NEDD4 2 (NEDD4L) (NM_015277) Human Untagged Clone

CAT#: SC102592

NEDD4L (untagged)-Human neural precursor cell expressed, developmentally down-regulated 4-like (NEDD4L), transcript variant d

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEDD4L
• Synonyms: hNEDD4-2; NEDD4-2; NEDD4.2; PVNH7; RSP5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC102592 sequence for NM_015277 edited (data generated by NextGen Sequencing)
  ATGGCGACCGGGCTCGGGGAGCCGGTCTATGGACTTTCCGAAGACGAGGGAGAGTCCCGT
  ATTCTCAGAGTAAAAGTTGTTTCTGGAATTGATCTCGCCAAAAAGGACATCTTTGGAGCC
  AGTGATCCGTATGTGAAACTTTCATTGTACGTAGCGGATGAGAATAGAGAACTTGCTTTG
  GTCCAGACAAAAACAATTAAAAAGACACTGAACCCAAAATGGAATGAAGAATTTTATTTC
  AGGGTAAACCCATCTAATCACAGACTCCTATTTGAAGTATTTGACGAAAATAGACTGACA
  CGAGACGACTTCCTGGGCCAGGTGGACGTGCCCCTTAGTCACCTTCCGACAGAAGATCCA
  ACCATGGAGCGACCCTATACATTTAAGGACTTTCTCCTCAGACCAAGAAGTCATAAGTCT
  CGAGTTAAGGGATTTTTGCGATTGAAAATGGCCTATATGCCAAAAAATGGAGGTCAAGAT
  GAAGAAAACAGTGACCAGAGGGATGACATGGAGCATGGATGGGAAGTTGTTGACTCAAAT
  GACTCGGCTTCTCAGCACCAAGAGGAACTTCCTCCTCCTCCTCTGCCTCCCGGGTGGGAA
  GAAAAAGTGGACAATTTAGGCCGAACTTACTATGTCAACCACAACAACCGGACCACTCAG
  TGGCACAGACCAAGCCTGATGGACGTGTCCTCGGAGTCGGACAATAACATCAGACAGATC
  AACCAGGAGGCAGCACACCGGCGCTTCCGCTCCCGCAGGCACATCAGCGAAGACTTGGAG
  CCCGAGCCCTCGGAGGGCGGGGATGTCCCCGAGCCTTGGGAGACCATTTCAGAGGAAGTG
  AATATCGCTGGAGACTCTCTCGGTCTGGCTCTGCCCCCACCACCGGCCTCCCCAGGATCT
  CGGACCAGCCCTCAGGAGCTGTCAGAGGAACTAAGCAGAAGGCTTCAGATCACTCCAGAC
  TCCAATGGGGAACAGTTCAGCTCTTTGATTCAAAGAGAACCCTCCTCAAGGTTGAGGTCA
  TGCAGTGTCACCGACGCAGTTGCAGAACAGGGCCATCTACCACCGCCATCAGTGGCCTAT
  GTACATACCACGCCGGGTCTGCCTTCAGGCTGGGAAGAAAGAAAAGATGCTAAGGGGCGC
  ACATACTATGTCAATCATAACAATCGAACCACAACTTGGACTCGACCTATCATGCAGCTT
  GCAGAAGATGGTGCGTCCGGATCAGCCACAAACAGTAACAACCATCTAATCGAGCCTCAG
  ATCCGCCGGCCTCGTAGCCTCAGCTCGCCAACAGTAACTTTATCTGCCCCGCTGGAGGGT
  GCCAAGGACTCACCCGTACGTCGGGCTGTGAAAGACACCCTTTCCAACCCACAGTCCCCA
  CAGCCATCACCTTACAACTCCCCCAAACCACAACACAAAGTCACACAGAGCTTCTTGCCA
  CCCGGCTGGGAAATGAGGATAGCGCCAAACGGCCGGCCCTTCTTCATTGATCATAACACA
  AAGACTACAACCTGGGAAGATCCACGTTTGAAATTTCCAGTACATATGCGGTCAAAGACA
  TCTTTAAACCCCAATGACCTTGGCCCCCTTCCTCCTGGCTGGGAAGAAAGAATTCACTTG
  GATGGCCGAACGTTTTATATTGATCATAATAGCAAAATTACTCAGTGGGAAGACCCAAGA
  CTGCAGAACCCAGCTATTACTGGTCCGGCTGTCCCTTACTCCAGAGAATTTAAGCAGAAA
  TATGACTACTTCAGGAAGAAATTAAAGAAACCTGCTGATATCCCCAATAGGTTTGAAATG
  AAACTTCACAGAAATAACATATTTGAAGAGTCCTATCGGAGAATTATGTCCGTGAAAAGA
  CCAGATGTCCTAAAAGCTAGACTGTGGATTGAGTTTGAATCAGAGAAAGGTCTTGACTAT
  GGGGGTGTGGCCAGAGAATGGTTCTTCTTACTGTCCAAAGAGATGTTCAACCCCTACTAC
  GGCCTCTTTGAGTACTCTGCCACGGACAACTACACCCTTCAGATCAACCCTAATTCAGGC
  CTCTGTAATGAGGATCATTTGTCCTACTTCACTTTTATTGGAAGAGTTGCTGGTCTGGCC
  GTATTTCATGGGAAGCTCTTAGATGGTTTCTTCATTAGACCATTTTACAAGATGATGTTG
  GGAAAGCAGATAACCCTGAATGACATGGAATCTGTGGATAGTGAATATTACAACTCTTTG
  AAATGGATCCTGGAGAATGACCCTACTGAGCTGGACCTCATGTTCTGCATAGACGAAGAA
  AACTTTGGACAGACATATCAAGTGGATTTGAAGCCCAATGGGTCAGAAATAATGGTCACA
  AATGAAAACAAAAGGGAATATATCGACTTAGTCATCCAGTGGAGATTTGTGAACAGGGTC
  CAGAAGCAGATGAACGCCTTCTTGGAGGGATTCACAGAACTACTTCCTATTGATTTGATT
  AAAATTTTTGATGAAAATGAGCTGGAGTTGCTCATGTGCGGCCTCGGTGATGTGGATGTG
  AATGACTGGAGACAGCATTCTATTTACAAGAACGGCTACTGCCCAAACCACCCCGTCATT
  CAGTGGTTCTGGAAGGCTGTGCTACTCATGGACGCCGAAAAGCGTATCCGGTTACTGCAG
  TTTGTCACAGGGACATCGCGAGTACCTATGAATGGATTTGCCGAACTTTATGGTTCCAAT
  GGTCCTCAGCTGTTTACAATAGAGCAATGGGGCAGTCCTGAGAAACTGCCCAGAGCTCAC
  ACATGCTTTAATCGCCTTGACTTACCTCCATATGAAACCTTTGAAGATTTACGAGAGAAA
  CTTCTCATGGCCGTGGAAAATGCTCAAGGATTTGAAGGGGTGGATTAA
  
  Clone variation with respect to NM_015277.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015277 unedited
  TTCGGCACCAGGGAGAACCGGCCGTCCGCGCCGCAGCACAGCCGCTGGGAGCGCCTCAGA
  CCCCGCGCGGGGCGCCGGCTCCATGGCGACCGGGCTCGGGGAGCCGGTCTATGGACTTTC
  CGAAGACGAGGGAGAGTCCCGTATTCTCAGAGTAAAAGTTGTTTCTGGAATTGATCTCGC
  CAAAAAGGACATCTTTGGAGCCAGTGATCCGTATGTGAAACTTTCATTGTACGTAGCGGA
  TGAGAATAGAGAACTTGCTTTGGTCCAGACAAAAACAATTAAAAAGACACTGAACCCAAA
  ATGGAATGAAGAATTTTATTTCAGGGTAAACCCATCTAATCACAGACTCCTATTTGAAGT
  ATTTGACGAAAATAGACTGACACGAGACGACTTCCTGGGCCAGGTGGACGTGCCCCTTAG
  TCACCTTCCGACAGAAGATCCAACCATGGAGCGACCCTATACATTTAAGGACTTTCTCCT
  CAGACCAAGAAGTCATAAGTCTCGAGTTAAGGGATTTTTGCGATTGAAAATGGCCTATAT
  GCCAAAAAATGGAGGTCAAGATGAAGAAAACAGTGACCAGAGGGATGACATGGAGCATGG
  ATGGGAAGTTGTTGACTCAAATGACTCGGCTTCTCAGCACCAAGAGGAACTTCCTCCTCC
  TTCTCTGCCTCCCGGGTGGGAAGAAAAAGTGGACAATNTANGCCGAACTTACTATGTCAA
  CCACAACAACCGGACCACTCAGTGGCACAGACCAAGCCTGATGGACGTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015277 unedited
  NNTTTTACTCTGAACCGCGGCCGCATCTAAGATCGGTTTTTTTTTTTTTTTTTTTAGTTA
  CATAGCATCTAAGCTTTTCTGATCACTACCAGGTAATTTTCAACCAATAAGAAAAAGGAA
  CCAACACTCAGCTCTGTAGAAATCTACCTTCTTTTAGAAACCTCAGGCCTCTGCACCCCT
  TTAGACAACTCATTTCCAACCACAACCTTCCATGGCTTCTCACATGCCAGCCAGGGCAGG
  GTAAAAACGGCAATTCCTTACACCGAAAGGTCCTTTTTATGGAACAAACAGACCTTCCCA
  AACCACAACTTTTTCGGCCTATTGATGACTCGCATGAGATCAAATCTCTAGAGAACCCTG
  CACCGCCCCCACAGGGCCGTGGGATGTCTCACGATCACAGAAGCGTCCCTGGCCCCCCCT
  TCCTTCCACTCCGACTTTACGTTTCTCCCCCTAATCCCCCGCCCGCGCCCCCCCCGGCCC
  CCCGCCCCCACATACCCCCTCTTCCCCCCGCCCCCCCCCCTCCCCCCTCCCGCCCCCCCC
  CCCCCCCCCCTCTCCCGCCCCTCCCTCCCCCCCCTTGCCCCTCCCCCTCACACCGCCCCC
  CCACGTTCCCTCCCGCCCCCACCGTCCTCTCCCCACCCCCCCCACCGCTCACCCGCATCT
  ACCCTCCTCTCACCTCTATCCCCGTTCCTCCTAGCCCCCCCGACAGCTCTCCTATGCTCT
  TTTTTGCCCCCCTCGCGCCCACGCCGCTCCACCACCAACCCGCACCCCACTTGCCGACTG
  CCACCAACATCTCCCCCACCTGACGCCACCCTTCAAAGCCCCGCCCCTCCCCCCTCCGAC
  CCACGCCGCCTCCGCGCCTCCCTCTCCCNCCCCTCCACACCTCAACCCCCCCCCCACCCC
  CCTACCCCGCCCCCACCGTCCCTCACGCCCGCCACCCCACTCCCTCGCTCTCTTCTCACC
  CGCTCTCCCCACCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015277
• ORF Size: 2868 bp
• Insert Size: 5000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_015277.2, NP_056092.2
• RefSeq Size: 4871
• RefSeq ORF: 2868
• Locus ID: 23327
• Domains: C2, HECT, WW
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
  Transcript Variant: This variant (d) lacks an in-frame exon, compared to variant j, resulting in a shorter protein (isoform 3), compared to isoform 1. Variant d is also known as NEDD4-2C6 and NEDL3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.