RPL35A (AK055653) Human Untagged Clone

CAT#: SC102349

(untagged)-Human cDNA FLJ31091 fis, clone IMR321000155, highly similar to 60S RIBOSOMAL PROTEIN L35A


Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol RPL35A
Synonyms DBA5|L35A
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for AK055653, the custom clone sequence may differ by one or more nucleotides


TACAAAACTCAAATGTTAAATTTGTCTGAAAAATATATTTCTACATGATCTGATAAAGTTCAGATAAGGG
GCATTCTGTTCCTAAGAATGTCCTAAAAATGGAAAGCTGATAAAAGATTACGCTTAGAATTATACAGGTA
AAAGACCTACAACAGTTGTTCTCTTGTTTTGATCCCATAGGTTATTTGTTATTACTCTTCTTTAACAGAG
TGATTTTCTATTCTTAACCTTCTTAAGAGCCAATACAGAGTAGAAAGAAATATTTTCAGAAAGCCTTTTT
AATAGTAACTTAATGTAAAAATTGTTTATCCTTTGGTTGAGGTTTCCCATTTCCATTTTTAAGCTTATCT
GAACTGTGAACATGAAAAACGTAATGATTCATTGGTCAAGAAGCTACCATTTGAGGGCTCTAAAGTTTCT
TTTAGATTTCCTTTTTTATATCAGTACAATTAAAATTTGATTTATCAAGTAGCAGCAGCTTCTTGATATT
GTTACATACATTAGCTTGTTCATAAAATGTTAGAGTTAAAACTCATTAAAAATGCTATCGTTAAACATAA
ACTAATTAAAATTCAGTATACGAGATTCTCCCTTCAAAAACATCTTATTTTTATTTTATTTAGTACTTGC
TTCAGTGTTTTCTAGGAATATCTAGTAAAGGCCTTTACTGTTTTCCACATGGCCTAATTTTATATTGGAC
TACAATTTTTGAGAAGGAATGCCAAATACAAAATTTTGAATCAATAAATTACAATTATAATTTTTATACT
TATTTTCAAAGTTAATTTACTTTAGATGGCAGTAAAATCACTGAACTTCTGATTGAAAGAAAATTTTTTA
GTTTGAAATTTCCTTCTGGAAAGGGGTTTGCAGTCCCTTTGGAAAGGAGTAAGCAATCCTGGAAGTAATT
GAGTATTTAATCCTCCTATATTTTTTCTGAAGATTTTTGTGTCAGTATACTTTCTTTATGCTGGCTAGAA
AGATCAGTTCTCAAGAGTTGCAGGATAAAATCAAATTAACTTTCATGTTTCAGAACTAAAACATATCCTT
GCATTTATGGGAAGGATAGGTAAAAATGGAGATGGTGGCTGATAAAATAAAAATCTTTGAAACTATGTTT
TTACAAGCCCAAAGCCTTTTGGAGTTGAAAGTATTAAAAAAAAGTATGATATGCTATCTTCAGGTTATGT
ATATGATCACAGATAATAGCTCTGATGGTCACAGAGCTTGCTGATGGCTTCCAAAGGATGGCTCCTGCCT
TTTACCTTGTTACCACAGCTCCTCATAGGGCACTTTGAGTGGCAGTTCTGGAATCTGAAAAAGAAAGGAT
AGTGTAAGAATGGTGGTAAAATAAAAAGAACTGTCTTTATAGTTCCTGCTTTATAGGCTCAATAAGGATT
TTTCTCTTACCGCCATCTTGGCTCCTGTGGAGGCCTGCTGGGAACGGGACTTCTAAAAGGAACTATGTCT
GGAAGGCTGTGGTCCAAGGCCATTTTTGCTGGCTATAAGCGGGGTCTCCGGAACCAAAGGGAGCACACAG
CTCTTCTTAAAATTGAAGGTGTTTACGCCCGAGATGAAACAGAATTCTATTTGGGCAAGAGATGCGCTTA
TGTATATAAAGCAAAGAACAACACAGTCACTCCTGGCGGCAAACCAAACAAAACCAGAGTCATCTGGGGA
AAAGTAACTCGGGCCCATGGAAACAGTGGCATGGTTCGTGCCAAATTCCGAAGCAATCTTCCTGCTAAGG
CCATTGGACACAGAATCCGAGTGATGCTGTACCCCTCAAGGATTTAAACTAACGAAAAGTCAATAAATAA
ATGTGGATTTGTGCTCTTGTATTTTTAAGTGGATTAAAAAACTTACTACCTT


>OriGene 5' read for AK055653 unedited
CGGCCGCGAATTCGGCACCAGGCCATCTTGGCTCCTGTGGAGGCCTGCTGGGAACGGGAC
TTCTAAAAGGAACTATGTCTGGAAGGCTGTGGTCCAAGGCCATTTTTGCTGGCTATAAGC
GGGGTCTCCGGAACCAAAGGGAGCACACAGCTCTTCTTAAAATTGAAGGTGTTTACGCCC
GAGATGAAACAGAATTCTATTTGGGCAAGAGATGCGCTTATGTATATAAAGCAAAGAACA
ACACAGTCACTCCTGGCGGCAAACCAAACAAAACCAGAGTCATCTGGGGAAAAGTAACTC
GGGCCCATGGAAACAGTGGCATGGTTCGTGCCAAATTCCGAAGCAATCTTCCTGCTAAGG
CCATTGGACACAGAATCCGAGTGATGCTGTACCCCTCAAGGATTTAAACTAACGAAAAAT
CAATAAATAAATGTGGATTTGTGCTCTTGTATTTTTAAGTGGATTAAAAAACTTACTACC
TTAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCT
GAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAG
TTGCCACTCCAGTGCCCACCAGCCTTGTCCTTAATAAATTAAGTTGCATCATTTTGTCTG
ACTAGGTGTCCTCTTAATATAATGGGTGAGGGGGGGGGTTTTTTTAAACCAGGGCCA
>OriGene 3' read for AK055653 unedited
NTCCGGTTCATCTATGNNACCGCGGCCGCATNCTAGNGATCGGTTTTTTTTTTTTTTTTT
TTAAGGTAGTAAGTTTTTTAATCCACTTAAAATACAAGAGCACAAATCCACATTTATTTA
TTGATTTTTCGTTAGTTTAAATCCTTGAGGGGTACAGCATCACTCGGATTCTGTGTCCAA
TGGCCTTAGCAGGAAGATTGCTTCGGAATTTGGCACGAACCATGCCACTGTTTCCATGGG
CCCGAGTTACTTTTCCCCAGATGACTCTGGTTTTGTTTGGTTTGCCGCCAGGAGTGACTG
TGTTGTTCTTTGCTTTATATACATAAGCGCATCTCTTGCCCAAATAGAATTCTGTTTCAT
CTCGGGCGTAAACACCTTCAATTTTAAGAAGAGCTGTGTGCTCCCTTTGGTTCCGGAGAC
CCCGCTTATAGCCAGCAAAAATGGCCTTGGACCACAGCCTTCCAGACATAGTTCCTTTTA
GAAGTCCCGTTCCCAGCAGGCCTCCACAGGAGCCAAGATGGCCTGGTGCCGAATTCGCGG
CCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTAT
ATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGCGGGGTTATTACG
ACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGG
GTGGAGACTTGGAAATCCCCGTGAGTCANACCGCTATCCACGCCCATTGGTGTACTGCCA
AAACCGCATCACCATGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTANGAAA
GTCCCGNTAGGTCATGTACTGGGCATAAATGCCAGCGGGCCATTTACCGTCATTGACA
Restriction Sites NotI-NotI     
ACCN AK055653
Insert Size 500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq AK055653.1
RefSeq Size 1872 bp
RefSeq ORF 1872 bp
Locus ID 6165
Cytogenetics 3q29
Domains Ribosomal_L35Ae
Protein Pathways Ribosome
Gene Summary 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L35AE family of ribosomal proteins. It is located in the cytoplasm. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome. Although this gene was originally mapped to chromosome 18, it has been established that it is located at 3q29-qter. Alternative splicing results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Oct 2015]'

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