KCNK2 (NM_014217) Human Untagged Clone

CAT#: SC102536

KCNK2 (untagged)-Human potassium channel, subfamily K, member 2 (KCNK2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNK2
• Synonyms: hTREK-1c; hTREK-1e; K2p2.1; TPKC1; TREK; TREK-1; TREK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014217 edited
  ATGGCGGCACCTGACTTGCTGGATCCTAAATCTGCCGCTCAGAACTCCAAACCGAGGCTC
  TCGTTTTCCACGAAACCCACAGTGCTTGCTTCCCGGGTGGAGAGTGACACGACCATTAAT
  GTTATGAAATGGAAGACGGTCTCCACGATATTCCTGGTGGTTGTCCTCTATCTGATCATC
  GGAGCCACCGTGTTCAAAGCATTGGAGCAGCCTCATGAGATTTCACAGAGGACCACCATT
  GTGATCCAGAAGCAAACATTCATATCCCAACATTCCTGTGTCAATTCGACGGAGCTGGAT
  GAACTCATTCAGCAAATAGTGGCAGCAATAAATGCAGGGATTATACCGTTAGGAAACACC
  TCCAATCAAATCAGTCACTGGGATTTGGGAAGTTCCTTCTTCTTTGCTGGCACTGTTATT
  ACAACCATAGGATTTGGAAACATCTCACCACGCACAGAAGGCGGCAAAATATTCTGTATC
  ATCTATGCCTTACTGGGAATTCCCCTCTTTGGTTTTCTCTTGGCTGGAGTTGGAGATCAG
  CTAGGCACCATATTTGGAAAAGGAATTGCCAAAGTGGAAGATACGTTTATTAAGTGGAAT
  GTTAGTCAGACCAAGATTCGCATCATCTCAACAATCATATTTATACTATTTGGCTGTGTA
  CTCTTTGTGGCTCTGCCTGCGATCATATTCAAACACATAGAAGGCTGGAGTGCCCTGGAC
  GCCATTTATTTTGTGGTTATCACTCTAACAACTATTGGATTTGGTGACTACGTTGCAGGT
  GGATCCGATATTGAATATCTGGACTTCTATAAGCCTGTCGTGTGGTTCTGGATCCTTGTA
  GGGCTTGCTTACTTTGCTGCTGTCCTGAGCATGATTGGAGATTGGCTCCGAGTGATATCT
  AAAAAGACAAAAGAAGAGGTGGGAGAGTTCAGAGCACACGCTGCTGAGTGGACAGCCAAC
  GTCACAGCCGAATTCAAAGAAACCAGGAGGCGACTGAGTGTGGAGATTTATGACAAGTTC
  CAGCGGGCCACCTCCATCAAGCGGAAGCTCTCGGCAGAACTGGCTGGAAACCACAATCAG
  GAGCTGACTCCTTGTAGGAGGACCCTGTCAGTGAACCACCTGACCAGCGAGAGGGATGTC
  TTGCCTCCCTTACTGAAGACTGAGAGTATCTATCTGAATGGTTTGACGCCACACTGTGCT
  GGTGAAGAGATTGCTGTGATTGAGAACATCAAATAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014217 unedited
  AATAGGTATACGACTTACTATAGGGCGGCCGCGCATTCGGCACGAGGGTCCAGCCCCGCT
  CTCCCCACCTTGTAAAACAAAGCCGGGGAAAATGCCTGCCCGTGCAGCTCGGAGCGCGCA
  GCCCGTCTCTGAATAAGAATGGCGGCACCTGACTTGCTGGATCCTAAATCTGCCGCTCAG
  AACTCCAAACCGAGGCTCTCGTTTTCCACGAAACCCACAGTGCTTGCTTCCCGGGTGGAG
  AGTGACACGACCATTAATGTTATGAAATGGAAGACGGTCTCCACGATATTCCTGGTGGTT
  GTCCTCTATCTGATCATCGGAGCCACCGTGTTCAAAGCATTGGAGCAGCCTCATGAGATT
  TCACAGAGGACCACCATTGTGATCCAGAAGCAAACATTCATATCCCAACATTCCTGTGTC
  AATTCGACGGAGCTGGATGAACTCATTCAGCAAATAGTGGCAGCAATAAATGCAGGGATT
  ATACCGTTAGGAAACACCTCCAATCAAATCAGTCACTGGGATTTGGGAAGTTCCTTCTTC
  TTTGCTGGCACTGTTATTACAACCATAGGATTTGGAAACATCTCACCACGCACAGAAGGC
  GGNCAAATATTCTGTATCATCTATGCCTTACTGGGAATTCCCCTCTTTGGTTNTCTCTTG
  GCTGGAGTTGGAGATCAGCTANGCACCATATTTGGAAAAGGAAATTGCCAAGTGGAAGAT
  ACGTTTATTAAGTGGAATGTTAGTCAGACCAAGATTCGCATCATCTCAACAAATCATATT
  AACCTATNTNGCTGTGTACTCTTTGTGCTCTGCCTGCGATCATATTCAAACACATGAANG
  CCTGAGTGCCCCTGNACGCCATTATTTTGTGGTTATCACTCTAACAACTATTGGATTNGT
  GACTACCTTGCCAGTGGATCCGAATTGAAAATCTGACTTTATAACCCTGTCCGTGGTTCT
  GAAACCTGTAAGCCTGCTTACTTGCTGCGTNCCGACAGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014217 unedited
  TAGCTTGGCCGCGGCCGCAATCTANGATCNGNCTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCCCTAAAAACCAAACTTTTTTTGG
  CAAAACAATTCAAAGTTTATCCCGGGGCCATTCCCCCATTAAAATTTTCGGAACAGTTTT
  TTGGGGAAAGGGGGGGACCTTTTTTTTTAAAAAACCCCGGCTTTTTAAAATTTGGGGGGG
  ATTTTTTTTAACTTTATAAAAGGCCTTTTAACAAATTAATGGAAATTTTCCCTTTTTAGC
  TAAAATTAAAATTTGGGAAAAAAACCGGCAATAAATTAATTGGCTTTTTTTAAAAAATTG
  GGGTTTTTTTTTCAAAACCCTAATCTGAAGGCCCCTTTTTAAAAAACAGGTCCCCCCCCC
  CCCCCCAAAAAAGGAAAAAAAACCCGTTGGCAATTTTTTTTAGGGAAATTTTTTTTTTTC
  GGCTTTTTAAAAATTTTTTTTTTTTCCCGGGGCCCCCCGACCTAAGTTTGAAAAAAAAGG
  AAGGGGGAGCAATTTCCGGGGGGCCTTTACGGAATTTTTTTTGAACCTGGGCCCCCTCCC
  AAGAATCTCCGGGTTTAAAATTAACCCAAAAAGAAATTCCGCCCCTTTTTATGGGAAAAA
  AACCCCCGCACACCCCCAAAATTTAAATGGGGCTAAACAACTTTTTTCTTAAGGCCAAAA
  AAGAATTATGCCCCGCAATTGGGTTTTTTCCCTGGGGCAGGCCCAGGGGCCCCCCCTTTA
  ATTTTGCGCCTCCCAGCCAGGTACACCTCCCAAAGTTCCCCACCAAAGCCTGGCCTGGGC
  CTCGCCTACCACGGGCCCCCCTTCTCTTTGGGAAAGGCCCCCCGAAAAAAATTTTATCCT
  TTTAACCAAAAACATAAAGCGGGGNAACCGGTTATACAACCCTGAACCTTTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014217
• Insert Size: 3460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014217.2, NP_055032.1
• RefSeq Size: 3198 bp
• RefSeq ORF: 1236 bp
• Locus ID: 3776
• Cytogenetics: 1q41
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'This gene encodes one of the members of the two-pore-domain background potassium channel protein family. This type of potassium channel is formed by two homodimers that create a channel that leaks potassium out of the cell to control resting membrane potential. The channel can be opened, however, by certain anesthetics, membrane stretching, intracellular acidosis, and heat. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR and beginning of the open reading frame compared to variant 1. The resulting isoform (b) is slightly shorter and has a distinct N-terminus compared to isoform a.