RPL35A (AK055653) Human Untagged Clone

CAT#: SC102349

(untagged)-Human cDNA FLJ31091 fis, clone IMR321000155, highly similar to 60S RIBOSOMAL PROTEIN L35A

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL35A
• Synonyms: DBA5|L35A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK055653, the custom clone sequence may differ by one or more nucleotides
  
  
  TACAAAACTCAAATGTTAAATTTGTCTGAAAAATATATTTCTACATGATCTGATAAAGTTCAGATAAGGG
  GCATTCTGTTCCTAAGAATGTCCTAAAAATGGAAAGCTGATAAAAGATTACGCTTAGAATTATACAGGTA
  AAAGACCTACAACAGTTGTTCTCTTGTTTTGATCCCATAGGTTATTTGTTATTACTCTTCTTTAACAGAG
  TGATTTTCTATTCTTAACCTTCTTAAGAGCCAATACAGAGTAGAAAGAAATATTTTCAGAAAGCCTTTTT
  AATAGTAACTTAATGTAAAAATTGTTTATCCTTTGGTTGAGGTTTCCCATTTCCATTTTTAAGCTTATCT
  GAACTGTGAACATGAAAAACGTAATGATTCATTGGTCAAGAAGCTACCATTTGAGGGCTCTAAAGTTTCT
  TTTAGATTTCCTTTTTTATATCAGTACAATTAAAATTTGATTTATCAAGTAGCAGCAGCTTCTTGATATT
  GTTACATACATTAGCTTGTTCATAAAATGTTAGAGTTAAAACTCATTAAAAATGCTATCGTTAAACATAA
  ACTAATTAAAATTCAGTATACGAGATTCTCCCTTCAAAAACATCTTATTTTTATTTTATTTAGTACTTGC
  TTCAGTGTTTTCTAGGAATATCTAGTAAAGGCCTTTACTGTTTTCCACATGGCCTAATTTTATATTGGAC
  TACAATTTTTGAGAAGGAATGCCAAATACAAAATTTTGAATCAATAAATTACAATTATAATTTTTATACT
  TATTTTCAAAGTTAATTTACTTTAGATGGCAGTAAAATCACTGAACTTCTGATTGAAAGAAAATTTTTTA
  GTTTGAAATTTCCTTCTGGAAAGGGGTTTGCAGTCCCTTTGGAAAGGAGTAAGCAATCCTGGAAGTAATT
  GAGTATTTAATCCTCCTATATTTTTTCTGAAGATTTTTGTGTCAGTATACTTTCTTTATGCTGGCTAGAA
  AGATCAGTTCTCAAGAGTTGCAGGATAAAATCAAATTAACTTTCATGTTTCAGAACTAAAACATATCCTT
  GCATTTATGGGAAGGATAGGTAAAAATGGAGATGGTGGCTGATAAAATAAAAATCTTTGAAACTATGTTT
  TTACAAGCCCAAAGCCTTTTGGAGTTGAAAGTATTAAAAAAAAGTATGATATGCTATCTTCAGGTTATGT
  ATATGATCACAGATAATAGCTCTGATGGTCACAGAGCTTGCTGATGGCTTCCAAAGGATGGCTCCTGCCT
  TTTACCTTGTTACCACAGCTCCTCATAGGGCACTTTGAGTGGCAGTTCTGGAATCTGAAAAAGAAAGGAT
  AGTGTAAGAATGGTGGTAAAATAAAAAGAACTGTCTTTATAGTTCCTGCTTTATAGGCTCAATAAGGATT
  TTTCTCTTACCGCCATCTTGGCTCCTGTGGAGGCCTGCTGGGAACGGGACTTCTAAAAGGAACTATGTCT
  GGAAGGCTGTGGTCCAAGGCCATTTTTGCTGGCTATAAGCGGGGTCTCCGGAACCAAAGGGAGCACACAG
  CTCTTCTTAAAATTGAAGGTGTTTACGCCCGAGATGAAACAGAATTCTATTTGGGCAAGAGATGCGCTTA
  TGTATATAAAGCAAAGAACAACACAGTCACTCCTGGCGGCAAACCAAACAAAACCAGAGTCATCTGGGGA
  AAAGTAACTCGGGCCCATGGAAACAGTGGCATGGTTCGTGCCAAATTCCGAAGCAATCTTCCTGCTAAGG
  CCATTGGACACAGAATCCGAGTGATGCTGTACCCCTCAAGGATTTAAACTAACGAAAAGTCAATAAATAA
  ATGTGGATTTGTGCTCTTGTATTTTTAAGTGGATTAAAAAACTTACTACCTT
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK055653 unedited
  CGGCCGCGAATTCGGCACCAGGCCATCTTGGCTCCTGTGGAGGCCTGCTGGGAACGGGAC
  TTCTAAAAGGAACTATGTCTGGAAGGCTGTGGTCCAAGGCCATTTTTGCTGGCTATAAGC
  GGGGTCTCCGGAACCAAAGGGAGCACACAGCTCTTCTTAAAATTGAAGGTGTTTACGCCC
  GAGATGAAACAGAATTCTATTTGGGCAAGAGATGCGCTTATGTATATAAAGCAAAGAACA
  ACACAGTCACTCCTGGCGGCAAACCAAACAAAACCAGAGTCATCTGGGGAAAAGTAACTC
  GGGCCCATGGAAACAGTGGCATGGTTCGTGCCAAATTCCGAAGCAATCTTCCTGCTAAGG
  CCATTGGACACAGAATCCGAGTGATGCTGTACCCCTCAAGGATTTAAACTAACGAAAAAT
  CAATAAATAAATGTGGATTTGTGCTCTTGTATTTTTAAGTGGATTAAAAAACTTACTACC
  TTAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCT
  GAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAG
  TTGCCACTCCAGTGCCCACCAGCCTTGTCCTTAATAAATTAAGTTGCATCATTTTGTCTG
  ACTAGGTGTCCTCTTAATATAATGGGTGAGGGGGGGGGTTTTTTTAAACCAGGGCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK055653 unedited
  NTCCGGTTCATCTATGNNACCGCGGCCGCATNCTAGNGATCGGTTTTTTTTTTTTTTTTT
  TTAAGGTAGTAAGTTTTTTAATCCACTTAAAATACAAGAGCACAAATCCACATTTATTTA
  TTGATTTTTCGTTAGTTTAAATCCTTGAGGGGTACAGCATCACTCGGATTCTGTGTCCAA
  TGGCCTTAGCAGGAAGATTGCTTCGGAATTTGGCACGAACCATGCCACTGTTTCCATGGG
  CCCGAGTTACTTTTCCCCAGATGACTCTGGTTTTGTTTGGTTTGCCGCCAGGAGTGACTG
  TGTTGTTCTTTGCTTTATATACATAAGCGCATCTCTTGCCCAAATAGAATTCTGTTTCAT
  CTCGGGCGTAAACACCTTCAATTTTAAGAAGAGCTGTGTGCTCCCTTTGGTTCCGGAGAC
  CCCGCTTATAGCCAGCAAAAATGGCCTTGGACCACAGCCTTCCAGACATAGTTCCTTTTA
  GAAGTCCCGTTCCCAGCAGGCCTCCACAGGAGCCAAGATGGCCTGGTGCCGAATTCGCGG
  CCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTAT
  ATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGCGGGGTTATTACG
  ACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGG
  GTGGAGACTTGGAAATCCCCGTGAGTCANACCGCTATCCACGCCCATTGGTGTACTGCCA
  AAACCGCATCACCATGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTANGAAA
  GTCCCGNTAGGTCATGTACTGGGCATAAATGCCAGCGGGCCATTTACCGTCATTGACA
  
• Restriction Sites: NotI-NotI
• ACCN: AK055653
• Insert Size: 500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK055653.1
• RefSeq Size: 1872 bp
• RefSeq ORF: 1872 bp
• Locus ID: 6165
• Cytogenetics: 3q29
• Domains: Ribosomal_L35Ae
• Protein Pathways: Ribosome
• Gene Summary: 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L35AE family of ribosomal proteins. It is located in the cytoplasm. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome. Although this gene was originally mapped to chromosome 18, it has been established that it is located at 3q29-qter. Alternative splicing results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Oct 2015]'