ESD (AK093643) Human Untagged Clone

CAT#: SC102451

(untagged)-Human cDNA FLJ36324 fis, clone THYMU2005546, highly similar to ESTERASE D (EC 3.1.1.1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ESD
• Synonyms: FGH
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK093643, the custom clone sequence may differ by one or more nucleotides
  
  
  GTCTATTTTTGGCCACTCCATGGGAGGTCATGGAGCTCTGATCTGTGCTTTGAAAAATCCTGGAAAATAC
  AAATCTGTGTCAGCATTTGCTCCAATTTGCAACCCTGTACTCTGTCCCTGGGGCAAAAAAGCCTTTAGTG
  GATATTTGGGAACAGATCAAAGTAAATGGAAGGCTTATGATGCTACCCACCTTGTGAAATCCTATCCAGG
  ATCTCAGCTGGACATACTAATTGATCAAGGGAAAGATGACCAGTTTCTTTTAGATGGACAGTTACTCCCT
  GATAACTTCATAGCTGCCTGTACAGAAAAGAAAATCCCCGTTGTTTTTCGATTGCAAGAGGCAAGTATTA
  GGAAACTTTAGCTTGATCTAGATAATGTAACTATGAAAAATTAGGATTCTGTGATATTTTCTTAACTTTA
  TTTTAGAATATAAAATGCTACTTCTAGTAAGTTTTTAATTATATTCAGACACTTGAAGTGAAAACCTGCC
  TATAAAATTAATTATATCTAACACTAAATTTGATAGAAATAGGAAAGCTTTTGGCAGTGAAAAATATTGT
  TTTCCATCAAACCAGTAAATAACAAGATTTTTAATGCTTGAATGATGATTAATCTTCAGCATTAGCAGAT
  ACTTCTTTCTTTACACATGGACCTTTGCTGCAGTAGAATAAAAAGGACAAACTTCTACCTAAATTACTCC
  TTATTTTATGTATATATTTTAGCCTCAGAAGTTAAGTATTATTTATCACACATGTGCTTTATATTATTTG
  ACTTTCATATTAATCTATTCATTGTGCTACTTGATATTTCTAGTTGTTTAAGAATAATTACTGAATCCCA
  ATGGGCTTGCAAGTTTTTTTTAACTAAAATATTTTTGACCGTCCACTACTCACCTAACTCTATATATGCT
  ACCATAGTCCTAATTAAGGAAGTTCTGCTTTTATTTTTAAGAAAAATTCATAAGCTTCAGAATATAATTT
  AAAATATTTTAGAGTGAAAAATGAGCACTGAAAATTATACATGGTCATGATTTTTAAATAAATTTTTATC
  CATGTTTAAGGATAATGTGTCAGGCACATTGATCTAAAATGTAATTACAATGGTAAATGTTCAAATCTAG
  ATCAGTGTGTAATGTGGTACATTAGTTTGTAAAATGCTTTCACAGCATTAACTCATTGTTAATTCTTATA
  CCGCCAAATGAGATATCATTTCTTTCCTATCCATCTGTTTTACAAATGAGGAGACAGGCTCAGAGAAGTA
  ACCTACTTATCTAAGGCCACATAGCTAGTAAGTGGTGGAACCAGAACTGGAACTAAGGCCATTTAACTGC
  AAATACTGACCTCCTGTCATTTATCAAGCCATTTTCAGACGGCATGATGTTCTCCTGAGGAGCAAAGGAA
  TGGAATTAGAGCTAGCACAAGGAGAAATTGTGCATAGCTTGCTGCCTACGTAACTTATGCAGAAGATCTG
  AGCAGTCTTCCTGTTTTTAGGGATATTTTGCACATGTGAACTATATAGTGAGAAAATGTCAAAAACTAAT
  ACATGATATACTGTTTGTCTCTTAGTGTCCATCCTTGAGTAATAGAATTTTGTTTTAGATTTATTTGAAC
  ATTTACCATCACAATTAAATTTTAGGTCAACAACCAGCCTTGAGAAATCCAAATACCAATTTGACTATAA
  ATATAGTAATACGTTTAACTGCAATAACTATAGCTATAAGTGCGTACCAAACCATGTTTTTTCCAAATGA
  CCTTAATTTCTTGGCACTACCCATGTTGGATACCTCATTTGCTTCCCCAAAGACTGTGCCCAAAATTTCT
  GTTGTTCCTACAATGATTCTGCCTGCCAACAAAGTCTGAAAATCTCTCAAGTTTGTTATAAAAACAGCAC
  AGTTAGAACATGATGACATTCCTGGAGGGAAAAATAAGTCCACTTATTTGTTTCTAAAATGCCTGTATCC
  CAAGCAACTAGAACACATAGTTGGCTATACTTAATGAGGAGTCAAGACAAACCATTCAATTCTTCTAAAA
  AAGTAAGAAAGTATTCAAAGCACCAGAGTCACAATGTTGAGCTCAGGTGCAATTTTCTATTCACTTTACT
  TCCAGCATGGTCGGGTTAAGAGTGGACTACATAGCTTGAAGCGGATTTCGTTGAGATTCGTTGTAATTGA
  ATCAACAACTGAATGAGCATGCCTAAGTAGTGGCTCCAGAGAGTCTGCTTTCAGATTTTCTTGCCAGCTT
  TGACCCAAGATTACCCTGGGTCTGCGAGTTTTATCACCTGCTTTATCATTTGATTACAAGTATAGTATAA
  CTGTATTTCCTTGTGGGATAGAAGCTGTAAAATAAGCTGAAAATAATAGGGGATATATATAAATAAACGA
  TCTGAGAAAGAATAAAGAAAATAATTAC
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK093643 unedited
  CGGCCGCGAATTCGGCACGAGCTATTTTTGGCCACTCCATGGGAGGTCATGGAGCTCTGA
  TCTGTGCTTTGAAAAATCCTGGAAAATACAAATCTGTGTCAGCATTTGCTCCAATTTGCA
  ACCCTGTACTCTGTCCCTGGGGCAAAAAAGCCTTTAGTGGATATTTGGGAACAGATCAAA
  GTAAATGGAAGGCTTATGATGCTACCCACCTTGTGAAATCCTATCCAGGATCTCAGCTGG
  ACATACTAATTGATCAAGGGAAAGATGACCAGTTTCTTTTAGATGGACAGTTACTCCCTG
  ATAACTTCATAGCTGCCTGTACAGAAAAGAAAATCCCCGTTGTTTTTCGATTGCAAGAGG
  CAAGTATTAGGAAACTTTAGCTTGATCTAGATAATGTAACTATGAAAAATTAGGATTCTG
  TGATATTTTCTTAACTTTATTTTAGAATATAAAATGCTACTTCTAGTAAGTTTTTAATTA
  TATTCAGACACTTGAAGTGAAAACCTGCCTATAAAATTAATTATATCTAACACTAAATTT
  GATAGAAATAGGAAAGCTTTTGGCAGTGAAAAATATTGTTTTCCATCAAACCAGTAAATA
  ACAAGATTTTTAATGCTTGAATGATGATTAATCTTCAGCATTAGCAGATACTTCTTTCTT
  TACACATGGACCTTTGCTGCAGTAGAATAAAAGGACAAACTTCTACCTAAATTACTCCTT
  ATTNTATGTATATATTNTAGN
  
• Restriction Sites: NotI-NotI
• ACCN: AK093643
• Insert Size: 6000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK093643.1
• RefSeq Size: 2408 bp
• RefSeq ORF: 2408 bp
• Locus ID: 2098
• Cytogenetics: 13q14.2
• Gene Summary: 'This gene encodes a serine hydrolase that belongs to the esterase D family. The encoded enzyme is active toward numerous substrates including O-acetylated sialic acids, and it may be involved in the recycling of sialic acids. This gene is used as a genetic marker for retinoblastoma and Wilson's disease. [provided by RefSeq, Feb 2009]'