HARS2 (NM_012208) Human Untagged Clone

CAT#: SC102514

HARS2 (untagged)-Human histidyl-tRNA synthetase 2, mitochondrial (putative) (HARS2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HARS2
• Synonyms: HARSL; HARSR; HisRS; HO3; PRLTS2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC102514 sequence for NM_012208 edited (data generated by NextGen Sequencing)
  ATGCCCCTGCTCGGACTTCTTCCCAGGAGGGCCTGGGCTTCGCTGCTCAGCCAGCTCCTG
  CGACCGCCCTGCGCTTCGTGCACCGGGGCGGTCCGTTGCCAAAGCCAGGTTGCAGAGGCA
  GTGTTAACATCCCAACTGAAAGCACATCAAGAGAAACCAAATTTTATTATCAAGACCCCA
  AAGGGTACCAGGGATCTTAGTCCTCAGCATATGGTTGTGAGGGAGAAAATTCTTGATTTG
  GTTATCAGCTGCTTTAAACGTCATGGAGCAAAGGGGATGGACACCCCAGCATTTGAGCTG
  AAGGAAACCCTGACTGAGAAGTATGGGGAGGACTCTGGGCTCATGTATGATCTGAAGGAT
  CAAGGTGGAGAGCTGTTGTCCCTCCGCTATGACCTTACTGTTCCCTTTGCTCGTTATCTG
  GCCATGAATAAGGTGAAGAAGATGAAACGTTATCATGTTGGAAAGGTGTGGCGGCGAGAG
  AGCCCAACCATAGTCCAAGGCCGTTATAGGGAGTTCTGCCAGTGTGATTTTGACATTGCT
  GGTCAGTTTGACCCTATGATCCCCGATGCAGAGTGTTTGAAGATCATGTGTGAAATCCTA
  AGTGGATTGCAGTTGGGAGACTTTCTCATTAAGGTAAATGACCGGCGGATTGTGGATGGG
  ATGTTTGCTGTCTGTGGTGTTCCTGAAAGCAAGTTCCGTGCCATCTGCTCCTCCATAGAT
  AAACTAGACAAGATGGCTTGGAAAGATGTGAGACATGAGATGGTGGTGAAGAAAGGCCTG
  GCTCCTGAGGTGGCTGATCGAATTGGGGACTATGTCCAGTGTCATGGTGGGGTATCCCTA
  GTAGAGCAAATGTTTCAGGATCCCAGACTATCCCAGAACAAGCAGGCCCTGGAGGGCCTG
  GGAGACCTAAAGCTGCTATTTGAATACCTGACTTTATTTGGAATTGCTGATAAGATCTCC
  TTTGACCTCAGCCTGGCTCGGGGCCTAGACTACTATACAGGAGTGATCTATGAAGCAGTG
  CTGCTGCAGACCCCAACTCAGGCTGGGGAGGAGCCCCTGAATGTGGGCAGTGTGGCTGCT
  GGTGGGCGCTATGATGGGCTGGTGGGCATGTTTGACCCCAAGGGCCACAAGGTGCCATGT
  GTGGGACTCAGCATTGGGGTTGAGCGAATCTTCTACATTGTGGAGCAGAGGATGAAGACC
  AAAGGTGAGAAGGTGCGGACTACAGAGACTCAAGTGTTTGTGGCCACACCACAGAAGAAC
  TTTCTCCAAGAACGGTTGAAGCTTATTGCAGAGCTTTGGGATTCTGGAATCAAGGCAGAG
  ATGCTATACAAGAACAACCCCAAACTATTAACCCAGCTGCACTATTGTGAGAGCACAGGC
  ATTCCACTGGTGGTCATTATTGGTGAGCAAGAACTGAAAGAAGGGGTCATCAAGATCCGT
  TCAGTGGCCAGCAGAGAGGAGGTGGCCATTAAACGGGAAAATTTTGTGGCTGAAATTCAG
  AAGCGACTGTCTGAGTCTTGA
  
  Clone variation with respect to NM_012208.2
  327 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012208 unedited
  GCATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGGCTACTA
  AGGGAACTTGGGAGGATCCCACCTCAGCCTTCGTGACTAGTGAGGTGCGCAAACGCCCGA
  GTTTTCCCTGGTGCGCGGGTTCCGCCTTTGCAGTGCCCTCCACCCTTCCTGGTGTCTGAC
  CCGCCTCCTTCCCAGGCCTTTTGTTCCTGTCCCGGAAAGCCGGCGTCCTGCCGCGCGATG
  CCCCTGCTCGGACTTCTTCCCAGGAGGGCCTGGGCTTCGCTGCTCAGCCAGCTCCTGCGA
  CCGCCCTGCGCTTCGTGCACCGGGGCGGTCCGTTGCCAAAGCCAGGTTGCAGAGGCAGTG
  TTAACATCCCAACTGAAAGCACATCAAGAGAAACCAAATTTTATTATCAAGACCCCAAAG
  GGTACCAGGGATCTTAGTCCTCAGCATATGGTTGTGAGGGAGAAAATTCTTGATTTGGTT
  ATCAGCTGCTTTAAACGTCATGGAGCAAAGGGGATGGACACCCCAGCATTTGAGCTGAAG
  GAAACCCTGACTGAGAAGTATGGGGAGGACTCTGGGCTCATGTATGATCTGAAGGATCAA
  GGTGGAGAGCTGTTGTCCCTCCGCTATGACCTTACTGTTCCCTTTGCTCGTTATCTGGCC
  ATGAATAAGGTGAAGAAGATGAAACGTTATCATGTTGGAAAGGTGTGGCGGCGAGAGAGC
  CCAACCATAGTCCAAGGCCGTTATAGGGAGTTCTGCCAGTGTGATTNTGACATTGCTGGT
  CAGTTTGACCCTATGATCCCCGATGCAGAGTGGTTGAAGATCATGTGTGAAATCCTAAGT
  GGATTGCAGTTGGGAGACTNTCTCATTAANGGTAATGACCNGCNGATNGTGNATGGGGAT
  GTTGCTGTCTTGTGTGTCCTGGAAAGCNAGTNCCGTGCCATCTGCTCTCCTAGATAACTA
  GACAGATGCTGGGAAGATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012208 unedited
  NAAATTTTTGACTNTAGNCCCGCGGNCCCGCTTTCNANNGATCGGTTTTTTTTTTTTTTT
  TTTGCACTTAATATTTTATTTAACATGTTATCCAACCACTTTCTAAAACAGCCTCCATGG
  CACGGTATGGAATAGAGCAGTGATTCTGGCACAGGAAATGGTCTCCAAAATCTCAAAATA
  TCAGATCTCTACCTTGTCAAGTCATCTATTAAGTTCAATGTTCAAGATTATCAAGCATAC
  AGCCCCACTCTTCTACTGTAAGCAATGGATGGGCTAGAGAGATGTCAAATCAACATGGGT
  ACTGCCATGCCCTTAGGATAGCATCTGTCCTCAAAGCTGAAAGCAGCCATTGCCCCTCTT
  TGCTGTCTTCCAAAAACTCCCCAGACAGGCCAATGGCTGATTGTGGACAAATTCACCAGA
  ATCTGGTTCCGTAGCTACAGTAGAAGGCTAAGTATCTGGTTTCTGATCTCAATGGTTCAG
  AATCCTGAGCCTGAGGTGACTCTCCCAGAGCCTTGCTCACAGCAACAATCTCACTACCTC
  TTGACAACGTTCCGTGGCACTTCAAATTTGCCTCTGCAGCTAGAGCATGTCTCTTTCACA
  TCCAGCCATCTGCACCCATGCAGAATAGCTGCTCACACTAGCCCAGGAGTCCTCAGGGTC
  TTCTACAGTTCTGCACCCAGGAAGAATGGAGGAGGCAGAAGGTACTTGTCACCCTTCCTG
  GCTAGTTGTTGTGAAGTCCATCACTCAATTCCAGAGGAATTCAGTTCTAGAGAAAACCTT
  TTCTACAAAGAGCAGCAGATGGGAATCANGCAAGGATCAAGACTCAGACAGTCGCTTCTG
  AATTTCAGCCACAAAATTTNCCCGTTAATGGCCCACTNCTCTCTGCTGGCCACTGNAACG
  GAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012208
• ORF Size: 1521 bp
• Insert Size: 2550
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_012208.2, NP_036340.1
• RefSeq Size: 2508
• RefSeq ORF: 1521
• Locus ID: 23438
• Domains: tRNA-synt_2b, HGTP_anticodon
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Gene Summary: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes likely share a bidirectional promoter. Mutations in this gene are associated with the pathogenesis of Perrault syndrome, which involves ovarian dysgenesis and sensorineural hearing loss. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).