ASAH1 (AK025211) Human Untagged Clone

CAT#: SC102632

(untagged)-Human cDNA: FLJ21558 fis, clone COL06372

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASAH1
• Synonyms: AC|ACDase|ASAH|PHP|PHP32|SMAPME
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK025211, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTGATAAACATTTTGGATGTGATGTTGGAGAACAATTGCAACCTGCCCAGATGTTCAGCTCGCCCGA
  AGGCTGCCTCCTTGGTGACTTGTTTCATCTTGAGTCTAGAGAGTCCAGGCTGGGGAAGAAAACCACCTCC
  AGCCGGCCGTTCCAGCACAGCCCCACTGGCCAAAGCACACACTCACACAATGAAAACACTGAAACTTTTT
  CTTAAAACAAATTTTTCTCCCATTGTCATGGTTCTGTATATTAACACAAAATTTTCTTTTCTCATAGGAG
  AGATTATTTCATTCAATATTTTTTATGAATTATTTACCATTTGTACTTCAATAGTAGCAGAAGACAAAAA
  AGGTAATTGTGCGCTGAGGGAGGGTGGGCAGCATGAAGCACTACATAAAGAAAAATCTTCTAAATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK025211 unedited
  AGGTTCAGATTAGGNATACCATCATATAGGCGGCCGCGNAATTCGGCACGAGGAAAGTTT
  TATCTTTAAGCTAGCAAAGCAGTAGTGTCATTATTTTAAATAGCCAACAGTACCTTCATT
  TTATGAGATGATTGAGAATTTGTGAAAATCAGTAGTATTTATCAACAAAAGTGGCTAGTT
  TTGTGTGCTGCTTCTTTAAAAAAATCATTTAAAAGATAGCATACTGGAAATATGTACTTG
  CTGCCTTTATGGAAAGCAAGTCTAATTCCTTTATTATGTAAAGAACTCTAGCAACTCAAT
  ACCAAATAGAAATACCTTATGGATTAAGAAGAATAACATGATGTATAAGTGGTGTAAATT
  TATATAATTATTATAAATTATTATAAATGTATAATAAAATAACTAGAATTTCACCAAATT
  AGAAATTTAATGGAAATAAAATACACATTGGATTTGGGTGAAAAAAACATGTTGAGCTTA
  ACATGTTAATGTTAAATGGGACAGAAATACAATGTGCTGCCTACTTTTGAATTAAATGCT
  AAATAGCAGTATTATACATTTTGGGAAAATATTCAAAAAATAAAATACCCTAGAATGGAG
  TTTCTTGGCAGCTCTGTACTAATTTTAATAGTTCTTGAATCTCCTTTTTCACCTTAGAAA
  TTTTAGTCACACTTTAAAAATCTGGGCAAAATGTATGTCCAGCAAACAGCCAAGAGCCAG
  ATGTATTAAATGATCATATTTACCCTCCCAAAAGAAAATTTAAAATGGAAGCCTGTTGAT
  AAACATTTTGCGATGTGATGTTTGGAAACCAATTGCAACCTGGCCCAGATGTTTAACTTC
  GCCCGCAAGCTGCCCTCCTTGTGGACTTGTTTCATTTTAGATCTAAAAAGCCCAGCCTGT
  GGAAAGAAAACACCTCCACCCGGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK025211 unedited
  CTTGCCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGATGGAGTCTG
  GCTCTGTATCCCAAGCTGCAGTGCAGTGGCGCGATCTCGGCTCACTGCAACCTCCGCCTC
  GCAGGTTCAAAAAAGTCTCTGCCTCAGGCTCCCAAGTAGCCGGGATTACAGATGCCCACC
  ACCATGCCTGGCTAATTTTTATGTTTTTAGTAGAGACGGGGTTTCACCATCTTGGCCAGG
  CTGGTCTTGAACTCCTGACCTCGTGATCCACCCGTGTCAGCCTCCCAAAAAGCTGGGATT
  ACAGGCGTGAACCACCATGCCTGACCCTTTGTTCTTTACTTACCCAAGAAATACTCCAAA
  ATCCATGTTTCTCCCATGTATTAGATGACCTATTTGAAGGTAGACAGAAGAGACGTGTAA
  TAGCAGTTAAGATTCTAAATCAAAGCTGGACAATTAGTTTTGATTAATTTATTTATTTTT
  GAGACAGAGTCTCGTTCTGTTGGCCAGACTGGAGTGCAGTGGTGCGATCTCAGCTCACTG
  CGACCTCTGCCTCCCAGGTTCAAGCAATTCTTGTGCCTCAGCCTTCCACGTAGTTGGGAT
  TACAGACATGTGCGACCGCACCTGGCTACTTTTTTTTTTTTTTTGTATTTTTCTTTTAGT
  AGAGAAGGGGTTTTACCATGTTGGCCAGTCTGGTGTCAAACTCCCAACCTCAGATGATTC
  GCCCGCCTCGGCCTCGCAAAGTGCTGGCATTACAGGTGTAAGCCACTGTGCCTGGGCCTC
  TAATTTGATTAATTTTATTCAAACTGTTTCTTCAAACCATAGAGTNAGAACATNCTATAT
  CCTAAGCTTCACANAGGGACTGTTGCACCATCTTCTGCAGATAACCACAAATCTGAAACC
  GGGGTTTCCAGTAGATCAATATTGGAAAGGATCATGGCATGGTTGGGTCCTATCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: AK025211
• Insert Size: 2590 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK025211.1, BAB15085.1
• RefSeq Size: 1774 bp
• RefSeq ORF: 417 bp
• Locus ID: 427
• Cytogenetics: 8p22
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome, Metabolic pathways, Sphingolipid metabolism
• Gene Summary: 'This gene encodes a member of the acid ceramidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. Processing of this preproprotein generates alpha and beta subunits that heterodimerize to form the mature lysosomal enzyme, which catalyzes the degradation of ceramide into sphingosine and free fatty acid. This enzyme is overexpressed in multiple human cancers and may play a role in cancer progression. Mutations in this gene are associated with the lysosomal storage disorder, Farber lipogranulomatosis, and a neuromuscular disorder, spinal muscular atrophy with progressive myoclonic epilepsy. [provided by RefSeq, Oct 2015]'