACCGTAACTATCCGCACTAGAAAGTTCATGACCAACCGACTACTTCAGAGG
  AAACAAATGGTCATTGATGTCCTTCACCCCGGGAAGGCGACAGTGCCTAAGACAGAAATT
  CGGGAAAAACTAGCCAAAATGTACAAGACCACACCGGATGTCATCTTTGTATTTGGATTC
  AGAACTCATTTTGGTGGTGGCAAGACAACTGGCTTTGGCATGATTTATGATTCCCTGGAT
  TATGCAAAGAAAAATGAACCCAAACATAGACTTGCAAGACATGGCCTGTATGAGAAGAAA
  AAGACCTCAAGAAAGCAACGAAAGGAACGCAAGAACAGAATGAAGAAAGTCAGGGGGACT
  GCAAAGGCCAATGTTGGTGCTGGCAAAAAGTGA
  
  Clone variation with respect to NM_033022.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033022 unedited
  GGAAGCGTTCATTATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGCCTC
  GTGCCGAATTCGGCACGAGGATAGATCGCCATCATGAACGACACCGTAACTATCCGCACT
  AGAAAGTTCATGACCAACCGACTACTTCAGAGGAAACAAATGGTCATTGATGTCCTTCAC
  CCCGGGAAGGCGACAGTGCCTAAGACAGAAATTCGGGAAAAACTAGCCAAAATGTACAAG
  ACCACACCGGATGTCATCTTTGTATTTGGATTCAGAACTCATTTTGGTGGTGGCAAGACA
  ACTGGCTTTGGCATGATTTATGATTCCCTGGATTATGCAAAGAAAAATGAACCCAAACAT
  AGACTTGCAAGACATGGCCTGTATGAGAAGAAAAAGACCTCAAGAAAGCAACGAAAGGAA
  CGCAAGAACAGAATGAAGAAAGTCAGGGGGACTGCAAAGGCCAATGTTGGTGCTGGCAAA
  AAGTGAGCTGGAGATTGGATCACAGCCGAAGGAGTAAAGGTGCTGCAATGATGTTAGCTG
  TGGCCACTGTGGATTTTTCGCAAGAACATTAATAAACTAAAAACTTCAAAAAAAAAAAAA
  AAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTG
  GCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCC
  CACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTA
  TAATATTATGGGTGGGGGGGGGTGGGGATATGGAACAAGGGGCAAATTTGGGAAGAACAC
  CTCGTAGGGCCTGGCGGGGTCTTATGGGGAACCCAACCTTGGAGTGCAGTTGGCACCACT
  CTTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033022 unedited
  GCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGAAGTTTTTAGTTTATTAAT
  GTTCTTGCGAAAAATCCACAGTGGCCACAGCTAACATCATTGCAGCACCTTTACTCCTTC
  GGCTGTGATCCAATCTCCAGCTCACTTTTTGCCAGCACCAACATTGGCCTTTGCAGTCCC
  CCTGACTTTCTTCATTCTGTTCTTGCGTTCCTTTCGTTGCTTTCTTGAGGTCTTTTTCTT
  CTCATACAGGCCATGTCTTGCAAGTCTATGTTTGGGTTCATTTTTCTTTGCATAATCCAG
  GGAATCATAAATCATGCCAAAGCCAGTTGTCTTGCCACCACCAAAATGAGTTCTGAATCC
  AAATACAAAGATGACATCCGGTGTGGTCTTGTACATTTTGGCTAGTTTTTCCCGAATTTC
  TGTCTTAGGCACTGTCGCCTTCCCGGGGTGAAGGACATCAATGACCATTTGTTTCCTCTG
  AAGTAGTCGGTTGGTCATGAACTTTCTAGTGCGGATAGTTACGGTGTCGTTCATGATGGC
  GATCTATCCTCGTGCCGAATTCGGCACGAGGCTCGTGCCGAATTCGCGGCCGCCCTATAG
  TGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTNCC
  ACCGTACACGCCTACCGNNCATTTGCGTCAACGGGGCGGGGTTTATACGACATTTTGGAA
  AGTCCCNGTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTGG
  NAAATCCCCGTGAGTCAAACGCTATCCACGCCCATGGTGTACTGCCAAACCGCATCACAT
  GGTATAGCGAGACTATACGTANATGTCTGCCAGTAGGAAGTCCGTAAGTCTGGACTGGCA
  TAAGCCAGCGGGCCTTTACGTCTTGACTCATAGGGGCGGACTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033022
• Insert Size: 550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033022.2, NP_148982.1
• RefSeq Size: 615 bp
• RefSeq ORF: 393 bp
• Locus ID: 6229
• Cytogenetics: 10q22.3
• Domains: Ribosomal_S24e
• Protein Pathways: Ribosome
• Gene Summary: 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S24E family of ribosomal proteins. It is located in the cytoplasm. Multiple transcript variants encoding different isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Mutations in this gene result in Diamond-Blackfan anemia. [provided by RefSeq, Nov 2008]'
  Transcript Variant: This variant (a) uses an alternate splice pattern in the 3' coding region, compared to variant d. The resulting protein (isoform a) has a shorter and distinct C-terminus, compared to isoform d.