CXCR5 (NM_032966) Human Untagged Clone

CAT#: SC102158

CXCR5 (untagged)-Human chemokine (C-X-C motif) receptor 5 (CXCR5), transcript variant 2


  "NM_032966" in other vectors (6)

Reconstitution Protocol

USD 660.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CXCR5
Synonyms BLR1; CD185; MDR15
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_032966 edited
CCTCTCAACATAAGACAGTGACCAGTCTGGTGACTCACAGCCGGCACAGCCATGAACTAC
CCGCTAACGCTGGAAATGGACCTCGAGAACCTGGAGGACCTGTTCTGGGAACTGGACAGA
TTGGACAACTATAACGACACCTCCCTGGTGGAAAATCATCTCTGCCCTGCCACAGAGGGG
CCCCTCATGGCCTCCTTCAAGGCCGTGTTCGTGCCCGTGGCCTACAGCCTCATCTTCCTC
CTGGGCGTGATCGGCAACGTCCTGGTGCTGGTGATCCTGGAGCGGCACCGGCAGACACGC
AGTTCCACGGAGACCTTCCTGTTCCACCTGGCCGTGGCCGACCTCCTGCTGGTCTTCATC
TTGCCCTTTGCCGTGGCCGAGGGCTCTGTGGGCTGGGTCCTGGGGACCTTCCTCTGCAAA
ACTGTGATTGCCCTGCACAAAGTCAACTTCTACTGCAGCAGCCTGCTCCTGGCCTGCATC
GCCGTGGACCGCTACCTGGCCATTGTCCACGCCGTCCATGCCTACCGCCACCGCCGCCTC
CTCTCCATCCACATCACCTGTGGGACCATCTGGCTGGTGGGCTTCCTCCTTGCCTTGCCA
GAGATTCTCTTCGCCAAAGTCAGCCAAGGCCATCACAACAACTCCCTGCCACGTTGCACC
TTCTCCCAAGAGAACCAAGCAGAAACGCATGCCTGGTTCACCTCCCGATTCCTCTACCAT
GTGGCGGGATTCCTGCTGCCCATGCTGGTGATGGGCTGGTGCTACGTGGGGGTAGTGCAC
AGGTTGCGCCAGGCCCAGCGGCGCCCTCAGCGGCAGAAGGCAGTCAGGGTGGCCATCCTG
GTGACAAGCATCTTCTTCCTCTGCTGGTCACCCTACCACATCGTCATCTTCCTGGACACC
CTGGCGAGGCTGAAGGCCGTGGACAATACCTGCAAGCTGAATGGCTCTCTCCCCGTGGCC
ATCACCATGTGTGAGTTCCTGGGCCTGGCCCACTGCTGCCTCAACCCCATGCTCTACACT
TTCGCCGGCGTGAAGTTCCGCAGTGACCTGTCGCGGCTCCTGACGAAGCTGGGCTGTACC
GGCCCTGCCTCCCTGTGCCAGCTCTTCCCTAGCTGGCGCAGGAGCAGTCTCTCTGAGTCA
GAGAATGCCACCTCTCTCACCACGTTCTAGGTCCCAGTGTCCCCTTTTATTGCTGCTTTT
CCTTGGGGCAGGCAGTGATGCTGGATGCTCCTTCCAACAGGAGCTGGGATCCTAAGGGCT
CACCGTGGCTAAGAGTGTCCTAGGAGTATCCTCATTTGGGGTAGCTAGAGGAACCAACCC
CCATTTCTAGAACATCCCTGCCAGCTCTTCTGCCGGCCCTGGGGCTAGGCTGGAGCCCAG
GGAGCGGAAAGCAGCTCAAAGGCACAGTGAAGGCTGTCCTTACCCATCTGCACCCCCCTG
GGCTGAGAGAACCTCACGCACCTCCCATCCTAATCATCCAATGCTCAAGAAACAACTTCT
ACTTCTGCCCTTGCCAACGGAGAGCGCCTGCCCCTCCCAGAACACACTCCATCAGCTTAG
GGGCTGCTGACCTCCACAGCTTCCCCTCTCTCCTCCTGCCCACCTGTCAAACAAAGCCAG
AAGCTGAGCACCAGGGGATGAGTGGAGGTTAAGGCTGAGGAAAGGCCAGCTGGCAGCAGA
GTGTGGCCTTCGGACAACTCAGTCCCTAAAAACACAGACATTCTGCCAGGCCCCCAAGCC
TGCAGTCATCTTGACCAAGCAGGAAGCTCAGACTGGTTGAGTTCAGGTAGCTGCCCCTGG
CTCTGACCGAAACAGCGCTGGGTCCACCCCATGTCACCGGATCCTGGGTGGTCTGCAGGC
AGGGCTGACTCTAGGTGCCCTTGGAGGCCAGCCAGTGACCTGAGGAAGCGTGAAGGCCGA
GAAGCAAGAAAGAAACCCGACAGAGGGAAGAAAAGAGCTTTCTTCCCGAACCCCAAGGAG
GGAGATGGATCAATCAAACCCGGCGGTCCCCTCCGCCAGGCGAGATGGGGTGGGGTGGAG
AACTCCTAGGGTGGCTGGGTCCAGGGGATGGGAGGTTGTGGGCATTGATGGGGAAGGAGG
CTGGCTTGTCCCCTCCTCACTCCCTTCCCATAAGCTATAGACCCGAGGAAACTCAGAGTC
GGAACGGAGAAAGGTGGACTGGAAGGGGCCCGTGGGAGTCATCTCAACCATCCCCTCCGT
GGCATCACCTTAGGCAGGGAAGTGTAAGAAACACACTGAGGCAGGGAAGTCCCCAGGCCC
CAGGAAGCCGTGCCCTGCCCCCGTGAGGATGTCACTCAGATGGAACCGCAGGAAGCTGCT
CCGTGCTTGTTTGCTCACCTGGGGTGTGGGAGGCCCGTCCGGCAGTTCTGGGTGCTCCCT
ACCACCTCCCCAGCCTTTGATCAGGTGGGGAGTCAGGGACCCCTGCCCTTGTCCCACTCA
AGCCAAGCAGCCAAGCTCCTTGGGAGGCCCCACTGGGGAAATAACAGCTGTGGCTCACGT
GAGAGTGTCTTCACGGCAGGACAACGAGGAAGCCCTAAGACGTCCCTTTTTTCTCTGAGT
ATCTCCTCGCAAGCTGGGTAATCGATGGGGGAGTCTGAAGCAGATGCAAAGAGGCAAGAG
GCTGGATTTTGAATTTTCTTTTTAATAAAAAGGCACCTATAAAACAGGTCAATACAGTAC
AGGCAGCACAGAGACCCCCGGAACAAGCCTAAAAATTGTTTCAAAATAAAAACCAAGAAG
ATGTCTTC
>OriGene 5' read for NM_032966 unedited
TCGGGATTTGNTATACGACTCACTATAGGGCGGCACGCGAATTCGCACCAGGAAGCCTCT
CAACATAAGACAGTGACCAGTCTGGTGACTCACATCCGGCACAGCCATGAACTACCCGCT
AACGCTGGAAATGGACCTCGAGAACCTGGAGGACCTGTTCTGGGAACTGGACAGATTGGA
CAACTATAACGACACCTCCCTGGTGGAAAATCATCTCTGCCCTGCCACAGAGGGGCCCCT
CATGGCCTCCTTCAAGGCCGTGTTCGTGCCCGTGGCCTACAGCCTCATCTTCCTCCTGGG
CGTGATCGGCAACGTCCTGGTGCTGGTGATCCTGGAGCGGCACCGGCAGACACGCAGTTC
CACGGAGACCTTCCTGTTCCACCTGGCCGTGGCCGACCTCCTGCTGGTCTTCATCTTGCC
CTTTGCCGTGGCCGATGGCTCTGTGGGCTGGGTCCTGGGGACCTTCCTCTGCAAAACTGT
GATTGCCCTGCACAAAGTCAACTTCTACTGCAGCAGCCTGCTCCTGGCCTGCATCGCCGT
GGACCGCTACCTGGCCATTGTCCACGCCGTCCATGCCTACCGCCACCGNCGNCTCCTCTC
CATCCACATCACCTGTGGGACCATCTGGCTGGTGGGCTTCCTCCTTGCCTTGCCAGAGAT
TCTCTTCGCCAAAGTCAGCCAAAGCCATCACAACAACTCCCTGCCACGTTGCACCTTTCT
CCAAGATATCCAAGCAGAAACGCATGCCTGGNTCACCTTCCGATTCCTCTACCATGTGGC
GGGATTCCTGCCTGCCATGCTGGTGATGGGCTGGTGCTACCTGGGATTAAAGCACAGGGT
GCGCCAAGTCCATCGGCGCCCTCATCGGAGAAAGCAGTCAAGGTGGCATTCTGTAGAAAA
CATTTCTTCTTTGCTGTTCACCTACACCATTGTATTTCCTGGAACCTGCGAAGCTGTAGC
CCGGGACAATCCTGCAGCTGAATGCCTTTCCCCGG
Restriction Sites Please inquire     
ACCN NM_032966
Insert Size 2800 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_032966.1, NP_116743.1
RefSeq Size 2893 bp
RefSeq ORF 984 bp
Locus ID 643
Cytogenetics 11q23.3
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Chemokine signaling pathway, Cytokine-cytokine receptor interaction
Gene Summary 'This gene encodes a multi-pass membrane protein that belongs to the CXC chemokine receptor family. It is expressed in mature B-cells and Burkitt's lymphoma. This cytokine receptor binds to B-lymphocyte chemoattractant (BLC), and is involved in B-cell migration into B-cell follicles of spleen and Peyer patches. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]'
Transcript Variant: This variant (2) differs at the 5' end compared to variant 1. This results in translation initiation from an in-frame downstream AUG, and an isoform (2) with a shorter N-terminus compared to isoform 1.

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